IF 4.2 3区化学

Analyst Pub Date : 2025-07-23 DOI: 10.1039/d5an00487j

Harshitha Govindaraju, Muhammad Nabeel Tahir, Umer Hassan

{"title":"HIST-DIP: Histogram Thresholding and Deep Image Priors assisted Smartphone-Based Fluorescence Microscopy Imaging","authors":"Harshitha Govindaraju, Muhammad Nabeel Tahir, Umer Hassan","doi":"10.1039/d5an00487j","DOIUrl":"https://doi.org/10.1039/d5an00487j","url":null,"abstract":"Portable fluorescence microscopes coupled with smartphones offer accessible and cost-effective point- of-care diagnostic solutions, but often produce noisy and blurry images with poor contrast. Here, we introduce HIST-DIP (HIStogram Thresholding and Deep Image Prior), an unsupervised framework for fluorescence microscopy image restoration. Histogram thresholding isolates fluorescence signals by removing background noise, while DIP refines structural details and enhances resolution without large labeled datasets. Validation results show substantial quality gains including the average Peak Signal-to-Noise Ratio (PSNR) improved from 15.59dB to 27.10dB, and the Structural Similarity Index Measure (SSIM) rose from 0.035 to 0.82. Contrast-to-noise ratio (CNR) and signal difference- to-noise ratio (SDNR) also increased significantly, indicating sharper bead outlines and reduced background interference. Unlike conventional deep learning methods, HIST-DIP needs no external training data, making it well-suited for real-time, low-cost, and point-of-care diagnostic imaging. These findings highlight the potential of HIST-DIP in enhancing the quality of smartphone-based microscopy images, while also motivating future research towards optimizing the methods for real- time on-device computations.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"108 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A holistic approach to understanding biochemical degradation of human tissues using high resolution MALDI MS 使用高分辨率MALDI质谱分析理解人体组织生化降解的整体方法

IF 4.2 3区化学

Analyst Pub Date : 2025-07-18 DOI: 10.1039/d5an00588d

Jerika Ho, Naomi Stock, Vaughn Mangal, Shari Forbes, Theresa Stotesbury

{"title":"A holistic approach to understanding biochemical degradation of human tissues using high resolution MALDI MS","authors":"Jerika Ho, Naomi Stock, Vaughn Mangal, Shari Forbes, Theresa Stotesbury","doi":"10.1039/d5an00588d","DOIUrl":"https://doi.org/10.1039/d5an00588d","url":null,"abstract":"Estimating the postmortem interval (PMI) is crucial in medico-legal death investigations, but existing methods are highly influenced by environmental and physiological factors. This study proposes a novel biochemical strategy for PMI estimation using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI MS) to detect molecular changes in decomposing human soft tissues. Samples from the quadriceps and shoulder were collected from 13 human donors at the Research in Experimental and Social Thanatology (REST) facility in Quebec, Canada, spanning accumulated degree days (ADD) ranging from 9.75 to 11,455.86. Tissues (14 µm) were cryosectioned, mounted on carbon tape, dried under nitrogen gas, and coated with a 2,5-dihydroxybenzoic acid matrix solution. Hematoxylin and eosin staining provided histological context for the sampled tissues. MALDI MS data were acquired using a Bruker SolariX XR Fourier-transform Ion Cyclotron Resonance MS. Van Krevelen diagrams and principal component analysis revealed decomposition-linked trends, with shifts in biochemical profiles over time. N/C and N/O ratios increased, reflecting protein and peptide degradation and microbial activity, while O/C ratios declined, likely due to the loss of oxygen-rich compounds. Mixed-effects models showed moderate associations (R² marginal = 0.06–0.49) between elemental ratios and ADD, with conditional R² values up to 0.55. Spearman’s rank correlation identified compounds significantly associated with ADD. Six candidate biomarkers were identified, with variable model fit (R² marginal = 0.003–0.80) and unresolved donor-level effects. This study demonstrates the novel application of MALDI MS for PMI biomarker discovery and contributes one of the largest datasets in forensic decomposition chemistry research.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"7 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a light-initiated chemiluminescence system for the detection of human plasma p-tau181 人血浆p-tau181光激发化学发光检测系统的研制

