IF 3.3 3区化学

Analyst Pub Date : 2025-09-10 DOI: 10.1039/d5an00534e

Sidi Tian, Yingxue Li, Jia Yao, Changxiang Huan, Wei Zhang, Shuli Li, Zhiqi Zhang, Zhen Guo, Qi Yang, Chao Li, Chuanyu Li, Jinze Li, Lianqun Zhou

{"title":"A barcode-specific immobilization interface for microfluidics-assisted uniform spatially barcoded microarray analysis.","authors":"Sidi Tian, Yingxue Li, Jia Yao, Changxiang Huan, Wei Zhang, Shuli Li, Zhiqi Zhang, Zhen Guo, Qi Yang, Chao Li, Chuanyu Li, Jinze Li, Lianqun Zhou","doi":"10.1039/d5an00534e","DOIUrl":"https://doi.org/10.1039/d5an00534e","url":null,"abstract":"Microfluidics-assisted spatially barcoded microarray technology offers a high-throughput, low-cost approach towards spatial transcriptomic profiling. A uniform barcoded microarray is crucial for spatially unbiased mRNA analysis. However, non-specific adsorption of barcoding reagents in microchannels occurs during liquid transport, causing non-uniform barcoding in the chip's functional regions. The uneven microarray further leads to biased transcriptome capture. Herein, we develop a barcode-specific immobilization (BarSI) interface with both anti-adsorption properties and biological activity for the development of uniform spatially barcoded microarray chips. We immobilize DNA probes in straight and serpentine microchannels with coefficients of variation (CV) of 2.3% and 3.2%. Based on the orthogonal barcoding system, we developed spatially barcoded microarray chips with an overall fluorescence intensity CV of 8.47 ± 1.26%, compared with the CV of 20.91 ± 2.84% of microarrays developed on conventional amino glass slides. Using the uniform spatially barcoded microarray chip, we achieved spatially unbiased detection of mouse liver mRNA with an absolute value of Moran's I below 0.05. We present an economical and accessible method for manufacturing uniform spatially barcoded microarray chips, introducing a novel strategy for unbiased transcriptome analysis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolutionizing meropenem detection: novel OVA-PtNP nanoenzymes for clinical and environmental safety. 革新美罗培南检测：用于临床和环境安全的新型OVA-PtNP纳米酶。

IF 3.3 3区化学

Analyst Pub Date : 2025-09-10 DOI: 10.1039/d5an00506j

Xiaoyun Lin, Wenli Lai, Limei Yang, Lina Wu, Yan Yuan, Xiaofen Hu, Liqing Lin

{"title":"Revolutionizing meropenem detection: novel OVA-PtNP nanoenzymes for clinical and environmental safety.","authors":"Xiaoyun Lin, Wenli Lai, Limei Yang, Lina Wu, Yan Yuan, Xiaofen Hu, Liqing Lin","doi":"10.1039/d5an00506j","DOIUrl":"https://doi.org/10.1039/d5an00506j","url":null,"abstract":"Purpose: The objective of this study is to develop a straightforward and expeditious clinical detection method for meropenem. This study aims to introduce an innovative nanoenzyme design, thereby broadening the application of platinum nanomaterials in biological detection. It seeks to facilitate the portable detection of meropenem using commercial software. Methods: Ovalbumin platinum nanoparticle units (OVA-PtNPs) were synthesized via a redox method, utilizing ovalbumin as a stabilizer for meropenem detection. The OVA-PtNPs induced a color change in TMB substrates, resulting in the formation of oxidized TMB with a strong ultraviolet absorption peak at 652 nm. Upon the addition of meropenem, the absorbance at this wavelength decreased, thereby enabling the detection of meropenem. Results: The experimental results confirmed that OVA-PtNPs were successfully prepared with a stable structure and significant oxidase-like activity, generating substantial superoxide anions (O2˙-). This method could detect meropenem in the range of 3.125-500 μg mL-1, with a detection limit of 0.75 μg mL-1. Furthermore, the meropenem concentrations in three groups of serum samples analyzed through spiked recovery experiments showed a relative standard deviation (RSD) of less than 3%. Conclusion: This research presents novel concepts for the design of nanoenzymes and the expanded application of platinum nanomaterials in biological detection.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbazole–dansyl conjugate for latent fingerprint visualization and on-site detection of date rape drugs 卡巴唑-丹酚缀合物用于约会油菜药物的潜在指纹可视化和现场检测

