IF 4.2 3区化学

Analyst Pub Date : 2026-11-05 DOI: 10.1039/d6an00818f

Mohammed Adnan Abdulsalam Abdulsalam,Halil Duyar,Ferda Hacıvelioğlu,‪Burak Derkuş‬,Jonathan L. Sessler,Ergin Yalcin,Mehmet Gokhan Caglayan

{"title":"Coumarin-Quinolinium Based Fluorescent Probe for Free Thiol Detection and Cancer Cell Imaging","authors":"Mohammed Adnan Abdulsalam Abdulsalam,Halil Duyar,Ferda Hacıvelioğlu,‪Burak Derkuş‬,Jonathan L. Sessler,Ergin Yalcin,Mehmet Gokhan Caglayan","doi":"10.1039/d6an00818f","DOIUrl":"https://doi.org/10.1039/d6an00818f","url":null,"abstract":"A coumarin-quinolinium hybrid fluorescent probe was designed and synthesized through a three-step sequence. The probe exhibited moderate emission at 497 nm when excited at 407 nm and demonstrated an increase in fluorescence intensity upon reaction with free thiols in aqueous media (e.g., 5% DMSO in pH7 Britton-Robinson buffer, v/v). Additionally, its effective cell-staining capability facilitated successful application in cell imaging. The probe was found to display enhanced fluorescence in cancerous lung cells (A549) and colon cells (HT-29) relative to healthy lung cells (BEAS-2B) and colon cells (FHC). The probe thus permitted discrimination of cancer cells from healthy cells, a finding ascribed primarily to interaction and reaction with glutathione. The analytical performance of the probe was further studied, and it was found to exhibit a linear response to cysteine and glutathione over a wide concentration range. Accuracy, precision, and sustained performance were evidenced by recovery rates close to 100% and low relative standard deviation (RSD) values under conditions of repeated use. The present probe also permitted detection of the presence and absence of free thiols in two representative proteins, human serum albumin and alpha-synuclein, respectively, where it was found to provide confirmatory support for their proposed tertiary structures.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"13 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149360036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fully synthetic electrochemical sensing platform integrating bacteria-imprinted recognition with a covalent organic framework-based nanoprobe for serotype-selective detection of Escherichia coli O157:H7. 基于细菌印迹识别和共价有机框架的纳米探针的电化学传感平台，用于大肠杆菌O157:H7的血清型选择性检测。

IF 3.6 3区化学

Analyst Pub Date : 2026-11-04 DOI: 10.1039/d6an01187j

Shuang Sun, Xinying Jiang, Fei Wang, Miaozi Yu, Chunping Jia, Andrew M Hemmings, Xiaojun Bian, Jianlong Zhao

{"title":"A fully synthetic electrochemical sensing platform integrating bacteria-imprinted recognition with a covalent organic framework-based nanoprobe for serotype-selective detection of Escherichia coli O157:H7.","authors":"Shuang Sun, Xinying Jiang, Fei Wang, Miaozi Yu, Chunping Jia, Andrew M Hemmings, Xiaojun Bian, Jianlong Zhao","doi":"10.1039/d6an01187j","DOIUrl":"https://doi.org/10.1039/d6an01187j","url":null,"abstract":"Selective detection of foodborne pathogens at the serotype level remains challenging because closely related strains often share similar physicochemical features. Herein, we report a fully synthetic sandwich-type electrochemical sensor for Escherichia coli O157:H7 detection. The sensor integrates a bacteria-imprinted polymer (BIP) capture interface with a multifunctional COF-Au-MB-MPBA nanoprobe, in which a covalent organic framework (COF) is functionalized with Au nanoparticles, methylene blue (MB), and 4-mercaptophenylboronic acid (4-MPBA). The BIP layer enables primary recognition through morphological and surface-chemical complementarity, while 4-MPBA on the nanoprobe provides secondary affinity recognition of bacterial surface glycans via boronate-diol interactions. The COF-Au scaffold accommodates affinity and signaling components within a single nanoprobe, enabling direct electrochemical signal readout. With a total assay time of approximately 1.5 h, the sensor achieves a detection limit of 3 CFU mL-1 over a linear range of 101-105 CFU mL-1. Notably, it can distinguish E. coli O157:H7 from E. coli O6 even when the latter is present at a 100-fold higher concentration, demonstrating serotype-level selectivity. The assay also enables detection down to 10 CFU mL-1 in milk, apple juice, and chicken breast samples with only simple sample pretreatment. This fully synthetic sensing strategy provides a promising approach for the rapid and cost-effective detection of foodborne pathogens.","PeriodicalId":63,"journal":{"name":"Analyst","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying sequencing artifacts in low-diversity, short-insert aptamer libraries on the Illumina MiSeq platform 在Illumina MiSeq平台上鉴定低多样性、短插入的适体文库中的测序产物

