DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis.

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Qian Zhang, Xing Li, Jiahong Geng-Li, Jinyong Yang, Jingyi Liu, Ke Wang, Yuancan Cheng, Jianping Liu, Feiyun Xu, Zhengrui Wang, Kang Zhang, Jianhua Zhang, Feng Cheng, Weifeng Xu, Wei Yuan
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引用次数: 0

Abstract

White lupin exhibits remarkable adaptability to phosphorus (P)-deficient soil through the development of cluster roots (CR), thereby enhancing P use sufficiency. Despite its crucial role, the underlying mechanism governing CR formation remains elusive. Here, we reveal an elevated DNA methylation level through whole-genome bisulfite sequencing in CR in response to P deficiency, particularly in gene and flanking regions, suggesting a responsive epigenetic mechanism. To further investigate the potential involvement of epigenetic remodelling, we treated lupin plants with the DNA methyltransferase (DNMT) inhibitor 5-azacytidine, which led to a disruption of total DNMT activity and impaired CR formation under phosphorus-deficient conditions. Integrated analysis of methylome and RNA-Seq highlights the methylation of CAFFEIC ACID O-METHYLTRANSFERASE 1 (COMT1), a key enzyme in melatonin synthesis, as pivotal for promoting CR formation in white lupin. Functional validation through overexpression or gene silencing of LaCOMT1 in transgenic lupin roots confirms the positive impact of LaCOMT1 on CR formation. Furthermore, melatonin application directly increases CR numbers, indicating the role of methylation-activated LaCOMT1 in promoting CR formation via melatonin synthesis. Those findings provide insights into the epigenomic landscape of white lupin, establishing a direct genetic link between epigenetic mechanisms and P-deficiency-induced CR formation.

DNA甲基化激活的LaCOMT1表达通过褪黑素合成机制促进白露根茎的形成。
白豆通过簇根的发育对缺磷土壤表现出显著的适应性,从而提高了磷的利用充分性。尽管它起着至关重要的作用,但控制CR形成的潜在机制仍然难以捉摸。在这里,我们通过全基因组亚硫酸盐测序揭示了CR中DNA甲基化水平升高,以响应P缺乏,特别是在基因和侧翼区域,提示响应的表观遗传机制。为了进一步研究表观遗传重塑的潜在参与,我们用DNA甲基转移酶(DNMT)抑制剂5-氮杂胞苷处理罗平植物,导致总DNMT活性中断,并在缺磷条件下破坏CR的形成。甲基组和RNA-Seq的综合分析表明,咖啡酸o -甲基转移酶1 (COMT1)的甲基化,褪黑素合成的关键酶,是促进白露CR形成的关键。通过LaCOMT1在转基因罗苹根中过表达或基因沉默的功能验证,证实了LaCOMT1对CR形成的积极影响。此外,褪黑激素的使用直接增加了CR的数量,这表明甲基化激活的LaCOMT1通过褪黑激素的合成促进了CR的形成。这些发现为白豆的表观基因组景观提供了见解,建立了表观遗传机制与缺磷诱导的CR形成之间的直接遗传联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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