Analyst最新文献

{"title":"Curcumin – A natural colorant-based colorimetric pH indicator for molecular diagnostics","authors":"Sivakumar Rajamanickam, Nahyung Lim, Seung Kyun Park, Nae Yoon Lee","doi":"10.1039/d4an01570c","DOIUrl":"https://doi.org/10.1039/d4an01570c","url":null,"abstract":"Loop-mediated isothermal amplification (LAMP) provides highly selective and sensitive DNA amplification and generates hydrogen ions as a by-product under weakly buffered conditions, causing the solutions’ pH to decrease from the initial basic to an acidic environment. This distinctive feature allows the color of the amplified DNA solution to change readily when suitable pH indicators are employed. In this study, curcumin, a biodegradable, non-toxic, and natural colorant, was used as a pH indicator to visually identify LAMP-amplified Staphylococcus aureus (S. aureus) and Streptococcus pneumoniae (S. pneumoniae). Curcumin (10 mM) displayed a unique color difference between negative (red) and positive (yellow) samples, and the detection process was completed within 30 s, demonstrating the effectiveness of using curcumin for on-site diagnostics. Under optimum conditions, curcumin enabled S. aureus and S. pneumoniae detection as low as 10 fg/µL and 1 pg/µL, respectively, due to its unique halochromic properties. Owing to its adaptability, ease of use, and rapid visual detection, the introduced colorimetric pH-based LAMP method can be employed as a practical alternative to conventional colorimetry for infectious pathogen identification in both laboratory and field settings.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"23 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143590203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and reliable POCT blood typing based on laser identified RBC agglutination method","authors":"Bing Xu, Yange Huang, Shuqiang Min, Xianchang Wu, Tonghuan Zhan, Jiahao Liu, Fuzhou Niu, Hui Niu","doi":"10.1039/d4an01504e","DOIUrl":"https://doi.org/10.1039/d4an01504e","url":null,"abstract":"Blood typing is a critical element of medical diagnostics and health assessment. Nevertheless, prevailing point-of-care testing (POCT) assays, including the slide method, tube method, column agglutination method, and gel test method, often necessitate costly equipment such as centrifuges, cumbersome procedures, and skilled personnel for operation. And the testing time is relatively long (~30 min), which is not applicable in emergency cases/point-of-care testing. Thus, a low-cost, simple, low blood consumption and rapid blood typing method are urgently required, especially in the resource limited areas for point-of-care applications. We present a novel method of laser identified RBC agglutination reaction to achieve accurate and reliable blood typing. Specifically, the RBC agglutination reaction (a bio-signal) is firstly converted into a laser signal (a laser beam passed or not). Subsequently, a photoresistor converts the laser signal into a specific electrical signal (a high resistance or a low one). Finally, the electrical signal is converted to an optical signal through a detection circuit (if the resistance is lower than 600 Ω, resulting in the opening of an LED light; otherwise, the LED light is off). By merely interpreting the light signals, even non-professionals can precisely determine the blood types without any risk of misinterpretation. This laser-based blood typing method can effectively avoid human errors caused by naked-eye observation or environmental interference, and can provide new insights for developing accuracy and reliability hemagglutination identification methods for POC blood typing.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"86 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143589966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-trigger entropy drive circuit based on competitive hybridization for high-specific enzyme-free detection of single nucleotide polymorphisms","authors":"Sisi Bu, Fang Yang, Tuo Huang, Qianglong Tan, Siyu Yu, Shufen Xiao, Ye Hu, Wenlin Xie, Zhihua Zhou, Yulan Tian, Jian Chen","doi":"10.1039/d5an00011d","DOIUrl":"https://doi.org/10.1039/d5an00011d","url":null,"abstract":"Single nucleotide polymorphisms (SNPs) play a pivotal role in the detection of major diseases and the breeding of molecular designs. However, current SNP detection methods often rely heavily on expensive proteases, or alternatively, enzyme-free detection methods grapple with limited specificity. Addressing this issue, our study presents an enzyme-free, highly specific, simple, and efficient detection platform. First, we introduced additional base mismatches into the traditional entropy-driven circuit (EDC) reaction to establish a foundational distinction between mutant (MT) and wild-type (WT) sequences. On this basis, we introduced the concept of competitive hybridization and developed a dual-trigger EDC reaction (DEDC) platform, which responded to both wild-type target (WT) and mutant target (MT). By strategically leveraging the signals from both WT and MT, we constructed a ratiometric signal output