Nanosystems Consisting of Iron Oxide and Serum Albumin As a Platform for Drug Delivery into Cells: Analysis of the Cy5-HSA@IONPs Nanosystem Components before and after Accumulation by Tumor Cells

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-04-24 DOI:10.1134/S1990747824700557

A. V. Toroptseva, A. A. Markova, M. T. Nguyen, M. I. Abdullina, M. V. Motyakin, O. A. Mayorova, A. V. Bychkova

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引用次数: 0

Abstract

The hybrid shell@core nanosystem Cy5-HSA@IONPs containing magnetic iron oxide core and Cy5-HSA shell was engineered and characterized by composition, stability and integrity using fluorescent measurements, UV/Vis-spectrophotometry (particularly, Bradford protein assay), dynamic light scattering, electron magnetic resonance in solution, in cells and cell lysates. Conjugation of HSA to Cyanine 5 (Cy5) fluorescent dye was carried out for qualitative and quantitative estimations. The research included incubation of hybrid nanosystems with human adenocarcinoma cells (HCT116 and MCF7, colon and breast cell lines, respectively) followed by cell lysis, magnetic separation and quantitative determination of protein on the surface of the magnetic cores after mild cell lysis and in suspension of cell residues. The presence of the albumin coating on the surface of magnetic cores (IONPs) upon delivery to the cells has been proven and the effectiveness of the engineered hybrid particles as a drug (photosensitizer) delivery platform has been discussed.

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由氧化铁和血清白蛋白组成的纳米系统作为药物递送到细胞的平台：Cy5-HSA@IONPs纳米系统成分在肿瘤细胞积累前后的分析

混合shell@core纳米体系Cy5-HSA@IONPs含有磁性氧化铁核和Cy5-HSA壳，并通过荧光测量、UV/ vis分光光度法（特别是Bradford蛋白测定）、动态光散射、溶液、细胞和细胞裂解物中的电子磁共振，对其组成、稳定性和完整性进行了表征。将HSA与菁5 （Cy5）荧光染料偶联进行定性和定量评价。该研究包括将混合纳米系统与人腺癌细胞（HCT116和MCF7，分别为结肠癌和乳腺癌细胞系）孵育，然后进行细胞裂解，磁分离和定量测定轻度细胞裂解和细胞残留物悬浮后磁芯表面的蛋白质。白蛋白涂层在磁芯（IONPs）表面的存在已被证实，并且工程杂交颗粒作为药物（光敏剂）递送平台的有效性已被讨论。

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来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.