Analyst最新文献

{"title":"A Simple, Wedged DNA Walker Electrochemical Biosensor Enabled DNA Logic System for miRNA Diagnostics","authors":"Juan Zhang, Benting Xie, Haonan He, Shuang Li, Hejun Gao, Hongquan Fu, Yunwen Liao","doi":"10.1039/d5an00707k","DOIUrl":"https://doi.org/10.1039/d5an00707k","url":null,"abstract":"DNA logic-gated operations enhance the efficiency of analyzing multiplex nucleic acid inputs, attracting significant attention. However, fully integrating DNA logic gates with diverse computational functions and signal amplification for biomedical diagnosis remains a challenge. In this study, we introduce a simple, wedged DNA walker-based amplified electrochemical method for miRNA detection. This method serves as the fundamental unit for constructing various logic circuits, enabling multiplex miRNA analysis. During the wedged DNA walking process, target miRNA initiates strand displacement polymerization, generating numerous DNA intermediate strands (M) that function as a single-legged walker. The immobilization probe on the track includes a wedge segment complementary to the intermediate strand, preventing its dissociation. The fuel strand, modified with ferrocene (Fc), drives the target strand to undergo branch migration and move progressively along the track. The stepwise movement of the intermediate strand enables the accumulation of Fc molecules, resulting in a significantly increased current response for target miRNA detection. Furthermore, by controlling logic functions through input-triggered cascade strand displacement reactions, we successfully establish NOT, AND, OR, NAND, and NOR logic gates. This DNA logic system can be extended to multi-input modes, offering great potential in DNA computing, multiplex analysis, and clinical diagnosis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"16 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A target-triggered self-assembly QFRP strategy for in situ single molecule imaging of intracellular mRNAs","authors":"Binxiao Li, Lan Xu, Zesong Jiang, Ruolin Yang, Baohong Liu","doi":"10.1039/d5an00831j","DOIUrl":"https://doi.org/10.1039/d5an00831j","url":null,"abstract":"Precise visualization of scarce mRNA transcripts is hindered by the limited performance of conventional probes and the complexity of the intracellular milieu. Here, we present a target-triggered self-assembly-based single quantum dot (QD) fluorescence resonance energy transfer (FRET) probe system (QFRP) for high-resolution mRNA imaging in diverse living cell lines. Compared with conventional single-fluorophore probes, QFRP exhibits substantially enhanced sensitivity and quantitative accuracy, achieving a detection limit as low as 35 fM while markedly suppressing false positives through dualsignal colocalization. By leveraging QDs as photostable donors and assembled Cy5 acceptors, QFRP enables effective visualization of subtle differences in mRNA expression between cancerous and normal cells, revealing essential biological heterogeneity in complex intracellular environments. These findings demonstrate the robustness, specificity, and versatility of this single-entity RET-based nanosystem, underscoring its potential for advanced molecular imaging and precision medicine, particularly in the visualized analysis of low-abundance targets within complex microenvironments related to tumor progression.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"302 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic detection based on size-encoded microbeads and rolling circle amplification for multiplex respiratory virus nucleic acid detection","authors":"Xingmeng Chen, Yi Wang, Zhun Lin, Huiling Zhang, Longqiang Yang, Fuyan Hong, Jieye Huang, Yuntao Liu, Yuanqing Zhang and Peifeng Ke","doi":"10.1039/D5AN00554J","DOIUrl":"10.1039/D5AN00554J","url":null,"abstract":"<p >Acute respiratory tract diseases (ARDs) are predominantly caused by viral infections, with one of their hallmark characteristics being multiple viral co-infections. Such multiple viral infections not only complicate therapeutic interventions but also lead to an increase in mortality rates. Most traditional bioassays, however, are limited to identifying a single type of virus, leading to missed diagnoses in samples with multiple respiratory pathogens. Consequently, there