Analyst最新文献

{"title":"A novel biosensor for highly sensitive DNA damage detection using TdT and CRISPR-Cas12a†","authors":"Juan Wang, Fan Zhang, Ziyang Liu, Yue Zhou, Liguo Pei and Bei Yan","doi":"10.1039/D5AN00031A","DOIUrl":"10.1039/D5AN00031A","url":null,"abstract":"<p >This study presents a highly sensitive fluorescence biosensor integrating TdT enzyme and CRISPR-Cas12a for precise detection of sperm DNA damage, achieving a detection limit of 0.99 pM and a linear range of 0.001–0.2 nM. Its exceptional sensitivity, simplicity, and versatility make it a transformative tool for reproductive medicine and clinical diagnostics.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1076-1080"},"PeriodicalIF":3.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in metallic and metal oxide nanoparticle-assisted molecular methods for the detection of Escherichia coli","authors":"Linlin Zhuang, Jiansen Gong, Di Zhang, Ping Zhang, Ying Zhao, Li Sun, Jianbo Yang, Yu Zhang and Qiuping Shen","doi":"10.1039/D4AN01495B","DOIUrl":"10.1039/D4AN01495B","url":null,"abstract":"<p >The detection of <em>E. coli</em> is of irreplaceable importance for the maintenance of public health and food safety. In the field of molecular detection, metal and metal oxide nanoparticles have demonstrated significant advantages due to their unique physicochemical properties, and their application in <em>E. coli</em> detection has become a cutting-edge focus of scientific research. This review systematically introduces the innovative applications of these nanoparticles in <em>E. coli</em> detection, including the use of magnetic nanoparticles for efficient enrichment of bacteria and precise purification of nucleic acids, as well as a variety of nanoparticle-assisted immunoassays such as enzyme-linked immunosorbent assays, lateral flow immunoassays, colorimetric methods, and fluorescence strategies. In addition, this paper addresses the application of nanoparticles used in nucleic acid tests, including amplification-free and amplification-based assays. Furthermore, the application of nanoparticles used in electrochemical and optical biosensors in <em>E. coli</em> detection is described, as well as other innovative assays. The advantages and challenges of the aforementioned technologies are subjected to rigorous analysis, and a prospective outlook on the future direction of development is presented. In conclusion, this review not only illustrates the practical utility and extensive potential of metal and metal oxide nanoparticles in <em>E. coli</em> detection, but also serves as a scientific and comprehensive reference for molecular diagnostics in food safety and public health.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 7","pages":" 1206-1228"},"PeriodicalIF":3.6,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygen vacancy-enriched NiO nanozymes achieved via facile annealing in argon for detection of l-Cys†","authors":"Sihua Wu, Jinhui Zou, Baohua Zhang, Jiantian Lu, Guanrong Lin, Yuwei Zhang and Li Niu","doi":"10.1039/D5AN00054H","DOIUrl":"10.1039/D5AN00054H","url":null,"abstract":"<p >Nickel oxide (NiO) nanozymes, as an excellent oxidase mimic, have been widely used in fluorescence biological detection, water pollutant analysis, food safety and cell imaging. However, a great challenge in fully realising these applications is regulating their crystalline micro-/nano-structure and composites to achieve high enzyme activity and high specific surface area. Herein, we applied a very simple thermal annealing treatment to restructure the calcined precursor of NiO. Importantly, it was found that the oxygen vacancy (O<small>_V</small>) concentration of the targeted NiO nanozyme significantly increased when the annealing atmosphere was argon rather than air. Moreover, the as-prepared novel NiO sample (NiO-O<small>_V</small>) nanosheets achieved about 2-fold enhancement in their specific surface area. It is believed that a higher O<small>_V</small> concentration and larger specific surface area increase enzyme activity by accelerating the electron transfer rate and improving catalytic interfaces. The significant improvement in the enzyme activity of NiO-O<small>_V</small> was verified using the fluorescence “turn-on” experiment of Amplex Red (AR). Finally, using the NiO-O<small>_V</small>/AR system, we constructed a highly sensitive enzyme sensor on <small>L</small>-Cys with a detection limit of 37.8 nM. The sensor also displayed excellent specificity for ten typical amino acid interferents.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 7","pages":" 1338-1346"},"PeriodicalIF":3.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating sample normalization methods for MS-based multi-omics and the application to a neurodegenerative mouse model†","authors":"Gwang Bin Lee, Cha Yang, Fenghua Hu and Ling Hao","doi":"10.1039/D4AN01573H","DOIUrl":"10.1039/D4AN01573H","url":null,"abstract":"<p >Mass spectrometry (MS)-based omics methods have transformed biomedical research with accurate and high-throughput analysis of diverse molecules in biological systems. Recent technological advances also enabled multi-omics to be achieved from the same sample or on a single analytical platform. Sample normalization is a critical step in MS-omics studies but is usually conducted independently for each omics experiment. To bridge this technical gap, we evaluated different sample normalization methods suitable for analyzing proteins, lipids, and metabolites from the same sample for multi-omics analysis. We found that normalizing samples based on tissue weight or protein concentration before or after extraction generated distinct quantitative results. Normalizing samples first by tissue weight before extraction and then by protein concentration after extraction resulted in the lowest sample variation to reveal true biological differences. We then applied this two-step normalization method to investigate multi-omics profiles of mouse brains lacking the <em>GRN</em> gene. Loss-of-function mutations in the <em>GRN</em> gene lead to the deficiency of the progranulin protein and eventually cause neurodegeneration. Comparing the proteomics, lipidomics, and metabolomics profiles of <em>GRN</em> KO and WT mouse brains revealed molecular changes and pathways related to lysosomal dysfunction and neuroinflammation. In summary, we demonstrated the importance of selecting an appropriate normalization method during multi-omics sample preparation. Our normalization method is applicable to all tissue-based multi-omics studies, ensuring reliable and accurate biomolecule quantification for biological comparisons.