Analyst最新文献

{"title":"Cu-curcumin nanozyme for detection of Cr(Ⅵ) through off-on strategy of peroxidase mimicking activity","authors":"Ziwei Chai, Aifang Zhou, Jingjing Huang, Lingbo Qu, Jia Ge, Lin Zhang, Zhaohui Li","doi":"10.1039/d4an01358a","DOIUrl":"https://doi.org/10.1039/d4an01358a","url":null,"abstract":"Hexavalent chromium ions (Cr(VI)) possesse inherent poisonous and propensity for bioaccumulation in the natural setting. Herein, Cu-curcumin nanozyme was synthesized via the one-pot solvothermal method. Due to the high peroxidase-like activity of Cu-curcumin nanozyme, a novel and economical colorimetric platform has been developed to Cr(VI) assay. In the presence of hydrogen peroxide (H2O2), Cu-curcumin nanozyme could catalyze the colorless 3,3',5,5'-tetramethylbenzidine (TMB) into the blue product oxTMB. 8-Hydroxyquinoline (8-HQ) could inhibit the catalysis of Cu-curcumin/TMB/H2O2 system, causing the blue color to fade. With the addition of Cr(VI), the peroxidase-like activity of Cu-curcumin nanozyme is lighted up significantly, leading to the restoration of the Cu-curcumin/TMB/H2O2 system system color. After optimization of the experimental parameters, a novel colorimetric sensor for Cr(VI) assay has been successfully developed with a low limit of detection (LOD) of 31.5 nM, affirming its potential for accurate Cr(VI) quantification in environmental specimens. In addition, paper strips integrated with smartphone platform enhances the versatility and portability of Cr(VI) assay, achieving the LOD of 0.1 μM.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"79 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of Regulable Chiral Recognition Platform Based on the Photothermal Effect of Popcorn-like Gold Nanoparticles/Bovine Serum Albumin","authors":"Yujie Wang, Haibo Chen, Peiming Liu, Wenrong Cai, Datong Wu, Junyao Li, Yong Kong","doi":"10.1039/d4an01266f","DOIUrl":"https://doi.org/10.1039/d4an01266f","url":null,"abstract":"Although the chiral recognition ability of nanomaterials is known to play a crucial role in the chiral discrimination of enantiomers, it remains challenging to precisely regulate the chiral recognition ability predictably and on demand. In this work, a regulable chiral recognition platform is designed based on the photothermal effect of popcorn-like gold nanoparticles (AuNPs)/bovine serum albumin (BSA). First, AuNPs with excellent chiral recognition ability are prepared for the discrimination of tyrosine (Tyr) enantiomers. Subsequently, AuNPs can be combined with BSA through the Au-S bond. The obtained AuNPs/BSA exhibits the opposite chiral recognition ability to AuNPs due to the inherent chirality of BSA. Interestingly, BSA can also be destroyed relying on the photothermal effect of AuNPs and the excitation of near-infrared (NIR) light. Therefore, the resultant AuNPs/BSA/NIR reversibly displays the same chiral recognition ability as AuNPs. A regulable chiral recognition platform is constructed for chiral discrimination of Tyr enantiomers, which can satisfy the purpose of predictable and on-demand regulation recognition ability of nanomaterials, which might be a potential candidate for the detection and therapy in vivo.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"14 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142742708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in glutathione fluorescent probe based small organic molecule and their bioimaging","authors":"jingdong wang, Fangjun Huo, Caixia Yin","doi":"10.1039/d4an01373e","DOIUrl":"https://doi.org/10.1039/d4an01373e","url":null,"abstract":"Glutathione (GSH), as one of the most important biological mercaptans, is involved in a variety of biological processes and is considered an important biomarker in early diagnosis and treatment as well as disease stage monitoring. Rapid and accurate detection of GSH in complex biological systems is of great significance for the analysis of pathology. Fluorescence imaging technology is widely used because of its advantages of high sensitivity, high resolution and non-destructive. In this paper, the latest research progress of GSH-responsive organic small molecule fluorescence probes in recent five years is summed up, and their response mechanisms are classified and discussed. In addition, the probe design strategy, sensing mechanism and biological application are also discussed in this review. Finally, the challenges and future research directions of developing new GSH probes are prospected. Key words: Glutathione (GSH), Small fluorescence probes, Imaging.