IF 3.6 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00738d

Malinda Salim, Andrew J Clulow, Jitraporn Vongsvivut, Alastair K H MacGibbon, Ben J Boyd

{"title":"Label-free imaging of milk lipid digestion using synchrotron FTIR micro-spectroscopy.","authors":"Malinda Salim, Andrew J Clulow, Jitraporn Vongsvivut, Alastair K H MacGibbon, Ben J Boyd","doi":"10.1039/d6an00738d","DOIUrl":"10.1039/d6an00738d","url":null,"abstract":"Understanding the behaviour of lipids during digestion of food and formulations can enable more rational design of lipid-based materials to enhance the oral delivery of drugs and nutrients. Traditionally, light microscopy and electron microscopy have been used to 'watch' digestion at the droplet level, while small angle X-ray scattering has more recently given structural insights at the ensemble level, however none of these techniques provide information on local changes in chemical composition of a digesting fat droplet. In this study, we used synchrotron Fourier transform infrared microspectroscopy (s-FTIRM) as a chemical imaging technique to map the distributions of milk components and their digestion products in individual fat globules to obtain a better understanding of the interfacial characteristics and chemical specificity behind the structural changes that occur during digestion. The results showed localisation of fatty acids at the core and the oil/water interface of digesting fat globules and the presence of fatty acid-calcium soaps, which exist both as isolated aggregates and are partially accumulated at the interface. This proof-of-concept study highlights the novel application of s-FTIRM as a label-free, chemically-specific imaging tool to resolve the spatial distribution of digestion products, such as free fatty acids and fatty acid-calcium soaps, within and around individual milk fat globules. This approach provides new insights into the localised chemical changes during lipid digestion at the single droplet level.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":"5449-5458"},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric discrimination of penicillamine enantiomers with a core-shell structured mesoporous chiral SiO₂@MnO₂ nanozyme. 核壳结构介孔手性SiO2@MnO2纳米酶对青霉胺对映体的比色鉴别。

IF 3.6 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00594b

Shuyu Li, Fan Wu, Haowei Huang, Datong Wu, Junyao Li, Wenrong Cai, Yong Kong

引用次数: 0

A dual-mode nano-microelectrode for PTH detection at the single-cell level. 用于单细胞水平PTH检测的双模纳米微电极。

IF 3.6 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00700g

Zhixun Jiang, Kehong Liu, Yingjing Yang, Qincong Feng, Xuejun Liang

{"title":"A dual-mode nano-microelectrode for PTH detection at the single-cell level.","authors":"Zhixun Jiang, Kehong Liu, Yingjing Yang, Qincong Feng, Xuejun Liang","doi":"10.1039/d6an00700g","DOIUrl":"10.1039/d6an00700g","url":null,"abstract":"Parathyroid hormone (PTH) is essential for calcium-phosphate homeostasis and bone metabolism. Abnormal PTH levels are closely associated with chronic kidney disease and several types of cancer. Current mainstream immunological PTH detection methods cannot achieve single-cell analysis, and dual-signal detection strategies are scarce. Herein, we designed a dual-signal nano-microelectrode for single-cell PTH detection by modifying gold-deposited nanopipettes with ferrocene (Fc)-labeled DNA-complementary DNA double strands and 6-mercaptohexanol (6-MCH). Under optimized conditions, square-wave voltammetry (SWV) showed a linear response to PTH in the range of 1-500 pg mL-1 (R2 = 0.99), and EIS exhibited linearity in the range of 1-1000 pg mL-1 (R2 = 0.98). The sensor demonstrated high selectivity against common intracellular interferents and good electrode-to-electrode consistency. Using three-dimensional micro-manipulation technology, minimally invasive detection of individual cells was achieved, and significant changes in electrochemical signals before and after cell penetration were revealed. Furthermore, the intracellular PTH concentration gradually decreased with prolonged extracellular Ca2+ incubation, consistent with the regulatory mechanism of calcium-sensing receptors. This dual-mode nano-micro electrode is a new type of tool that enables rapid, low-cost, label-free and highly specific single-cell PTH analysis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":"5399-5406"},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemiluminescence biosensor for sulfadimethoxine detection based on enzymatic DNA walker-activated CRISPR/Cas12a cascade for signal enhancement. 基于酶促DNA步行者激活的CRISPR/Cas12a级联信号增强的磺胺二甲氧嘧啶检测电化学发光生物传感器。

