IF 3.6 3区化学

Analyst Pub Date : 2024-12-13 DOI: 10.1039/D4AN01038H

R. González-Vegas, O. Seksek, A. Bertho, J. Bergs, R. Hirayama, T. Inaniwa, N. Matsufuji, T. Shimokawa, Y. Prezado, I. Yousef and I. Martínez-Rovira

{"title":"Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy†","authors":"R. González-Vegas, O. Seksek, A. Bertho, J. Bergs, R. Hirayama, T. Inaniwa, N. Matsufuji, T. Shimokawa, Y. Prezado, I. Yousef and I. Martínez-Rovira","doi":"10.1039/D4AN01038H","DOIUrl":"10.1039/D4AN01038H","url":null,"abstract":"Radioresistant tumours remain complex to manage with current radiotherapy (RT) techniques. Heavy ion beams were proposed for their treatment given their advantageous radiobiological properties. However, previous studies with patients resulted in serious adverse effects in the surrounding healthy tissues. Heavy ion RT could therefore benefit from the tissue-sparing effects of minibeam radiation therapy (MBRT). To investigate the potential of this combination, here we assessed the biochemical response to neon MBRT (NeMBRT) through synchrotron-based Fourier transform infrared microspectroscopy (SR-FTIRM). Healthy (BJ) and tumour (B16-F10) cell lines were subjected to seamless (broad beam) neon RT (NeBB) and NeMBRT at HIMAC. SR-FTIRM measurements were conducted at the MIRAS beamline of ALBA Synchrotron. Principal component analysis (PCA) permitted to assess the biochemical effects after the irradiations and 24 hours post-irradiation for the different RT modalities and doses. For the healthy cells, NeMBRT resulted in the most dissimilar spectral signatures from non-irradiated cells early after irradiations, mainly due to protein conformational modifications. Nevertheless, most of the damage appeared to recover one day post-RT; conversely, protein- and nucleic acid-related IR bands were strongly affected by NeBB 24 hours after treatment, suggesting superior oxidative damage and nucleic acid degradation. Tumour cells appeared to be less sensitive to NeBB than to NeMBRT shortly after RT. Still, after one day, both NeBB and the high-dose NeMBRT regions yielded important spectral modifications, suggestive of cell death processes, protein oxidation or oxidative stress. Lipid-associated spectral changes, especially due to the NeBB and NeMBRT peak groups for the tumour cell line, were consistent with reactive oxygen species attacks.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 342-352"},"PeriodicalIF":3.6,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an01038h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproducible protein quantitation of 270 human proteins at increased depth using nanoparticle-based fractionation and multiple reaction monitoring mass spectrometry with stable isotope-labelled internal standards† 使用基于纳米颗粒的分离和多重反应监测质谱法，使用稳定同位素标记的内标，在增加的深度对270种人类蛋白质进行可重复的蛋白质定量

IF 3.6 3区化学

Analyst Pub Date : 2024-12-13 DOI: 10.1039/D4AN00967C

Claudia Gaither, Robert Popp, Aaron S. Gajadhar and Christoph H. Borchers

{"title":"Reproducible protein quantitation of 270 human proteins at increased depth using nanoparticle-based fractionation and multiple reaction monitoring mass spectrometry with stable isotope-labelled internal standards†","authors":"Claudia Gaither, Robert Popp, Aaron S. Gajadhar and Christoph H. Borchers","doi":"10.1039/D4AN00967C","DOIUrl":"10.1039/D4AN00967C","url":null,"abstract":"Here we show that when using a mix of 274 light synthetic peptide standards (NAT) as surrogates for 270 human plasma proteins, as well as stable isotope-labelled standards (SIS) as normalizers (both from MRM Proteomics Inc.) for targeted quantitative analysis by liquid chromatography multiple reaction monitoring mass spectrometry (LC/MRM-MS), the Seer Proteograph™ platform allowed for the enrichment and absolute quantitation of up to an additional 62 targets (median) compared to two standard proteomic workflows without enrichment, representing an increase of 44%. The nanoparticle-based fractionation workflow resulted in improved reproducibility compared to a traditional proteomic workflow with no fractionation (median 8.3% vs. 13.1% CV). As expected, the protein concentrations in nanoparticle coronas were higher and had more compressed dynamic range in comparison to the concentrations determined either by a 3-hour Trypsin/LysC or overnight tryptic digestion methods. As the nanoparticle-based fractionation technology gains popularity, additional steps such as establishing technique-specific protein reference ranges across plasma samples and comparisons to well-established protein quantitation methods like enzyme-linked immunosorbent assay (ELISA) and LC/MRM-MS may be explored to enable absolute quantification of plasma proteins based on nanoparticle-based fractionation data.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 353-361"},"PeriodicalIF":3.6,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an00967c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An enzyme-free immunosorbent assay of staphylococcal enterotoxin B using a three-way DNA junction amplifier with an automatic reset function 使用具有自动复位功能的三向DNA结放大器进行葡萄球菌肠毒素B的无酶免疫吸附测定