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"7 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142718776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Length-band fluorescence-based paper analytical device for detecting dipicolinic acid via ofloxacin complexation with Cu²⁺","authors":"Nguyen Ngoc Nghia, Bui The Huy, Nguyen Huu Hieu, Nguyen Thi Kim Phuong, Yong-Ill Lee","doi":"10.1039/d4an01393j","DOIUrl":"https://doi.org/10.1039/d4an01393j","url":null,"abstract":"Dipicolinic acid (DPA) is a key biomarker of bacterial spores. In this study, we present a novel distance-based paper analytical device (d-PAD) for the fluorescence sensing of DPA. The detection mechanism relies on the complexation of ofloxacin (OFL) with Cu²⁺ ions, where Cu²⁺ quenches the fluorescence of OFL via static quenching. Upon the introduction of DPA, it interacts with the OFL-Cu²⁺ complex, resulting in an enhanced fluorescence signal from OFL. The assay demonstrated a limit of detection (LOD) of 0.08 µM over a range of 0.6–120 µM, as measured by a spectrofluorometer. The d-PAD was designed for efficient reagent transport through capillary action on paper substrates, allowing for rapid on-site DPA analysis without requiring advanced laboratory equipment. The length of the fluorescent bands on the d-PADs was proportional to the concentration of DPA, providing a simple and effective readout method. With a sensitivity of 0.6 µM, the device shows a strong response to varying DPA concentrations. This distance-based platform offers a straightforward and quantitative approach to result interpretation, making it a promising tool for detecting bacterial spores in real samples. The development and optimization of this paper-based microfluidic assay represent a significant step forward in portable diagnostic technologies.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"65 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142713219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocarbon eco-hydrogel kit: on-site visual metal ion sensing and dye cleanup, advancing the circular economy in environmental remediation†","authors":"Omkar S. Nille, Akanksha G. Kolekar, Pooja V. Devre, Sneha V. Koparde, Aniket H. Sawat, Daewon Sohn, Shashikant P. Patole, Prashant V. Anbhule, Anil H. Gore and Govind B. Kolekar","doi":"10.1039/D4AN00914B","DOIUrl":"10.1039/D4AN00914B","url":null,"abstract":"<p >The naked-eye detection of hazardous pollutants through simple and cost-effective techniques is of great interest to the scientific community and related stakeholders in analytical science. The present study emphases the development of a stimuli-responsive probe by encountering sophisticated techniques for the detection of environmental pollutants. Herein, highly swellable and fluorescent-WTR-CDs-loaded HB-Alg/Gel@WTR-CDs was fabricated through a simple extrusion dripping method. The fluorescent WTR-CDs-loaded composite hydrogel showed rapid (within 10–15 min) naked-eye detection with high selectivity and sensitivity towards Cr<small>⁶⁺</small> and Mn<small>⁷⁺</small> ions over other metal ions. The developed probe had a linear detection range of 0–10 μg mL<small>⁻¹</small> with a detection limit of 0.28 μg mL<small>⁻¹</small> and 0.30 μg mL<small>⁻¹</small> for Cr<small>⁶⁺</small> and Mn<small>⁷⁺</small> ions, respectively. Interestingly, the hydrogel-based fluorescent sensor enabled on-site naked-eye detection of real water samples with good recovery. Additionally, the recyclability and reusability approach were employed for the removal of model pollutant dyes with Alg/Gel-carbon, which was synthesized using spent hydrogel beads after sensing. The present study demonstrates the tremendous potential applications of HB-Alg/Gel@WTR-CDs for simple, low-cost and fast visual detection of environmental pollutants. According to the analytical greenness evaluation (AGREE), the developed analytical method is green with an AGREE score of 0.81 and an ecofriendly circular-economy approach.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 1","pages":" 69-80"},"PeriodicalIF":3.