Analyst最新文献

{"title":"A handheld biofluorometric system for acetone detection in exhaled breath condensates†","authors":"Geng Zhang, Kenta Ichikawa, Kenta Iitani, Yasuhiko Iwasaki and Kohji Mitsubayashi","doi":"10.1039/D4AN01281J","DOIUrl":"10.1039/D4AN01281J","url":null,"abstract":"<p >As a marker of human metabolism, acetone is important for lipid metabolism monitoring and early detection of diabetes. In this study, we developed a handheld biosensor for acetone based on fluorescence detection by utilizing the enzymatic reaction of secondary alcohol dehydrogenase (S-ADH) with β-nicotinamide adenine dinucleotide (NADH, <em>λ</em><small>_ex</small> = 340 nm, <em>λ</em><small>_em</small> = 490 nm). In the reaction, NADH is oxidized when acetone is reduced to 2-propanol by S-ADH, and the acetone concentration can be measured by detecting the amount of NADH consumed in this reaction. First, we constructed a compact and light-weight fluorometric NADH detection system (209 g for the sensing system and 342 g for the PC), which worked using battery power. Then, sensor characteristics were evaluated after optimization of the working conditions. The developed system was able to quantify acetone in a range of 510 nM–1 mM within 1 minute. The developed battery-operated acetone biosensor demonstrated its ability to measure the acetone concentration in the exhaled breath condensate of 10 healthy subjects at rest (23.4 ± 15.1 μM) and after 16 h of fasting (37.7 ± 14.7 μM) and it distinguished the results with significant differences (<em>p</em> = 0.011). With the advantages of handheld portability, and high levels of sensitivity and selectivity, this sensor is expected to be widely used in clinical diagnosis and wearable biochemical sensors in the future.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 3","pages":" 505-512"},"PeriodicalIF":3.6,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infrared imaging with visible light in microfluidic devices: the water absorption barrier†","authors":"Mona Suryana, Thomas Produit, Hongzhi Yang, Giovanni Birarda, Jegan Vishnuwardhana Shanmugar, Leonid Krivitsky, Anna Paterova and Gianluca Grenci","doi":"10.1039/D4AN01201A","DOIUrl":"10.1039/D4AN01201A","url":null,"abstract":"<p >Infrared spectro-microscopy is a powerful technique for analysing chemical maps of cells and tissues for biomedical and clinical applications, yet the strong water absorption in the mid-infrared region is a challenge to overcome, as it overlaps with the spectral fingerprints of biological components. Microfluidic chips offer ultimate control over the water layer thickness and are increasingly used in infrared spectro-microscopy. However, the actual impact of the water layer thickness on the instrument's performance is often left to the experimentalist's intuition and the peculiarities of specific instruments. Aiming to experimentally test the amount of absorption introduced by water with varying layer thicknesses, we fabricated a set of microfluidic devices with three controlled chamber thicknesses, each comprising a simple test pattern made of a well-known photoresist SU-8. We employed two infrared spectro-microscopy methods for measurements. The first method involves using a standard FTIR microscope with a benchtop infrared light source. The second method is a quantum infrared microscopy technique, where infrared imaging is achieved by detecting correlated photons in the visible range. We demonstrated that both methods enable the measurement of the absorption spectrum in the mid-IR region, even in the presence of up to a 30 μm thick water layer on top of a sample pattern. Additionally, the Q-IR technique offers practical advantages over synchrotron-based FTIR, such as reduced complexity, cost, and ease of operation.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 405-413"},"PeriodicalIF":3.6,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an01201a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light activated nanocomposite thin sheet for high throughput contactless biomolecular delivery into hard-to-transfect cells","authors":"Hima Harshan Padma, Donia Dominic, Kavitha Illath, Srabani Kar, Tuhin Subhra Santra","doi":"10.1039/d4an01331j","DOIUrl":"https://doi.org/10.1039/d4an01331j","url":null,"abstract":"High throughput intracellular delivery of biological macromolecules is crucial for cell engineering, gene expression, therapeutics, diagnostics, and clinical studies, but most existing techniques are either contact-based or has throughput limitations. Here, we report a light-activated, contactless, high throughput photoporation method for highly efficient and viable cell transfection of more than a million of cells within a minute. We fabricated reduced graphene oxide(rGO) nanoflakes mixed with polydimethylsiloxane (PDMS) nanocomposite thin