IF 4.2 3区化学

Analyst Pub Date : 2025-06-17 DOI: 10.1039/d5an00454c

Xiaohan Liu, Shijiao Fu, Jialong Zhao, Chenjie Gu, Danting Yang, Tao Jiang

{"title":"Label-free SERS detection of foodborne pathogens based on a flexible PMMA-BP@MoS2 binary substrate","authors":"Xiaohan Liu, Shijiao Fu, Jialong Zhao, Chenjie Gu, Danting Yang, Tao Jiang","doi":"10.1039/d5an00454c","DOIUrl":"https://doi.org/10.1039/d5an00454c","url":null,"abstract":"Foodborne pathogenic bacteria, acting as direct or indirect contaminants of food and water sources, have emerged as a significant global food safety issue. Thus, the advancement of rapid, sensitive, and precise methods for detecting foodborne pathogens is of paramount importance for effective food safety monitoring. In response to this critical need, we have successfully prepared a flexible PMMA-BP@MoS2-based surface-enhanced Raman scattering (SERS) substrate through a two-step strategy involving hydrothermal synthesis and spin coating. This novel substrate enables label-free and highly sensitive detection of foodborne pathogens. The synergistic chemical enhancement effect of BP@MoS2 on target molecules, coupled with the highly efficient molecular enrichment capability endowed by PMMA integration, endows the proposed binary substrate with remarkable SERS enhancement properties and stability. These characteristics significantly reduce the detection limit of model molecules to as low as 6.67 × 10−7 M. Furthermore, trace levels of Escherichia coli in raw meat samples were effectively detected using the PMMA-BP@MoS2 flexible substrate, achieving a wide linear detection range from 6.46 × 104 to 6.46 × 108 CFU mL−1. The flexible PMMA-BP@MoS2 SERS substrate emerged as a promising solution for nondestructive and highly sensitive bacterial detection, paving the way for clinical diagnostics of diseases caused by foodborne pathogens and offering a valuable tool to address food safety challenges.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"14 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological functions and detection strategies of magnesium ions: from basic necessity to precise analysis 镁离子的生物学功能和检测策略：从基本需求到精确分析

IF 4.2 3区化学

Analyst Pub Date : 2025-06-17 DOI: 10.1039/d5an00425j

Tianwei Liu, Lan Wang, Siying Pei, Shou Yang, Jiayi Wu, Wei Liu, Qiong Wu

{"title":"Biological functions and detection strategies of magnesium ions: from basic necessity to precise analysis","authors":"Tianwei Liu, Lan Wang, Siying Pei, Shou Yang, Jiayi Wu, Wei Liu, Qiong Wu","doi":"10.1039/d5an00425j","DOIUrl":"https://doi.org/10.1039/d5an00425j","url":null,"abstract":"Magnesium is a ubiquitous element in the natural environment and plays an indispensable role in various biological processes in living organisms. This review focuses on the diverse functions of magnesium ions (Mg2+) in living cells, particularly emphasizing their role in the immune and cardiovascular systems. We discuss how Mg2+ regulates key cellular processes, including enzyme catalysis, cellular signaling and nucleic acid structural stabilization, and explore the adverse effects of magnesium imbalance, which has been associated with numerous diseases ranging from cardiovascular to neurological disorders. Given these critical functions, accurate measurement of Mg2+ is essential. Thus, we also summarize a variety of analytical techniques for Mg2+, ranging from traditional methods like atomic absorption spectrometry and electrochemical sensors to emerging approaches, such as fluorescent probe method and X-ray fluorescence strategy. Bringing together recent advances in Mg2+ detection with a deeper understanding of its biological role, this review aims to promote the systematic integration of Mg2+ research in biomedical and clinical practice, in particular in the field of disease diagnosis and treatment","PeriodicalId":63,"journal":{"name":"Analyst","volume":"10 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroanalysis of Meat Discoloration: Beef Sarcoplasm Extract with Storage Time 肉变色的电分析：牛肉浆萃取物与储存时间

IF 4.2 3区化学

Analyst Pub Date : 2025-06-17 DOI: 10.1039/d5an00301f

Silan Bhandari, Sachinthani A. Devage, Rishav Kumar, Ranjith Ramanathan, Sadagopan Krishnan

