IF 4.2 3区化学

Analyst Pub Date : 2025-01-27 DOI: 10.1039/d4an01505c

Kanyapat Teekayupak, Pattarachaya Preechakasedkit, Natthaya Chuaypen, Thasinas Dissayabutra, Peter A. Lieberzeit, Orawon Chailapakul, Nipapan Ruecha, Daniel Citterio

{"title":"Polymeric hydrogel integrated paper-based potentiometric ion-sensing device for the determination of sodium ions in human urine","authors":"Kanyapat Teekayupak, Pattarachaya Preechakasedkit, Natthaya Chuaypen, Thasinas Dissayabutra, Peter A. Lieberzeit, Orawon Chailapakul, Nipapan Ruecha, Daniel Citterio","doi":"10.1039/d4an01505c","DOIUrl":"https://doi.org/10.1039/d4an01505c","url":null,"abstract":"A paper-based potentiometric sensor integrated with a polymeric hydrogel has been developed for sodium ion (Na+) determination in human urine. The construction of an all-solid-state ion selective electrode (s-ISE) and an all-solid-state reference electrode (s-RE) on a photo paper substrate was achieved using an inkjet printing method. For s-ISE fabrication, carbon nanotubes (CNTs) and gold nanoparticles (AuNPs) were printed on the substrate as a nanocomposite solid contact. A polymeric hydrogel containing lithium acetate (CH3COOLi) was then prepared and used as an intermediate layer to improve the adhesion between the ion selective membrane (ISM) and the AuNP/CNT solid contact, leading to enhanced detection sensitivity. The printed s-RE consisted of a pseudo silver/silver chloride electrode (p-Ag/AgCl) coated with a polymeric hydrogel containing KCl to improve the potential stability of the sensor. Under the optimal conditions, the hydrogel-integrated paper-based potentiometric sensor provided a response toward Na+ over a linear range of 10−7 M to 1 M with a near Nernstian slope of 56.42 ± 0.68 mV per decade. This sensor exhibited fast response, good sensitivity, and reasonable selectivity for Na+ measurement. Furthermore, the developed sensor was effectively applied for the detection of Na+ in urine samples with high accuracy. The presented work can be considered as a good addition to the growing field of potentiometric analytical platforms suitable for large-scale production using inkjet printing technology.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"120 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The aggregation-caused quenching effect and fluorescence enhancement features of ZIF-90 and their feasibility for the detection and imaging of ATP in cells†

IF 3.6 3区化学

Analyst Pub Date : 2025-01-27 DOI: 10.1039/D4AN01385A

Jing-Hao Fu, Xuan Wang, Qi-He Xia, Zeng-Ping Chen, Ping-Fan Shen, Yao Chen and Ru-Qin Yu

引用次数: 0

Visualization methods for loop mediated isothermal amplification (LAMP) assays

IF 3.6 3区化学

Analyst Pub Date : 2025-01-24 DOI: 10.1039/D4AN01287A

Vinni Thekkudan Novi, Anil Kumar Meher and Abdennour Abbas

{"title":"Visualization methods for loop mediated isothermal amplification (LAMP) assays","authors":"Vinni Thekkudan Novi, Anil Kumar Meher and Abdennour Abbas","doi":"10.1039/D4AN01287A","DOIUrl":"10.1039/D4AN01287A","url":null,"abstract":"Recent advances in nucleic acid (NA) detection techniques have significantly enhanced the diagnosis of diseases caused by a range of pathogens. These NA-based methods that target specific gene sequences for identification offer high specificity. Despite the effectiveness of polymerase chain reaction (PCR), its requirement for sophisticated laboratory settings and expensive equipment restricts its accessibility, particularly in resource-limited settings. As an alternative, isothermal nucleic acid amplification methods are highly sought after due to their rapid, sensitive, and specific detection ability. Among these, loop mediated isothermal amplification (LAMP) stands out due to its simplicity, reliability, and cost-effectiveness. LAMP operates without the need for varied temperature cycles, employing a simple heating block to maintain a constant temperature, thus facilitating onsite rapid testing. In LAMP, the detection step is critical as it shows the outcome of the assay. In order to make the LAMP technique user-friendly and applicable for large scale testing, it is critical to have visual detection where the results can be observed with the naked eye. This review focuses on recent developments of LAMP visualization techniques, including the more common fluorescence, turbidity, and gel electrophoresis methods, as well as innovations in colorimetric techniques applying novel transduction methods such as nanoparticles and digital tools. Additionally, various practical applications of LAMP are discussed.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 4","pages":" 588-599"},"PeriodicalIF":3.6,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bis-pyrene polyamine receptor for fast optical detection of ketoprofen: synthesis, characterization and application in all-solid-state fluorescent sensors 用于酮洛芬快速光学检测的双芘多胺受体：合成、表征及其在全固态荧光传感器中的应用