IF 4.2 3区化学

Analyst Pub Date : 2026-11-03 DOI: 10.1039/d6an01029f

Mary Catherine Adams,Naima Djeddar,Anton Bryksin,Valeria Tohver Milam

{"title":"Identifying sequencing artifacts in low-diversity, short-insert aptamer libraries on the Illumina MiSeq platform","authors":"Mary Catherine Adams,Naima Djeddar,Anton Bryksin,Valeria Tohver Milam","doi":"10.1039/d6an01029f","DOIUrl":"https://doi.org/10.1039/d6an01029f","url":null,"abstract":"Next-generation sequencing (NGS) of DNA aptamer screening libraries on the popular Illumina MiSeq platform revealed unexpected artifacts affecting data quality and sequencing efficiency. These 80-base long library sequences formed adapter-ligated side products, complicating size-based purification for separating the desired adapter ligation product from the undesired side product. Among these side products, low-input samples exhibited disproportionately high adapter trimer formation (40–50% vs. ∼1% in other samples) which resulted in overestimation of sample concentration and, in turn, decreased sequencing yield. Sequencing runs displayed highly variable pass rates (6–93%), likely due to short sequences outcompeting longer ones and assignment errors of neighboring clusters with identical early-cycle bases. Unexpected duplicates in template samples suggest that adapter-ligated daughter strands and optical misidentifications contribute to artificial duplication. These duplication artifacts are of particular importance in amplicon sequencing for which copy-number analysis is crucial. These findings highlight the need for refined ligation conditions, improved purification strategies, and enhanced bioinformatic filtering to ensure sequencing accuracy in aptamer screening library workflows on the Illumina platform.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"18 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149305083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence Sensor Based on N-Doped Carbon Dots@Molecularly Imprinted Polymers for Detection of Ethyl Carbamate 基于n掺杂碳Dots@Molecularly印迹聚合物荧光传感器检测氨基甲酸乙酯

IF 4.2 3区化学

Analyst Pub Date : 2026-11-03 DOI: 10.1039/d6an01188h

Huixin Yu,Xin Yang,Xiaoxia Li,Pan Zhen,Ying Han,Xin Zhang,Maosen Yuan

{"title":"Fluorescence Sensor Based on N-Doped Carbon Dots@Molecularly Imprinted Polymers for Detection of Ethyl Carbamate","authors":"Huixin Yu,Xin Yang,Xiaoxia Li,Pan Zhen,Ying Han,Xin Zhang,Maosen Yuan","doi":"10.1039/d6an01188h","DOIUrl":"https://doi.org/10.1039/d6an01188h","url":null,"abstract":"Ethyl carbamate (EC) is a class 2A carcinogen widely generated during the fermentation of alcoholic beverages, condiments and other foods, which poses severe threats to human health. Traditional detection methods for EC suffer from complicated operation, high cost and tedious sample pretreatment. Herein, a fluorescence sensor based on nitrogen-doped carbon dots encapsulated within molecularly imprinted polymers (N-CDs@MIPs) was fabricated for the specific detection of EC. The N-CDs with excellent fluorescence performance were synthesized via a facile hydrothermal method and served as fluorescent signal probes. Molecularly imprinted polymers were prepared by a sol-gel strategy, using 3-aminopropyltriethoxysilane (APTES) as functional monomer and tetraethyl orthosilicate (TEOS) as cross-linker. The fluorescence of N-CDs@MIPs was quenched after specifically binding with EC via photoinduced electron transfer (PET) effect. Benefiting from the specific recognition of MIPs and outstanding optical characteristics of N-CDs, the proposed sensor possessed high sensitivity, excellent selectivity and strong anti-interference ability. The linear range of detecting for EC was 1-100 μg/L, and the LOD was calculated to be 0.9412 μg/L. The sensor was successfully applied to detect EC in actual wine samples, with recoveries ranging from 100.70% to 104.12% and relative standard deviations (RSD%) below 5%. This work provides a low-cost, convenient and rapid detection strategy for EC, and shows great application potential in food safety monitoring.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"34 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149305081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a dual-readout growth-based viability assay with optical density and fluorescence for assessment of nanomaterial toxicity 开发一种基于光密度和荧光的双读数生长活力测定法，用于评估纳米材料的毒性