mode, substantially enhancing the discrimination factor between WT and MT and maximizing the specificity of the detection system. Within the DEDC reaction system, the driving force solely consists of the increase in the system's entropy, with no enzymes involved throughout the entire process, thereby achieving simple and efficient specific detection of SNPs. Notably, MT, previously considered an interference in assays, is repurposed as a trigger signal, making DEDC particularly suitable for the identification of heterozygous samples with low mutational abundances. By analyzing the performance of this platform and using it for genotyping detection of soybean real genome samples, the practical application potential of the CTMSD platform was verified. The CTMSD platform based on EDC reactions has the potential to become a universal biosensing paradigm for future biochemical applications.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"22 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143590104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive Detection of Carcinogenic Chromium (VI) Species Below the WHO Limit Using a LaCeO₃/Carbon Black Screen Printed Electrode in Batch Injection Analysis","authors":"Senthurvelan Saranya, Yashly Yesudas K, R.G. Stacey Sibiya, Buvaneswari Gopal, Annamalai Senthil Kumar","doi":"10.1039/d5an00038f","DOIUrl":"https://doi.org/10.1039/d5an00038f","url":null,"abstract":"The widespread industrial use of chromium and its subsequent release into the environment as toxic and carcinogenic hexavalent chromium (Cr(VI)) species pose significant risks to human health and the environment. The World Health Organization (WHO) has established a limit of 50 ppb (960 nM) for Cr (VI) in water samples. Developing simple, selective, and separation-free methods for the direct detection of Cr (VI) species in the environment remains a challenging task. Herein, we present a highly crystalline lanthanum cerate/carbon black chemically modified screen-printed electrode (SPE/CB@LaCeO3) as an effective electrochemical system for the high-performance and selective electrochemical reduction of toxic Cr(VI) species in pH 2 KCl-HCl solution. The CB@LaCeO3 composite is characterized by its high-density electroactive sites and enhanced electrical conductivity, which facilitate the efficient diffusion-controlled reduction of Cr(VI) species at a low reduction potential of 0.55 V vs Ag/AgCl. The modified electrode demonstrated stability and resistance to surface fouling during continuous voltammetry analysis of high Cr(VI) concentrations. A batch-injection analysis using a three-in-one screen-printed electrode, comprising carbon-working, silver-ink reference, and CB@LaCeO3 modified carbon working electrodes, exhibited excellent concentration linearity within the ranges of 5-30 ppb and 10-35 ppm, with a low detection limit of 682 ppt (signal-to-noise ratio, 3). This method was not interfered by dissolved oxygen or other common chemicals present in environmental and water systems. The linear range and detection limit achieved in this study surpass those reported in several previous works involving precious metal and organic molecule-based chemically modified electrodes. The analytical method was validated with t-test analysis. To demonstrate the applicability of this new system, batch injection analysis was performed on a wide range of real samples, including water (tap, ground, well, reverse osmosis), consumable products (coffee, tea and milk powders), and tannery effluent, using the standard addition method. This approach yielded accurate and sensitive detection of Cr(VI) species in the samples, with recovery values of approximately 100%.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"25 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143590242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SR-based μXRD-μXRF 2D mapping to study Mg-rich historical frescoes treated with inorganic conservation treatments","authors":"Elena Possenti, Costanza Miliani, Marine Cotte, Marco Realini, Chiara Colombo","doi":"10.1039/d4an01548g","DOIUrl":"https://doi.org/10.1039/d4an01548g","url":null,"abstract":"This paper proposes the novel application of synchrotron radiation (SR) micro X-ray diffraction (µXRD) and micro X-ray fluorescence (µXRF) mapping to explore the interaction of inorganic-mineral conservation treatments (ammonium oxalate, AmOx) with the decayed, magnesium-containing, layered carbonatic matrix of Cultural Heritage (CH) fresco paintings. The high quality of SR µXRD-µXRF datasets qualitatively and semi-quantitatively investigated and 2D localised at the microscale the complex mixture and stratigraphy of both Ca- and Mg- oxalate phases formed within an Italian fresco painting (XV century) displaying a high degree of compositional and microstructural heterogeneity. The comparison of the different phase maps and elemental maps showed how the phase composition of reaction products varies as a function of the i) Mg-rich or Ca-rich carbonatic regions of the fresco, ii) Ca2+ availability; iii) microstructure and state of conservation of the fresco. Moreover, they highlighted the crystallisation of the new phases within single layers and between contiguous ones of the fresco painting, demonstrating the synergic protective, passivating, and consolidating action of AmOx treatment across the fresco stratigraphy. Above all, this study proves the high potential of SR µXRD-µXRF mapping in conservation of stone materials and opens new analytical perspectives in heritage science and materials science for the advanced and non-destructive elemental and structural investigation of heterogeneous, layered, multiphase micrometric systems.