Analyst最新文献

{"title":"Expression of concern: Diamond nanowires modified with poly[3-(pyrrolyl)carboxylic acid] for the immobilization of histidine-tagged peptides","authors":"Palaniappan Subramanian, Ievgen Mazurenko, Vladimir Zaitsev, Yannick Coffinier, Rabah Boukherroub, Sabine Szunerits","doi":"10.1039/d4an90086c","DOIUrl":"https://doi.org/10.1039/d4an90086c","url":null,"abstract":"Expression of concern for ‘Diamond nanowires modified with poly[3-(pyrrolyl)carboxylic acid] for the immobilization of histidine-tagged peptides’ by Palaniappan Subramanian <em>et al.</em>, <em>Analyst</em>, 2014, <strong>139</strong>, 4343–4349, https://doi.org/10.1039/C4AN00146J.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"9 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A highly sensitive UHPLC-MS/MS method for determining 15 designer LSD analogs in biological samples with application to stability studies","authors":"Olga Wachełko, Karolina Nowak, Kaja Tusiewicz, Marcin Zawadzki, Paweł Szpot","doi":"10.1039/d4an01361a","DOIUrl":"https://doi.org/10.1039/d4an01361a","url":null,"abstract":"In recent years, the rise in the synthesis and distribution of LSD analogs in illicit drug markets, commonly referred to as “designer psychedelics”, has contributed to increased recreational use. This trend has resulted in a rising number of global reports, with law enforcement increasingly detecting these compounds in blotter papers and biological samples. In the presented paper, an UHPLC-QqQ-MS/MS method was developed for trace determination (fg mL<small>⁻¹</small>) of LSD, its designer analogs (ALD-52, AL-LAD, LAMPA, LSM-775, LSZ, MiPLA, 1B-LSD, 1cP-LSD, 1cP-MiPLA, 1P-LSD, 1P-MiPLA, 1V-LSD and 2-Bromo-LSD) and its metabolite (2-oxo-3-OH-LSD) with simultaneous separation of structural isomers. Biological samples were prepared using liquid–liquid extraction (LLE) at pH 9 (with ethyl acetate); quantification was performed in multiple reaction monitoring (MRM) mode. LSD-d<small>₃</small> was used as an internal standard. The limit of quantification (LOQ) for all substances was 0.5 pg mL<small>⁻¹</small>. Precision and accuracy did not exceed 15.8% and ±14.4%, respectively. Recovery and matrix effect values were 80.6–118.6% and ±19.4%. A stability study was conducted over 30 days under different storage conditions (25 °C, 4 °C and −20 °C) for blood, urine, plasma, and serum, collected in various test tube configurations and with different preservative agents. It was found that the collection of samples in NaF can effectively stabilize LSD analogs and minimize the conversion of <em>N</em>1-substituted compounds to LSD or MiPLA. The presented method is the most sensitive to date for analyzing designer LSD analogs in biological samples, with potential for routine clinical and forensic use, enhancing detection of emerging illicit compounds. By examining the mass spectra (QTOF-MS/MS) obtained in this study and reviewing the literature on analytical characterization of LSD analogs, we proposed fragmentation patterns to aid in future identification of new designer LSD analogs (NPS).","PeriodicalId":63,"journal":{"name":"Analyst","volume":"38 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Witnessing a Discrete Microdroplet Freezing Event via Real-Time Electrochemical Monitoring of Solution Temperature","authors":"Philip Kauffmann, Cristian Blanco-Combariza, Jeffrey E Dick","doi":"10.1039/d4an01200c","DOIUrl":"https://doi.org/10.1039/d4an01200c","url":null,"abstract":"Temperature monitoring has immediate relevance to many areas of research, from atmospheric environmental studies to biological sample and food preservation to chemical reactions. Here, we use a newly established, triple-barrel electrode to provide temperature readouts in bulk solution and microdroplets, as well as electrochemically monitor freezing events in a microdroplet. Using this method, we are able to identify distinct characteristics of a freezing aqueous droplet (supercooling, ice formation beginning and end, temperature change, and thawing) with greater temporal resolution than a standard thermocouple and without the use of microscopy. By correlating the amperometric signal change caused by alterations in the diffusion coefficient of the electrochemical system in response to temperature changes, we can calculate the instantaneous temperature at our electrode, as well as the physical behavior of ice formation and expansion. Our results suggest that these electrochemical techniques can provide real-time monitoring of the physical processes involved in aqueous temperature change and ice nucleation events. Here we present a novel