IF 4.2 3区化学

Analyst Pub Date : 2026-11-03 DOI: 10.1039/d6an01004k

Stephanie L. Mitchell,Natalie V. Hudson-Smith,Beza Tuga,Timmy B. Nguyen,Radwa Abdelaziz,Erin E. Carlson,Christy L. Haynes

{"title":"Development of a dual-readout growth-based viability assay with optical density and fluorescence for assessment of nanomaterial toxicity","authors":"Stephanie L. Mitchell,Natalie V. Hudson-Smith,Beza Tuga,Timmy B. Nguyen,Radwa Abdelaziz,Erin E. Carlson,Christy L. Haynes","doi":"10.1039/d6an01004k","DOIUrl":"https://doi.org/10.1039/d6an01004k","url":null,"abstract":"Engineered nanomaterials can disrupt microbial communities through dysbiosis, with potentially harmful consequences. However, existing methods for assessing nanomaterial toxicity on bacterial viability are largely restricted to single-readout approaches based on bacterial density, limiting their throughput, sensitivity, and analytical scope. Herein, we present the method optimization for converting a growth-based viability assay to a dual-readout functionality that combines optical density and fluorescence measurements to enable more comprehensive and high-throughput bacterial toxicity screening. Method optimization addressed fluorescence signal acquisition, effects of selection antibiotics, and automated R-code data analysis. As a proof of concept, we evaluated the toxicity of positively-charged nanodiamonds against a green fluorescent protein-expressing Shewanella oneidensis. This dual-readout platform expands the utility of growth-based viability assays by enabling simultaneous assessment of bacterial viability alongside genetic events (e.g., horizontal gene transfer, gene expression, gene copy number), and facilitates future polymicrobial experimental designs, offering a versatile tool for nanomaterial toxicity research.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"6 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149305082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Cloud Point Extraction Methods with Two Independent Approaches for Selective Preconcentration and Determination of Pb(II) or Cu(II) in Water and Fruit Samples 水和水果样品中Pb（II）或Cu（II）的两种独立云点提取方法

IF 4.2 3区化学

Analyst Pub Date : 2026-10-30 DOI: 10.1039/d6an01042c

Olcay Ozdemir,Celal Duran,Hakan Bektas,Duygu Ozdes

{"title":"Novel Cloud Point Extraction Methods with Two Independent Approaches for Selective Preconcentration and Determination of Pb(II) or Cu(II) in Water and Fruit Samples","authors":"Olcay Ozdemir,Celal Duran,Hakan Bektas,Duygu Ozdes","doi":"10.1039/d6an01042c","DOIUrl":"https://doi.org/10.1039/d6an01042c","url":null,"abstract":"In this study, two independent cloud point extraction (CPE) methods were established for the selective separation and preconcentration of Pb(II) or Cu(II) ions using benzimidazole derivative ligands, which are employed for the first time for analytical purposes. The 2-cyclopropyl-1H-benzo[d]imidazole (CPBI) ligand was adopted for the selective extraction of Pb(II) ions, while the 2-methyl-1H-benzo[d]imidazole (MEBI) ligand was utilized for Cu(II) extraction. During method development, parameters affecting extraction efficiency, such as pH, amount of chelating agent, surfactant (Triton X-114) concentration, incubation temperature and time, and centrifugation speed and time, were thoroughly examined. Under optimum conditions, quantitative recoveries were attained for Pb(II) and Cu(II) ions at pH values of 8.0 and 7.0, respectively. The limits of detection (LODs) were 0.77 µg L-1 and 0.84 µg L-1 for Pb(II) and Cu(II), respectively, with a preconcentration factor of 25 for both methods. The intraday RSD values were 1.6% and 3.9%, while the interday RSD values were 2.4% and 4.3% for Pb(II) and Cu(II), respectively. The accuracy of the procedures was evaluated via the addition/recovery experiments on real sample matrices, which demonstrated recovery values within acceptable limits. The proposed methods were successfully applied to solid samples, including pear, apricot, carrot for the determination of Pb(II) ions, and cherry, peach, and apple for Cu(II) determination, as well as to various water samples. All analyses were carried out using flame atomic absorption spectrometry (FAAS). The greenness of the proposed methods was also evaluated using the Analytical GREEnness (AGREE) metric, yielding an overall score of 0.41. In summary, this study establishes the feasibility of benzimidazole derivatives as ligands in CPE-based metal analysis and introduces a new analytical method for the sensitive determination of Pb(II) and Cu(II) in water and food samples by using economical reagents.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"76 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149303285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pesticide Detection Using Core-shell Nanoparticles Coated Optical Fiber Sensor in Environmental Sample 核壳纳米粒子包覆光纤传感器在环境样品中的农药检测

