Trichoderma koningiopsis Induced Changes in Root Exudates of Masson Pine Seedlings Alter Rhizosphere Microbiome to Enhance Damping-Off Disease Resistance.

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Xuewen Wang, Hongjin Wei, Jiaojiao Lei, Zhibing Rui, Cun Yu
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Abstract

Damping-off disease, primarily caused by Fusarium oxysporum, poses a significant challenge to the cultivation of Masson pine (Pinus massoniana) seedlings. Although Trichoderma koningiopsis improves damping-off disease resistance in Masson pine by regulating the rhizosphere microbial community, the underlying mechanisms remain unknown. Metabolomic analysis showed that T. koningiopsis altered Masson pine root exudates, especially plant organic acids such as capric acid (CA), lauric acid (LA) and pelargonic acid (PA). Co-culturing rhizosphere microbes with 0.1 mM CA, LA, PA and a combination of the three (1:1:1, CDNs1) significantly inhibited F. oxysporum and promoted the growth of rhizosphere biocontrol strains (Trichoderma, Penicillium and Bacillus), with CDNs1 exerting a superior effect. Amplicon sequencing and RT-qPCR showed that CDNs1 significantly altered the microbial community composition in the rhizosphere, especially inhibited the growth of Fusarium and enriched beneficial microbes (Trichoderma and Penicillium). CDNs1 effectively decreased the incidence and severity index of damping-off disease in Masson pine seedlings by 73.33% and 41.67%, respectively. Mechanistically, CDNs1 enhanced resistance to damping-off disease by modulating plant hormones, oxidative stress defences and the photosynthesis pathway. Collectively, this study provides insight into the mechanism by which T. koningiopsis enhances damping-off disease resistance by regulating the rhizosphere microbial community.

康宁木霉诱导马尾松幼苗根系分泌物改变根际微生物群以增强抗湿性。
以尖孢镰刀菌(Fusarium oxysporum)为主要病原菌的消湿病是马尾松苗木栽培面临的重大挑战。虽然koningiopsis木霉通过调节根际微生物群落提高马尾松的抗病性,但其潜在机制尚不清楚。代谢组学分析表明,koningiopsis改变了马尾松根系分泌物,特别是植物有机酸,如癸酸(CA)、月桂酸(LA)和天葵酸(PA)。0.1 mM CA、LA、PA和三者(1:1:1,CDNs1)共培养根际微生物,显著抑制尖孢镰刀菌,促进根际生物防治菌株(木霉、青霉和芽孢杆菌)的生长,其中CDNs1效果更佳。扩增子测序和RT-qPCR结果显示,CDNs1显著改变了根际微生物群落组成,特别是抑制了镰刀菌的生长,并富集了有益微生物(木霉和青霉)。CDNs1可有效降低马尾松幼苗灭湿病发生率和严重程度指数,分别降低73.33%和41.67%。从机制上讲,CDNs1通过调节植物激素、氧化应激防御和光合作用途径来增强对抑制疾病的抵抗力。总的来说,这项研究提供了洞见的机制,通过koningiopsis通过调节根际微生物群落增强抗病性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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