sheet with an area of 3 cm2 and a thickness of ~600 µm. Upon infrared (980 nm) nanosecond pulse laser exposure, the rGO nanoflakes induced heat and created photothermal bubbles leading to cell membrane deformation and biomolecular delivery. Using this platform, we achieved delivery of small to large size cargo such as propidium iodide (PI) dye (668 Da), dextran (3000 Da), siRNA (20–24 bp), EGFP (6159 bp) and enzyme (465 kDa) in L929, N2a, Hela cells as well as hard-to-transfect NiH3T3 and HuH7 cells. The best results achieved for enzymes were ~97% transfection efficiency and 98% cell viability in Huh7 cells. This highly efficient cargo delivery tool is simple, easy to use, and the dimensions can be varied in accordance with user requirements. This safe and successful method may find applicability in diagnostics and cell therapy.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"19 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free miRNA fluorescent biosensors based on duplex-specific nucleases and silver nanoclusters†","authors":"Yuxin Zheng, Qian Wang, Zhiying Jin, Tingting Zhang, Jianshe Huang, Jianshan Ye and Xiurong Yang","doi":"10.1039/D4AN01407C","DOIUrl":"10.1039/D4AN01407C","url":null,"abstract":"<p >MicroRNAs (miRNAs) are considered reliable biomarkers for a variety of diseases. However, their low abundance in organisms and high sequence similarity of homologous miRNAs make their accurate detection challenging. Here, we constructed a novel fluorescent biosensor for the detection of miRNA-155, a potential biomarker of neuroinflammation, based on duplex-specific nuclease (DSN) assisted amplification and DNA-templated silver nanoclusters (DNA-AgNCs) as fluorescence signal probes. DSN-assisted amplification can transform unstable miRNA into stable DNA and amplify the miRNA signal at the same time. Using DNA-AgNCs as fluorescence signal probes for biosensors can avoid complex labeling processes and reduce costs. The biosensor shows excellent selectivity, reproducibility, a wide linear range (1–600 nM) with a detection limit of 0.86 nM, and potentiality for real sample detection. This work provides a potential universal biosensing platform for miRNA detection.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 3","pages":" 481-488"},"PeriodicalIF":3.6,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proximity hybridization-triggered cascade amplification for label-free SERS detection of Alzheimer's amyloid-β oligomers†","authors":"Qisheng Luo, Xin Kang, Chunyuan Zhang, He Zhang, Yongning Huang, Qianli Tang, Xianjiu Liao, Fenglei Gao and Zhao Liu","doi":"10.1039/D4AN01402B","DOIUrl":"10.1039/D4AN01402B","url":null,"abstract":"<p >Most of the existing SERS systems failed to achieve satisfactory results in early diagnosis of Alzheimer's disease owing to a lack of effective signal transduction. Herein, we developed a dual signal amplification strategy for SERS detection of amyloid-β oligomers based on proximity hybridization-triggered catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR). In the presence of the target protein and two DNA-labeled antibodies, a proximate complex formed in a homogeneous solution. Each of the AβO-DNA complexes served as a catalyst to trigger and accelerate numerous hybridization processes between MB1 and MB2. Subsequently, the single-strand fragment on the electrode surface initiated HCR, resulting in the hybridization reaction to form double-strand DNA concatemers on the substrate surface. The surface became negatively charged and allowed the absorption of silver ions on the DNA skeleton. After chemical reduction by hydroquinone, the formed silver nanoparticles could be further grown with a silver enhancement step to amplify the detectable SERS signal by absorbing rhodamine 6G as a SERS reporter on the silver nanoparticle surface. This biosensing platform had potential applications in molecular diagnostics of AD serum samples.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 264-271"},"PeriodicalIF":3.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"150 years of Analyst encompassing Raman and SERS development","authors":"Karen Faulds, Katsumasa Fujita, Pavel Matousek and Zachary D. Schultz","doi":"10.1039/D4AN90097A","DOIUrl":"10.1039/D4AN90097A","url":null,"abstract":"<p >A graphical abstract is available for this content</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 3","pages":" 446-446"},"PeriodicalIF":3.