IF 4.2 3区化学

Analyst Pub Date : 2025-05-06 DOI: 10.1039/d5an00079c

Motoki Inoue, Tatsuya Akiyama, Toshiro Fukami

{"title":"Quantification of drug contents in molded tablet using transmission low-frequency Raman spectroscopy","authors":"Motoki Inoue, Tatsuya Akiyama, Toshiro Fukami","doi":"10.1039/d5an00079c","DOIUrl":"https://doi.org/10.1039/d5an00079c","url":null,"abstract":"Orally disintegrating tablets (ODTs) are useful for elderly and pediatric patients suffering from difficulty in swallowing conventional tablets. Among various ODT preparing processes, wet tableting involves a process of the low-pressure compression of wet granules followed by drying, which is promising for the industrial production of medicines. Currently, the quality of wet molded tablets is analyzed via conventional high-performance liquid chromatography–based content uniformity tests. However, owing to the low compression pressure required the wet tableting process, integrating spectroscopic probes with the tableting process can be a potential technique for real-time quality assurance. This study explores the use of transmission low-frequency Raman spectroscopy as a nondestructive and efficient approach for quantifying active pharmaceutical ingredients (APIs) in molded tablets. Acetaminophen and D-mannitol were used as the model API and excipient, respectively. Tablets were prepared by compressing under 150 and 300 N followed by analysis using the partial least square regression method. The distinct spectral features of acetaminophen and D-mannitol enabled accurate quantification with good linear correlations (R2 = 0.98) and low root mean square prediction errors (1.59 for 150 N; 1.22 for 300 N). The method was effective under compressed forces, highlighting it as a real-time, nondestructive tool for quality control in pharmaceutical manufacturing. This approach aligns with continuous manufacturing and quality by design.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"11 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Potential of 3D-Printed Solvent Activated Electrodes for Salicylic Acid Analysis 探索3d打印溶剂活化电极用于水杨酸分析的潜力

IF 4.2 3区化学

Analyst Pub Date : 2025-05-05 DOI: 10.1039/d5an00195a

Karolina Kwaczyński, Olga Szymaniec, Diana Malgorzata Bobrowska, Lukasz Poltorak

引用次数: 0

Smartphone-based portable device for Ag+ sensing using eco-synthesized carbon dots from Mandarin peel 基于智能手机的便携式柑橘皮生态合成碳点Ag+传感装置

IF 4.2 3区化学

Analyst Pub Date : 2025-05-05 DOI: 10.1039/d5an00314h

Nguyen Ngoc Nghia, Dang Thanh Cong Minh, Tran Dang Khoa, Nguyen Huu Hieu

引用次数: 0

Endo IV - Driven Signal Amplifier for Sensitive Detection of Cancer Biomarker TdT 用于肿瘤生物标志物TdT敏感检测的Endo IV驱动信号放大器

IF 4.2 3区化学

Analyst Pub Date : 2025-05-02 DOI: 10.1039/d5an00203f

Min Lin, Ziying Ye, Wei Dai, Jin Tu, Yutong Li, Jie Yu, Xianjin Xiao, Chun Li, Longjie Li

{"title":"Endo IV - Driven Signal Amplifier for Sensitive Detection of Cancer Biomarker TdT","authors":"Min Lin, Ziying Ye, Wei Dai, Jin Tu, Yutong Li, Jie Yu, Xianjin Xiao, Chun Li, Longjie Li","doi":"10.1039/d5an00203f","DOIUrl":"https://doi.org/10.1039/d5an00203f","url":null,"abstract":"Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase and its function is mainly involved in the development of the human immune system. The activity of TdT is normally suppressed in healthy human cells but is overexpressed in certain diseases, such as leukemia and other malignant blood disorders. Therefore, the detection of TdT activity is crucial for clinical diagnosis and disease progression monitoring. In this study, we developed an Endo IV-driven signal amplifier to assess TdT activity. TdT can recognize primers and add dATP to the 3' hydroxyl end of the primers, forming a polyA tail. Fluorescent probes (polyT) containing AP sites can bind to the polyA tail and the AP site will be hydrolyzed by Endo IV. Then the fluorescent probe is cut and emit fluorescence. Subsequently, the intact fluorescent probe rebinds to the polyA tail, creating a cyclic process for signal amplification. The detection limit for TdT was 1×10-4 U/μL, with a linear range of 1×10-4 to 1×10-1 U/μL. This biosensor expands the current TdT detection toolkit. Its excellent specificity and sensitivity in simulated biological environments provide a powerful detection tool for clinical TdT testing.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"9 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaky Waveguide Biosensors for Label-free Measurement of Human Serum Albumin 无标记测量人血清白蛋白的漏波导生物传感器

