小角中子散射和Alphafold2预测揭示非结构蛋白8的RNA结合敏感性。

IF 16 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

ACS Nano Pub Date : 2025-10-17 DOI:10.1021/acsnano.4c16790

Xin Jiang,Jinxin Xu,Zhenyu Liao,Na Wang,Taisen Zuo,Changli Ma,Hanqiu Jiang,Yubin Ke,He Cheng,Howard Wang,Jinkui Zhao,Jun Fan,Jinsong Liu,Xiangqiang Chu

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引用次数: 0

摘要

这种灵活的结构使非结构蛋白8 （nsp8）能够快速响应环境变化，这对SARS-CoV-2的RNA复制和转录至关重要。本文应用小角中子散射和AlphaFold2预测技术对SARS-CoV-2 nsp8二聚体和四聚体的结构变化进行了表征。结果表明，核心结构暴露较多的nsp8四聚体具有较低的热稳定性。暴露的核心结构域增加了对RNA的敏感性，并调整了其结构以与RNA相互作用。我们的工作揭示了两种形式的SARS-CoV-2 nsp8s在RNA合成过程中的结构差异，部分阐明了RNA病毒RNA复制的分子机制。

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RNA Binding Sensitivity of Nonstructural Protein 8 Revealed by Small-Angle Neutron Scattering and Alphafold2 Prediction.

The flexible structure enables nonstructural protein 8 (nsp8) to respond quickly to environmental changes, which are essential for RNA replication and transcription of SARS-CoV-2. In this work, small-angle neutron scattering and AlphaFold2 prediction were applied to characterize the structural change of SARS-CoV-2 nsp8 dimers and tetramers. The results demonstrated that the nsp8 tetramer with a more exposed core domain shows a low thermal stability. The exposed core domain increases its sensitivity to RNA and adapts its structure to interact with RNA. Our work reveals the structural difference between the two forms of SARS-CoV-2 nsp8s in the RNA synthesis process, which partly elucidates the molecular mechanism behind RNA replication of the RNA virus.

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来源期刊

ACS Nano 工程技术-材料科学：综合

CiteScore

26.00

自引率

4.10%

发文量

1627

审稿时长

1.7 months

期刊介绍： ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.