IF 4.2 3区化学

Analyst Pub Date : 2025-01-28 DOI: 10.1039/d4an01467g

Fangming Zhu, Yibo Zhao, Chenji Dai, Yaoyao Xu, Yuyang Zhou

{"title":"Iridium(III) Complex Functionalized ZIF-8 as Novel POD-like Nanozyme for Visual Assay of Triazine Pesticides","authors":"Fangming Zhu, Yibo Zhao, Chenji Dai, Yaoyao Xu, Yuyang Zhou","doi":"10.1039/d4an01467g","DOIUrl":"https://doi.org/10.1039/d4an01467g","url":null,"abstract":"Due to the unique advantages of mimicking nature enzymes, nanozyme has received ever-growing interests in wide fields including analytical chemistry in the past two decades. Exploring novel kinds of nanozyme with efficient active sites is always one of the most important and hot topics in nanozyme-related research so far, especially in portable monitor . Herein, organometallic iridium(III) complex as new active site incorporated into zeolitic imidazolate framework-8 (ZIF-8) denoted as Irppy-ZIF-8 via one-pot coordination reaction between iridium solvent complex and 2-methylimidazole was reported as an efficient peroxidase (POD)-like nanozyme in this work. Importantly, due to the specific inhibition effects of triazine pesticides on the POD-like activities of this novel nanozyme, a portable acetylcholinesterase (AChE)-free colorimetric sensor via smartphone apart from UV-vis spectrometer to detect triazine pesticides in the real vegetable samples analysis was further successfully proposed in this work. It should be noted that this work could not only open up a new avenue to explore novel kinds of nanozymes from organometallic complex as active sites, but also promote the advanced progresses in the emerging applications of nanozymes in visual and portable sensors in the future.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"50 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating entropy-driven DNA circuit with tetrahedral scaffold as a generic in-situ electrochemical biosensor for amplified detection of microRNAs 将熵驱动 DNA 电路与四面体支架整合为通用原位电化学生物传感器，用于放大检测 microRNAs

IF 4.2 3区化学

Analyst Pub Date : 2025-01-28 DOI: 10.1039/d4an01528b

Xuyao Wang, Junlan Zhu, Peng Shu, Jiajing Wang, Maowen Huang, Hengchao Chen, Haifen Ma

{"title":"Integrating entropy-driven DNA circuit with tetrahedral scaffold as a generic in-situ electrochemical biosensor for amplified detection of microRNAs","authors":"Xuyao Wang, Junlan Zhu, Peng Shu, Jiajing Wang, Maowen Huang, Hengchao Chen, Haifen Ma","doi":"10.1039/d4an01528b","DOIUrl":"https://doi.org/10.1039/d4an01528b","url":null,"abstract":"Detection of carcinogenesis-related miRNAs presents significant challenges due to their low abundance and high specificity, necessitating highly sensitive and reliable analytical methods. Herein, we propose a generic in-situ electrochemical biosensor for the sensitive and effective detection of miRNAs by rationally integrating entropy-driven DNA circuit (EDC) with tetrahedral scaffold. The key advancement of this work is the implementation of tetrahedral DNA nanostructures (TDNs) as both a scaffold and substrate for the EDC directly on the electrode surface. TDNs, which are readily decorated with ordered orientation and well controlled spacing, enhance hybridization efficiency and facilitate essential structural interactions within the EDC, achieving performance comparable to that of homogeneous liquid-phase reactions. Target miRNA is identified with complementary probes, triggering a cascade of structural rearrangements that leads to the immobilization of numerous biotin-labeled signal strands on the electrode surface. This accumulation of biotinylated strands ensures that the initial interfacial hybridization event is subsequently amplified and translated into electrochemical signals via cascaded signal amplification. The resulting electrochemical signals are directly proportional to the concentration of target miRNA, offering a highly sensitive detection platform with a detection limit as low as 74 aM and a dynamic range spanning from 100 aM to 100 pM. The biosensor’s performance is validated using biological samples derived from B[a]PDE-exposed cells, where significantly elevated miR-96 levels are detected, consistent with qRT-PCR results. This demonstrates the potential of the proposed biosensor for early cancer diagnosis and monitoring of cancer-related miRNA biomarkers.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"121 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-free temperature resilient amplification assay with toehold stem-loop probe

IF 4.2 3区化学

Analyst Pub Date : 2025-01-28 DOI: 10.1039/d4an01212g

Jay Bhakti Kapadia, Jamal Daoud, Jonathan Perreault

引用次数: 0

Highly sensitive LC-MRM workflow for quantitation of efflux transporters in rat peripheral blood mononuclear cells: Leveraging ProteoExcelTP with MRM prediction capability

