Amplified colorimetric detection of chloramphenicol based on gold nanoclusters and aptamer recognition-induced three-way junction

IF 3.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst Pub Date : 2025-09-24 DOI:10.1039/d5an00957j

Mei Li, Tao Du, Qingyou Cai

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Abstract

Herein, the new colorimetric aptasensor was prepared for the ultrasensitive detection of chloramphenicol (CAP) based on gold nanoclusters (AuNCs) and aptamer recognition-induced three-way junction (TWJ). In the presence of CAP, numerous TWJ structures were formed accompanied by the dissociation of trigger T. Then, lots of hairpins were dissociated from the AuNCs surface and the peroxidase-like activity of AuNCs was restored; therefore, the increased concentration of oxidized 3,3′,5,5′-tetramethylbenzidine (oxTMB) generated a strong absorbance signal. The CAP concentration was proportional to the increased oxTMB level. Thereby, the new label-free colorimetric method was proposed to quantitatively detect CAP. At optimum conditions, the proposed method exhibited two linear dynamic ranges: from 0.16 pM to 16 pM and from 0.8 nM to 0.8 µM. with a limit of detection (LOD) of 0.05 pM. Selectivity tests against other antibiotics demonstrated that the aptasensor showed an exceptional capability to specifically detect CAP. Furthermore, the effectiveness of this sensor was validated by performing recovery tests in lake water and spiked milk samples, producing recoveries of 94.38 % - 105.38 %.

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基于金纳米团簇和适体识别诱导的三路结的氯霉素放大比色检测

本文基于金纳米簇（AuNCs）和适体识别诱导的三向结（TWJ），制备了用于氯霉素（CAP）超灵敏检测的比色适体传感器。在CAP存在下，随着触发t的解离，形成了大量的TWJ结构，大量发夹从aunc表面解离，恢复了aunc的过氧化物酶样活性；因此，氧化3,3 ',5,5 ' -四甲基联苯胺（oxTMB）浓度的增加产生了强的吸光度信号。CAP浓度与oxTMB的升高成正比。因此，提出了一种新的无标记比色法定量检测CAP。在最佳条件下，该方法具有两个线性动态范围：0.16 pM至16 pM和0.8 nM至0.8µM。检测限（LOD）为0.05 pM。对其他抗生素的选择性测试表明，该传感器具有出色的特异性检测CAP的能力。此外，通过在湖水和加标牛奶样品中进行回收率测试，验证了该传感器的有效性，回收率为94.38% ~ 105.38%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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