method for monitoring freezing events in microenvironments using a triple-barrel, electrochemical probe. Because ice nucleation spans many research fields, it is important to have a variety of tools that can be used to better understand these frozen systems. Our data shows that electrochemistry can provide real-time information on the thermal properties of aqueous environments, and these types of measurements can be extended to microdroplets. The electrochemical signal details all of the significant moments in a droplet freezing event, from tracking the temperature drop to the supercooling event, to total crystallization of the droplet at the electrode surface, to droplet thawing. Finally, this data can be combined with Multiphysics finite element models to correlate ice growth kinetics, microdroplet viscosity, and electrochemical data. All together, these experiments show that electrochemistry can work as a stand-alone tool for monitoring freezing events with excellent temporal and spatial resolution.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"140 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantum Dot to Dye Based Fluorescent Ratiometric Immunoassay for GFAP - a biomarker for Ischaemic Stroke and Glioblastoma Multiforme","authors":"Susan Varghese, S. Madanan Anju, Merin K Abraham, Ali Ibrahim Shkhair, Geneva Indongo, Greeshma Rajeevan, B. K. Arathy, Sony George","doi":"10.1039/d4an01292e","DOIUrl":"https://doi.org/10.1039/d4an01292e","url":null,"abstract":"Ischaemic stroke and glioma; leading causes of mortality and long-term disability presents a critical challenge in healthcare, necessitating innovative approaches for early and economical diagnosis enabling effective intervention. Glial fibrillary acidic protein (GFAP), a protein produced from astrocytes; is much responsive to ischaemic stroke and glioblastoma multiforme; and its levels are correlative to the extent of brain damage. In this study, we present the development of an immunoassay probe for the fluorescence ratiometric detection of glial fibrillary acidic protein (GFAP), employing monoclonal GFAP antibody-conjugated silicon quantum dots (Ab@SiQDs) and rhodamine B dye (RhB) based immunoprobe. The probe exhibited a fluorescence emission shift from 580 nm to 530 nm in response to GFAP, demonstrating a linear detection range from 31.15 pg/mL to 243 pg/mL, with a limit of detection of 0.7 pg/mL. The immunoprobe showed high selectivity towards GFAP, discriminating it from other potential interfering biomolecules and ions. Furthermore, the probe detected GFAP in spiked serum samples, achieving a recovery rate between 83% and 111%. A low-cost paper strip assay was developed that offers significant potential for the visual detection of GFAP under ultraviolet illumination.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"19 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ICT-Based Fluorescent Nanoparticles for Selective Cyanide Ion Detection and Quantification in Apple Seeds","authors":"Upendar Reddy Gandra, Rabindranath Lo, Praveen Managutti, Abdul Mannan Butt, Pogula Sreekanth Reddy, Ahsanulhaq Qurashi, Sharmarke Mohamed, Mohamed Infas Haja Mohideen","doi":"10.1039/d4an01265h","DOIUrl":"https://doi.org/10.1039/d4an01265h","url":null,"abstract":"Cyanide is a highly toxic anion. Nonetheless, many food plants and seeds could produce endogenous cyanide, which causes great danger to human health. Thus, monitoring cyanide in food samples is critically significant. In this report, we successfully engineered a novel probe based on an acceptor-donor-acceptor (A-D-A) architecture featuring dicyanovinyl-substituted thieno[3,2-b]thiophene, termed DCVTT. The designed probe self-assembles into luminous nanoparticles (DCVTT NPs) upon introducing mixed aqueous solutions. These fluorescent nanostructures served as a ratiometric probe for detecting cyanide (CN-) ions in aqueous-based environments, owing to the robustIntramolecular Charge Transfer (ICT) characteristics of DCVTT. The A-D-A substituents in DCVTT significantly enhanced ICT behavior by promoting more efficient electron transfer between the donor and acceptor groups. This improved electron transfer process leads to heightened sensitivity in detection applications. In the case of cyanide (CN) sensing, this enhanced ICT behavior manifests as a strong colorimetric response, allowing for a visible color change before and after interaction with cyanide. Speculation regarding the interaction mechanism between DCVTT and CN- is proposed based","PeriodicalId":63,"journal":{"name":"Analyst","volume":"1 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Regression