IF 4.2 3区化学

Analyst Pub Date : 2026-10-27 DOI: 10.1039/d6an00655h

Akanksha Mishra,Rajesh K. Gangwar,Roli Verma

{"title":"Pesticide Detection Using Core-shell Nanoparticles Coated Optical Fiber Sensor in Environmental Sample","authors":"Akanksha Mishra,Rajesh K. Gangwar,Roli Verma","doi":"10.1039/d6an00655h","DOIUrl":"https://doi.org/10.1039/d6an00655h","url":null,"abstract":"Nowadays, the use of pesticides is widespread to increase crop yield and quality. However, it contaminates water, food, and soil and threatens humans and ecosystems due to its bioaccumulation and persistence. The food we eat is grown using pesticides, harming human health. Therefore, developing a cost-effective, rapid and sensitive monitoring protocol for detecting pesticides in water and soil is highly desirable, especially since rice is widely consumed and produced in India. In this study, core-shell structured copper – silver nanoparticles (Cu-Ag CSNPs) were chemically synthesized with a two-step process. The CSNPs were then thoroughly characterized using XRD, SEM, EDS, TEM, XPS, FTIR, and UV-Vis. spectroscopy. These CSNPs were then exploited as a plasmonic sensing unit to design a fiber sensor for detecting imidacloprid (IMD) pesticides. Due to strong chemical interaction between CSNPs and IMD, distinct spectral shifts were observed over a vast range of concentrations (1200µM-0.001µM) due to the LSPR effect. The developed sensor has been employed for both laboratory and real sample analyses. Also demonstrating successful selectivity in real environmental matrices like river water, rice, and agricultural soil, and achieved a high recovery percentage. The novelty of this work lies in the first integration of cu-Ag CSNPs into an LSPR-based optical fiber platform for ultra-trace IMD detection, achieving a detection limit of picomolar level.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"79 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149250980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Gold Immunochromatographic Strip with Broad-Specificity Monoclonal Antibodies for Rapid Detection of Matrine-Type Alkaloids in Honey 快速检测蜂蜜中苦参型生物碱的单克隆抗体金免疫层析条带的建立

IF 4.2 3区化学

Analyst Pub Date : 2026-10-23 DOI: 10.1039/d6an01015f

Shufen Si,Xueqing Wang,Liqiang Liu,aihong wu,Chuanlai Xu

{"title":"Development of a Gold Immunochromatographic Strip with Broad-Specificity Monoclonal Antibodies for Rapid Detection of Matrine-Type Alkaloids in Honey","authors":"Shufen Si,Xueqing Wang,Liqiang Liu,aihong wu,Chuanlai Xu","doi":"10.1039/d6an01015f","DOIUrl":"https://doi.org/10.1039/d6an01015f","url":null,"abstract":"Matrine-type alkaloids (MTAs) occur naturally in Sophora plants and frequently contaminate honey, creating regulatory friction between China and the EU owing to their toxicity and divergent maximum residue limits. Existing analytical tools either rely on laboratory-based instrumentation or target single analytes. We developed a colloidal gold immunochromatographic strip (GICA) that simultaneously detects four MTAs-oxymatrine, matrine, sophocarpine and sophoridine-in honey. A broad-specificity monoclonal antibody (mAb 1C1) was raised via computationally guided hapten design, integrating conformational overlay, Mulliken charge and electrostatic potential mapping. The antibody showed high affinity (Kₐ = 3.85 × 10¹⁰ L/mol) and half-maximal inhibitory concentrations of 1. 73, 3.84, 3.77 and 22.43 ng/mL for the four alkaloids, respectively. Under optimised conditions, the strip achieved visual cut-offs of 2-50 mg/kg and calculated LODs of 0.03-0.42 mg/kg, with spike recoveries of 87.2-107.0% in honey. Results agreed well with LC-MS/MS and the total assay time was 15 min. This work provides a simple, low-cost, field-ready platform for multi-alkaloid screening, directly addressing the compliance needs of honey export monitoring.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"4 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149241312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laser-induced breakdown spectroscopy for food safety analysis: sample presentation, data interpretation, and application readiness 用于食品安全分析的激光诱导击穿光谱：样品呈现、数据解释和应用准备