IF 4.2 3区化学

Analyst Pub Date : 2025-01-23 DOI: 10.1039/d4an01469c

Giammarco Maria Romano, Pierangela Di Menna, Andrea Bencini, Yschtar Tecla Simonini Steiner, Massimo Innocenti, Corrado Di Natale, Roberto Paolesse, Larisa Lvova

{"title":"A bis-pyrene polyamine receptor for fast optical detection of ketoprofen: synthesis, characterization and application in all-solid-state fluorescent sensors","authors":"Giammarco Maria Romano, Pierangela Di Menna, Andrea Bencini, Yschtar Tecla Simonini Steiner, Massimo Innocenti, Corrado Di Natale, Roberto Paolesse, Larisa Lvova","doi":"10.1039/d4an01469c","DOIUrl":"https://doi.org/10.1039/d4an01469c","url":null,"abstract":"Here we report on a polyamine receptor L1 bearing pyrene fluorogenic groups for the optical assessment of non-opioid analgesic drug ketoprofen, KP. L1, composed of a diethylenetriamine moiety linked at its extremities to the 1 position of two pyrene units via methylene linkers, gives an emission at 460 nm in water/ethanol 1:1 (v:v) mixture at pH 7, likely due to the excimer formation between the two aromatic groups.. In the presence of KP a salt bridging interaction between the carboxylate group of the analyte and the central ammonium group of L1 induces a redistribution of the acidic protons in the polyamine chain causing a marked increase of the emission. This optical signal was used to detect KP in aqueous media. Inspired by this observation, the properties of all-solid-state optodes with plasticized PVC membranes doped with L1 and deposited on the appropriate solid support material were further tested. The best membrane contained 1wt% of fully protonated L1, plasticized with DOS and doped with 3 equiv. of TDMACl anion-exchanger. It was able to detect KP in 2μM–0.1mM range with a low influence of interfering ions and applied for KP assessment in OkiTask with RSD of 2.1% and recovery of 102%. Moreover, the possibility to low down the KP DL to 0.84 μM (0.21mg/L) through the application of L1 based fluorescent sensor array and chemometrics was shown.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"33 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of supplementation of macular pigment carotenoids on ocular health: a Raman spectroscopic study of human blood serum of glaucoma patients 补充黄斑色素类胡萝卜素对眼健康的影响：青光眼患者血清的拉曼光谱研究

IF 3.6 3区化学

Analyst Pub Date : 2025-01-23 DOI: 10.1039/D4AN01337A

Joy Udensi, James Loughman, Ekaterina Loskutova and Hugh J. Byrne

{"title":"Effects of supplementation of macular pigment carotenoids on ocular health: a Raman spectroscopic study of human blood serum of glaucoma patients","authors":"Joy Udensi, James Loughman, Ekaterina Loskutova and Hugh J. Byrne","doi":"10.1039/D4AN01337A","DOIUrl":"10.1039/D4AN01337A","url":null,"abstract":"Carotenoids are known for their antioxidant and vision protection roles, with dietary supplements often promoted for eye health. An initial trial, the European Nutrition in Glaucoma Management (ENIGMA), assessed macular pigment optical density (MPOD) and other ocular parameters before and after supplementing glaucoma patients with macular pigment (MP) carotenoids. The trial confirmed significant improvements in clinical ocular health. Blood, containing all major dietary carotenoids, serves as an efficient medium for in vivo analysis of carotenoids. Raman spectroscopy, an effective analytical tool, was used to measure the impact of supplementation on serum carotenoid levels and their correlation with MPOD and other ocular responses. Serum samples from baseline and 18-month supplemented participants were analysed. An inverse relationship was found between the percentage change in Raman intensity over the supplementation period and baseline Raman serum measurements, indicating greater relative benefits for people with low MPOD/serum carotenoids pre-supplementation. Partial least squares regression (PLSR) was employed to analyse the spectra after pre-processing, and the loadings reflected the carotenoid content and structural profile. MPOD results correlated at all eccentricities, with a coefficient of determination (R2) of 0.62–0.92 and %Root mean squared error of <44%. Structural, functional, and perceptual parameters also showed good correlation with serum Raman measurements. The results support the ENIGMA trial conclusions, and suggest strategies for optimizing patient responses to supplementation based on baseline carotenoid levels. Additionally, Raman spectroscopy of serum carotenoids shows significant potential as a simple and reliable method for investigating macular pigment carotenoids and assessing patient health.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 4","pages":" 630-641"},"PeriodicalIF":3.6,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A lanmodulin-based fluorescent assay for the rapid and sensitive detection of rare earth elements† 基于lanmodulin的稀土元素快速灵敏荧光检测方法