IF 3.6 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00344c

Guangrui Zou, Jiamin Xie, Minjin Wu, Xiaoping Wei, Jianping Li

{"title":"An electrochemiluminescence biosensor for sulfadimethoxine detection based on enzymatic DNA walker-activated CRISPR/Cas12a cascade for signal enhancement.","authors":"Guangrui Zou, Jiamin Xie, Minjin Wu, Xiaoping Wei, Jianping Li","doi":"10.1039/d6an00344c","DOIUrl":"10.1039/d6an00344c","url":null,"abstract":"Sulfadimethoxine (SDM) is a widely used veterinary antibiotic. Its residues in food and the environment may cause bacterial resistance and threaten human health. In this study, a novel electrochemiluminescence (ECL) biosensor with signal amplification was developed for the sensitive detection of SDM. A g-C3N4@Au composite was modified on the electrode surface, generating a strong ECL signal with K2S2O8 as the coreactant. Hairpin DNA terminated with ferrocene carboxylic acid (FcA) was self-assembled onto g-C3N4@Au via thiol-gold bonds, causing Fc to quench the electrochemiluminescence signal of g-C3N4. Upon SDM addition, it specifically bound to the aptamer strand, releasing the DNA Walker. The DNA walker then continuously hybridized with the activator strand S1 and was cleaved by the nicking endonuclease Nt.BsmAI, generating a large number of S1. S1 further activated the trans-cleavage activity of CRISPR/Cas12a, which cleaves the hairpin DNA on the electrode, releasing Fc and recovering the ECL signal. By monitoring the ECL intensity change during the \"signal-off\" to \"signal-on\" transition, quantitative detection of SDM was indirectly realized. The detection range for SDM is 1.0 × 10-14 to 1.0 × 10-7 mol L-1, with a detection limit of 7.15 × 10-15 mol L-1. The sensor was successfully applied to the detection of SDM in food and water samples. Benefiting from the catalytic cascade amplification involving multiple enzymes, the developed method demonstrated high ultrahigh sensitivity.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":"5335-5343"},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surfactant-associated proteins perturb lipid membrane architecture: insights from nanoplasmonic sensing and differential scanning calorimetry. 表面活性剂相关蛋白扰乱脂质膜结构：来自纳米等离子体传感和差示扫描量热法的见解。

IF 3.6 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00532b

Giacomo Russo, Shishir Jaikishan, Dumidu Perera, Susanne K Wiedmer

{"title":"Surfactant-associated proteins perturb lipid membrane architecture: insights from nanoplasmonic sensing and differential scanning calorimetry.","authors":"Giacomo Russo, Shishir Jaikishan, Dumidu Perera, Susanne K Wiedmer","doi":"10.1039/d6an00532b","DOIUrl":"10.1039/d6an00532b","url":null,"abstract":"This study investigates the biophysical interactions between pulmonary surfactant proteins (SP) and model lipid membranes. Using a combination of state-of-the-art nanoplasmonic sensing (NPS) and micro differential scanning calorimetry (MicroCal DSC), the effects of surfactant protein B (SP-B), surfactant protein C (SP-C), and their mixture on the structural and thermodynamic properties of liposomes composed of neutral phosphatidylcholines and anionic phosphatidylglycerol, (POPC : POPG and DPPC : DMPG, 80 : 20 molar ratio) were assessed. In a first-of-its-kind application for this system, NPS measurements provided real-time, label-free monitoring of the mass-sensing shifts associated with protein-lipid binding and membrane reorganization. Our results suggest that while SP-B primarily influences inter-membrane connectivity and surface-level interactions, SP-C penetrates deeper into the hydrophobic core. The combined SP-B and SP-C system exhibited non-additive effects, affecting the fluidity and stability of the surfactant model. Complementary MicroCal DSC analysis revealed that protein reconstitution into lung-mimicking lipid membranes is a kinetic process requiring extended timeframes to establish stable protein-lipid interactions. The inclusion of 2% (protein-to-lipid ratio) of each SP (and their equimolar mixtures) significantly modulated the thermotropic phase behavior of the anionic bilayers. These changes were characterized by a significant elevation in the gel-to-liquid crystalline transition temperature (Tm) and a concomitant broadening of the main transition endotherm. These thermotropic shifts suggest enhanced protein-lipid interactions, resulting in reduced transition enthalpy (ΔH) and attenuated molecular cooperativity within the lipid bilayer. Together, these findings provide critical insights into the molecular mechanisms by which these proteins maintain lung function, potentially offering a framework for the development of advanced synthetic surfactant therapies.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":"5354-5365"},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148676159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paper-based fluorescent sensor based on molecularly imprinted polymers and upconversion nanoparticles for selective and sensitive D-proline detection in human serum and saliva. 基于分子印迹聚合物和上转换纳米颗粒的纸基荧光传感器用于人血清和唾液中d -脯氨酸的选择性和敏感性检测。

