Analyst最新文献

{"title":"A novel light-harvesting ZIF-9-TCPP as a promising FRET-based ratiometric fluorescence probe for sperm mobility†","authors":"Yi-Xuan Li, Yu-Xuan Dai, Ju-Zheng Wang, Jérome Chauvin, Xue-Ji Zhang, Serge Cosnier, Robert S. Marks and Dan Shan","doi":"10.1039/D4AN01305K","DOIUrl":"10.1039/D4AN01305K","url":null,"abstract":"<p >The concentration of zinc ions in semen is significantly correlated with sperm viability and male fertility. In this work, a reliable ratiometric fluorescence probe (ZIF-9-TCPP) based on the efficient Förster resonance energy transfer (FRET) process between two luminophores, benzimidazole (BIM) and <em>meso</em>-tetra (4-carboxyphenyl) porphyrin (TCPP) for Zn<small>²⁺</small> detection has been constructed, where the emissions of BIM and TCPP are used as reference and detection signals. The proximity of BIM and TCPP in one framework (ZIF-9-TCPP) and the overlapped spectra between BIM and TCPP afford the attainment of a highly efficient FRET (around 90% efficiency). Efficient FRET improves the fluorescence intensity of porphyrin to enhance the sensitivity of detection. The unique spectral shift resulting from Zn<small>²⁺</small> binding to the porphyrin ring ensures the selectivity of detection. In addition, the response mechanism of the proposed ratiometric probes to Zn<small>²⁺</small> has been investigated. This work provides a convenient way to design an efficient FRET system and a promising method for sperm mobility detection.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 24","pages":" 5784-5790"},"PeriodicalIF":3.6,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"α-MnO2 catalysts with efficient ozone-catalyzed decomposition under high humidity conditions†","authors":"Jiafan Ji, Qianqian Yan, Yi Chen, Gaosheng Zhao, Bin Jia, Li Xu and Ping Cheng","doi":"10.1039/D4AN01095G","DOIUrl":"10.1039/D4AN01095G","url":null,"abstract":"<p >Ground-level ozone pollution poses significant risks to ecosystems and human health and requires effective control measures. This study focused on the monolithic ozone degradation catalyst based on powdered α-MnO<small>₂</small> and comprehensively investigated its catalytic performance, moisture resistance, and stability. The monolithic catalyst achieved the optimal catalytic activity with an ozone conversion rate of 99% after being calcined at 400 °C for 3 hours. The detailed characterization of the catalyst properties at pH = 1, 4, and 7 revealed the adverse effects of residual acid ions on the catalyst activity. The catalyst at pH = 7 had more oxygen vacancies, which was related to the reduction of sulfate ion residues and the exposure of more active sites during the washing process. At pH = 7 and a space velocity of 900 000 h<small>⁻¹</small>, the conversion rates of α-MnO<small>₂</small> to 18 ppm ozone reached 100% and 95% within 3 hours under 90% relative humidity and dry conditions, respectively. In addition, the monolithic catalyst exhibited significant moisture resistance and performed well in continuous alternating humidity cycle tests and sustained high humidity. It still maintained 90% ozone decomposition efficiency after 3 hours of testing under high humidity conditions. Meanwhile, the α-MnO<small>₂</small> monolithic catalyst showed excellent stability, with an ozone conversion rate exceeding 99% during the 50 – hour test period. These findings highlight the great potential of the α-MnO<small>₂</small> monolithic catalyst in ozone removal applications.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 1","pages":" 197-206"},"PeriodicalIF":3.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural determination of fructooligosaccharides and raffinose family oligosaccharides using logically derived sequence tandem mass spectrometry†","authors":"Chia Yen Liew, Wun-Long Li and Chi-Kung Ni","doi":"10.1039/D4AN00872C","DOIUrl":"10.1039/D4AN00872C","url":null,"abstract":"<p >Fructooligosaccharides (FOS) and raffinose family oligosaccharides (RFOs) are two highly abundant water-soluble carbohydrates in plants. The typical procedures for the FOS and RFO structural determination using mass spectrometry involve permethylation, followed by the hydrolysis of the permethylated oligosaccharides into monosaccharides, and then the identification of linkage positions using GC mass spectrometry. However, the determination of linkage position sequence is not straightforward, thus this method is limited to small oligosaccharides or oligosaccharides with simple linkages. In this study, we employed a new mass spectrometry method, logically derived sequence tandem mass spectrometry, to determine the structures of FOS and RFOs. We first showed that the monosaccharide and disaccharide CID spectra of aldohexose and ketohexose can be rationalized using dissociation mechanisms. Then we demonstrated that the linkage positions of FOS and RFOs can be identified, the sequence of the linkages can be determined, and the ketohexose and aldohexose in FOS and RFOs can be differentiated, suggesting this new method is useful for structural determination of FOS and RFOs.