{"title":"Electroanalysis of Meat Discoloration: Beef Sarcoplasm Extract with Storage Time","authors":"Silan Bhandari, Sachinthani A. Devage, Rishav Kumar, Ranjith Ramanathan, Sadagopan Krishnan","doi":"10.1039/d5an00301f","DOIUrl":"https://doi.org/10.1039/d5an00301f","url":null,"abstract":"Electrochemical techniques offer a promising approach to monitor redox-active biomolecules involved in meat discoloration. Building on our previous identification of characteristic redox peaks in beef extracts associated with myoglobin oxidation (J. Am Chem. Soc., 2024, 146, 30728–30732, DOI: 10.1021/jacs.4c09375), we present an extended investigation into electrochemical changes over longer meat storage time. Square wave voltammetry revealed frequency-dependent peak potential shifts over 9 days, indicative of a quasi-reversible electron transfer process. Spiking experiments with purified myoglobin in the meat extract confirmed its predominant contribution to the observed voltammetric signals. After 9 days of storage, peak currents at −0.26 V (redox-active myoglobin heme center) and +0.38 V (overall oxidation of meat components) increased by 68.9% and 270.3%, respectively, compared to relative changes of 37.3% and 61.7% in a shorter-duration study. These electrochemical findings showed a strong correlation with spectrophotometrically determined metmyoglobin accumulation (r = 0.98, P < 0.001). The results provide insight into redox transformations during meat spoilage and contribute to the development of electrochemical tools for meat quality evaluation.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"43 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation and identification of commensal and pathogenic oral bacteria at strain level using ATR-FTIR spectroscopy ATR-FTIR光谱在菌株水平上鉴别口腔共生菌和致病菌

IF 4.2 3区化学

Analyst Pub Date : 2025-06-17 DOI: 10.1039/d5an00165j

Katharina Anna Frings, Rumjhum Mukherjee, Vivien Schulze, Nils Heine, Nicolas Debener, Janina Bahnemann, Szymon Piotr Szafrański, Meike Stiesch, Katharina Doll-Nikutta, Maria Leilani Torres-Mapa, Alexander Heisterkamp

{"title":"Differentiation and identification of commensal and pathogenic oral bacteria at strain level using ATR-FTIR spectroscopy","authors":"Katharina Anna Frings, Rumjhum Mukherjee, Vivien Schulze, Nils Heine, Nicolas Debener, Janina Bahnemann, Szymon Piotr Szafrański, Meike Stiesch, Katharina Doll-Nikutta, Maria Leilani Torres-Mapa, Alexander Heisterkamp","doi":"10.1039/d5an00165j","DOIUrl":"https://doi.org/10.1039/d5an00165j","url":null,"abstract":"The correct identification of different bacteria is a critical task in clinical applications and basic research especially in the oral cavity which has a complex bacterial community. Complementary to a variety of phenotyping and genotyping methods, we propose FTIR spectroscopy as a fast and non-destructive technique for accurate bacterial identification. This technique can be used to investigate the chemical makeup of a given sample and also allows for bacterial classification at strain level. In this work, we investigate the ability of ATR-FTIR spectroscopy to identify different oral bacteria from known laboratory stains as well as strains from patient-derived samples. Using this technique, six measured species could be classified with high accuracy (> 97 %) using chemometric models. Furthermore, the model which was only trained with laboratory strains could still correctly identify the patient-derived strains at the genus level. These results open the possibility of constructing a simplified tailored classification model based only on a target species and few other representative species, while still being able to distinguish the target species from a much larger number of other bacterial species for application to oral microbial communities.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"92 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144304606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in field effect transistor biosensors for drug screening applications 用于药物筛选的场效应晶体管生物传感器的最新进展

IF 4.2 3区化学

Analyst Pub Date : 2025-06-16 DOI: 10.1039/d5an00206k

Lemeng Chao, Ying Liang, Xiao Hu

{"title":"Recent advances in field effect transistor biosensors for drug screening applications","authors":"Lemeng Chao, Ying Liang, Xiao Hu","doi":"10.1039/d5an00206k","DOIUrl":"https://doi.org/10.1039/d5an00206k","url":null,"abstract":"Clinical drug screening plays a crucial role in disease treatment, and in understanding the impact of drug monitoring on cellular level biochemical reactions. Field effect transistor (FET) biosensors have emerged as powerful analytical tools integrating multiple disciplines in biomedical research. FET biosensors are pivotal in the biosensor field due to their specificity, high sensitivity, low cost, label-free, real-time detection, and integration abilities. Currently, FET biosensors are fabricated in various configurations, including top-gate, back-gate, dual-gate, solution-gate and extended-gate types. This article reviews the design and applications of FET biosensors in drug screening. First, it introduces the classification, structure mechanism and immobilization methods of bio-probes. Next, it discusses the methods and effects of using FET biosensor platforms to screen cancer drugs, anti-infective drugs, inhibitor drugs, anti-histamines, psychotropic drugs, cardiovascular disease drugs, and other drug categories. The findings suggest that FET biosensors have the potential to provide robust evidence, improving the success rate of new drug development and shortening the drug development cycle.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"11 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144296221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold Disk Microelectrode Coupled Label-free Electrochemical Aptasensor for Dopamine Assay 金圆盘微电极偶联无标记电化学感应传感器用于多巴胺测定