IF 3.6 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00696e

Akarapong Prakobkij, Jiajia Zhou, Mahnaz Maddahfar, Somkid Pencharee, Daniel Citterio, Purim Jarujamrus

{"title":"Paper-based fluorescent sensor based on molecularly imprinted polymers and upconversion nanoparticles for selective and sensitive D-proline detection in human serum and saliva.","authors":"Akarapong Prakobkij, Jiajia Zhou, Mahnaz Maddahfar, Somkid Pencharee, Daniel Citterio, Purim Jarujamrus","doi":"10.1039/d6an00696e","DOIUrl":"10.1039/d6an00696e","url":null,"abstract":"D-Proline has been reported as a potential biomarker for gastric (stomach) cancer, creating a demand for practical and rapid analytical approaches for early diagnosis using simple and non-invasive clinical samples. In this work, a microfluidic paper-based analytical device (µPAD) integrating molecularly imprinted polymer-functionalized upconversion nanoparticles (UCNPs@MIPs) was developed for the selective fluorescence-based detection of D-proline. Owing to near-infrared excitation (980 nm), UCNPs provide background-free fluorescence with high photostability, while MIPs serve as artificial receptors to impart molecular selectivity. The sensing mechanism relies on fluorescence quenching of UCNPs@MIPs induced by D-proline through multiphonon relaxation, resulting in an inverse relationship between fluorescence intensity and analyte concentration. Quantitative analysis was achieved using a homemade IR-laser-controlled light box and smartphone-based image capture, with signal evaluation based on blue-channel intensity. The proposed platform exhibited a linear response over the concentration range of 0.01-0.30 mM, with a limit of detection of 0.016 mM. The method was successfully applied to the determination of spiked D-proline in human saliva and serum samples. Owing to its simplicity, low sample consumption, portability, and adaptability, the proposed sensing strategy shows strong potential for on-site analysis and can be extended to broader applications in chemical analysis, biosensing, and environmental monitoring.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":"5286-5298"},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148596616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Styryl pyridinium fluorophores with sequential plasma membrane-to-mitochondria targeting and application to sulphite sensing in live cells. 具有序贯质膜-线粒体靶向的苯乙烯基吡啶荧光团及其在活细胞亚硫酸盐传感中的应用。

IF 3.6 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00871b

Shabnam Mansuri, Paramasivam Mahalingam, Prateek Sarkar, Gaurav Rawat, Yousuke Katsuda, Sriram Kanvah

