A. V. Toroptseva, A. A. Markova, M. T. Nguyen, M. I. Abdullina, M. V. Motyakin, O. A. Mayorova, A. V. Bychkova
{"title":"由氧化铁和血清白蛋白组成的纳米系统作为药物递送到细胞的平台：Cy5-HSA@IONPs纳米系统成分在肿瘤细胞积累前后的分析","authors":"A. V. Toroptseva, A. A. Markova, M. T. Nguyen, M. I. Abdullina, M. V. Motyakin, O. A. Mayorova, A. V. Bychkova","doi":"10.1134/S1990747824700557","DOIUrl":null,"url":null,"abstract":"<p>The hybrid shell@core nanosystem Cy5-HSA@IONPs containing magnetic iron oxide core and Cy5-HSA shell was engineered and characterized by composition, stability and integrity using fluorescent measurements, UV/Vis-spectrophotometry (particularly, Bradford protein assay), dynamic light scattering, electron magnetic resonance in solution, in cells and cell lysates. Conjugation of HSA to Cyanine 5 (Cy5) fluorescent dye was carried out for qualitative and quantitative estimations. The research included incubation of hybrid nanosystems with human adenocarcinoma cells (HCT116 and MCF7, colon and breast cell lines, respectively) followed by cell lysis, magnetic separation and quantitative determination of protein on the surface of the magnetic cores after mild cell lysis and in suspension of cell residues. The presence of the albumin coating on the surface of magnetic cores (IONPs) upon delivery to the cells has been proven and the effectiveness of the engineered hybrid particles as a drug (photosensitizer) delivery platform has been discussed.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"19 1","pages":"122 - 126"},"PeriodicalIF":1.1000,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nanosystems Consisting of Iron Oxide and Serum Albumin As a Platform for Drug Delivery into Cells: Analysis of the Cy5-HSA@IONPs Nanosystem Components before and after Accumulation by Tumor Cells\",\"authors\":\"A. V. Toroptseva, A. A. Markova, M. T. Nguyen, M. I. Abdullina, M. V. Motyakin, O. A. Mayorova, A. V. Bychkova\",\"doi\":\"10.1134/S1990747824700557\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The hybrid shell@core nanosystem Cy5-HSA@IONPs containing magnetic iron oxide core and Cy5-HSA shell was engineered and characterized by composition, stability and integrity using fluorescent measurements, UV/Vis-spectrophotometry (particularly, Bradford protein assay), dynamic light scattering, electron magnetic resonance in solution, in cells and cell lysates. Conjugation of HSA to Cyanine 5 (Cy5) fluorescent dye was carried out for qualitative and quantitative estimations. The research included incubation of hybrid nanosystems with human adenocarcinoma cells (HCT116 and MCF7, colon and breast cell lines, respectively) followed by cell lysis, magnetic separation and quantitative determination of protein on the surface of the magnetic cores after mild cell lysis and in suspension of cell residues. The presence of the albumin coating on the surface of magnetic cores (IONPs) upon delivery to the cells has been proven and the effectiveness of the engineered hybrid particles as a drug (photosensitizer) delivery platform has been discussed.</p>\",\"PeriodicalId\":484,\"journal\":{\"name\":\"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology\",\"volume\":\"19 1\",\"pages\":\"122 - 126\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-04-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S1990747824700557\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1134/S1990747824700557","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Nanosystems Consisting of Iron Oxide and Serum Albumin As a Platform for Drug Delivery into Cells: Analysis of the Cy5-HSA@IONPs Nanosystem Components before and after Accumulation by Tumor Cells
The hybrid shell@core nanosystem Cy5-HSA@IONPs containing magnetic iron oxide core and Cy5-HSA shell was engineered and characterized by composition, stability and integrity using fluorescent measurements, UV/Vis-spectrophotometry (particularly, Bradford protein assay), dynamic light scattering, electron magnetic resonance in solution, in cells and cell lysates. Conjugation of HSA to Cyanine 5 (Cy5) fluorescent dye was carried out for qualitative and quantitative estimations. The research included incubation of hybrid nanosystems with human adenocarcinoma cells (HCT116 and MCF7, colon and breast cell lines, respectively) followed by cell lysis, magnetic separation and quantitative determination of protein on the surface of the magnetic cores after mild cell lysis and in suspension of cell residues. The presence of the albumin coating on the surface of magnetic cores (IONPs) upon delivery to the cells has been proven and the effectiveness of the engineered hybrid particles as a drug (photosensitizer) delivery platform has been discussed.
期刊介绍：
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