is an urgent need for the development of a detection method that exhibits high sensitivity, allows for constant-temperature operation, is user-friendly, and is capable of simultaneously detecting multiple viral nucleic acid targets. To address this challenge, we developed a novel method for the simultaneous detection of multiple respiratory viral nucleic acids using size-encoded microbeads and rolling circle amplification. Additionally, we designed a microfluidic chip featuring distinct spacer arrays that separate the microbeads into different regions based on their size, thereby generating characteristic signals indicative of varying biomarker levels. Rolling circle amplification significantly enhances the detection sensitivity on the microbeads, achieving a detection limit as low as fmol L<small>⁻¹</small>. In representative clinical samples, both the sensitivity and specificity for distinguishing target viral RNA from other respiratory diseases reached 100%. Importantly, this strategy enables simultaneous and <em>in situ</em> sensitive analysis of four viral nucleic acids. The throughput of pathogen nucleic acid detection can be further enhanced by incorporating additional microbeads of varying sizes and implementing a corresponding microchip design. We anticipate that this strategy holds substantial promise for clinical diagnosis of multiple respiratory viral infections.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 19","pages":" 4304-4312"},"PeriodicalIF":3.3,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of cisplatin uptake amid the population of cancer cells – how to 'crack this nut' using single-cell ICP-MS?","authors":"Zuzanna Szymańska, Magdalena Matczuk, Agnieszka Zuchowska, Pawel Romanczuk, Andrei R. Timerbaev, Maciej Jarosz","doi":"10.1039/d5an00152h","DOIUrl":"https://doi.org/10.1039/d5an00152h","url":null,"abstract":"A versatile tool to unravel the uptake diversity of a front-line anticancer drug, cisplatin, within a cancer cell population is proposed herein based on single-cell inductively coupled plasma mass spectrometry. An interested researcher is provided with the tutorial to help adapt such cutting-edge methodology and know-how to reliable data processing.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"17 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Portable Gas Chromatograph Linear Ion Trap Mass Spectrometer with Dual-Stage Cryogenic Preconcentration for On-Site Analysis of Atmospheric CFC-11, CFC-12, HCFC-22, and HCFC-141b","authors":"XuJie Deng, BoWen Wang, Zhang Ni, yulin chen, Shuo Zhang, Xinxin Lu, XiaoXu Li","doi":"10.1039/d5an00727e","DOIUrl":"https://doi.org/10.1039/d5an00727e","url":null,"abstract":"Despite regulatory bans on ozone-depleting substances (ODS) such as CFC-11 and CFC-12, recent atmospheric measurements have revealed unexpected emissions, potentially originating from legacy banks or unreported production. Conventional ODS monitoring relies heavily on large, laboratory-based instruments, which are unsuitable for rapid, on-site source identification. In this study, we present a portable gas chromatograph linear ion trap mass spectrometer (GC-LIT-MS) for on-site quantification of four representative ODS: CFC-11, CFC-12, HCFC-22, and HCFC-141b. The GC-LIT-MS integrates a dual-stage thermoelectric cooling (TEC)-based preconcentration system, enabling rapid temperature modulation, efficient sample preconcentration, and robust field operation. The external first-stage cryogenic preconcentration module comprises a single TEC chip, a heat-dissipating fan, and a control module, resulting in a compact and simple structure. This module can be further integrated into unmanned platforms such as drones or autonomous surface vehicles to enable on-site sampling in otherwise inaccessible locations. Key parameters, including column type (DB-WAX), sorbent composition (Carbopack B/Carboxen 1000), and thermal conditions, were optimized to achieve complete separation and quantification within 17 minutes. The method demonstrates excellent linearity (2–50 ppb, R² &gt; 0.996), low detection limits (0.06–0.21 ppb), and good repeatability (RSD &lt; 5.5%). Recoveries ranged from 97.98% to 113.56%. This portable GC-LIT-MS offers a sensitive, efficient solution for ODS emission screening, environmental enforcement, and real-time atmospheric monitoring.