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142713166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-Cyclodextrin Modified Imidazole Probe Specific Recognition of Organic Acids Based on Nuclear Magnetic Resonance","authors":"Chenglin Zhang, Yanqi Du, Ye Lu, Leyi Wang, Dequan Wang, Qiang Zhang, Yong Wang, Yin Xiao","doi":"10.1039/d4an01315h","DOIUrl":"https://doi.org/10.1039/d4an01315h","url":null,"abstract":"Supramolecular interaction plays an important role in molecular recognition. Cyclodextrins (CDs) are excellent hosts that are frequently utilized in identification of chemical compounds. Proper modifications to CD can give it the ability to accurately recognize specific molecules. In this research, CD was modified with allylimidazole moiety to generate synergistic effect. Remarkably, the newly formed cation probe exhibits selective recognition of 1-adamantanecarboxylic acid from six guest molecules, which cannot be achieved by CD or allylimidazole alone. The recognition is evidenced by the retention of hydrogen-deuterium exchange (HDX) properties in formed complexes and a change in the chemical shift of protons (H-4, H-5) in the allylimidazol backbone. Wavefunction analysis was employed to demonstrate the mechanism of molecular recognition is the electrostatic interaction between host and guest molecule as well as the hydrogen bond formed by the guest molecule with the imidazole moiety H-2. This work reports an intramolecular synergistic strategy to specifically identify 1-adamantanecarboxylic acid, which could inspire probe design for various analysts.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"26 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142713222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a one-step SMN assay for genetic testing in spinal muscular atrophy via MALDI-TOF MS†","authors":"Xiaodong Xing, Xing Ji, Xinzhu Liu, Xiaohui Jin, Zhenglei He, Ajing Xu, Wengao Jiang, Wenbo Ji, Yan Liu, Jian Zhang and Xiaohui Huang","doi":"10.1039/D4AN01225A","DOIUrl":"10.1039/D4AN01225A","url":null,"abstract":"<p >Spinal muscular atrophy (SMA) is a fatal neuromuscular disorder primarily attributed to the homozygous deletion of the survival motor neuron 1 (<em>SMN1</em>) gene, with disease severity closely correlated to the copy number variations (CNV) of <em>SMN2</em>. Conventional methodologies, however, fail to provide a comprehensive gene overview of <em>SMN</em> and are often both time-intensive and costly. In this study, we present a novel one-step MALDI-TOF MS assay for SMA gene testing. To accurately quantify CNV, we incorporated <em>RPPH1</em> as an internal control alongside normal samples and competing templates targeting <em>SMN1</em>, <em>SMN2</em>, and <em>RPPH1</em> for multiple corrections. The CNV assay enables precise quantification of exons 7/8 in both <em>SMN1</em> and <em>SMN2</em> genes, achieving a kappa value of 0.935 (<em>P</em> &lt; 0.001) when compared with multiple ligation-dependent probe amplification (MLPA) during its development phase. This accuracy was further corroborated in a cohort comprising 78 individuals. To identify patients harboring compound heterozygous mutations or silent carriers, prevalent pathogenic variants along with sequence variants of <em>SMN1</em> were integrated into our analysis framework; plasmids were constructed for methodological validation purposes. Utilizing these combinatorial assays for <em>SMN</em> detection, we identified one patient exhibiting a compound heterozygous mutation characterized by genotype [0 + 1<small>^d</small>] and another subject presenting genotype [2 + 1], who harbored simultaneous variants of g.27134T &gt; G and g.27706_27707delAT. The CNV assessment combined with pathogenic variants analysis developed through MALDI-TOF MS provides a comprehensive gene profile of <em>SMN</em> within a single analytical run. Given its unparalleled cost-effectiveness and time efficiency, this approach holds significant promise for further application in clinical diagnosis as well as newborn screening for SMA.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 1","pages":" 142-153"},"PeriodicalIF":3.6,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stationary phase effects in hydrophilic interaction liquid chromatographic separation of oligonucleotides†","authors":"Scott Abernathy, Asif Rayhan and Patrick A. Limbach","doi":"10.1039/D4AN01155D","DOIUrl":"10.1039/D4AN01155D","url":null,"abstract":"<p >The use of liquid chromatography coupled with mass spectrometry (LC-MS) for the characterization of oligonucleotides and nucleic acids is a powerful analytical method. Recently, hydrophilic interaction chromatography (HILIC) has been proposed as a reasonable alternative to ion-pair reversed phase separations of oligonucleotides prior to MS. A wide variety of HILIC stationary phase surface chemistries are currently available. Although their selectivity can be considerably different, few studies have compared these chemistries for LC-MS analysis