胶体金免疫层析法现场检测植物油中的铅

IF 3.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst Pub Date : 2025-06-02 DOI:10.1039/d5an00393h

Jiaxun Li, liuping zhang, guoping qian, aihong wu, Liqiang Liu, Lingling Guo, lili cheng, Chuanlai Xu, Hua Kuang

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引用次数: 0

摘要

植物油中的铅污染对人体健康构成重大威胁，传统的检测方法存在诸多局限性。本研究制备了一种针对Pb 2 +的单克隆抗体（mAb），并开发了一种胶体金免疫层析法（CGIA）条带，用于植物油中Pb 2 +的现场检测。合成了Pb 2 + -ITCBE-KLH和Pb 2 + -ITCBE-BSA抗原，并用紫外分光光度法对其进行了表征。获得的IgG2a重链和Kappa轻链亚型单抗具有7.3×10⁸L/mol的高亲和力常数和高特异性。通过对胶体金标记的抗pb 2 + mAb的pH值、再悬浮液中的组分以及样品前处理条件的优化，制备的CGIA试纸条对植物油样品的检出限为0.209 mg/kg。加标样品的CGIA回收率为95.78 ~ 98.52%，与电感耦合等离子体质谱法（ICP-MS）的回收率一致，具有较高的可靠性。该CGIA条带为植物油中Pb 2 +的检测提供了一种快速可靠的方法，在确保植物油质量安全方面具有实际应用潜力。

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A Colloidal Gold Immunochromatographic Assay for On-site Lead Detection in Vegetable Oil

Lead (Pb) contamination in vegetable oil poses a significant threat to human health, and traditional detection methods have many limitations. In this study, a monoclonal antibody (mAb) against Pb²⁺ was prepared, and a colloidal gold immunochromatographic assay (CGIA) strip was developed for the on-site detection of Pb²⁺ in vegetable oil. The antigens Pb²⁺-ITCBE-KLH and Pb²⁺-ITCBE-BSA were synthesized and characterized by UV spectrophotometry. The obtained mAb, of IgG2a heavy chain and Kappa light chain subtype, had a high affinity constant of 7.3×10⁸ L/mol and high specificity. Through optimization of the pH of the colloidal gold- labeled anti-Pb²⁺ mAb, components in the resuspension solution, and sample pretreatment conditions, the developed CGIA test strip showed a limit of detection (LOD) of 0.209 mg/kg for vegetable oil samples. The recovery of CGIA for spiked samples was 95.78-98.52%, consistent with that of inductively-coupled plasma mass spectrometry (ICP-MS), indicating its reliability. This CGIA strip provided a rapid and reliable method for detecting Pb²⁺ in vegetable oil, with potential for practical applications in ensuring vegetable oil quality and safety.

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