IF 4.2 3区化学

Analyst Pub Date : 2025-07-18 DOI: 10.1039/d5an00590f

yang yang, Xu Wang

{"title":"Development of a light-initiated chemiluminescence system for the detection of human plasma p-tau181","authors":"yang yang, Xu Wang","doi":"10.1039/d5an00590f","DOIUrl":"https://doi.org/10.1039/d5an00590f","url":null,"abstract":"Phosphorylated tau 181 (p-tau181) is a promising biomarker for predicting and monitoring Alzheimer’s disease (AD) neuropathology. However, existing commercial assays suffer from disadvantages such as high cost and insufficient detection sensitivity, limiting their clinical applications and suitability for routine laboratory use. In this study, we developed a novel light-initiated chemiluminescence assay (LiCA®) and evaluated its performance for quantifying plasma p-tau181 levels. A compact, fully automated LiCA® analyzer was developed for decentralized and point-of-care testing environments. Analytical performance of the p-tau181 assay on the analyzer was validated according to Clinical and Laboratory Standards Institute (CLSI) guidelines, with evaluation of precision, accuracy, linearity, detection capability, and interference. Reference intervals were established per EP28-A3c. Method comparison was conducted using Spearman’s correlation with the Simoa® pTau-181 Advantage V2.1 kit. The assay demonstrated excellent precision (coefficient of variation: 2.72–5.11%), high recovery accuracy (93.29–107.56%), and strong linearity (R = 0.9986) across a range of 0–200 pg/mL. Sensitivity parameters included limit of blank (LoB)=0.10 pg/mL, limit of detection (LoD)=0.19 pg/mL, limit of quantitation (LoQ)=0.30 pg/mL (20% CV) and LoQ=0.57 pg/mL (10% CV). Plasma p-tau181 was detectable in 100% of healthy individuals (n = 400), confirming the assay’s capability for baseline differentiation. Strong correlation with the Simoa® platform (ρ = 0.868) further validated clinical concordance. LiCA® offers a fully automated, random-access platform for highly sensitive and accurate measurement of p-tau181. It could be a viable tool for early screening and differential diagnosis of neurodegenerative diseases in both clinical trial settings and routine clinical practice.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"677 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PAM-Free Activation of CRISPR/Cas12a via Semi-Nested Asymmetric RPA: High Specific Detection of HPV16 dsDNA 通过半嵌套不对称RPA无pam激活CRISPR/Cas12a：高特异性检测HPV16 dsDNA

IF 4.2 3区化学

Analyst Pub Date : 2025-07-16 DOI: 10.1039/d5an00491h

Xiaozhi Zou, Tao Gu, Xuheng Li, Liyuan Deng, Shuyu Zhu, Jiangbo Dong, Fei Deng, Danqun Huo, Changjun Hou

{"title":"PAM-Free Activation of CRISPR/Cas12a via Semi-Nested Asymmetric RPA: High Specific Detection of HPV16 dsDNA","authors":"Xiaozhi Zou, Tao Gu, Xuheng Li, Liyuan Deng, Shuyu Zhu, Jiangbo Dong, Fei Deng, Danqun Huo, Changjun Hou","doi":"10.1039/d5an00491h","DOIUrl":"https://doi.org/10.1039/d5an00491h","url":null,"abstract":"Early and accurate detection of HPV16 nucleic acids is therefore critical for the effective screening, diagnosis, and prevention of cervical cancer. Although CRISPR/Cas12a-based molecular diagnostics offer a rapid and sensitive approach for HPV16 detection, their application to double-stranded DNA (dsDNA) targets remain constrained by two major limitations: the strict requirement for a protospacer adjacent motif (PAM) site, and the insufficient specificity of current amplification strategies, which can lead to off-target amplification and false-positive results. To address these challenges, we developed a semi-nested asymmetric recombinase polymerase amplification (SNA-RPA) method combined with CRISPR/Cas12a for the detection of HPV16 dsDNA. This strategy employs a semi-nested primer design to significantly enhance target sequence specificity during amplification, while asymmetric primer ratios promote the efficient generation of single-stranded DNA (ssDNA) that directly activates Cas12a without the need for a PAM site. Using this approach, we achieved rapid, highly specific, and detection of HPV16 dsDNA, with a limit of detection as low as 18 aM. Beyond achieving PAM-free detection, our method also substantially improves amplification fidelity, offering a promising solution for precise and reliable HPV diagnostics and cervical cancer screening","PeriodicalId":63,"journal":{"name":"Analyst","volume":"16 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144640380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

X-ray Induced Modifications in U87 Glioma Cells Probed by Raman- and Infrared-Based Spectromicroscopy 用拉曼和红外光谱显微镜观察x射线诱导的U87胶质瘤细胞的修饰

IF 4.2 3区化学

Analyst Pub Date : 2025-07-16 DOI: 10.1039/d5an00399g

Tamasri Senapati, Marius R. Bittermann, Robin Abraham Nadar, Astrid van de Meer, Bernd Kästner, Antonia Georgieva Denkova, E. Ruhl