IF 4.2 3区化学

Analyst Pub Date : 2024-12-11 DOI: 10.1039/d4an01370k

Wanhao Li, Jiali Xing, Jinzhong Xi, Shaofeng Yuan, Zhilong Yu, Hang Yu, Yuliang Cheng, Yahui Guo, Yunfei Xie, Weirong Yao

引用次数: 0

Development of essential oil diffusion matrices using non-ionic surfactants-supported NADES and hydrophobic NADES† 非离子表面活性剂负载NADES和疏水性NADES制备精油扩散基质

IF 3.6 3区化学

Analyst Pub Date : 2024-12-09 DOI: 10.1039/D4AN01161A

Samir Scandar, Natali Rianika Mustafa, Claudia Zadra, Maria Carla Marcotullio and Young Hae Choi

{"title":"Development of essential oil diffusion matrices using non-ionic surfactants-supported NADES and hydrophobic NADES†","authors":"Samir Scandar, Natali Rianika Mustafa, Claudia Zadra, Maria Carla Marcotullio and Young Hae Choi","doi":"10.1039/D4AN01161A","DOIUrl":"10.1039/D4AN01161A","url":null,"abstract":"Natural deep eutectic solvents (NADES) represent a significant advance in green chemistry, offering an eco-friendly alternative to conventional organic solvents for applications in extraction, reaction media, and formulations. This study explores the application of NADES in essential oil formulations, using lavender essential oil (LEO) to investigate the solubilization and release of volatile organic compounds (VOCs). Two distinct NADES formulations were evaluated: hydrophilic NADES combined with surfactants, and hydrophobic NADES. The results show that hydrophilic NADES, when combined with non-ionic surfactants, form stable emulsions that effectively solubilize and release LEO's VOCs, overcoming the high hydrophilicity limitation of NADES. Headspace analysis and gas chromatography-mass spectrometry (GC-MS) identified 25 VOCs, with differential components analyzed through orthogonal partial least-squares discriminant analysis (OPLS-DA). VOCs were well-clustered in OPLS-DA and principal component analysis (PCA), with five clearly differentiated groups (C, N1, N3, N5, and N9). However, challenges remain, particularly in the solubilization and gradual release of VOCs. In contrast, hydrophobic NADES demonstrated a controlled release mechanism, effectively encapsulating and steadily releasing VOCs, making them advantageous for sustained fragrance delivery. The study further emphasizes the importance of the Hydrophilic–Lipophilic Balance (HLB) in NADES formulations, showing that adjusting HLB through surfactant concentrations significantly influences VOC release dynamics. In conclusion, this research highlights the potential of NADES as efficient, eco-friendly solvent systems for essential oil applications. By providing insights into optimizing NADES compositions for specific solubility and release profiles, the study identifies both challenges and opportunities, paving the way for further advancements in green chemistry.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 425-434"},"PeriodicalIF":3.6,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low background electrochemical sensor based on HCR towards acute myocardial infarction-specific miRNA detection† 基于HCR的低背景电化学传感器用于急性心肌梗死特异性miRNA检测

IF 3.6 3区化学

Analyst Pub Date : 2024-12-09 DOI: 10.1039/D4AN01065E

Yan Liu, Ziqi Liu, Tingxiu Yan, Luyao Feng, Na He, Lu Tao, Li-Ping Xu and Xueji Zhang