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"29 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full fingerprint hyperspectral imaging of prostate cancer tissue microarrays within clinical timeframes using quantum cascade laser microscopy","authors":"Dougal Ferguson, Niels Kroeger-Lui, Domenic Dreisbach, Claire A Hart, Diego F Sanchez, Pedro Oliveira, Michael Brown, Noel William Clarke, Ashwin Sachdeva, Peter Gardner","doi":"10.1039/d5an00046g","DOIUrl":"https://doi.org/10.1039/d5an00046g","url":null,"abstract":"One of the major limitations for clinical applications of infrared spectroscopic imaging modalities is the acquisition time required to obtain reasonable images of tissues with high spatial resolution and good signal-to-noise ratio (SNR). The time to acquire a reasonable signal to noise spectroscopic scan of a standard microscope slide region of tissue can take many hours. As a trade-off, systems can allow for discrete wavenumber acquisitions, sacrificing potentially vital chemical bands in order to reach specific acquisition targets. Recent instrumentation developments now allow for the full fingerprint imaging of entire microscope slides in under 30 minutes, enabling rapid, high quality spectroscopic imaging of tissues within clinical timeframes without sacrificing frequency bands. Here we compare the data from a novel QCL microscope to an FTIR microscope covering multiple aspects of spectroscopic imaging of a large, clinically relevant, prostate cancer tissue cohort (N=1281). Comparisons of hyperspectral data acquisition quality in both achieved signal to noise and image contrast alongside the capacity for unsupervised and supervised modelling of tissue constituents are reported. We conclude that it is now possible to collect full fingerprint spectra and derive clinically relevant data in timeframe suitable for translation into the pathology laboratory without the need to resort to discrete frequency imaging with subsequent loss of information.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"8 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioluminescence Readout Lateral Flow Immunoassay Using Nanobody Targeting Aflatoxin B1","authors":"Shun Takahashi, Yuki Hiruta, Daniel Citterio","doi":"10.1039/d5an00030k","DOIUrl":"https://doi.org/10.1039/d5an00030k","url":null,"abstract":"Multiple signal detection methods are known for lateral flow immunoassays (LFIAs), with colorimetric approaches dominating the field. However, their limited sensitivity is a remaining challenge. Fluorescence-based signaling is regarded as a more sensitive method, but it comes at the cost of partial sacrifice of the user-friendliness of LFIAs due to the requirement of an excitation light source. In this context, bioluminescence providing an inherently high signal to noise ratio without the need of excitation light could be an attractive alternative. But only a few studies have demonstrated the application of bioluminescence signaling in LFIAs. This work aimed at the development of a simple bioluminescence-based LFIA for the detection of aflatoxin B1 (AFB1), used as a model target in a competitive LFIA format. Signal transduction was achieved by nanobody-nanoluciferase (Nluc) fusion proteins. These small-sized recombinant heavy-chain-only antibodies derived from the camelidae family directly linked with the Nluc enzyme produce high intensity glow-type bioluminescence in combination with the furimazine substrate. LFIA devices consisting of sample pad, nitrocellulose membrane and absorbent pad with AFB1-BSA conjugate deposited at the test line on the nitrocellulose membrane, achieved an LOD of 0.26 ng/mL for aqueous AFB1 solutions pre-mixed with Nanobody-Nluc and bioluminescence emission observed on an imaging system. More user-friendly LFIA devices with integrated conjugate pad and pre-deposited Nanobody-Nluc provided clear AFB1 concentration-dependent bioluminescence signals with low background and enabled readout with a standard digital camera, resulting in an LOD of 1.12 ng/mL. Finally, the LFIA strips have been applied in AFB1-spiked oats milk samples. The LOD of 4.09 ng/mL achieved in the real sample matrix is well below the maximum allowable residual concentration of AFB1 in the U.S. (20 ng/mL).","PeriodicalId":63,"journal":{"name":"Analyst","volume":"1 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell lipidomics: protocol development for reliable cellular profiling using capillary sampling†","authors":"Anastasia Kontiza, Johanna