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 23","pages":" 5714-5727"},"PeriodicalIF":3.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring gunshot residue detection in fingerprints by functionalized particle-coupled matrix-assisted laser desorption/ionization mass spectrometry","authors":"Sunil S. Adav, Yan Wen Crystal Tan, Choon Teck Low, Song Wei Loo, Ridhwan Yusoff, Archana Gautam, Yuk Lin Yong, Chiew Yung Yang, Chin Chin Lim and Kee Woei Ng","doi":"10.1039/D4AN01260G","DOIUrl":"10.1039/D4AN01260G","url":null,"abstract":"<p >In firearm forensic investigations, detecting gunshot residue (GSR) is crucial for linking firearms to suspects and determining firing distance for forensic reconstruction. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) is emerging as a versatile and promising technological platform for fingerprint analysis. The capability of functionalized particles as an advanced dusting powder for visualizing latent fingerprints is widely recognized. This study aims to investigate the feasibility of employing functionalized magnetic fingerprint dusting powders for distinguishing regular and GSR fingerprints using MALDI-ToF-MS, thereby enhancing forensic evidentiary support. In this study, silica and carbon coated magnetic iron oxide particles were surface functionalized with phenyltriethoxy orthosilicate (PTEOS) or 3-aminopropyl triethoxysilane (APTES) to create hydrophobic and hydrophilic particles, respectively. Donor shooters’ fingerprints, both GSR-containing and regular, were analyzed using these functionalized particles coupled with MALDI-ToF-MS. The results demonstrated effective fingerprint visualization and conclusive discrimination between GSR-containing and regular fingerprints through orthogonal partial least squares discriminant analysis. This technique provides enhanced sensitivity, speed, and adaptability compared to conventional methods, making it a promising choice for initial detection of GSR in latent fingerprints. Moreover, when subjected to thorough analysis using advanced instruments, it has the potential to significantly strengthen the probative value of fingerprint evidence in forensic investigations.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 23","pages":" 5704-5713"},"PeriodicalIF":3.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of extracellular vesicle aggregation by single vesicle analysis†","authors":"Johan Yi, Suyeon Kim, Chungmin Han and Jaesung Park","doi":"10.1039/D4AN01112K","DOIUrl":"10.1039/D4AN01112K","url":null,"abstract":"<p >Extracellular vesicles (EVs) play pivotal roles in intercellular communication and are implicated in numerous physiological and pathological processes. Here, we introduce a quantitative technique using total internal reflection fluorescence microscopy (TIRFm)-based single vesicle analysis (SVA) to assess EV aggregation, a critical factor influencing their biological functionality. Employing two-colored fluorescent recombinant EV mixtures, this method enables precise discrimination between aggregated and non-aggregated EVs. It allows for calculating an aggregation ratio from the colocalization of fluorescence signals. We evaluate the impact of isolation methods, storage conditions, and biochemical environments on EV aggregation, including salt and pH variations and the presence of antibodies. Additionally, we quantitatively assess the efficacy of aggregation removal techniques, revealing significant variability in removal methods depending on the type of aggregates. This analytical approach is expected to enhance our understanding of EV aggregation dynamics and set a new standard for the characterization and functional analysis of EVs.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 23","pages":" 5638-5648"},"PeriodicalIF":3.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of blue light on cutaneous barrier structures and properties: NPLC/HR-MSn and Raman analyses","authors":"Léa Habib, Rime Michael-Jubeli, Marie Abboud, Roger Lteif and Ali Tfayli","doi":"10.1039/D4AN01244E","DOIUrl":"10.1039/D4AN01244E","url":null,"abstract":"<p >Skin health relies heavily on a well-maintained cutaneous barrier. While the detrimental effects of UV radiation on the epidermis are established, the impact of blue light, a significant component of sunlight and artificial sources, is less clear. This study aims to explore blue light's influence on the reconstructed human epidermis (RHE) using two complementary analytical approaches: Raman microspectroscopy and normal phase liquid chromatography coupled with high-resolution mass spectrometry (NPLC/HR-MS<small>^<em>n</em></small>). RHE