IF 4.2 3区化学

Analyst Pub Date : 2025-05-02 DOI: 10.1039/d5an00108k

Anil Kumar Pal, Md Nazmul Hossain, Saverio Brogna, Nicholas Goddard, Ruchi Gupta

{"title":"Leaky Waveguide Biosensors for Label-free Measurement of Human Serum Albumin","authors":"Anil Kumar Pal, Md Nazmul Hossain, Saverio Brogna, Nicholas Goddard, Ruchi Gupta","doi":"10.1039/d5an00108k","DOIUrl":"https://doi.org/10.1039/d5an00108k","url":null,"abstract":"Early diagnosis of diseases such as kidney disease relies on the successful measurement of albumin concentration in urine. We report label-free detection of human serum albumin (HSA) using a leaky waveguide (LW) optical biosensor. The LW reported in this work comprised a few microns-thick mesoporous polyacrylamide hydrogel film deposited on a glass substrate by casting and, for the first time, copolymerized with N-(3-aminopropyl)methacrylamide (APMAA) to provide functional amine groups required to immobilise recognition elements, half-antibody fragments. Furthermore, this is an unprecedented report on the use of a high molecular weight (3700 D) poly(ethylene glycol) diacrylamide in contrast to previously reported low molecular weight bis-acrylamide crosslinker to increase the porosity of waveguide films. Equally, other parameters such as molar ratio of APMAA to acrylamide and total weight of (monomers and crosslinker) to volume ratio were optimised to obtain hydrogel films with pore size and amine groups required to immobilise half-antibody fragments in hydrogel films. Three different strategies for immobilisation of recognition elements; two based on streptavidin biotin interactions and the third based on half fragments of antibody were studied. The third immobilisation strategy resulted in the most reproducible results and hence was used to measure the equilibrium dissociation constant of HSA and its corresponding half-antibody fragments. Using the LW-based label-free optical biosensor, HSA was successfully detected with a limit of detection of 28 ng/mL in buffer and the lowest concentration of HSA measured in this work was 66 ng/mL. This capability of quantitation of HSA by the LW can be built upon to realise a LW biosensor for early detection of diseases including kidney disease.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"43 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on cavity-driven control methods for concentration gradient microdroplets with high precision and stability 高精度、稳定的浓度梯度微滴腔驱动控制方法研究

IF 4.2 3区化学

Analyst Pub Date : 2025-05-02 DOI: 10.1039/d5an00422e

Huimei Lin, Jianhong Dong, Qing Yu, Junsheng Wang

{"title":"Research on cavity-driven control methods for concentration gradient microdroplets with high precision and stability","authors":"Huimei Lin, Jianhong Dong, Qing Yu, Junsheng Wang","doi":"10.1039/d5an00422e","DOIUrl":"https://doi.org/10.1039/d5an00422e","url":null,"abstract":"Microfluidic chip technology, an emerging interdisciplinary field, enables precise control of fluids at micro- and nano-scales and is widely applied in biomedicine, chemical analysis, and drug screening. This study investigates the effect of the parameters of chip geometry and the flow rate of fluid on the performance of the droplet concentration gradient generator using a cave-based microfluidic chip structure through numerical simulation and experimental verification. Finite element simulation software, equipped with laminar flow, phase field, and dilute substance transfer modules, was employed to analyze the impact of geometric parameters (cavity number, cavity radius, contact length between the cavity and the central channel, and cavity interval length) on the concentration gradient curve. Additionally, the effect of the fluid flow rate on the frequency, size, and concentration of the generated microdroplets was explored. Results indicate that the number, radius, and contact length of cavities significantly affect the concentration gradient curve, while the interval length has a minimal impact. Precise control of droplet concentration, generation frequency, and size can be achieved by adjusting the flow rates of the dispersed phase and the continuous phase. This study provides a theoretical basis for the design and optimization of concentration gradient microfluidic chips and promotes the application of concentration gradient microdroplet technology in high-throughput drug screening, biological detection and chemical analysis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"52 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in biosensors based on the electrochemical properties of MXene 基于MXene电化学特性的生物传感器研究进展

IF 4.2 3区化学

Analyst Pub Date : 2025-05-02 DOI: 10.1039/d5an00235d

Luming Pan, Dongtao Zhou, Yuzhen Wang

引用次数: 0

Insights Into The Cellular Lipid Cascade of Prostate Cells Explored Using Infrared Microspectroscopy 利用红外显微光谱学研究前列腺细胞的细胞脂质级联

IF 4.2 3区化学

Analyst Pub Date : 2025-05-01 DOI: 10.1039/d5an00126a

Thomas A Gladwell, Dougal Ferguson, Noel William Clarke, Michael Brown, Peter Gardner