IF 4.2 3区化学

Analyst Pub Date : 2025-01-27 DOI: 10.1039/d4an01514b

Tarang Jadav, Niraj Rajput, Pinaki Sengupta

{"title":"Highly sensitive LC-MRM workflow for quantitation of efflux transporters in rat peripheral blood mononuclear cells: Leveraging ProteoExcelTP with MRM prediction capability","authors":"Tarang Jadav, Niraj Rajput, Pinaki Sengupta","doi":"10.1039/d4an01514b","DOIUrl":"https://doi.org/10.1039/d4an01514b","url":null,"abstract":"Though combinational antiretroviral therapy has been proven highly effective, it suffers from drug-drug interactions, drug resistance and adverse reactions upon their long-term use. Introduction of novel drugs in antiretroviral therapy proposes newer treatment options. However, drug safety and their interaction potential after long-term therapy are still unexplored. In this study, the induction potential of bictegravir on efflux transporters at protein level was assessed here by estimating the transporters by LC-MS/MS based quantitation method. Surrogate peptide approach was used for simultaneous determination of P-gp, BCRP and MRP1 transporter proteins in rat peripheral blood mononuclear cells. Previously developed excel based ProteoExcelTP tool was utilized for selection of surrogate peptides corresponding to target transporters. Moreover, ProteoExcelTP was integrated with a newer MRM prediction capability for prediction of MRM transitions of selected surrogate peptides. Surrogate peptides LLSGQALK (415.2→716.4), SSLLDVLAAR (522.8→288.1) and EDLDLVLK (472.7→685.3) were selected for P-gp, BCRP and MRP1 transporters proteins, respectively. The peptides LLSGQALK, SSLLDVLAAR and EDLDLVLK were eluted at 5.4, 7.0 and 4.1 min, respectively. The finding of the study revealed that bictegravir can significantly induce BCRP transporter after a week of its administration to Sprague Dawley rats. This finding can be utilized in future to prevent transporter mediated drug-drug interactions of bictegravir. Moreover, addition of MRM prediction feature to ProteoExcelTP has enhanced its applicability in mass spectrometry based targeted proteomics. The developed LC-MS/MS based quantitation method for determination of clinically relevant efflux transporters will be useful in investigating the induction potential of any other drug.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"13 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioanalytical method for NAD+ detection in blood plasma utilizing solution-phase Candida boidinii formate dehydrogenase and electrochemical detection

IF 4.2 3区化学

Analyst Pub Date : 2025-01-27 DOI: 10.1039/d4an01560f

Wichit Taron, Tharinda Kasemphong, Pachanuporn Sunon, Keerakit Kaewket, Nuntaporn Kamonsutthipaijit, James Robert Ketudat Cairns, Gun Bhakdisongkhram, Warut Tulalamba, Supakmongkon Sanguansuk, Vip Viprakasit, Kamonwad Ngamchuea

{"title":"Bioanalytical method for NAD+ detection in blood plasma utilizing solution-phase Candida boidinii formate dehydrogenase and electrochemical detection","authors":"Wichit Taron, Tharinda Kasemphong, Pachanuporn Sunon, Keerakit Kaewket, Nuntaporn Kamonsutthipaijit, James Robert Ketudat Cairns, Gun Bhakdisongkhram, Warut Tulalamba, Supakmongkon Sanguansuk, Vip Viprakasit, Kamonwad Ngamchuea","doi":"10.1039/d4an01560f","DOIUrl":"https://doi.org/10.1039/d4an01560f","url":null,"abstract":"Nicotinamide adenine dinucleotide is a crucial coenzyme in cellular metabolism and is implicated in various diseases. This work introduces an electrochemical bioanalytical method utilizing solution-phase Candida boidinii formate dehydrogenase (CbFDH) for detecting its oxidized form (NAD+) in human blood plasma samples. The detection mechanism involves the catalytic conversion of NAD+ to NADH, facilitated by CbFDH in the presence of formate. This NADH is then quantified by electrochemical measurements at disposable carbon screen-printed electrodes. The reaction is completed within one minute. The assay exhibits a linear response range from 3.74 µM to 2.00 mM, a sensitivity of 8.98 ± 0.18 µA mM–1, and a limit of detection (3sb/m) of 1.12 µM. It demonstrates selectivity against common interferences found in plasma samples, including glucose, urea, creatinine, guanosine 5’-monophosphate, cytidine 5’-monophosphate, flavin adenine dinucleotide, adenosine 5’-triphosphate, and lactate, with interference levels below 5% relative to the unperturbed NAD+ signal. Recovery studies showed 98.0–104.4% recoveries, with further validation against a colorimetric alcohol dehydrogenase assay confirming accuracy in plasma samples.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"34 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymeric hydrogel integrated paper-based potentiometric ion-sensing device for the determination of sodium ions in human urine