{"title":"Styryl pyridinium fluorophores with sequential plasma membrane-to-mitochondria targeting and application to sulphite sensing in live cells.","authors":"Shabnam Mansuri, Paramasivam Mahalingam, Prateek Sarkar, Gaurav Rawat, Yousuke Katsuda, Sriram Kanvah","doi":"10.1039/d6an00871b","DOIUrl":"10.1039/d6an00871b","url":null,"abstract":"The development of fluorescent probes that integrate spatiotemporal organelle targeting with analyte sensing remains a significant challenge. Herein, we report a modular library of four styryl pyridinium fluorophores (Py-Cz-CN, Py-Cz, Py-Am-CN, and Py-Am) based on a donor-π-acceptor (D-π-A) architecture for sequential organelle targeting and sulfite sensing. All four probes exhibited rapid and selective plasma membrane localization, with high colocalization coefficients (PCC = 0.81-0.88). Notably, Py-Cz underwent spontaneous time-dependent translocation from the plasma membrane to mitochondria over 6 h, with the Pearson's correlation coefficient increasing from 0.15 to 0.87, and this mitochondrial accumulation was confirmed to be ΔΨm-dependent by CCCP-induced membrane depolarization. In contrast, cyano substitution at the vinyl bridge preserved plasma membrane localization while introducing a Michael acceptor for selective reaction-based fluorescence turn-off sensing of bisulfite/sulfite. Py-Cz-CN exhibited high sensitivity toward bisulfite (LOD = 5.9 μM) and enabled both exogenous and endogenous sulfite imaging in live cells. DFT and TDDFT calculations further supported the excited-state characteristics and sensing mechanism. This work establishes a simple molecular design strategy for combining organelle-specific trafficking with analyte-responsive fluorescence in multifunctional small-molecule probes.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":"5424-5438"},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a carbon quantum dot-based mixed-mode stationary phase and its application in reversed-phase/hydrophilic interaction chromatography. 基于碳量子点的混合模式固定相的研制及其在反相/亲水性相互作用色谱中的应用。

IF 3.6 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00677a

Shuxian Zu, Yiwen Zhang, Yingtong Ma, Jinyan Fan, Xiaowan Zhang, Yang Liu, Longshan Zhao

{"title":"Development of a carbon quantum dot-based mixed-mode stationary phase and its application in reversed-phase/hydrophilic interaction chromatography.","authors":"Shuxian Zu, Yiwen Zhang, Yingtong Ma, Jinyan Fan, Xiaowan Zhang, Yang Liu, Longshan Zhao","doi":"10.1039/d6an00677a","DOIUrl":"10.1039/d6an00677a","url":null,"abstract":"The development of mixed-mode chromatography (MMC) is a research hotspot in the field of separation science, and carbon quantum dots (CQDs) show remarkable potential in the development of stationary phases for chromatographic columns due to their excellent properties. In this study, amphiphilic CQDs with both hydrophilic groups and hydrophobic long-chain alkyl groups were synthesized from mercaptopropionic acid and 1,12-diaminododecane, and bonded with silica gel to obtain the column stationary phase Sil-CQDs. The stationary phase effectively separated hydrophobic and hydrophilic compounds such as alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs), anilines, nucleoside bases, sulfonamides, and thioureas in both reversed-phase liquid chromatography (RPLC) and hydrophilic interaction chromatography (HILIC) modes. The impacts of chromatographic conditions on the retention behaviour of analytes were investigated. Additionally, the Sil-CQDs column showed good separation results in comparison with the Sil-Cl column without CQDs. It also exhibited outstanding stability and reproducibility, satisfying the needs for practical analysis. This work offers a novel approach for the separation and analysis of complicated materials as well as an innovative idea for the use of CQDs in mixed-mode chromatography.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":"5490-5497"},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A highly sensitive and specific fluorescent immunoassay for hepatitis B envelope antigen detection using AIE-encoded nanobioprobes. 使用aie编码的纳米生物探针进行乙型肝炎包膜抗原检测的高灵敏度和特异性荧光免疫分析。