IF 4.2 3区化学

Analyst Pub Date : 2025-09-10 DOI: 10.1039/d5an00675a

Meghana Sharma, Shagun, Anish Chhillar, Amit Jaiswal, Abhimanew Dhir

引用次数: 0

A directional self-priming continuous-driven compartmentalized microfluidic chip for multiplexed pathogen detection 用于多路病原体检测的定向自吸连续驱动区隔微流控芯片

IF 4.2 3区化学

Analyst Pub Date : 2025-09-10 DOI: 10.1039/d5an00640f

Weibo Fang, Yating Pan, Mariam Maiga, Wenjian Cao, Ying Mu, Qiangyuan Zhu

{"title":"A directional self-priming continuous-driven compartmentalized microfluidic chip for multiplexed pathogen detection","authors":"Weibo Fang, Yating Pan, Mariam Maiga, Wenjian Cao, Ying Mu, Qiangyuan Zhu","doi":"10.1039/d5an00640f","DOIUrl":"https://doi.org/10.1039/d5an00640f","url":null,"abstract":"Rapid and efficient screening of foodborne pathogens is crucial for preventing bacterial spread and food poisoning. However, developing a multi-detection method that is easy to operate, offers good stability, and achieves high efficiency remains an enormous challenge. Existing multiplexed nucleic acid detection methods suffer from complex designs, leading to complicated operations, and non-robust sample introduction, causing primer/probe crosstalk and false-positive results. To address these issues, we developed a directional self-priming continuous-driven multiplexed detection microfluidic chip based on a snowflake shaped-inspired design with six branches, and each branch carried a unique functional input inlet for different specific targets, enabling simultaneous detection of multiple targets from one sample using monochrome fluorescence. Based on the PDMS negative pressure storage capacity, this chip explored the self-priming method that enables the continuous loading of primers/probes and samples without requiring an external power source, effectively addressing key challenges in multiplexed detection. This chip with specially designed outlet-free and symmetric inverted T-shaped microchannels allows for multi-step reagent loading and 100% compartmentalization without reagent loss, significantly reducing reagent consumption and sample loss compared to current methods. For further validation, we tested three types of foodborne pathogens (Salmonella enterica, Staphylococcus aureus, and Listeria monocytogenes) to demonstrate the chip's multiplexed detection capability, achieving a notably high sensitivity of detection as low as 10 copies per μL. Our snowflake-shaped microfluidic chip platform shows great potential for future applications in point-of-care (POC) settings, offering a robust and efficient solution for detecting foodborne pathogens.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"44 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145025827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Mobile multi-configuration clinical translational Raman system for oral cancer application 更正：移动多配置临床转化拉曼系统用于口腔癌的应用

IF 3.3 3区化学

Analyst Pub Date : 2025-09-10 DOI: 10.1039/D5AN90059J

Siddra Maryam, Sanathana Konugolu Venkata Sekar, M. Daniyal Ghauri, Edward Fahy, Marcelo Saito Nogueira, Huihui Lu, Flavien Beffara, Georges Humbert, Richeal Ni Riordain, Patrick Sheahan, Ray Burke, Kiang Wei Kho, Rekha Gautam and Stefan Andersson-Engels

引用次数: 0

Establishing a universal dry etching methodology to unveil the nanoscale crystalline structure of fiber reinforced thermoplastic composites via scanning electron microscopy 建立一种通用的干法蚀刻方法，通过扫描电子显微镜揭示纤维增强热塑性复合材料的纳米级晶体结构