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 7","pages":" 1271-1279"},"PeriodicalIF":3.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an01573h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An amplification-free digital droplet assay for influenza A viral RNA based on CRISPR/Cas13a†","authors":"Jiayan Liu, Taixue An, Jingjie Peng, Qinjiang Zhu, Heyang Zhao, Zhiyu Liang, Kai Mo, Tiancai Liu and Kun Wu","doi":"10.1039/D4AN01328J","DOIUrl":"10.1039/D4AN01328J","url":null,"abstract":"<p >Most of the CRISPR-based RNA detection methods are combined with amplification to improve sensitivity, which lead to some drawbacks such as aerosol pollution, complicated operation, and amplification bias. To address the above issues, we developed a digital detection method for influenza A viral RNA based on droplet microfluidics and CRISPR/Cas13a without polymerase chain reaction. We used a microsphere coupled to a capture probe to extract and concentrate the target RNA from the samples, and then restricted the target-induced CRISPR/Cas13a cleavage event to microfluidic droplets, thus enhancing the local signal intensity and enabling single-molecule detection. With a detection limit of 10 copies per μL, influenza A viral RNA can be detected in less than 1 h. Both clinical and synthetic series samples were used to validate the assay's performance. With the help of this direct RNA diagnostic method, a variety of RNA molecules can be easily and accurately detected at the single-molecule level. This research has broad prospects in clinical applications.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1151-1157"},"PeriodicalIF":3.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Infrared spectromicroscopy of biochemistry in functional single cells","authors":"Luca Quaroni and Theodora Zlateva","doi":"10.1039/D5AN90011E","DOIUrl":"10.1039/D5AN90011E","url":null,"abstract":"<p >Correction for ‘Infrared spectromicroscopy of biochemistry in functional single cells’ by Luca Quaroni <em>et al.</em>, <em>Analyst</em>, 2011, <strong>136</strong>, 3219–3232, https://doi.org/10.1039/C1AN15060J.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 5","pages":" 1022-1022"},"PeriodicalIF":3.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d5an90011e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual proximity ligation mediated chain extension and displacement assisted signal cycles for sensitive and accurate methicillin-resistant Staphylococcus aureus (MRSA) detection†","authors":"Huali Xu, Xiangke Yang, Wen Wang and Xiaomin Yuan","doi":"10.1039/D5AN00001G","DOIUrl":"10.1039/D5AN00001G","url":null,"abstract":"<p >Infectious diseases have emerged as a significant global concern, posing a substantial burden in terms of high morbidity and mortality and presenting considerable challenges in clinical diagnosis and treatment. Therefore, it is highly desired to develop new strategies for sensitive and accurate bacteria detection to address the global epidemic of antibiotic resistance. In this study, a new technique utilizing a dual proximity ligation mediated chain extension and displacement strategy was developed for precise identification and highly sensitive detection of Methicillin-Resistant <em>Staphylococcus aureus</em> (MRSA). The antibodies recognize both protein A and PBP2a on the surface of MRSA, leading to the initiation of proximity ligation and signal amplification processes. The signal amplification procedure generated a substantial number of G-quadruplex sequences, which subsequently bind with thioflavin T (ThT) to significantly amplify its fluorescence, enabling the detection of MRSA with a low detection limit of 3.5 cfu mL<small>⁻¹</small>. In this method, dual proximity ligation assays were integrated to mediate the signal amplification process, thus endowing the method with a greatly elevated specificity in both MRSA identification and signal amplification. Due to its non-label format, high selectivity, and sensitivity, this method can serve as a practical and versatile approach for detecting different bacteria in the early stages of infectious diseases.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 7","pages":" 1310-1317"},"PeriodicalIF":3.