IF 3.6 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00745g

Yixin Shi, Xinxi Lu, Yifang Li, Youlang Cao, Ling-Hong Xiong, Xuewen He

{"title":"A highly sensitive and specific fluorescent immunoassay for hepatitis B envelope antigen detection using AIE-encoded nanobioprobes.","authors":"Yixin Shi, Xinxi Lu, Yifang Li, Youlang Cao, Ling-Hong Xiong, Xuewen He","doi":"10.1039/d6an00745g","DOIUrl":"10.1039/d6an00745g","url":null,"abstract":"Developing highly sensitive, efficient, stable, and low-cost viral antigen detection strategies suitable for primary healthcare settings is of great significance for the early diagnosis and treatment of viral infections. Herein, we report a highly efficient fluorescent immunoassay strategy for the detection of hepatitis B envelope antigen (HBeAg) based on aggregation-induced emission (AIE) molecule-encoded fluorescent nanospheres. Three AIE luminogens (TPE-Br4, TPE-O2, and TPE-O3) were encapsulated into carboxyl-functionalized nanospheres via an ultrasonic swelling method, yielding uniform and well-dispersed multicolor fluorescent nanospheres. Among them, the red-emitting nanospheres, exhibiting superior photostability, were selected as signal output units. Anti-hepatitis B envelope antibodies were subsequently conjugated onto their surfaces to construct fluorescent nanobioprobes. By integrating these nanobioprobes with a sandwich-type enzyme-linked immunosorbent assay, specific capture and recognition of HBeAg were successfully achieved. The proposed method demonstrated a linear detection range of 0.01-1 ng mL-1 and an ultra-low limit of detection (LOD) of 9 pg mL-1. In addition, it exhibited excellent specificity and robust anti-interference performance in spiked fresh mouse serum samples. Overall, this work presents a sensitive, convenient, and cost-effective sensing platform for HBeAg detection, showing great potential for rapid and on-site screening of viral antigens in clinical applications.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":"5258-5266"},"PeriodicalIF":3.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148589825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Multipole Ion Guide Transmission Performance under Consistent Benchmark Conditions 一致基准条件下多极离子波导传输性能的比较分析

IF 4.2 3区化学

Analyst Pub Date : 2026-09-16 DOI: 10.1039/d6an00949b

Tianle Yang,Zhongjun Zhao,Yanting Yang,Ziqiu Su,Xing Guo,Yixiang Duan

{"title":"Comparative Analysis of Multipole Ion Guide Transmission Performance under Consistent Benchmark Conditions","authors":"Tianle Yang,Zhongjun Zhao,Yanting Yang,Ziqiu Su,Xing Guo,Yixiang Duan","doi":"10.1039/d6an00949b","DOIUrl":"https://doi.org/10.1039/d6an00949b","url":null,"abstract":"Radio-frequency (RF) multipole ion guides transfer ions between pressure stages in mass spectrometers, but comparisons of pole number are commonly entangled with changes in aperture, guide length, and instrument layout. We compared quadrupole, hexapole, and octopole guides in SIMION using the same inscribed radius (r 0 = 5.24 mm), axial length, mass grid, and response definitions. Interchangeable quadrupole and hexapole cartridges were also evaluated in the same ion-guide position of a laboratory-built electrospray ionization time-of-flight mass spectrometer (ESI-TOFMS); the octopole served as the numerical extension of the matched guide-order benchmark. Across the sampled amplitude grid, mass-averaged transmission over m/z 50-2000 was greatest at 300 V for the quadrupole and 200 V for both higher-order guides. In the separate frequency scan, the best sampled settings were 1.0, 1.5, and 2.0 MHz, with pointwise maximum transmission efficiencies of 56%, 65%, and 79%, respectively. Increasing pole number broadened the simulated transmission window but increased the root-mean-square (RMS) exit beam radius. Among the 35 masses reported for all three guides at 1 and 2 Pa, the pressure increase reduced the mean RMS exit radius by 51.8%, 32.7%, and 29.1%. In the experimental RF-amplitude scans, the maximum mean peak area for the hexapole was 1.33-2.78 times the corresponding quadrupole maximum across five diagnostic ions; the response also varied with inscribed radius and m/z. The matched benchmark therefore resolves a practical design trade-off: higher pole number expands the transmitted mass range, whereas lower pole number gives tighter exit-beam confinement.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"37 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148964449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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