IF 3.6 3区化学

Analyst Pub Date : 2025-01-23 DOI: 10.1039/D4AN01196A

QiKe Wang, JieMiao Yu, ZhaoXiang Zhong, Cai Hui, Yi Zhang, Huizhou liu and LiangRong Yang

{"title":"A lanmodulin-based fluorescent assay for the rapid and sensitive detection of rare earth elements†","authors":"QiKe Wang, JieMiao Yu, ZhaoXiang Zhong, Cai Hui, Yi Zhang, Huizhou liu and LiangRong Yang","doi":"10.1039/D4AN01196A","DOIUrl":"10.1039/D4AN01196A","url":null,"abstract":"Sensitive and rapid detection methods for rare earth elements (REEs), including lanthanides (Lns), will facilitate the mining and recovery of these elements. Here, we innovated a rapid, highly selective and sensitive fluorescence detection method for Lns, based on Hans-Lanmodulin, a newly discovered protein with high selectivity and binding affinity for rare earth elements. By labelling the fluorescein moiety FITC onto Hans-Lanmodulin, named as FITC-Hans-LanM. When rare earth ions are present in solution, FITC-Hans-LanM will specifically bind rare earth ions undergoing a conformational change from a disordered state to a dimer, in which the FITC molecules come close to each other, resulting in decreasing fluorescence intensity or even quenching. The assay was responsive to light, medium and heavy rare earth ions. The fluorescence signal has a good linear relationship with Nd3+ concentration in the range of 1–20 nM. The detection limit of the method was 0.512 nM, within 1 min. This method could become a useful technique for the detection and quantification of rare earth elements in environmental and industrial samples.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 4","pages":" 750-759"},"PeriodicalIF":3.6,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening analysis of doping agents in horse urine and plasma with dilute and shoot using liquid chromatography high resolution mass spectrometry† 用液相色谱-高分辨质谱法对马尿液和血浆中兴奋剂进行筛选分析

IF 3.6 3区化学

Analyst Pub Date : 2025-01-23 DOI: 10.1039/D4AN01501K

Eylem Funda Göktaş, Erol Kabil, Esma Söylemez Yeşilçimen and Levent Dirikolu

{"title":"Screening analysis of doping agents in horse urine and plasma with dilute and shoot using liquid chromatography high resolution mass spectrometry†","authors":"Eylem Funda Göktaş, Erol Kabil, Esma Söylemez Yeşilçimen and Levent Dirikolu","doi":"10.1039/D4AN01501K","DOIUrl":"10.1039/D4AN01501K","url":null,"abstract":"Various technical methodologies are required to accurately detect substances of different chemical and pharmacological properties in biological samples, which are increasing in number and variety daily. Therefore, laboratories where many samples and different factors are analyzed simultaneously need methods with easy sample preparation, short analysis times and low analysis costs. In this study, the objective was to scan substances susceptible to chemical degradation, amenable to analysis without hydrolysis, and exhibiting short-term stability by employing a straightforward, expeditious, and cost-efficient method. For this purpose, a high-throughput dilute and shoot screening protocol was developed and validated utilizing high-performance liquid chromatography coupled with high-resolution mass spectrometry to analyze various pharmacological compounds in horse urine and plasma. Over 200 prohibited substances across multiple categories were scanned within a 13 minute run. Chromatographic separation was performed on a C18 column using an elution gradient of mobile phase A, 5 mM ammonium bicarbonate at pH 9, and mobile phase B, methanol, at a flow rate of 0.3 mL min−1. The method was validated according to the specifications of 2002/657/EC multi-screening requirements. The detection capability ranged from ≤1 to 200 ng mL−1 for prohibited substances. The implementation of the screening method in doping analysis, and the analysis of real positive case samples served to underscore the practical applicability of the developed method. To the best of our knowledge, this is a rare method that can be applied to both urine and plasma samples and provides a rapid, practical, broad-spectrum, and high-throughput analysis of prohibited substances in horse plasma and urine cost-effectively.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 4","pages":" 773-792"},"PeriodicalIF":3.6,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct analysis and identification of intestinal microflora of shrimp for their geographical traceability via mass spectrometry and bacterial library searching 利用质谱法和细菌文库直接分析和鉴定对虾肠道菌群的地理溯源性