{"title":"X-ray Induced Modifications in U87 Glioma Cells Probed by Raman- and Infrared-Based Spectromicroscopy","authors":"Tamasri Senapati, Marius R. Bittermann, Robin Abraham Nadar, Astrid van de Meer, Bernd Kästner, Antonia Georgieva Denkova, E. Ruhl","doi":"10.1039/d5an00399g","DOIUrl":"https://doi.org/10.1039/d5an00399g","url":null,"abstract":"A combination of spontaneous Raman, stimulated Raman, and photothermal expansion (AFM-IR) spectromicroscopy is reported for probing the impact of different radiation doses (2-10 Gy) on U87 glioma cells ex vivo. Most significant are alterations in spectral profiles caused by radiation-induced changes, while keeping the cell fixation delay constant at 24 h. The changes in delay of the fixation ranging up to 5 d at a dose of 2 Gy were also investigated for probing cellular recovery processes of exposed cells. Both, the Raman-based and AFM-IR hyperspectral analyses identified statistically significant spectral changes and radiation-induced alterations in cellular proteins, nucleic acids, and lipids. Specifically, these label-free approaches revealed a 3 fold and 2-fold decrease in nucleic acid and lipid content,-respectively, for cells treated with 10 Gy compared to untreated control samples. This study unravels the potential of a combination of Raman-based approaches and AFM-IR that is of use for therapeutics and offers a Label-free mapping of cetuximab in multi-layered tumor oral mucosa models by atomic force-microscopy-based infrared spectroscopy.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"16 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144640412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel cataluminescence sensor for rapid detection of methanol at low working temperature based on Ni/CeO2 catalyst 基于Ni/CeO2催化剂的低温快速检测甲醇的新型催化发光传感器

IF 4.2 3区化学

Analyst Pub Date : 2025-07-15 DOI: 10.1039/d5an00626k

Shufang Luo, Fangyuan Yuan, Zhenhua Qin, Yuxiu Yang, Xu Teng, Chao Lu

{"title":"A novel cataluminescence sensor for rapid detection of methanol at low working temperature based on Ni/CeO2 catalyst","authors":"Shufang Luo, Fangyuan Yuan, Zhenhua Qin, Yuxiu Yang, Xu Teng, Chao Lu","doi":"10.1039/d5an00626k","DOIUrl":"https://doi.org/10.1039/d5an00626k","url":null,"abstract":"Cataluminescence (CTL) is an attractive chemiluminescence phenomenon that occurs at the gas-solid catalytic interface, and there is a strong anticipation for highly efficient CTL. Herein, Ni/CeO2 catalyst was successfully developed by using the in-situ doping method. In comparison with the CeO2 nanoparticles and NiO nanoparticles, Ni/CeO2 catalyst exhibited superior CTL performance toward methanol. Based on the outstanding catalytic performance of Ni/CeO2, a novel CTL sensor with excellent sensitivity, good selectivity, and satisfactory stability was fabricated for the detection of methanol at low working temperature. Notably, the reaction time and recovery time of the proposed CTL sensor for methanol detection were as short as 7 s and 6 s, respectively. The proposed CTL sensor was successfully applied to detect methanol in commercial white wine samples with recoveries of 107.86-113.09%. The relative standard deviation for the detection of methanol in commercial white wine samples was less than 7%. Accordingly, the proposed CTL sensor with the advantage of fast response and reliable characteristics provided a promising choice for rapid detection of methanol. This work provided a new direction for the development of CTL sensors with high performance and low working temperature.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"51 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144630126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A minimal sampling, in-line spectroscopic calibration method for unstable components during ammoniation of fatty acids 脂肪酸氨化过程中不稳定组分的最小采样，在线光谱校准方法

IF 4.2 3区化学

Analyst Pub Date : 2025-07-15 DOI: 10.1039/d4an01051e

C. M. Raffel, J. Meekes, H.-J. van Manen, A. J. B. ten Kate, A. Chaudhuri, J. van der Schaaf

{"title":"A minimal sampling, in-line spectroscopic calibration method for unstable components during ammoniation of fatty acids","authors":"C. M. Raffel, J. Meekes, H.-J. van Manen, A. J. B. ten Kate, A. Chaudhuri, J. van der Schaaf","doi":"10.1039/d4an01051e","DOIUrl":"https://doi.org/10.1039/d4an01051e","url":null,"abstract":"The ammoniation of fatty acid produces fatty amide, as well as unstable ammonium salt, whose composition profile can vary with off-line sampling. This makes the analysis and determination of reaction kinetics challenging. In-line FT-IR spectroscopy removes the need for sampling, but requires calibration of the reacting system at reaction conditions, which is complicated by the near-instant formation of ammonium salt. In this work, we demonstrate the development of a calibration method which overcomes the complexities posed by both off-line and in-line analysis. This is achieved by taking only eight off-line samples for determination of the more stable components and formulating mass balances of the reactive system for each in-line acquired FT-IR spectrum. This allows for the determination of the rapidly fluctuating salt content. The essential assumptions underlying the mass balances and the resulting calibration are based on a qualitative examination of the reaction system through principal component analysis. Put together, this enables in-line calibration of the system at reaction conditions, achieving relative errors below 10% using partial least squares regression.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"199 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144629999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing ssRNA and dsRNA electrophoretic behavior: empirical insights with neural network-aided predictions 表征ssRNA和dsRNA电泳行为：与神经网络辅助预测的经验见解