{"title":"Low background electrochemical sensor based on HCR towards acute myocardial infarction-specific miRNA detection†","authors":"Yan Liu, Ziqi Liu, Tingxiu Yan, Luyao Feng, Na He, Lu Tao, Li-Ping Xu and Xueji Zhang","doi":"10.1039/D4AN01065E","DOIUrl":"10.1039/D4AN01065E","url":null,"abstract":"Acute myocardial infarction (AMI) accounts for a significant proportion of global fatalities, and early detection is crucial for improving patient outcomes. However, current diagnostic methods often struggle to detect AMI in its early stages. Herein, we present an electrochemical sensor utilizing a fractal gold (FracAu) electrode and hybridization chain reaction (HCR) amplification technology to detect AMI-specific microRNAs (miRNAs). When the target sequence was added, the HCR was triggered, leading to the formation of a long-nicked DNA double helix that efficiently captured a larger quantity of positively charged RuHex molecules, resulting in significant electrochemical signal amplification. More importantly, to avoid false positive signals, exonuclease I (Exo I) was introduced to selectively cleave single-stranded DNA (ssDNA) probes. These ssDNA probes, underwent random hydrolysis from hpDNA probes, could hybridize with helper DNA1 in the absence of the target, initiating the HCR process and producing a false positive signal. The inclusion of Exo I effectively avoided false positive signals and reduced background noise. Under optimized conditions, the fabricated sensor exhibited significant sensitivity and selectivity, showing a broad linear detection range from 10 pM to 10 nM and a low limit of 0.9 fM. The fabricated electrochemical sensor also successfully detected AMI-specific miRNA in real serum samples, underscoring its diagnostic promise. By providing a reliable tool for early detection, the innovative sensor holds significant potential in combating global cardiovascular disease-related mortality rates.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 362-370"},"PeriodicalIF":3.6,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142793407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of a titanium-functionalized polymeric material rich in hydrophilic groups for phosphoproteome and glycoproteome analyses in serum† 富亲水性钛功能化高分子材料的制备及其在血清中磷蛋白组和糖蛋白组分析中的应用

IF 3.6 3区化学

Analyst Pub Date : 2024-12-07 DOI: 10.1039/D4AN01195C

Xiuqin Sheng, Jiakai Chen, Jiahui Shao, Xiaoya Zhang, Bing Wang, Chuan-Fan Ding and Yinghua Yan

{"title":"Preparation of a titanium-functionalized polymeric material rich in hydrophilic groups for phosphoproteome and glycoproteome analyses in serum†","authors":"Xiuqin Sheng, Jiakai Chen, Jiahui Shao, Xiaoya Zhang, Bing Wang, Chuan-Fan Ding and Yinghua Yan","doi":"10.1039/D4AN01195C","DOIUrl":"10.1039/D4AN01195C","url":null,"abstract":"The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and N,N-methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (Cys-PVME-Ti4+) for the enrichment of phosphopeptides and glycopeptides. Cys-PVME-Ti4+ has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g−1, recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL−1), selectivity (1 : 2000), a loading capacity of 62.5 mg g−1, recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 395-404"},"PeriodicalIF":3.6,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of concern: An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles 关注表达：一种基于镍纳米粒子装饰的金刚石纳米线的阻抗免疫传感器

IF 4.2 3区化学

Analyst Pub Date : 2024-12-06 DOI: 10.1039/d4an90085e

Palaniappan Subramanian, Anastasiia Motorina, Weng Siang Yeap, Ken Haenen, Yannick Coffinier, Vladimir Zaitsev, Joanna Niedziolka-Jonsson, Rabah Boukherroub, Sabine Szunerits

引用次数: 0

Facile and Scalable Fabrication of Molecularly Imprinted Polymer (MIP) Sensors on Poriferous Laser-Engraved Graphene Electrodes for Stress Monitoring 多孔激光雕刻石墨烯电极上分子印迹聚合物（MIP）传感器的制备及其应用

IF 4.2 3区化学

Analyst Pub Date : 2024-12-06 DOI: 10.1039/d4an01389a

Atul Sharma, Rachel Owyeung, Ayanna Thomas, Sohel Siraj, Bishal Kumar Keshari, Parikshit Sahatiya, Sameer Sonkusale