IF 4.2 3区化学

Analyst Pub Date : 2025-01-22 DOI: 10.1039/d4an01447b

Hongyan Bi, Mingyue Yu, Yunxing Li, Yuean Chu

{"title":"Direct analysis and identification of intestinal microflora of shrimp for their geographical traceability via mass spectrometry and bacterial library searching","authors":"Hongyan Bi, Mingyue Yu, Yunxing Li, Yuean Chu","doi":"10.1039/d4an01447b","DOIUrl":"https://doi.org/10.1039/d4an01447b","url":null,"abstract":"The expansion of the seafood market has led to an increased probability of food fraud. The development of rapid and reliable traceability methods for aquatic food products is of utmost importance. In this study, direct analysis and identification of the intestinal microbiota of aquatic foods were conducted. The validity of using BacteriaMS database searching for identification of bacteria was assessed and demonstrated through analyzing prepared bacterial mixtures. We focused on shrimp as a model for aquatic food products, and utilized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze the intestinal microflora of Chinese shrimp (Fenneropenaeus chinensis) collected from three different aquaculture farms in China. It was found that the most dominant bacteria found in shrimp's intestines could serve as a basis for distinguishing shrimps' geographical origin. The most dominant bacteria in the intestines varied among shrimps from different origins, but remained identical for shrimps from the same origin. The reliability of the method in tracing the geographic origin of aquatic products was further validated by analysis of black tiger shrimp (Penaeus monodon) from different origins. The findings show that the utilization of MALDI-TOF MS for the analysis of the microbial community in the intestines of shrimp samples combined with bacterial library searching can offer a rapid, accurate, and feasible method that can be employed for determining shrimps' geographical origin. The present protocol was successfully utilized for the traceability of origins of Chinese shrimp (Fenneropenaeus chinensis) and black tiger shrimp (Penaeus monodon). It is promising to extend the present protocol to other aquatic products with regional characteristics, to help combat food fraud in the aquatic products market.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"18 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near-infrared fluorescent probes based on naphthyridine derivatives for mitochondrial nucleic acid imaging† 基于萘啶衍生物的近红外荧光探针用于线粒体核酸成像

IF 3.6 3区化学

Analyst Pub Date : 2025-01-20 DOI: 10.1039/D4AN01450B

Huan Ma, Wen-Pei Ni, Qi Lin, Ru Sun and Jian-Feng Ge

引用次数: 0

Molecularly imprinted electrochemical sensor to sensitively detect tetramethylpyrazine in Baijiu 分子印迹电化学传感器对白酒中四甲基吡嗪的灵敏检测

IF 4.2 3区化学

Analyst Pub Date : 2025-01-20 DOI: 10.1039/d4an01559b

Yating Rui, Jianfeng Wu, Qunyong Tang, Juan Pu, Wangpeng Wang, Shou-Nian Ding

{"title":"Molecularly imprinted electrochemical sensor to sensitively detect tetramethylpyrazine in Baijiu","authors":"Yating Rui, Jianfeng Wu, Qunyong Tang, Juan Pu, Wangpeng Wang, Shou-Nian Ding","doi":"10.1039/d4an01559b","DOIUrl":"https://doi.org/10.1039/d4an01559b","url":null,"abstract":"Tetramethylpyrazine (TMP), a compound known for its natural health benefits, has garnered significant attention. However, current detection methods for TMP are overly expensive and cost-timing. In this study, we developed functional materials with TMP molecular recognition properties using molecularly imprinted technology. TMP does not produce electrochemical signals in the detection potential range, hexacyanoferrate was selected as a redox probe, combined with the highly conductive polymer PEDOT:PSS to enhance electrode conductivity. When coupled with the TMP specific functional materials prepared through molecular imprinting, an electrochemical sensor specifically recognizing TMP was successfully developed, and this was confirmed through characterization techniques such as ultraviolet spectroscopy and scanning electron microscopy. Additionally, optimized the crucial experimental parameters for improved performance. Under optimal conditions, the use of differential pulse voltammetry (DPV) to measure the peak currents of hexacyanoferrate showed a linear relationship with TMP concentrations from 0.50 × 10−6 to 5.00 × 10−3 M, achieving a detection limit of 2.1 × 10−7 M. This method proved effective for quantifying TMP in Baijiu samples, demonstrating good precision with relative standard deviations (RSD) ranging from 2.71% to 3.28% and recovery percentages between 95.77% and 101.88%. These results indicate the potential of the molecularly imprinted polymer (MIP) sensor for accurately measuring TMP in actual samples.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"31 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142990432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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