samples were exposed to blue light (415 nm and 455 nm) during different stages of their maturation. Raman spectra were acquired for both irradiated and non-irradiated (control) samples. Raman descriptors were analyzed to assess potential alterations in the structural organization of proteins and lipids’ conformational changes. Additionally, lipids from RHE samples were extracted and analyzed using NPLC/HR-MS<small>^<em>n</em></small>. Blue light exposure led to changes in the structural organization of RHE lipids and proteins, as well as changes in the lipid composition. These changes varied depending on the wavelength and exposure dose. Exposure to blue light could disrupt the integrity of the skin's protective barrier, leading to increased sensitivity to environmental stressors and potential skin damage.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 23","pages":" 5693-5703"},"PeriodicalIF":3.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical sensing of sodium ions present in bovine serum using 4-tert-butylcalix[4]arene as a receptor†","authors":"Adrian Ebenezer Paul, Gaurav Gaur, Abdullah Al Souwaileh, Jerry J. Wu and Sambandam Anandan","doi":"10.1039/D4AN01297F","DOIUrl":"10.1039/D4AN01297F","url":null,"abstract":"<p >The concentration of sodium ions can cause severe imbalances (high pressure on blood vessels, swelling of the muscular tissues, and misfunctioning of the whole nervous system) to the human body when its limits are uncontrolled. Thus, it is necessary to determine the amount of sodium ions present in the blood. Electrochemical sensing is an efficient methodology for detecting lower concentrations of ions even with fewer samples. Calixarenes are one of the supramolecules with a distinctive structure that has a predominant ability to complex with alkali metals. Thus, calixarenes are widely used in the detection of sodium (Na<small>⁺</small>) ions. Herein, 4-<em>tert</em>-butylcalix[4]arene, the simplest and cheapest calixarene, is used to deduce sodium ion concentration by electrochemical sensing approach. The sensitivity of the 4-<em>tert</em>-butylcalix[4]arene/GCE was 0.35269 μA M<small>⁻¹</small> and the limit of detection was 0.1 ± 0.06 μM, the lowest range of detection reported for sodium ions through differential pulse voltammetry, which is an accurate analysis. This is because calixarenes can interact with sodium ions efficiently through the hydrophilic OH groups at the lower rim of the calixarene structure. The interaction of Na<small>⁺</small> ions with phenolic OH was confirmed by proton NMR titration against NaOH. 4-<em>tert</em>-Butylcalix[4]arene/GCE is adequately selective against various interfering metal ions, and it can also detect sodium ions with high accuracy (recovery range = 99.25–100.01%) in bovine serums.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 24","pages":" 5856-5862"},"PeriodicalIF":3.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capillary-flow driven microfluidic sensor based on tyrosinase for fast user-friendly assessment of pesticide exposures†","authors":"Claire E. Hefner, Prakash Aryal, Eric Brack, Todd Alexander and Charles S. Henry","doi":"10.1039/D4AN01203H","DOIUrl":"10.1039/D4AN01203H","url":null,"abstract":"<p >Pesticides are primarily used in agriculture to protect crops and extend their longevity. However, pesticide exposure has been linked to various acute and chronic health effects, raising significant environmental concerns. Current detection methods are often expensive and time-consuming, relying on complex instruments. Although enzyme-inhibition-based microfluidic paper-based analytical device (mPAD) platforms offer an easier alternative, they suffer from slow analyte transport and analyte adsorption issues in microchannels. Consequently, there is a need for a fast, simple, and cost-effective point-of-need platform for pesticide sensing. In this study, we present a rapid microfluidic platform for on-site pesticide residue detection. Unlike traditional mPAD platforms, our system transports pesticide samples through hollow capillary channels within seconds without adsorption of pesticides in the microchannels. While much research has focused on acetylcholinesterase inhibition on paper, this study is the first to introduce a tyrosinase inhibition-based assay on a paper platform for pesticide detection. Ziram, a representative dithiocarbamate pesticide, was detected using a colorimetric enzymatic inhibition assay. A limit of detection (LoD) of 1.5 ppm was obtained. In this study, we optimized the fast-flow device, assessed its stability and susceptibility to various interferences, and conducted real-sample tests using glove extraction to evaluate its capability in real-world settings. Spike recovery analysis revealed an extraction efficiency of 82.5% to 87.5% for leather gloves and 68.9% to 71.9% for nitrile gloves. This platform demonstrates strong selectivity against interferences, with the enzyme retaining 90% activity even after a week under the established storage protocols with room for further investigation. While primarily a proof of concept, this device shows promise as an additional tool for pesticide detection, with potential future integration into multiplexed devices.