for SERS Spectrum Transformation Across Diverse Instruments","authors":"Tao Wang, Yanjun Yang, Haoran Lu, Jiaheng Cui, Xianyan Chen, Ping Ma, Wenxuan Zhong, Yiping Zhao","doi":"10.1039/d4an01177e","DOIUrl":"https://doi.org/10.1039/d4an01177e","url":null,"abstract":"Surface-enhanced Raman spectroscopy (SERS) holds remarkable potential for the rapid and portable detection of trace molecules. However, the analysis and comparison of SERS spectra are challenging due to the diverse range of instruments used for data acquisition. In this paper, a spectra instrument transformation framework based on the penalized functional regression model (SpectraFRM) is introduced for cross-instrument mapping with subsequent machine learning classification to compare transformed spectra with standard spectra. In particular, the nonparametric forms of the functional response, predictors, and coefficients employed in SepctraFRM allow for efficient modeling of the nonlinear relationship between target spectra and standard spectra. In the levae-one-out training and test of 20 analytes acorss four instruments, the results demonstrate that SpectraFRM can provide intepretable corrections to peaks and baseline spectra which lead to roughly 11% error reduction, compred with original spectra. With an additional feature extraction step, the transformed spectra outperform the original spectra by 10% in analytes identifcation tasks. Overall, the proposed method is shown to be flexible, robust, accurate, and interpretable despite varities of analytes and instruments, making it a potentially powerful tool for the standardization of SERS spectra from various instruments.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"5 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cas12a/crRNA recognition initiated self-priming mediated chain extension for colorimetric Cell-free DNA (cfDNA) analysis","authors":"Ming Li, Ting Zheng, Jiaqi Zhu, Hu Zhang, Lijuan Fan","doi":"10.1039/d4an01432d","DOIUrl":"https://doi.org/10.1039/d4an01432d","url":null,"abstract":"Cell-free DNA (cfDNA) has attracted increasing attention as a promising biomarker in liquid biopsy due to its crucial role in disease diagnosis. However, former cfDNA detection methods are commonly based on the development of target-specific primers and integrated signal amplification strategies, which may induce fault-positive results. This paper presents a sensitive yet accurate method for cfDNA detection that combines phosphorothioated-terminal hairpin creation with a self-priming extension process. This approach initiates a self-priming mediated chain extension-based signal cycle following the trans-cleavage of H0@MBs when the CRISPR-Cas12a complex os activated by target cfDNA, resulting in the production of a substantial quantity of pyrophosphate. The pyrophosphate sensing probe (pp probe) was utilized, facilitating dual high-efficiency and stable colorimetric signaling. This innovative technique for colorimetric detection of target cfDNA demonstrated exceptional sensitivity with a low limit of detection of 1.04 fM and greatly elevated selectivity, with the complete detection process taking around 60 min. In addition, this technique is capable of detecting cfDNA from the culture medium of HEK293 cells, indicating its clinical application potential. Compared with the former CRISPR-Cas system-based cfDNA method that necessitates an amplification step before detection, Cas12a was directly used to identify a target sequence that can avoid the fault target amplification. This technique is simple, accurate, and rapid, engineered to identify cancer-associated cfDNA via a highly sensitive colorimetric change, which is expected to be beneficial in applications requiring point-of-care cancer detection.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"1 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142763095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Photo-triggered Dual-gas Donor of Nitric Oxide and Hydrogen Sulfide with Fluorescence to Real-time Monitor Its Release","authors":"Afeng Hou, Zhenmei Lin, Yongfang Cheng, Yaoping Tang, Qing Chen, Lingfeng Jiang, Li Li, Ziqian Zhang","doi":"10.1039/d4an01156b","DOIUrl":"https://doi.org/10.1039/d4an01156b","url":null,"abstract":"Muti-gaseous signal molecules are significant factors to regulate various physiological and pathological processes. Therefore, studying the synergistic effects of multi-gas donors on biological systems is essential. However, different types of gas donors vary significantly in terms of gas release, including location, dose, and flux. These variations can have a dramatic impact on the biological effects of the gases and require further analysis for confirmation. Thus, a controllable dual-gas donor that enables self-reporting would be valuable for studying the biological effects of two species of gaseous signal molecules. Herin, we present a novel photo-triggered donor NHD545, which release nitric oxide and hydrogen sulfide simultaneously. Furthermore, the dual-gas release from this donor is accompanied with turn-on fluorescence, which could be utilized to monitor the NO and H2S-releasing by microscopy. With NHD545, the synerstical effect of dual-gas bioavailability is conveniently to investigate in vitro.