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shedding new light on the hidden complexity of seeds: chemically selective imaging of seed coats with stimulated Raman scattering microscopy","authors":"Chun-Chin Wang, Steven Penfield and Julian Moger","doi":"10.1039/D4AN01409J","DOIUrl":"10.1039/D4AN01409J","url":null,"abstract":"<p >The seed coat plays a pivotal role in seed development and germination, acting as a protective barrier and mediating interac-tions with the external environment. Traditional histochemical techniques and analytical methods have provided valuable insights into seed coat composition and function. However, these methods often suffer from limitations such as indirect chemical signatures and lack of spatial resolution. Here, we introduce stimulated Raman scattering (SRS) microscopy as a novel analytical tool for non-destructive, label-free, high-resolution mapping of biopolymers, water and applied active ingre-dients (AIs) in intact seed coats. We demonstrate the capability of SRS microscopy to perform depth-resolved, chemically selective imaging of major seed coat biopolymers (pectin, tannin, and suberin). By comparing wild type arabidopsis thali-ana seeds with genetically modified mutants deficient in suberin and tannin, we illustrate the potential for semi-quantitative analysis of biopolymer content. Furthermore, we show that SRS microscopy can track the permeability of seed coats to wa-ter using deuterated water (D<small>₂</small>O) uptake studies. Real-time imaging reveals differences in water permeation between wild type and suberin deficient seeds, highlighting the importance of seed coat composition in regulating water uptake during germina-tion. Additionally, we extend the application of SRS microscopy to large seeds, such as brassica oleracea, utilizing <em>epi</em>-detected imaging for surface studies. Finally, using a deuterated insecticide (clothianidin-d3), we demonstrate the capability of SRS microscopy to visualize the incorporation of AIs into seed coats. Our study presents SRS microscopy as a powerful tool for characterizing seed coat composition and understanding the diffusion of low molecular weight compounds into seeds. This technique offers new opportunities for designing seeds with tailored properties for improved germination and resilience to environmental stressors.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 3","pages":" 498-504"},"PeriodicalIF":3.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an01409j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive Detection of Nonfluorescent Solutes in Small Amounts of Diluted Aqueous Solutions Through Photothermally Induced Reflectivity Modulation at Glass/Aqueous Solution Interfaces","authors":"Shu-hei Urashima, Ryoji Kusaka","doi":"10.1039/d4an01247j","DOIUrl":"https://doi.org/10.1039/d4an01247j","url":null,"abstract":"This study proposes to employ photothermal reflectance (PTR) spectroscopy, typically used to assess the thermal properties of solid materials, for detecting nonluminescent solutes in small amounts of dilute aqueous solutions. In this spectroscopy, a probe beam nonresonant and a pump beam resonant to the solution are focused on a transparent material/aqueous solution interface. Since the temperature coefficients of refractive indexes depend on the media, the heat from the pump beam absorption changes the reflectivity at the material/solution interface. A limit of detection of 75 nM was achieved for an interface of a glass and an aqueous solution of a dye. Among various photothermal spectroscopic techniques, a notable advantage of reflectance spectroscopy is its straightforward optical configuration, where neither the precisely aligned beam expander nor differential beam splitting is required, making the optical system inexpensive and reproducible. Since reflectance spectroscopy only requires a material/solution interface, it is applicable to solutions confined in micrometer-sized channels or pits, allowing us to analyze samples with tiny amounts.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"38 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Recent advances and applications to cultural heritage using ATR-FTIR spectroscopy and ATR-FTIR spectroscopic imaging","authors":"Guan-Lin Liu and Sergei G. Kazarian","doi":"10.1039/D4AN90095B","DOIUrl":"10.1039/D4AN90095B","url":null,"abstract":"<p >Correction for ‘Recent advances and applications to cultural heritage using ATR-FTIR spectroscopy and ATR-FTIR spectroscopic imaging’ by Guan-Lin Liu <em>et al.</em>, <em>Analyst</em>, 2022, <strong>147</strong>, 1777–1797, https://doi.org/10.1039/D2AN00005A.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 436-436"},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d4an90095b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemistry and Electroanalytical Approaches","authors":"Damien W. M. Arrigan, Karolien De Wael, Jeffrey E. Dick, Thiago Paixão and Yi-Lun Ying","doi":"10.1039/D4AN90096K","DOIUrl":"10.1039/D4AN90096K","url":null,"abstract":"<p >A graphical abstract is available for this content</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 2","pages":" 219-219"},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}