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"1 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144928190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensing biothiols via thiol-chromene addition triggered fluorophore activation by intramolecular cyclization","authors":"Lijia Liu, Xiang Zhang, Minmin Zuo, Haiyang Cui, Xiaoqian Liu, Yajing Wang, Hua-Dong Xu, Zhenjiang Ding, Guodong Hu","doi":"10.1039/d5an00674k","DOIUrl":"https://doi.org/10.1039/d5an00674k","url":null,"abstract":"Biological thiols constitute a vital class of redox regulators essential for maintaining intracellular homeostasis. Herein, we engineered three innovative fluorescent probes (CCZU series) through a rational molecular design strategy combining thiol-chromene addition and intramolecular cyclization mechanisms for sensing biothiols. These probes feature tunable electron-withdrawing groups that enable selective recognition of biothiols via a cascade reaction process: initial Michael addition of thiols to the α,β-unsaturated ketone moiety triggers chromene ring-opening, followed by spontaneous intramolecular cyclization to yield highly fluorescent coumarin derivatives. Systematic probe evaluation identified CCZU-3 as an optimal candidate, demonstrating superior water solubility, rapid response time (~5 min), and remarkable fluorescence amplification (&gt;180-fold) with nanomolar sensitivity. The probe's biological utility was validated through fluorescence imaging of biothiols in live cells and zebrafish. Significantly, CCZU-3 enabled fluorescent analysis of altered thiol content associated with redox imbalance in a diabetic cell models. This work establishes a novel sensing platform offering promising applications in disease diagnosis and redox-targeted therapeutic development.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"19 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144928140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: A dual-ion-selective electrode system for real-time monitoring of dissolved ammonia","authors":"Ayman H. Kamel and Hisham S. M. Abd-Rabboh","doi":"10.1039/D5AN90058A","DOIUrl":"10.1039/D5AN90058A","url":null,"abstract":"<p >Correction for ‘A dual-ion-selective electrode system for real-time monitoring of dissolved ammonia’ by Ayman H. Kamel <em>et al.</em>, <em>Analyst</em>, 2025, https://doi.org/10.1039/d5an00647c.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 19","pages":" 4457-4457"},"PeriodicalIF":3.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d5an90058a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144928191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass spectrometry analysis revealed distinct reaction pathways of d(CpoxG) with a photoactivatable Pt(IV) anticancer prodrug","authors":"Jishuai Zhang, Ziqi Ma, Jiafan Lin, Wenbing Li, Xiaoqin Wu, Yao Zhao, Fuyi Wang, Kui Wu","doi":"10.1039/d5an00728c","DOIUrl":"https://doi.org/10.1039/d5an00728c","url":null,"abstract":"The interactions between d(Cp<small>^ox</small>G) (<small>^ox</small>G = 8-oxo-guanine), a major form of oxidative damaged motif of CpG island, and a photoactivatable anticancer Pt(IV) prodrug, <em>trans,trans,trans</em>-[Pt(N<small>₃</small>)<small>₂</small>(OH)<small>₂</small>(pyridine)<small>₂</small>] (<strong>1</strong>) was investigated by electrospray ionization mass spectrometry (ESI-MS). Surprisingly, the primary MS analysis showed the major photooxidative products being platinum-free dinucleotides, d(CpGh) (<strong>2a</strong>)/d(CpIa) (<strong>2b</strong>) (possibly a mixture of the two isomers) and d(CpDGh) (<strong>3</strong>), of which the guanine was oxidized to guanidinohydantoin (Gh) or iminoallantoin (Ia), and hydroguanidinohydantoin (DGh), respectively. Moreover, two mono-platinated adducts, {[CpGh] + <strong>1</strong>′}+ (<strong>4</strong>) and {[CpDGh] + <strong>1</strong>′}+ (<strong>5</strong>) (<strong>1</strong>′ = [Pt<small>^II</small>(N<small>₃</small>)(py)<small>₂</small>]<small>²⁺</small>), and three Pt-crosslinked dinucleotide adducts, {[CpDGh]<small>₂</small> + <strong>1</strong>″}<small>²⁺</small> (<strong>6</strong>), {[CpGh] + [CpDGh] + <strong>1</strong>″}<small>²⁺</small> (<strong>7</strong>) and {[CpGh]<small>₂</small> + <strong>1</strong>″}<small>²⁺</small> (<strong>8</strong>) (<strong>1</strong>″ = [Pt<small>^II</small>(py)<small>₂</small>]<small>²⁺</small>), were observed as main platinated adducts. Tandem mass spectrometry with collision induced dissociation (CID) demonstrated that <strong>1</strong>′ bound at Gh or DGh in <strong>4</strong> and <strong>5</strong>, while the inter-dinucleotide crosslinks by <strong>1</strong>″ between DGhs, DGh and Gh, or Ghs in <strong>6</strong>, <strong>7</strong> and <strong>8</strong> were implicated. Unexpectedly, the supposed platinated d(Cp<small>^ox</small>G) adducts were not observed, indicating that <small>^ox</small>G is preferential to be further oxidated by the reactive oxygen species released during the photodecomposition of complex <strong>1</strong> rather than to coordination with the reduced Pt(II). These results revealed a more complexity of photo-interaction of complex <strong>1 </strong>with d(Cp<small>^ox</small>G) than with d(CpG), implicating that <small>^ox</small>G-containing DNA, in particular oxidative CpG island, might play a vital role in the action of mechanism of photoactivatable Pt(IV) prodrugs, deserving further exploration.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"56 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144924238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isotopic Labelling Analysis using Single Cell Mass Spectrometry","authors":"Anh Hai Vu, Sarah Ellen O'Connor, Lorenzo Caputi","doi":"10.1039/d5an00657k","DOIUrl":"https://doi.org/10.1039/d5an00657k","url":null,"abstract":"In this proof-of-concept work, we applied single-cell mass spectrometry to track the incorporation of an isotopically labelled precursor into plant specialized metabolites. The application of stable-isotope labelling to single cell systems could provide a unique window into the dynamics of synthesis and intercellular transport of structurally complex metabolites.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"23 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144924378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unsupervised machine learning for mass spectrometry imaging data analysis with in vivo isotope labeling","authors":"Raven L. Buckman Johnson, Vy T. Tat and Young Jin Lee","doi":"10.1039/D5AN00649J","DOIUrl":"10.1039/D5AN00649J","url":null,"abstract":"<p >Mass spectrometry imaging (MSI) has emerged as a powerful tool for spatial metabolomics, but untargeted data analysis has proven to be challenging. When combined with <em>in vivo</em> isotope labeling (MSI<em>i</em>), MSI provides insights into metabolic dynamics with high spatial resolution; however, the data analysis becomes even more complex. Although various tools exist for advanced MSI analyses, machine learning (ML) applications to MSI<em>i</em> have not been explored. In this study, we leverage Cardinal to process MSI<em>i</em> datasets of duckweeds labeled with either <small>¹³</small>CO<small>₂</small> or D<small>₂</small>O. We apply spatial shrunken centroid (SSC) segmentation, an unsupervised ML algorithm, to differentiate metabolite localizations and investigate isotope labeling of untargeted metabolites. In the SSC segmentation of three-day <small>¹³</small>C-labeled duckweed dataset, five spatial segments were identified based on distinct lipid isotopologue distributions, in contrast to classification of only three tissue regions in previous manual analysis based on galactolipid isotopologues. Similarly, SSC segmentation of five-day D-labeled dataset revealed five spatial segments based on distinct metabolite and isotopologue profiles. Further, this untargeted segmentation analysis of MSI<em>i</em> dataset provided insights on tissue-specific relative flux of each metabolite by calculating the fraction of <em>de novo</em> biosynthesis in each segment. Overall, the application of unsupervised machine learning to MSI<em>i</em> datasets has proven to significantly reduce analysis time, increase throughput, and improve the clarity of spatial isotopologue distributions.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 19","pages":" 4404-4413"},"PeriodicalIF":3.3,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d5an00649j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}