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"4 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142760133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-channel Nano-carbon-liquid/liquid Junction Electrodes for Multi-modal Analysis: Redox-active (dopamine) and Non-redox-active (acetylcholine)","authors":"Edappalil Satheesan Anupriya, Ran Chen, Daniel Kalski, Jordyn Palmer, Mei Shen","doi":"10.1039/d4an01153h","DOIUrl":"https://doi.org/10.1039/d4an01153h","url":null,"abstract":"We present here a dual-channel nanoelectrode to detect both redox-active and non-redox-active analytes. The dual-channel nanoelectrode was developed from theta nanopipette. We developed one channel of the theta nanopipette to be a carbon nanoelectrode and the other channel to be a nano interface between two immiscible electrolyte solutions (nanoITIES) electrode, producing a nano-carbon-ITIES platform. The carbon nanoelectrode channel was developed by carbon deposition via pyrolysis followed by focused ion beam milling to measure redox-active analytes. The nanoITIES electrode channel was developed to detect non-redox-active analytes. The nano-carbon-ITIES electrodes were characterized using electrochemistry, scanning electron microscopy and transmission electron microscope. Dopamine (a redox-active analyte) and acetylcholine (a non-redox-active analyte) were measured on the dual-channel nano-carbon-ITIES platform using the carbon nanoelectrode and the nanoITIES electrode, respectively. Using cyclic voltammetry, the diffusion-limited current of dopamine and acetylcholine detection on the nano-carbon-ITIES electrode increased linearly with increasing their concentrations. Using chronoamperometry (current versus time), we showed that the nano-carbon-ITIES electrode detected acetylcholine and dopamine at the same time. The introduced first-ever dual-functional nano-carbon-ITIES electrodes expand the current literature in multi-channel electrodes for multi-purpose analysis, which is an emerging area of research. Developing the analytical capability for the simultaneous detection of acetylcholine and dopamine is a critical step towards understanding diseases and disorders where both dopamine and acetylcholine are involved.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"19 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142760132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlling chemical interface damping by removing aromatic monothiol and dithiol groups from gold nanorods using sodium borohydride solution†","authors":"Ji Min Kim and Ji Won Ha","doi":"10.1039/D4AN01187B","DOIUrl":"10.1039/D4AN01187B","url":null,"abstract":"<p >Chemical interface damping (CID) in gold nanorods (AuNRs) significantly influences their optical properties due to the direct transfer of hot electrons from the AuNRs to adsorbed molecules. Despite ongoing research on CID, reversible tuning of CID at the single particle level remains a challenging task. In this study, we investigated the adsorption and removal of thiol-functionalized aromatic molecules, specifically thiophenol (TP) and benzene-1,2-dithiol (BDT), using sodium borohydride (NaBH<small>₄</small>) solution as a reagent, with confirmation through surface-enhanced Raman scattering (SERS) measurements. We further examined the effect of NaBH<small>₄</small> solution pH, immersion time in solution, and the number of thiol groups in the adsorbate (TP and BDT) on removal efficiency from the AuNR surfaces. Additionally, we extended this approach to directly control CID in single AuNRs <em>via</em> the adsorption and desorption of TP and BDT molecules under dark-field microscopy and spectroscopy. Therefore, this study provides insights into the removal of aromatic thiol molecules using NaBH<small>₄</small>, as well as the direct control of CID in individual AuNRs.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 1","pages":" 55-59"},"PeriodicalIF":3.6,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}