IF 4.2 3区化学

Analyst Pub Date : 2025-06-14 DOI: 10.1039/d5an00511f

Sunying Yang, Jiaqi Cai, Liu Su, Jingxiao Huo, Huadong Xu, Yuhao Gu, Changhe Wang, Hetong Qi, Honglan Qi

{"title":"Gold Disk Microelectrode Coupled Label-free Electrochemical Aptasensor for Dopamine Assay","authors":"Sunying Yang, Jiaqi Cai, Liu Su, Jingxiao Huo, Huadong Xu, Yuhao Gu, Changhe Wang, Hetong Qi, Honglan Qi","doi":"10.1039/d5an00511f","DOIUrl":"https://doi.org/10.1039/d5an00511f","url":null,"abstract":"We fabricate gold disk microelectrode (Au DME) and develop a label-free electrochemical aptasensor for highly sensitive and selective detection of dopamine (DA) in a brain slice by using anti-DA specific aptamer as molecular recognition element and DA oxidation signal as analytical signal. The Au DME with a disk-shaped geometry and a radius in range from 1.25 to 4 μm is fabricated by fine tuning of the size of gold microwire inside a borosilicate capillary with laser-assisted pulling and mechanical polishing method for easily positioning the target object. A label-free electrochemical aptasensor is fabricated by self-assembling an anti-DA specific aptamer on the surface of Au DME with a radius of 2 μm. The obtained aptasensor can directly detect DA based on the oxidation current of DA as analytical signal, in which the recognition of DA by the anti-DA specific aptamer immobilized on Au DME allow DA close to the electrode surface and facilitate the electrochemical oxidation of DA. Taking advantage of Au DME with a small capacitive current and anti-DA specific aptamer with good binding affinity to DA, the label-free electrochemical aptasensor not only can sensitively detect DA with a wide linear range of 0.5 to 27 μM and a low detection limit of 0.11 μM, but also can selectively detect DA in the presence of other interference neurochemicals. Moreover, the label-free electrochemical aptasensor is successfully applied in the recording of the dynamic increase of DA in a brain slice from mice upon electrical stimulation. This work provides a promising strategy for the preparation of label-free electrochemical aptasensor based on microelectrode with high spatial resolution, sensitivity and selectivity to determinate neurochemicals dynamics in living systems.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"7 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ex-vivo spectroscopic characterisation of the biological activity of pancreatic cyst fluid 胰腺囊肿液生物活性的离体光谱表征

IF 4.2 3区化学

Analyst Pub Date : 2025-06-13 DOI: 10.1039/d5an00230c

Outmane Bouzerda, Laura Kane, Gregory Mellotte, Barbara Ryan, Stephen Maher, Olivier Piot, Aidan D. Meade

{"title":"Ex-vivo spectroscopic characterisation of the biological activity of pancreatic cyst fluid","authors":"Outmane Bouzerda, Laura Kane, Gregory Mellotte, Barbara Ryan, Stephen Maher, Olivier Piot, Aidan D. Meade","doi":"10.1039/d5an00230c","DOIUrl":"https://doi.org/10.1039/d5an00230c","url":null,"abstract":"Pancreatic cystic lesions (PCLs) are fluid-filled sacs often detected incidentally through abdominal imaging. While most PCLs are benign with no risk of progressing to pancreatic cancer (PC), a subset can undergo malignant transformation. Differentiating these benign lesions from those that pose a cancer risk remains challenging as current imaging methods and clinical guidelines lack sufficient specificity. Moreover, understanding the biological mechanisms underlying PCL development and progression is critical for optimizing patient management. Well-established PC cell lines and pancreatic cyst fluid (PCF)—the fluid within PCLs—offer valuable tools for studying how PCLs form and potentially transform into malignancies. Insights gained from such investigations could substantially refine patient risk stratification, helping to avoid unnecessary surveillance or treatment. Vibrational spectroscopy represents a promising adjunct to traditional diagnostic techniques, providing objective analysis of PCF and potential in-vivo applications. In this ex-vivo study, we developed a cell-line-based model coupled with vibrational spectroscopy to distinguish how PC cell lines respond to PCF exposure. Our findings demonstrate a robust assay that, with further refinement, may predict disease trajectory and guide precision medicine strategies for patients with PCLs.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"51 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production process defects of Mixi beverage detected by neutral desorption-extractive electrospray ionization mass spectrometry 中性解吸萃取电喷雾质谱法检测混合饮料生产工艺缺陷