IF 4.2 3区化学

Analyst Pub Date : 2025-01-27 DOI: 10.1039/d4an01505c

Kanyapat Teekayupak, Pattarachaya Preechakasedkit, Natthaya Chuaypen, Thasinas Dissayabutra, Peter A. Lieberzeit, Orawon Chailapakul, Nipapan Ruecha, Daniel Citterio

{"title":"Polymeric hydrogel integrated paper-based potentiometric ion-sensing device for the determination of sodium ions in human urine","authors":"Kanyapat Teekayupak, Pattarachaya Preechakasedkit, Natthaya Chuaypen, Thasinas Dissayabutra, Peter A. Lieberzeit, Orawon Chailapakul, Nipapan Ruecha, Daniel Citterio","doi":"10.1039/d4an01505c","DOIUrl":"https://doi.org/10.1039/d4an01505c","url":null,"abstract":"A paper-based potentiometric sensor integrated with a polymeric hydrogel has been developed for sodium ion (Na+) determination in human urine. The construction of an all-solid-state ion selective electrode (s-ISE) and an all-solid-state reference electrode (s-RE) on a photo paper substrate was achieved using an inkjet printing method. For s-ISE fabrication, carbon nanotubes (CNTs) and gold nanoparticles (AuNPs) were printed on the substrate as a nanocomposite solid contact. A polymeric hydrogel containing lithium acetate (CH3COOLi) was then prepared and used as an intermediate layer to improve the adhesion between the ion selective membrane (ISM) and the AuNP/CNT solid contact, leading to enhanced detection sensitivity. The printed s-RE consisted of a pseudo silver/silver chloride electrode (p-Ag/AgCl) coated with a polymeric hydrogel containing KCl to improve the potential stability of the sensor. Under the optimal conditions, the hydrogel-integrated paper-based potentiometric sensor provided a response toward Na+ over a linear range of 10−7 M to 1 M with a near Nernstian slope of 56.42 ± 0.68 mV per decade. This sensor exhibited fast response, good sensitivity, and reasonable selectivity for Na+ measurement. Furthermore, the developed sensor was effectively applied for the detection of Na+ in urine samples with high accuracy. The presented work can be considered as a good addition to the growing field of potentiometric analytical platforms suitable for large-scale production using inkjet printing technology.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"120 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The aggregation-caused quenching effect and fluorescence enhancement features of ZIF-90 and their feasibility for the detection and imaging of ATP in cells

IF 4.2 3区化学

Analyst Pub Date : 2025-01-27 DOI: 10.1039/d4an01385a

Jing-Hao Fu, Xuan Wang, Qi-He Xia, Zengping Chen, Ping-Fan Shen, Yao Chen, Ruqin Yu

引用次数: 0

Visualization Methods for Loop Mediated Isothermal Amplification (LAMP) Assays.

IF 4.2 3区化学

Analyst Pub Date : 2025-01-24 DOI: 10.1039/d4an01287a

Vinni Thekkudan Novi, Anil Kumar Meher, Abdennour Abbas

{"title":"Visualization Methods for Loop Mediated Isothermal Amplification (LAMP) Assays.","authors":"Vinni Thekkudan Novi, Anil Kumar Meher, Abdennour Abbas","doi":"10.1039/d4an01287a","DOIUrl":"https://doi.org/10.1039/d4an01287a","url":null,"abstract":"Recent advances in nucleic acid (NA) detection techniques have significantly enhanced the diagnosis of diseases caused by a range of pathogens. These NA-based methods that target specific gene sequences for identification offer high specificity. Despite the effectiveness of polymerase chain reaction (PCR), its requirement for sophisticated laboratory settings and expensive equipment restricts its accessibility, particularly in resource-limited settings. As an alternative, isothermal nucleic acid amplification methods are highly sought after due to their rapid, sensitive, and specific detection ability. Among these, loop mediated isothermal amplification (LAMP) stands out due to its simplicity, reliability, and cost-effectiveness. LAMP operates without the need for varied temperature cycles, employing a simple heating block to maintain a constant temperature, thus facilitating onsite rapid testing. In LAMP, the detection step is critical as it shows the outcome of the assay. In order to make the LAMP technique user-friendly and applicable for large scale testing, it is critical to have visual detection where the results can be observed with the naked eye. This review focuses on recent developments of LAMP visualization techniques, including the more common fluorescence, turbidity, and gel electrophoresis methods, as well as innovations in colorimetric techniques applying novel transduction methods such as nanoparticles and digital tools. Additionally, various practical applications of LAMP are discussed.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"2 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of supplementation of macular pigment carotenoids on ocular health: a Raman spectroscopic study of human blood serum of glaucoma patients 补充黄斑色素类胡萝卜素对眼健康的影响：青光眼患者血清的拉曼光谱研究