IF 4.2 3区化学

Analyst Pub Date : 2025-09-09 DOI: 10.1039/d5an00789e

Yuki Yoshida, Chihiro Hamashige, Aya Takenaka, Yoshitomo Furushima, Masaru Nakada, E. Billur Sevinis Ozbulut, Takashi Yamamoto, Boris Mizaikoff

{"title":"Establishing a universal dry etching methodology to unveil the nanoscale crystalline structure of fiber reinforced thermoplastic composites via scanning electron microscopy","authors":"Yuki Yoshida, Chihiro Hamashige, Aya Takenaka, Yoshitomo Furushima, Masaru Nakada, E. Billur Sevinis Ozbulut, Takashi Yamamoto, Boris Mizaikoff","doi":"10.1039/d5an00789e","DOIUrl":"https://doi.org/10.1039/d5an00789e","url":null,"abstract":"This study aims at the establishment of a universally applicable etching methodology to unveil the nanoscale crystalline structure of the matrix resin in fiber reinforced thermoplastic (FRTP) composites via scanning electron microscopy (SEM). The crystalline structure hierarchically consists of crystalline texture, spherulite and lamella. The details of these structures are key parameters to understand the relationship with the mechanical properties of the material for the advancement. During previous studies, two novel methodologies based on optical microscopy and micro-spectroscopy were developed via an innovative polishing strategy enabling to process FRTPs at a thickness of a few micrometers. Thereby, comprehensive information on both crystalline texture and spherulite was gained. A remaining challenge was the characterization of nanometer-scale lamella, which may be accomplished via etching-SEM. However, etching pretreatments are not commonly applied, as they require detailed knowledge, expertise on selecting the appropriate etchant and conditioning to avoid structural degradation. In the present study, a simple yet powerful strategy has been established based on plasma etching with a gas mixture comprising oxygen and argon. Five types of major matrix resins (PBT, PA6, PPS, PEEK and PE) were processed, successfully resulting in the distinct and universal exposure of the lamella topography by preferentially removing amorphous polymer chains, yet, with negligible crystalline structural changes. Using the developed treatment routine, the formation of nanoscale transcrystalline structures from fiber surfaces was notably unveiled. To complement this study, the relative etching rate of the amorphous zone with respect to the lamella was determined numerically at the range of sub-nanometers per sec.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"44 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145017605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pimpinella thellungiana ameliorates liver damage in osteoporotic rats based on metabolomics and network pharmacology 基于代谢组学和网络药理学的ellungiana pinpinella改善骨质疏松大鼠肝损伤

IF 3.3 3区化学

Analyst Pub Date : 2025-09-09 DOI: 10.1039/D5AN00274E

Haixia Liu, Ying Yang, Jiayi Ma, Xinyue Lu, Yukun Zhao, Xiaole Zhang, Zhiguo Zhang and Ruyuan Zhu