IF 4.2 3区化学

Analyst Pub Date : 2025-06-12 DOI: 10.1039/d5an00299k

Lingling Pan, Manman Qin, Leting Wang, Lu Huang, Huiyu Xing, Ning Wang, Tongtong Yang, Liyun Hu, Rui Su, Huanwen Chen

{"title":"Production process defects of Mixi beverage detected by neutral desorption-extractive electrospray ionization mass spectrometry","authors":"Lingling Pan, Manman Qin, Leting Wang, Lu Huang, Huiyu Xing, Ning Wang, Tongtong Yang, Liyun Hu, Rui Su, Huanwen Chen","doi":"10.1039/d5an00299k","DOIUrl":"https://doi.org/10.1039/d5an00299k","url":null,"abstract":"Process defects in the model production process of Mixi beverage, a typical example of extremely viscous food products have been successfully detected using neutral desorption extractive electrospray ionization mass spectrometry (ND-EESI-MS) without sample pretreatment. We employed statistical analysis to explore the correlation between process defects in the production and metabolite ions in the fingerprints of MiXi samples with diverse qualities, which were acquired using ND-EESI-MS. Abnormal signals of analytes such as the appearance of heavy metal ions or pesticides, as well as changes in beneficial ingredients (eg. D-glucose) observed in the fingerprints could serve as indicators of defects encountered in the production. Meanwhile, we quantitatively analysed the concentrations of heavy metals and pesticide residues in Mixi beverage, and the results showed a strong linear relationship (R2 > 0.99) between heavy metals and pesticide residues in the concentration range of 0.50-200.00 μg L-1, with the limit of detection (LOD) of 0.04-1.44 μg L-1 and the limit of quantification (LOQ) of 0.12-4.36 μg L-1 with RSD of 0.39-3.66%. Furthermore, in contrast to previous methods, the concealed defects occurring in the manufacturing process, including raw material screening, soaking, extracting, etc., can be directly detected by terminal product analysis, reflecting ND-EESI-MS was a promising tool for quality control in viscous beverage and tracing defects in the production process.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"12 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of capsaicinoids via Bi2S3 /rGO/MWCNT-COOH-modified screen-printed electrode coupled with glass bead-assisted ultrasonic extraction Bi2S3 /rGO/ mwcnt - cooh修饰网印电极耦合玻璃珠辅助超声提取快速检测辣椒素

IF 4.2 3区化学

Analyst Pub Date : 2025-06-11 DOI: 10.1039/d5an00440c

Jie Jiang, xuening Wang, Kai Yu, Zhiping Sun, Qianyun Yu, Yang Chen

引用次数: 0

Utilising highly conductive TPU “sticks” for facile and low-cost electroanalysis 利用高导电性TPU“棒”进行简单和低成本的电分析

IF 4.2 3区化学

Analyst Pub Date : 2025-06-11 DOI: 10.1039/d5an00472a

Ana C. M. Oliveira, Elena Bernalte, Robert D. Crapnell, Rodrigo A. A. Muñoz, Craig E. Banks

{"title":"Utilising highly conductive TPU “sticks” for facile and low-cost electroanalysis","authors":"Ana C. M. Oliveira, Elena Bernalte, Robert D. Crapnell, Rodrigo A. A. Muñoz, Craig E. Banks","doi":"10.1039/d5an00472a","DOIUrl":"https://doi.org/10.1039/d5an00472a","url":null,"abstract":"Rapid, on-site analysis of environmental contaminants necessitates the use of cost-effective and straightforward apparatus to encourage widespread adoption and align with the United Nations Sustainable Development Goal 6: Clean Water and Sanitation. In this work, we report the development of highly conductive thermoplastic polyurethane (TPU) filaments that can be easily cut and assembled into “sticks”, functioning as standalone rod-like working electrodes for electrochemical and electroanalytical applications. Using TPU as the base polymer, filaments filled with 35, 40, and 45 wt% carbon black (CB) were fabricated and characterised both physicochemically and electrochemically at different lengths (2.5, 5 and 10 mm). Among these, the 45 wt% CB filaments demonstrated the best electrode performance. Interestingly, due to morphological differences between the cross-section and the outer surface of the filament, extending the length of the filament sticks to 10 mm resulted in significant splitting of electrochemical peaks. Consequently, 5 mm sticks were optimised and employed for the electroanalytical detection of acetaminophen, achieving a linear detection range of 5–800 μM, a detection limit of 1.74 μM, and a quantification limit of 5.70 μM. These sticks were further validated by successfully detecting acetaminophen in tap and river water samples. This work introduces an innovative approach to reducing costs and simplifying on-site electrochemical analysis by using predefined lengths of conductive plastic filaments, which can be conveniently transported and stored on a spool until needed. The simplicity and affordability of this method enhance the commercial viability of electroanalytical sensing technologies.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"220 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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