Unravelling the Significance of Phosphoenolpyruvate Carboxylase in Phosphate Starvation Responses.

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Jesús Pérez-López, Clara de la Osa, Jacinto Gandullo, Nora Gigli-Bisceglia, Inmaculada Coleto, Ana Belén Feria, Cristina Echevarría, Christa Testerink, Daniel Marino, Sofía García-Mauriño, José A Monreal
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Abstract

Low phosphate availability is a major concern for agriculture. Plants develop a plethora of responses to improve phosphate acquisition, known as phosphate starvation responses (PSR). Among them, the induction of phosphoenolpyruvate carboxylase (PEPC) has been described in many plants. However, most studies have been conducted in the absence of phosphate, thus the real impact of PEPC in PSR is missing as there is no phosphate to take up. In this study, we used modified sorghum plants silenced in the main PEPC isozyme in roots, SbPPC3, and analyzed the role of PEPC in the presence of insoluble calcium phosphate (PCa), showing a phosphate starvation phenotype in silenced but not in WT plants. Interestingly, root exudation of citrate was not reduced in silenced plants, probably due to a higher citrate synthase activity, but it was reduced for succinate, another compound with phosphate solubilisation capacity. Finally, silenced plants accumulated less P in roots with PCa, leading to a reduced phosphate acquisition efficiency (PAE). Our results show, for the first time, the actual role of PEPC in phosphate solubilisation through succinate exudation, proposing PPC3 as a specific target to improve PAE in plants.

揭示磷酸烯醇丙酮酸羧化酶在磷酸盐饥饿反应中的意义。
磷酸盐利用率低是农业的一个主要问题。植物产生了大量的反应来改善磷酸盐的获取,称为磷酸盐饥饿反应(PSR)。其中,磷酸烯醇丙酮酸羧化酶(PEPC)的诱导作用在许多植物中都有报道。然而,大多数研究都是在没有磷酸盐的情况下进行的,因此PEPC在PSR中的真正影响是缺失的,因为没有磷酸盐可以吸收。在本研究中,我们以根中主要PEPC同工酶SbPPC3沉默的转基因高粱植株为研究对象,分析了PEPC在不溶性磷酸钙(PCa)存在下的作用,结果显示,在沉默的高粱植株中存在磷酸盐饥饿表型,而在WT植株中没有。有趣的是,沉默植株的根分泌量没有减少,可能是由于较高的柠檬酸合成酶活性,但琥珀酸盐(另一种具有磷酸盐溶解能力的化合物)的根分泌量减少了。最后,沉默植株在PCa作用下根系积累的磷减少,导致磷获取效率(PAE)降低。我们的研究结果首次表明,PEPC在通过琥珀酸盐渗出的磷酸盐增溶中的实际作用,提出PPC3是改善植物PAE的特定靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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