{"title":"Pimpinella thellungiana ameliorates liver damage in osteoporotic rats based on metabolomics and network pharmacology","authors":"Haixia Liu, Ying Yang, Jiayi Ma, Xinyue Lu, Yukun Zhao, Xiaole Zhang, Zhiguo Zhang and Ruyuan Zhu","doi":"10.1039/D5AN00274E","DOIUrl":"10.1039/D5AN00274E","url":null,"abstract":" Background: Postmenopausal conditions can lead to metabolic disorders such as obesity and steatosis. Pimpinella thellungiana (PT), a prominent traditional Chinese medicine, exerts potential therapeutic effects against hepatic injury. Nevertheless, the extent to which PT ameliorates liver damage resulting from estrogen deficiency, along with the associated mechanisms, remains poorly understood. Purpose: This research aimed to investigate the hepatoprotective properties of PT in an ovariectomized (OVX) rat model. Methods: A bilateral ovariectomy was conducted to create an experimental model representative of liver injury induced by OVX. Serum biochemical analysis and a study of pathological changes were performed. Bone microstructure was detected using a micro-CT system. Liver metabolites were analyzed via LC-MS/MS. Network pharmacology was used to evaluate potential targets and pathways of metabolites related to PT intervention. Results: The findings indicated that the administration of PT resulted in a significant reduction in body weight, enhanced bone microarchitecture, and ameliorated liver tissue damage. Furthermore, PT treatment was able to notably modulate the levels of GGT and IL-6 while simultaneously elevating the concentrations of irisin and adiponectin and reducing the levels of insulin and leptin. Additionally, it contributed to the restoration of the metabolic profile of the liver in OVX rats. PT administration could mainly act on targets such as AKT1, BCL2 and FGF2, and associated pathways such as “insulin resistance” the “PPAR signaling pathway” and the “NF-kappa B signaling pathway”, among others. PT administration improved glycerophospholipid metabolism. Conclusions: PT mitigated liver injury caused by estrogen deficiency in OVX rats. The potential mechanism may involve the regulation of insulin resistance and the signaling pathways associated with glycerophospholipid metabolism.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 19","pages":" 4443-4456"},"PeriodicalIF":3.3,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145017601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances and applications of electrochemical mass spectrometry for real-time monitoring of electrochemical reactions 电化学质谱法实时监测电化学反应的最新进展及应用

IF 4.2 3区化学

Analyst Pub Date : 2025-09-09 DOI: 10.1039/d5an00785b

Xuemeng Zhang, Yiteng Zhang, Baozhen Yuan, Qianhao Min

{"title":"Recent advances and applications of electrochemical mass spectrometry for real-time monitoring of electrochemical reactions","authors":"Xuemeng Zhang, Yiteng Zhang, Baozhen Yuan, Qianhao Min","doi":"10.1039/d5an00785b","DOIUrl":"https://doi.org/10.1039/d5an00785b","url":null,"abstract":"Real-time monitoring of electrochemical reaction is crucial for advancing energy conversion and storage, electrocatalysis, organic electrosynthesis, and electroanalysis. Despite progress in in-situ spectroscopic and electrochemical techniques, these methods fail to directly resolve and track multiple electrogenerated species simultaneously during electrochemical processes. Electrochemical mass spectrometry (EC-MS) bridges this gap by providing direct molecular-level compositional and structural information while simultaneously monitoring the evolution of newborn species at the electrode-electrolyte interfaces (EEIs). Propelled by the ongoing improvements in ionization sources and electrochemical cells, EC-MS methods have broadened the functions scope from online detection of reaction products to rapid capture of fleeting intermediates, and most recently, to simultaneous real-time tracking of multiple intermediates dynamics. This progressive advancement establishes EC-MS as a robust methodology for mechanistic investigation of electrochemical reactions. This review focuses on the recent advances in the EC-MS methods and the applications in exploring organic electrosynthesis, electrocatalysis, lithium-ion batteries (LIBs) and electrochemiluminescence (ECL). Finally, we outline the current limitations and future directions for EC-MS technology, forecasting its expanding utility in electrochemical reaction monitoring.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"55 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145017602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A GQD-MnO2 Nanocomposite-Based Fluorescence Assay for Sensitive Detection of the Bcl-2 Gene GQD-MnO2纳米复合荧光法灵敏检测Bcl-2基因