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile fabrication of anti-fouling polymeric membrane potentiometric ion sensors based on a biocide 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one-containing self-adhesive waterborne polyurethane coating†","authors":"Ying Zhou, Rongning Liang and Wei Qin","doi":"10.1039/D4AN01583E","DOIUrl":"10.1039/D4AN01583E","url":null,"abstract":"<p >Polymeric membrane ion-selective electrodes (ISEs) are a powerful tool for ion sensing. However, their application in complicated environmental water samples is still a challenge owing to the occurrence of electrode biofouling. To address this issue, we propose a facile and effective method for enhancing the anti-biofouling properties of these sensors. A self-adhesive coating based on waterborne polyurethane and biocide 4,5-dichloro-2-<em>n</em>-octyl-4-isothiazolin-3-one is prepared. The anti-fouling potentiometric ion sensor can be obtained by simply drop-casting such a self-adhesive coating. The classical poly(vinyl chloride) membrane-based Ca<small>²⁺</small>-ISE is chosen as a model. Compared to the unmodified pristine Ca<small>²⁺</small>-ISE, the obtained anti-fouling sensor exhibits remarkable improved anti-fouling properties such as a much lower adhesion rate of bacteria (96.8% reduction after modification), higher anti-microbial rate and superior anti-algal properties. More importantly, the proposed potentiometric sensor displays excellent long-term response stability even when immersed in real seawater or a solution with high levels of algae for 30 days. It is anticipated that this simple and flexible approach could be extended to fabricate other anti-fouling electrochemical or optical membrane sensors. This work may lay an important foundation for development of anti-fouling chemical sensors for environmental water monitoring.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1103-1111"},"PeriodicalIF":3.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shifted-excitation Raman difference spectroscopy and charge-shifting detection coupled with spatially offset Raman spectroscopy for heritage science†","authors":"Alberto Lux, Claudia Conti, Alessandra Botteon, Sara Mosca and Pavel Matousek","doi":"10.1039/D4AN01280A","DOIUrl":"10.1039/D4AN01280A","url":null,"abstract":"<p > <em>In situ</em> measurements have great importance since in many scientific fields certain samples cannot be moved because of diverse reasons (excessive dimensions or weight, security, logistics <em>etc.</em>). In heritage science, this is a crucial requirement due to the high value of art objects, requiring non-invasive and <em>in situ</em> analyses. Therefore, it is important to have analytical methods capable of providing relevant information also outside laboratory environments. Such measurements face multiple challenges: for example, interference from ambient light or formation of artefacts due to undesired motions of the instruments. In Raman spectroscopy, a number of solutions have been demonstrated to mitigate these effects. For instance, Shifted Excitation Raman Difference Spectroscopy (SERDS) has proven efficient in removing the fluorescence of the sample and ambient light interference, and a charge-shifting detection approach was shown to be valuable in dealing with varying ambient light. In this study, we provide a comparison of conventional Raman spectroscopy, Shifted Excitation Raman Difference Spectroscopy (SERDS), charge-shifting detection technology and a combined SERDS and charge-shifting approach, in order to evaluate their effectiveness in mitigating fast evolving interfering backgrounds (<em>e.g.</em>, varying ambient light). Further investigations were also carried out into the potential of coupling of these methods with Spatially Offset Raman Spectroscopy (SORS) to facilitate more effective non-invasive investigations of subsurface sample components (<em>e.g.</em> paint layers). The study was carried out using samples mimicking cultural heritage materials with different degrees of complexity and in the presence of fluorescence and ambient light interference. The results are, nevertheless, applicable more generally to other areas such as forensics or biomedical fields, where both dynamic and static interferences can hinder measurements.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 6","pages":" 1140-1150"},"PeriodicalIF":3.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an01280a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ratiometric Luminescence Sensing of Bio-Macromolecules via Highly Sensitive Templated Pyrene-Based nanoGUMBOS","authors":"Rajkumar Sahoo, Prabal Pramanik, Sudhir Kumar Das, Mintu Halder","doi":"10.1039/d5an00028a","DOIUrl":"https://doi.org/10.1039/d5an00028a","url":null,"abstract":"A series of pyrene-based ionic liquids comprised of a group of uniform materials based on organic salt (GUMBOS) were synthesized by incorporating pyrene butyrate with quaternary alkyl phosphonium and ammonium-based ionic liquid via ion exchange reaction incorporating a variation in alkyl chain length. Water-suspended crystalline pyrene-assimilated nanoparticles (nanoGUMBOS) were subsequently fabricated from the prepared GUMBOS via the reprecipitation method. Different photophysical and microscopic measurements were performed to confirm the crystalline molecular level stacking of pyrene fluorophores inside the nanoparticles. The pyrene moieties within the nanoGUMBOS showed a pronounced tendency for self-association upon excitation, resulting in a novel excited state aggregation-induced emission (ESAIE). Due to the bathochromically shifted strong aggregation-induced emission, this nanoGUMBOS is further employed in the sensitive and selective ratiometric detection of biomacromolecules in aqueous and serum media. As the excessive use of some biomacromolecules (heparin (HP) and protamine (PS)) can lead to undesired side effects in living systems, hence such detection can be very crucial. In most cases, interaction with biomacromolecules like heparin facilitates the formation of excimer species in pyrene systems, but to our utter surprise we observed the opposite phenomenon of excimer disintegration here. This presents a convenient method for detecting heparin in aqueous and serum media. Furthermore, using the nanoGUMBOS-heparin complex, we can also detect protamine sulfate. A detailed study of the detection mechanism is presented in this report.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"10 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}