IF 4.2 3区化学

Analyst Pub Date : 2025-07-15 DOI: 10.1039/d5an00381d

Nina Sheng Li, Adriana Coll De Peña, Matei Vaduva, Somdatta Goswami, Anubhav Tripathi

{"title":"Characterizing ssRNA and dsRNA electrophoretic behavior: empirical insights with neural network-aided predictions","authors":"Nina Sheng Li, Adriana Coll De Peña, Matei Vaduva, Somdatta Goswami, Anubhav Tripathi","doi":"10.1039/d5an00381d","DOIUrl":"https://doi.org/10.1039/d5an00381d","url":null,"abstract":"RNA-based therapeutics are currently at the forefront of the biopharmaceutical industry because of their safety, efficacy, and shortened time from disease discovery to therapy development. Microfluidic electrophoresis provides a great analytical platform to analyze nucleic acids in unprecedented detail. However, while DNA has been studied extensively within microfluidic systems, there is limited data available for RNA, particularly of chemically modified molecules, such as those used in the COVID-19 mRNA vaccines, and for long double-stranded RNA molecules, which may accompany, intentionally or as a by-product, RNA therapeutics. To this end, this study focused on the empirical microfluidic electrophoretic analysis of double- and single-stranded RNA, non-modified and pseudouridine-modified, at varying gel concentrations. It then compared the findings to the electrophoretic mobility models in the literature. This work was then complemented with data-driven and physics-informed neural networks that successfully predicted the migration time and length of different RNA molecules with an average error of 12.34% for the data-driven model and 0.77% for the physics-informed model. The low error in the physics-informed neural networks opens the doors to the electrophoretic characterization of molecules, even beyond RNA, without the need for extensive experimental data.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"15 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144630123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colourimetric Detection of Bisphenol A in Water: A Smartphone-Based Sensor Using Inverse Opal Molecularly Imprinted Photonic Crystal Hydrogel 水中双酚A的比色检测：基于智能手机的反蛋白石分子印迹光子晶体水凝胶传感器

IF 4.2 3区化学

Analyst Pub Date : 2025-07-14 DOI: 10.1039/d4an01426j

Sree Sanker SS, Subin Thomas, Dhanya P Jacob, V S Suniya, Savitha Nalini, Madhusoodanan Kottarathil Naduvil

{"title":"Colourimetric Detection of Bisphenol A in Water: A Smartphone-Based Sensor Using Inverse Opal Molecularly Imprinted Photonic Crystal Hydrogel","authors":"Sree Sanker SS, Subin Thomas, Dhanya P Jacob, V S Suniya, Savitha Nalini, Madhusoodanan Kottarathil Naduvil","doi":"10.1039/d4an01426j","DOIUrl":"https://doi.org/10.1039/d4an01426j","url":null,"abstract":"Molecularly imprinted photonic crystal hydrogel (MIPCH) serves as a highly effective platform for the sensitive and selective detection of various analyte molecules. In this study, we present a smartphone-based inverse opal MIPCH (IOMIPCH) sensor designed for the sensitive and selective detection of bisphenol A (BPA) in water samples. The sensor is prepared by photopolymerizing the hydrogel precursor solution within the voids of a polystyrene (PS) photonic crystal (PC) opal film. This is followed by the etching of BPA molecules and the removal of PS spheres forming inverse opal structure with binding sites for analyte BPA. The sensor displays a vibrant structural colour that experiences a redshift upon rebinding of the BPA molecules. The structural colour change provides a visually observable indication of the sensor response. The IOMIPCH-BPA sensor demonstrates a low limit of detection (LoD) of 0.69 fM and a rapid response time of 4 minutes with the ability to selectively detect BPA even in complex sample matrices. Additionally, it is reusable and maintains its performance for up to one month. We used the sensor response images to train a deep learning-based regression model on the smartphone, enabling quantitative predictions of BPA concentration. This integration creates an accurate, portable smart sensor platform capable of real-time BPA sensing.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"279 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144622727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation of lactose sample batches from surface impurities by OrbiSIMS OrbiSIMS对乳糖样品批次与表面杂质的鉴别

IF 4.2 3区化学

Analyst Pub Date : 2025-07-14 DOI: 10.1039/d5an00364d

Bin Yan, Junting Zhang, Eleonora Paladino, Mark Nicholas, Paulina Rakowska, Anthony W.T. Bristow, Ian Gilmore

引用次数: 0

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