of oligonucleotides. We evaluated ten different HILIC column chemistries to understand their capabilities for separating a variety of oligonucleotides. In general, we found that most columns were ineffective at separating larger (<em>n</em> &gt; 15-mer) oligonucleotides under the mobile phase and gradient conditions evaluated here. However, several stationary phases were found to be effective for separating smaller oligonucleotides such as endonuclease digestion products. Given that early eluting oligonucleotides were found to be compatible with standard electrospray ionization conditions, several different HILIC stationary phase options are available for LC-MS studies of smaller oligonucleotides including those generated in RNA modification mapping experiments.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 1","pages":" 185-196"},"PeriodicalIF":3.6,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ monitoring of Quorum Sensing signalling molecules by a SERS chip with array micro-chambers","authors":"Lulu Liu, Wenrui Ma, Haolan Tan, Chuang Ge, Zhang Bei, Chunyan Wang, Yang Zhang, Yi Xu","doi":"10.1039/d4an00992d","DOIUrl":"https://doi.org/10.1039/d4an00992d","url":null,"abstract":"The in-situ monitoring of quorum sensing (QS) signal molecules in bacterial biofilms was crucial for the diagnosis and treatment of bacterial biofilms. In this work, a surface-enhanced Raman scattering (SERS) chip integrated with functional units of bacterial biofilm cultivation and in-situ SERS detection was proposed for the monitoring of pyocyanine, an important signal molecule and biomarker secreted by Pseudomonas aeruginosa. The SERS chip was composed of a top self-assembled Nano-silver SERS substrate layer, a PDMS layer containing array micro-chambers and a bottom quartz layer. The Nano-silver SERS substrate showed good homogeneity and stability with the enhancement factor up to 1.84×108 for pyocyanine, and the design of SERS substrate located at the top layer of chip effectively attenuated the interference of complex matrix. In merit of the SERS chip, the detection limit for pyocyanine was as low as 1nM with a wide detection range of 1 nM~100 μM. The proposed SERS chip could achieve the monitoring of pyocyanine during the growth of Pseudomonas aeruginosa biofilms with and without treatment of different types anti-bacterial drugs. Furthermore, pyocyanine secreted by Pseudomonas aeruginosa were confirmed by mass spectrometry (MS) and the concentration relationship obtained by MS/MS was consistent with the SERS detection. The proposed method based on the SERS chip for monitoring QS in bacterial biofilms had advantages of in-situ detection, flexibility and efficiency.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"17 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel ratiometric fluorescent probe based on an internal reference of lanthanide/nucleotide for alkaline phosphatase detection†","authors":"Haoran Yu, Ziqing Qiang, Yelin Sun, Mengyao Sun, Lei Zhang, Bohao Yu, Wen Lei and Weibing Zhang","doi":"10.1039/D4AN01341G","DOIUrl":"10.1039/D4AN01341G","url":null,"abstract":"<p >Based on the specific hydrolytic ability of alkaline phosphatase (ALP), a novel biocompatible ratiometric lanthanide fluorescent probe based on an internal reference (CIP@SiO<small>₂</small>-Ce/ATP-Tris) was constructed with Ce<small>³⁺</small> as the central ion, adenosine triphosphate (ATP) as the ligand, Tris as the auxiliary ligand and ciprofloxacin (CIP) encapsulated into SiO<small>₂</small> nanoparticles as the reference signal. The fluorescent probe emits characteristic fluorescence at 363 nm belonging to Ce<small>³⁺</small> as the working signal and at 435 nm belonging to CIP as the reference signal. Dephosphorylation disrupted the coordination of Ce/ATP-Tris with the enzymatic reaction of ALP, which resulted in fluorescence quenching of Ce<small>³⁺</small>. The reference fluorescence was kept stable because of the protective effect of encapsulation by SiO<small>₂</small>. The biosensor analysis method was achieved by comparing the relationship between <em>I</em><small>₄₃₅</small>/<em>I</em><small>₃₆₃</small> and ALP concentration. The detection limit is 0.0025 U L<small>⁻¹</small>, and the linear range of detection is 0.1–20 U L<small>⁻¹</small>. It was subsequently used to detect ALP in samples of fetal bovine serum and human serum, and promising results were obtained.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 1","pages":" 87-93"},"PeriodicalIF":3.6,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}