{"title":"Facile and Scalable Fabrication of Molecularly Imprinted Polymer (MIP) Sensors on Poriferous Laser-Engraved Graphene Electrodes for Stress Monitoring","authors":"Atul Sharma, Rachel Owyeung, Ayanna Thomas, Sohel Siraj, Bishal Kumar Keshari, Parikshit Sahatiya, Sameer Sonkusale","doi":"10.1039/d4an01389a","DOIUrl":"https://doi.org/10.1039/d4an01389a","url":null,"abstract":"Monitoring cortisol levels is essential for understanding the body's response to stress. Traditional cortisol testing is confined to centralized labs, and current portable platforms are based on slow and complex assays. Here, we introduce a portable, disposable, non-invasive, and sensitive electrochemical sensor strip created using electropolymerized molecularly imprinted polymers (eMIPs) on laser engraved graphene (LEG) electrodes for rapid, simple, and reliable salivary cortisol detection. Herein, the characteristics of LEGs generated on a polyimide (PI) film with different laser processing parameters are also studied and optimized. The sensor quantifies salivary cortisol by selectively binding onto the cortisol-imprinted electropolymerized polypyrrole-Prussian blue (eMIP-PPy/PB) film on LEG electrodes. The PB redox probes embedded in the eMIP produce direct electrical signals upon cortisol binding, allowing sensitive and label-free amperometric detection. The developed cort-eMIP/LEG sensor strip displays an outstanding dynamic range (0.10 to 10,000 pg mL-1), a remarkable limit of detection (0.08 pg mL-1), and a strong correlation coefficient (R2) of 0.9983 (n=4) for cortisol detection in human saliva. A rapid 3-minute analysis can more effectively measure cortisol levels in real-time than traditional methods. This sensor's performance was evaluated in human samples and validated two-way using enzyme-linked immunosorbent assays (ELISAs) and a third-party provider, Salimetrics, on 12 student volunteers exposed to varying stress levels. Results show an excellent correlation (r = 0.9948) between the developed sensors and standardized tests. The cort-eMIP/LEG cortisol sensor strip offers a simple, accessible, sample-to-answer diagnostic platform for stress monitoring.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"8 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of concern: Comparison of photo- and Cu(I)-catalyzed “click” chemistries for the formation of carbohydrate SPR interfaces 关注表达：比较光和Cu(I)催化的碳水化合物SPR界面形成的“点击”化学反应

IF 4.2 3区化学

Analyst Pub Date : 2024-12-05 DOI: 10.1039/d4an90090a

Nazek Maalouli, Alexandre Barras, Aloysius Siriwardena, Mohamed Bouazaoui, Rabah Boukherroub, Sabine Szunerits

引用次数: 0

Assessing endothelial cytotoxicity induced by tyrosine kinase inhibitors: insights from Raman and fluorescence imaging† 评估酪氨酸激酶抑制剂诱导的内皮细胞毒性：来自拉曼和荧光成像的见解

IF 3.6 3区化学

Analyst Pub Date : 2024-12-05 DOI: 10.1039/D4AN01154F

Jagoda Orleanska, Weronika Krol and Katarzyna Majzner

{"title":"Assessing endothelial cytotoxicity induced by tyrosine kinase inhibitors: insights from Raman and fluorescence imaging†","authors":"Jagoda Orleanska, Weronika Krol and Katarzyna Majzner","doi":"10.1039/D4AN01154F","DOIUrl":"10.1039/D4AN01154F","url":null,"abstract":"Since their approval, tyrosine kinase inhibitors (TKIs) have been widely used in antitumor therapy for chronic myeloblastic leukemia. Despite being approved by the FDA in 2001 to treat a rare cancer called chronic myeloid leukemia (CML), imatinib and other TKIs remain subjects of research for several reasons, such as their long-term effects, resistance, or molecular mechanisms. This study uses Raman and fluorescence imaging to investigate the in vitro cytotoxic effects of two TKIs, imatinib and dasatinib, on human aortic endothelial cells (HAECs). A comprehensive range of concentrations for these TKIs was applied to assess their cytotoxic impact based on viability, inflammation, and biochemical profile. Detailed data analysis revealed alterations in the biochemical profiles of cellular components, even though the viability of HAECs was around 80–90%. These changes indicate that, despite the cells retaining viability, they are experiencing considerable sub-lethal stress. Specifically, cells exposed to clinically relevant TKI concentrations showed increased signals from proteins and saturated lipids alongside decreased signals from nucleic acids, cytochromes, and unsaturated lipids. The subcellular analysis highlighted prominent changes in the perinuclear area, dominated by the endoplasmic reticulum and the cytoplasm. These findings suggest that TKIs are cytotoxic to vascular endothelium at concentrations close to those that are clinically observed. The predominant mechanism appears to involve oxidative stress-mediated inflammation, as evidenced by increased lipid content in treated cells and ICAM-1 staining. This cytotoxicity may contribute to the cardiotoxic effects observed during TKI therapy.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 3","pages":" 527-541"},"PeriodicalIF":3.6,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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