塑料棒尖端的生物催化：相邻裸工作电极盘上的非典型电化学酶生物传感

IF 3.6 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst Pub Date : 2025-05-29 DOI:10.1039/d5an00510h

H. Sasimali M. Soysa, Kawin Loha, Wipa Suginta, Albert Schulte

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引用次数: 0

摘要

葡萄糖氧化酶（GOx）持续固定在PVC棒的扁平尖端上，并在电化学（EC）细胞中使用匹配的电极组进行葡萄糖分析。创新的“粘上酶”概念节省了资源，因为工作电极（WEs）是空的，因此不需要适应技能，WE和固定创新可以自主进行。定制的PVC棒池，每个都用特定的酶修饰，可以根据棒的底物特异性，作为易于获取和可重复使用的快速分析物切换源。考虑到可用的氧化还原酶的范围，“粘上酶”策略意味着广泛的潜在适用性。

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Biocatalysis on Plastic Stick Tips: Atypical Electrochemical Enzyme Biosensing on Adjacent Bare Working Electrode Disks

Glucose oxidase (GOx) was sustainably immobilized on the flattened tips of PVC sticks, and achieved glucose analysis in electrochemical (EC) cells with a matching electrode set. The innovative ‘enzyme-on-a-stick’ concept is resource-saving, as working electrodes (WEs) are left bare, so skills in their adaptation are unnecessary and WE and immobilization innovations can be made autonomously. Tailored pools of PVC sticks, each modified with a particular enzyme, can serve as a readily accessible and reusable source for rapid analyte switching, according to a stick’s substrate specificity. The ‘enzyme on-a-stick’ tactic implies a wide potential applicability, given the range of redox enzymes available.

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