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 23","pages":" 5684-5692"},"PeriodicalIF":3.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/an/d4an01203h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved cancer diagnosis algorithm for protein mass spectrometry based on PCA and a one-dimensional neural network combining ResNet and SENet†","authors":"Liang Ma, Wenqing Gao, Xiangyang Hu, Dongdong Zhou, Chenlu Wang, Jiancheng Yu and Keqi Tang","doi":"10.1039/D4AN00784K","DOIUrl":"10.1039/D4AN00784K","url":null,"abstract":"<p >Cancer is one of the most serious health problems worldwide. Because cancer has no specific symptoms in its early stages, it is often not diagnosed until it is in advanced stages, reducing the likelihood of successful treatment. Therefore, early diagnosis of cancer is a formidable challenge. Mass spectrometry-based proteomics offers a robust technical foundation for cancer diagnosis. However, mass spectrometry data are characterized by high dimensionality, large data volume, and noise interference, which can lead to diagnostic errors in clinical applications. To address this challenge, an improved algorithm combining principal component analysis (PCA) with a convolutional neural network (CNN) algorithm (denoted as PCA-1DSE-ResCNN) was proposed to assist in analyzing high-dimensional mass spectral data. The algorithm initially reduced the dimensionality of the data through the PCA technique. Subsequently, the convolutional neural network algorithm (1DSE-ResCNN) integrating residual blocks and squeeze-and-excitation blocks was used as a classifier. This approach can not only alleviate the issues of overfitting and gradient vanishing caused by deep network layers but also reduce redundant information, enabling the algorithm to effectively learn high-dimensional data features and deal with nonlinear relationships. To validate the effectiveness of the algorithm, the high-dimensional ovarian cancer mass spectrometry dataset was selected as an example to examine its application performance in early diagnosis of ovarian cancer. The experimental results demonstrated that the PCA-1DSE-ResCNN algorithm outperforms other methods in terms of accuracy, specificity, and sensitivity on three high-dimensional ovarian cancer datasets. This study will contribute to the rapid diagnosis and early detection of cancer.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 23","pages":" 5675-5683"},"PeriodicalIF":3.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenoxy-1,2-dioxetane-based activatable chemiluminescent probes: tuning of photophysical properties for tracing enzymatic activities in living cells","authors":"Jagpreet Singh Sidhu, Gurjot Kaur, Atharva Rajesh Chavan, Mandeep K. Chahal and Rajeev Taliyan","doi":"10.1039/D4AN01082E","DOIUrl":"10.1039/D4AN01082E","url":null,"abstract":"<p >The use of chemiluminophores for tracing enzymatic activities in live-cell imaging has gained significant attention, making them valuable tools for diagnostic applications. Among various chemiluminophores, the phenoxy-1,2-dioxetane scaffold exhibits significant structural versatility and its activation is governed by the chemically initiated electron exchange luminescence (CIEEL) mechanism. This mechanism can be initiated by enzymatic activity, changes in pH, or other chemical stimuli. The photophysical properties of phenoxy-1,2-dioxetanes can be fine-tuned through the incorporation of different substituents on the phenolic ring and by anchoring them with specific triggers. This review discusses the variations in physicochemical properties, including emission maxima, quantum yield, aqueous solubility, and p<em>K</em><small>_a</small>, as influenced by structural modifications, thereby establishing a comprehensive structure–activity relationship. Furthermore, it categorises the probes based on different enzyme classes, such as hydrolase-sensitive probes, oxidoreductase-responsive probes, and transferase-activatable phenoxy-1,2-dioxetanes, offering a promising platform technology for the early diagnosis of diseases and disorders. The summary section highlights key opportunities and limitations associated with applying phenoxy-1,2-dioxetanes in achieving precise and effective enzyme assays.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 24","pages":" 5739-5761"},"PeriodicalIF":3.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/an/d4an01082e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}