von Gerichten, Matt Spick, Emily Fraser, Catia Costa, Kyle D. G. Saunders, Anthony D. Whetton, Carla F. Newman and Melanie J. Bailey","doi":"10.1039/D5AN00037H","DOIUrl":"10.1039/D5AN00037H","url":null,"abstract":"<p >Single-cell lipidomics enables detailed analysis of the lipidomes of cells, but is challenged by small sample volumes, the risk of background interference and a lack of validation data. In this study, we explore the effect of different sampling variables on the lipid profiles of single pancreatic cancer cells, detected using liquid chromatography-mass spectrometry (LC-MS). We use automated and manual capillary sampling methods to isolate living single cells and evaluate different sampling media, capillary tips, aspiration volume, and temperature and humidity control. We demonstrate that automated and manual capillary sampling yield comparable lipid profiles when key parameters are controlled. Our findings highlight that appropriate blank correction, capillary tip type, and the control of aspiration volumes are all critical to preserving detection sensitivity. Conversely, choice of sampling medium does not affect lipidomics results. We also set out suggested best practices for these methodological variables, laying a foundation for robust, adaptable workflows in single-cell lipidomics for applications such as biomarker discovery and metabolic research.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 7","pages":" 1261-1270"},"PeriodicalIF":3.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/an/d5an00037h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable and Rapid Solid Sample Preparation System Utilizing Twin-Screw Mechanism for Diagnostic Applications","authors":"Ji Hyo Park, Yeong-Eun Yoo, Jae-Ho Jin, Da-In Kwon, Jae Sung Yoon, Kang Hyun Do, Younju Lee, Kwanoh Kim","doi":"10.1039/d4an01579g","DOIUrl":"https://doi.org/10.1039/d4an01579g","url":null,"abstract":"Solid specimens play a crucial role in diagnostic and analytical testing, yet their integration into in vitro diagnostics (IVD) is often limited by lenthy processing times and bulky sample preparation equipment. In this study, we introduce a novel twin-screw mechanical maceration system that enables rapid, continuous, and efficient solid sample preparation within a compact portable platform. By utilizing counter-rotating twin screws, the system generates high shear forces, significantly reducing processing time while maintaining high sample recovery efficiency. We validated its versatility across diverse solid sample types, demonstrating efficient bacterial elution from plant tissues and single-cell dissociation of animal tissues. Our device achieved bacterial elution from plant samples in under 1 minute, which is 30 times faster than conventional stomaching, while maintaining a significantly smaller footprint. For animal tissue samples, it dissociated 5 g to 100 mg tissue chunks into single-cell suspensions within 1 min. Furthermore, we explored scalability with a miniaturized device fabricated using 3D printing, which retained comparable performance while reducing volume requirements, expediting processing time, and enabling manual operation without an external power source. This rapid, compact, adaptable, and highly efficient twin-screw system outperforms conventional solid sample processing techniques, making it a promising innovation for a wide range of biomedical applications, from point-of-care diagnostics to tissue biopsies, food hygiene, and agricultural monitoring.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"10 110 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a High-Resolution Paper-Spray Mass Spectrometry Method using Street Drugs for the Early Detection of Emerging Drugs in the Unregulated Supply","authors":"Allie Miskulin, Bruce Wallace, Dennis Kumar Hore, Chris Gill","doi":"10.1039/d5an00086f","DOIUrl":"https://doi.org/10.1039/d5an00086f","url":null,"abstract":"Adulteration of the unregulated opioid supply has contributed to increasing number of overdose deaths in North America. Harm-reduction drug checking has emerged as a strategy to address increasing adulteration rates by providing information about sample composition to people who use drugs. While paper-spray mass spectrometry is capable of trace detection for drug checking, the presence of newly emerging substances often goes undetected if not included in the targeted analysis method. High-resolution mass spectrometry has not been widely used in drug checking efforts to date, but has advanced capabilities to allow the detection of newly emerging substances. We present a high-resolution paper-spray mass spectrometry method developed for the detection of newly emerging compounds in the street drug supply. The method was used to analyze a selection of opioid samples received at a drug checking service in Victoria, British Columbia, Canada. Using this approach, newly emerging adulterants, precursors and byproducts were identified in the local street drug supply.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"4 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}