IF 4.2 3区化学

Analyst Pub Date : 2026-10-22 DOI: 10.1039/d6an00368k

Yikang Hou,Zhuoxi Li,Jie Lian

{"title":"Laser-induced breakdown spectroscopy for food safety analysis: sample presentation, data interpretation, and application readiness","authors":"Yikang Hou,Zhuoxi Li,Jie Lian","doi":"10.1039/d6an00368k","DOIUrl":"https://doi.org/10.1039/d6an00368k","url":null,"abstract":"Food-safety decisions require rapid screening of adulteration, origin fraud, elemental contamination and freshness loss. Laser-induced breakdown spectroscopy (LIBS) provides direct multi-element fingerprints with short acquisition times and little reagent consumption. Its performance in foods, however, depends on a coupled analytical chain: sample presentation controls laser coupling and mass removal; plasma evolution and acquisition govern emission; calibration and modelling determine interpretation; and validation establishes reliability beyond the development dataset. This critical review uses that chain to assess food-related LIBS workflows. It examines powders, moist tissues, fatty materials, liquids and spatially heterogeneous samples, then considers plasma-aware acquisition, preprocessing, chemometrics, deep learning and multimodal fusion. Task-specific comparisons with ICP-based methods, near-infrared spectroscopy, Raman spectroscopy and hyperspectral imaging clarify where LIBS adds value. Pelletization provides a reproducible baseline for many powders, whereas moist, fatty and liquid matrices often require flattening, phase control, immobilization or enrichment. Readiness is strongest for selected mineral-related authenticity screens and elemental warning at concentrations clearly above a decision threshold, provided that independent validation and quality control are available. Closed-set provenance and portable sorting are generally less mature, while regulatory trace confirmation, cross-instrument transfer and realistic microbial analysis require stronger evidence. Progress depends on end-to-end timing, threshold-focused validation, interpretable models, drift monitoring and selective confirmation with complementary techniques.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"9 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149230136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of drug concentrations in individual living cancer cells using Raman spectroscopy and machine learning 利用拉曼光谱和机器学习对单个活癌细胞中的药物浓度进行定量

IF 4.2 3区化学

Analyst Pub Date : 2026-10-22 DOI: 10.1039/d6an00870d

Jiaming Li,Jan Cerski,Andrew Downes

{"title":"Quantification of drug concentrations in individual living cancer cells using Raman spectroscopy and machine learning","authors":"Jiaming Li,Jan Cerski,Andrew Downes","doi":"10.1039/d6an00870d","DOIUrl":"https://doi.org/10.1039/d6an00870d","url":null,"abstract":"In anticancer drug discovery, there is a clear need to determine how much of a drug enters the cell and its concentration in different cellular compartments (e.g., the nucleus or cytoplasm). This information helps assess therapeutic efficacy and optimise drug design and in vivo dosage. Compared with other analytical techniques, Raman spectroscopy provides many advantages that make it well suited to this application. It is non-destructive, preserving the structural and chemical integrity of the sample. It also eliminates the need for complex sample preparation and is suitable for live-cell experiments. Moreover, Raman spectra are largely unaffected by water interference, making the technique ideal for analysing living cells. Label-free detection is one of its most distinctive advantages. This study requires quantitative analysis without any fluorescent labels or other biological markers, while Raman measurements can rely solely on the inherent Raman properties of the anticancer drug itself, eliminating potential interference from labels on biological activity. Overall, this paper provides a comprehensive investigation into the application of Raman spectroscopy for quantifying anticancer drug concentrations in living cells, combined with machine learning-based analysis. It advances beyond imaging analysis of drug distribution by determining drug concentrations and membrane permeability coefficients using Raman signals. For drugs administered at a concentration of 0.3%, accuracies of 0.01-0.1% were determined for a variety of anticancer drugs in different cell lines within a measurement window of 5 minutes.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"1 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2026-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149230137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyst最新文献