IF 4.2 3区化学

Analyst Pub Date : 2025-09-08 DOI: 10.1039/d5an00630a

Jingwen Li, Yifei Li, Kai Zhang, Xiaomei Liu, Yuguo Tang, Kun Han

{"title":"A GQD-MnO2 Nanocomposite-Based Fluorescence Assay for Sensitive Detection of the Bcl-2 Gene","authors":"Jingwen Li, Yifei Li, Kai Zhang, Xiaomei Liu, Yuguo Tang, Kun Han","doi":"10.1039/d5an00630a","DOIUrl":"https://doi.org/10.1039/d5an00630a","url":null,"abstract":"We developed a novel fluorescence detection method utilizing graphene quantum dots (GQDs) and manganese dioxide (MnO2) nanocomposites to enhance the sensitivity and specificity of in vitro monitoring of Bcl-2 gene expression, a key factor in tumor survival and treatment resistance. This method leverages the interplay between reduced glutathione (GSH) and hairpin DNA structures (H1 and H2) to simulate a cellular environment, enabling the redox-mediated disintegration of MnO2 nanosheets and the release of GQDs, restoring fluorescence. The presence of the Bcl-2 gene triggers a catalytic hairpin assembly (CHA) reaction, amplifying the Cy3 fluorescence signal and achieving a detection limit of 20.7 pM. The system demonstrated high specificity and stability, validated through control experiments with various DNA sequences. Optimization of key parameters, including MnO2 nanosheet concentration, GSH levels, and the ratio of hairpins H1 and H2, was critical for maximizing assay performance. The MnO2 nanosheets quench GQDs fluorescence and act as carriers for DNA probes, which are released upon reduction by GSH, mimicking intracellular redox conditions. The CHA reaction further amplifies the signal, allowing sensitive detection of low target gene levels. This biosensing platform integrates the unique properties of GQDs and MnO2 nanosheets, offering a real-time, sensitive, and selective method for monitoring Bcl-2 gene expression. This approach holds significant promise for early cancer diagnosis and personalized treatment strategies.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"27 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold nanoparticles-enhanced hollow fiber surface plasmon resonance sensor for highly sensitive detection of mercury(ii) ions 金纳米粒子增强中空纤维表面等离子体共振传感器，用于高灵敏度检测汞（II）离子。

IF 3.3 3区化学

Analyst Pub Date : 2025-09-08 DOI: 10.1039/D5AN00780A

Yangyang Xu, Xiao-song Zhu and Yi-wei Shi

{"title":"Gold nanoparticles-enhanced hollow fiber surface plasmon resonance sensor for highly sensitive detection of mercury(ii) ions","authors":"Yangyang Xu, Xiao-song Zhu and Yi-wei Shi","doi":"10.1039/D5AN00780A","DOIUrl":"10.1039/D5AN00780A","url":null,"abstract":"Mercury(II) ions (Hg2+) are one of the most common and highly toxic heavy metal ions, which can contaminate the environment and damage the human health. Therefore, the precise detection of trace Hg2+ concentration is particularly important. Herein, gold nanoparticles-enhanced silver-coated hollow fiber (HF) surface plasmon resonance (SPR) sensor was developed for the highly sensitive detection of Hg2+ ions. Due to the inherent difficulty in directly detecting Hg2+ ions on the sensing surface, the advantages of gold nanoparticles (AuNPs) as signal amplification labels were exploited to improve the sensitivity of the sensor. In the presence of Hg2+ ions, we chemically modified the sensing silver film of the sensor with 4-mercaptopyridine (4-MPY) and 4-mercaptopyridine-functionalized gold nanoparticles (AuNPs/4-MPY). Owing to the specific coordination between Hg2+ and the nitrogen of pyridine, Hg2+ could be captured by 4-MPY to form a Hg(pyridine)2 complex. After adsorption of the Hg2+ ions, the strong electromagnetic coupling between the AuNPs and the silver film led to a wavelength shift of the SPR resonance peak. Experiments for the detection of Hg2+ ions were performed in the concentration range of 5 nM to 1 μM to investigate the performance of the present sensor. The sensor achieved a limit of detection (LOD) as low as 5 nM. Moreover, the developed fiber sensor exhibited excellent performance in terms of sensitivity and repeatability. Our results show this strategy has widespread applications in the field of on-site heavy metal detection and biomedical monitoring.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 19","pages":" 4427-4435"},"PeriodicalIF":3.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyst最新文献