{"title":"Insights Into The Cellular Lipid Cascade of Prostate Cells Explored Using Infrared Microspectroscopy","authors":"Thomas A Gladwell, Dougal Ferguson, Noel William Clarke, Michael Brown, Peter Gardner","doi":"10.1039/d5an00126a","DOIUrl":"https://doi.org/10.1039/d5an00126a","url":null,"abstract":"Background: Although prostate cancer (PCa) is the most diagnosed cancer in men worldwide, there is geographical variance in both incidence and morbidity, with higher levels in developed “Western Diet” countries. In particular the high levels of the omega-6 polyunsaturated fatty acid, arachidonic acid (AA), in Western diets has been shown to promote aggressive PCa in vitro. However the exact mechanism through which AA induces the aggressive phenotype has not been fully characterised. Methods: In this study Fourier transform infrared (FTIR) imaging coupled with fluorescence microscopy (FM), is used to follow AA metabolism in PCa cell lines. This is achieved using partially deuterated AA, with a distinctive C-D stretch seen at 2251 cm–1 providing molecular specificity, coupled with Nile Red Fluorescence imaging. Results: We show that, invasive cell lines PC-3, LNCaP C4-2B and DU145 readily uptake and metabolise AA, producing prostaglandins via the COX-2 pathway. Inhibition of the COX-2 pathway with either NS938 or the omega-3 polyunsaturated fatty acid Docosahexaenoic acid (DHA), reduces the invasive stimulus of AA and blocks its uptake. Conclusion: This demonstrates that FTIR imaging can be utilised to follow metabolomics processes within a PCa model and provide an insight to the molecular pathways underlying the cancer metabolome.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"1 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of an integrated UHPLC-MS/MS and GC-MS method for the simultaneous analysis of three categories of phenolic endocrine disrupting chemicals in surface water UHPLC-MS/MS和GC-MS联合分析地表水中3类酚类内分泌干扰物方法的建立与验证

IF 4.2 3区化学

Analyst Pub Date : 2025-04-29 DOI: 10.1039/d5an00415b

Nan Zhang, Xinyuan Pei, Huiyang Fu, Shiyu Miao, Qingqing Zhu, Zhengbo Dai, Yi Chi, Guohua Zhu, Ligang Hu, Rong Jin, Chunyang Liao

{"title":"Development and validation of an integrated UHPLC-MS/MS and GC-MS method for the simultaneous analysis of three categories of phenolic endocrine disrupting chemicals in surface water","authors":"Nan Zhang, Xinyuan Pei, Huiyang Fu, Shiyu Miao, Qingqing Zhu, Zhengbo Dai, Yi Chi, Guohua Zhu, Ligang Hu, Rong Jin, Chunyang Liao","doi":"10.1039/d5an00415b","DOIUrl":"https://doi.org/10.1039/d5an00415b","url":null,"abstract":"The global concern over emerging contaminants, particularly endocrine-disrupting chemicals (EDCs), has driven the need for advanced detection methods. Phenolic EDCs, such as parabens, bisphenols, and synthetic phenolic antioxidants (SPAs), are widely distributed in the environment and pose risks to reproductive systems and metabolism. With ongoing advancements in research, the development of high-throughput technologies for the simultaneous detection of these phenolic EDCs has become a pressing priority. A sensitive method combining ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) was developed for the simultaneous determination of three typical classes of phenolic EDCs in aquatic environments, including parabens, bisphenols, and SPAs. Solid-phase extraction (SPE) parameters and instrumental conditions were optimized, achieving recoveries of 64.7%–123% and 64.0%–111% for blank spikes and matrix spikes, respectively, with relative standard deviation below 18%. Detection and quantification limits were determined to be 0.228–0.940 ng L−1 and 0.758–3.13 ng L−1, respectively. The method was successfully applied to 23 surface water samples, and 10 EDCs were detected. Notably, bisphenols and SPAs in the Qiantang River displayed a clear increasing trend toward the downstream. SPAs exhibited the highest concentrations, with oxidation by the π-system being the predominant pathway responsible for the transformation to degradants like BHT-Q and BHT-quinol. This method offers reliable detection and quantification of multiple phenolic EDCs in water, providing a valuable tool for environmental monitoring, risk assessment, and regulatory management of EDCs contamination.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"274 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143885386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible hand-made carbon electrode decorated with metronidazole imprinted polymer 用甲硝唑印迹聚合物装饰的柔性手工碳电极

IF 4.2 3区化学

Analyst Pub Date : 2025-04-28 DOI: 10.1039/d5an00146c

Dominik Korol, Kostiantyn Nikiforow, Pawel Borowicz, Piyush Sindhu Sharma, Maciej Cieplak

引用次数: 0

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