IF 4.2 3区化学

Analyst Pub Date : 2025-01-23 DOI: 10.1039/d4an01337a

Joy Udensi, James Loughman, Ekaterina Loskutova, Hugh J. Byrne

{"title":"Effects of supplementation of macular pigment carotenoids on ocular health: a Raman spectroscopic study of human blood serum of glaucoma patients","authors":"Joy Udensi, James Loughman, Ekaterina Loskutova, Hugh J. Byrne","doi":"10.1039/d4an01337a","DOIUrl":"https://doi.org/10.1039/d4an01337a","url":null,"abstract":"Carotenoids are known for their antioxidant and vision protection roles, with dietary supplements often promoted for eye health. An initial trial, the European Nutrition in Glaucoma Management (ENIGMA), assessed macular pigment optical density (MPOD) and other ocular parameters before and after supplementing glaucoma patients with macular pigment (MP) carotenoids. The trial confirmed significant improvements in clinical ocular health. Blood, containing all major dietary carotenoids, serves as an efficient medium for in vivo analysis of carotenoids. Raman spectroscopy, an effective analytical tool, was used to measure the impact of supplementation on serum carotenoid levels and their correlation with MPOD and other ocular responses. Serum samples from baseline and 18-month supplemented participants were analysed. An inverse relationship was found between the percentage change in Raman intensity over the supplementation period and baseline Raman serum measurements, indicating greater relative benefits for people with low MPOD/serum carotenoids pre-supplementation. Partial least squares regression (PLSR) was employed to analyse the spectra after pre-processing, and the loadings reflected the carotenoid content and structural profile. MPOD results correlated at all eccentricities, with a coefficient of determination (R2) of 0.62–0.92 and %Root mean squared error of <44%. Structural, functional, and perceptual parameters also showed good correlation with serum Raman measurements. The results support the ENIGMA trial conclusions, and suggest strategies for optimizing patient responses to supplementation based on baseline carotenoid levels. Additionally, Raman spectroscopy of serum carotenoids shows significant potential as a simple and reliable method for investigating macular pigment carotenoids and assessing patient health.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"57 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bis-pyrene polyamine receptor for fast optical detection of ketoprofen: synthesis, characterization and application in all-solid-state fluorescent sensors 用于酮洛芬快速光学检测的双芘多胺受体：合成、表征及其在全固态荧光传感器中的应用

IF 4.2 3区化学

Analyst Pub Date : 2025-01-23 DOI: 10.1039/d4an01469c

Giammarco Maria Romano, Pierangela Di Menna, Andrea Bencini, Yschtar Tecla Simonini Steiner, Massimo Innocenti, Corrado Di Natale, Roberto Paolesse, Larisa Lvova

{"title":"A bis-pyrene polyamine receptor for fast optical detection of ketoprofen: synthesis, characterization and application in all-solid-state fluorescent sensors","authors":"Giammarco Maria Romano, Pierangela Di Menna, Andrea Bencini, Yschtar Tecla Simonini Steiner, Massimo Innocenti, Corrado Di Natale, Roberto Paolesse, Larisa Lvova","doi":"10.1039/d4an01469c","DOIUrl":"https://doi.org/10.1039/d4an01469c","url":null,"abstract":"Here we report on a polyamine receptor L1 bearing pyrene fluorogenic groups for the optical assessment of non-opioid analgesic drug ketoprofen, KP. L1, composed of a diethylenetriamine moiety linked at its extremities to the 1 position of two pyrene units via methylene linkers, gives an emission at 460 nm in water/ethanol 1:1 (v:v) mixture at pH 7, likely due to the excimer formation between the two aromatic groups.. In the presence of KP a salt bridging interaction between the carboxylate group of the analyte and the central ammonium group of L1 induces a redistribution of the acidic protons in the polyamine chain causing a marked increase of the emission. This optical signal was used to detect KP in aqueous media. Inspired by this observation, the properties of all-solid-state optodes with plasticized PVC membranes doped with L1 and deposited on the appropriate solid support material were further tested. The best membrane contained 1wt% of fully protonated L1, plasticized with DOS and doped with 3 equiv. of TDMACl anion-exchanger. It was able to detect KP in 2μM–0.1mM range with a low influence of interfering ions and applied for KP assessment in OkiTask with RSD of 2.1% and recovery of 102%. Moreover, the possibility to low down the KP DL to 0.84 μM (0.21mg/L) through the application of L1 based fluorescent sensor array and chemometrics was shown.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"33 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyst最新文献