IF 4.2 3区化学

Analyst Pub Date : 2025-09-29 DOI: 10.1039/d5an00993f

Liping Lu, kang chunyu

{"title":"Electrochemical Investigation of Intracellular NADH: PASHs Effect on Mitochondrial Function","authors":"Liping Lu, kang chunyu","doi":"10.1039/d5an00993f","DOIUrl":"https://doi.org/10.1039/d5an00993f","url":null,"abstract":"Nicotinamide adenine dinucleotide (NADH) is a crucial biomolecule that serves as an essential cofactor in numerous biocatalytic reactions and a pivotal regulator of mitochondrial function and cellular physiology. This study developed a SECM platform utilizing a double-mediator system for the real-time, in situ, and non-invasive detection of intracellular NADH at the single living cell level. The system employs 1-methoxy-5-methylphenazinium methyl sulfate (mPMS), which permeates the cell membrane to oxidize intracellular NADH, and ferricyanide ([Fe(CN)₆]³⁻), which shuttles electrons from the reduced mPMS to the SECM tip. This methodology was applied to investigate mitochondrial impairment in human hepatoma (HepG2) cells induced by polycyclic aromatic sulfur heterocycles (PASHs). The metabolic response was corroborated by analyzing poly(ADP-ribose) polymerase (PARP) activity, adenosine triphosphate (ATP) content, and mitochondrial membrane potential (MMP). Results demonstrated that exposure to dibenzothiophene (DBT) activated PARP, leading to severe depletion of NADH and ATP and a significant decline in MMP, thereby confirming comprehensive mitochondrial dysfunction. Comparative analysis with its non-sulfur analog fluorene (Flu) revealed that the sulfur atom in DBT's structure is a critical determinant of its enhanced cytotoxicity. This work establishes a reliable and innovative analytical approach for assessing the subcellular metabolic effects of environmental pollutants, providing a new platform for in-depth cytotoxicity mechanistic studies.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"21 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Portable proton transfer reaction ion trap mass spectrometer with selectable buffer gas. 便携式质子转移反应离子阱质谱仪，可选择缓冲气体。

IF 4.2 3区化学

Analyst Pub Date : 2025-09-29 DOI: 10.1039/d5an00703h

Jun Wang,La Chen,Chengjie Jiang,Gangqiang Li,Luhong Wen

{"title":"Portable proton transfer reaction ion trap mass spectrometer with selectable buffer gas.","authors":"Jun Wang,La Chen,Chengjie Jiang,Gangqiang Li,Luhong Wen","doi":"10.1039/d5an00703h","DOIUrl":"https://doi.org/10.1039/d5an00703h","url":null,"abstract":"Proton transfer reaction mass spectrometry (PTR-MS) is a rapid and highly sensitive technique for the detection of volatile organic compounds and has been increasingly used in the fields of environmental monitoring, medicinal diagnosis and food industry. The large size and heavy weight of conventional PTR-MS devices limit their on-site application for real-time and rapid analysis. In this work, a miniaturized PTR-MS instrument with a total weight of 25 kg was developed on the basis of a portable ion trap mass spectrometer, which was characterized by the use of a small hollow-cathode discharge for hydroxonium ion generation and a compact ion funnel as the drift tube. For this instrument, mass resolution, ion isolation and fragmentation efficiencies, and sensitivity have been investigated in detail with helium and nitrogen as buffer gases. Compared with nitrogen buffer gas, when helium buffer gas was used, the mass resolution of protonated acetone ions doubled, whereas the ion fragmentation efficiency could be increased by a factor of 17. With helium buffer gas, the limits of detection for acetone, benzene, and toluene were found to be 1 ppbv, 0.5 ppbv, and 0.1 ppbv, respectively, and linear calibration curves for these compounds were established across concentration ranges spanning at least two orders. Under the optimal vacuum pressures of 1.5 mbar and 2.5 mbar, the gas consumption rates for helium and nitrogen were 81 mL min-1 and 60 mL min-1, respectively. In addition, the sample injection flow rate exerted a pronounced influence on the detection sensitivity and response time of the system.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"3 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-responsive interpenetrating network hydrogel coated silica used for multi-mode stationary phases. 用于多模固定相的温度响应互穿网络水凝胶包覆二氧化硅。

IF 4.2 3区化学

Analyst Pub Date : 2025-09-29 DOI: 10.1039/d5an00883b

Shuning Li,Liwei Gu,Xiaojing Liang,Shuai Wang,Licheng Wang

{"title":"Temperature-responsive interpenetrating network hydrogel coated silica used for multi-mode stationary phases.","authors":"Shuning Li,Liwei Gu,Xiaojing Liang,Shuai Wang,Licheng Wang","doi":"10.1039/d5an00883b","DOIUrl":"https://doi.org/10.1039/d5an00883b","url":null,"abstract":"In this work, a novel type of liquid chromatography stationary phase (Sil-NH2@CMC/PNIPAM) with a multi-mode separation capability was obtained by coating a hydrogel onto the surface of amino silica (Sil-NH2). The hydrogel comprises an interpenetrating network of carboxymethyl chitosan (CMC) and poly(N-isopropyl acrylamide) (PNIPAM). The modification process was successfully verified using Fourier Transform Infrared (FI-IR) spectroscopy, Scanning Electron Microscopy (SEM) and Thermogravimetric Analysis (TGA). This stationary phase effectively enables the separation and analysis of various compounds in three distinct modes: hydrophilic interaction chromatography (HILIC), reversed-phase liquid chromatography (RPLC), and ion exchange chromatography (IEC). Notably, PNIPAM exhibits a pronounced temperature-responsive characteristic in the stationary phase. Our experimental results suggest that this responsiveness is mainly attributed to the weakening of hydrogen bonds within PNIPAM as the temperature rises. Such weakening prompts the contraction of molecular chains, making the interpenetrating network more compact. As a result, there is a significant change in the retention behavior of the analyte. Additionally, the Sil-NH2@CMC/PNIPAM column has demonstrated its efficacy in the separation and analysis of the ethanol extract from Codonopsis pilosula. Overall, this temperature-responsive and multi-mode stationary phase offers promising avenues for future innovations in High-performance Liquid Chromatography (HPLC).","PeriodicalId":63,"journal":{"name":"Analyst","volume":"195 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Pressure ESI with High-Temperature Pre- and Post-ESI Heating for High-Throughput Analysis of Hydrothermal and Gas-Phase Thermal Effects on Analytes 高压ESI与高温ESI前后加热用于高通量分析水热和气相热效应

IF 4.2 3区化学

Analyst Pub Date : 2025-09-26 DOI: 10.1039/d5an00599j

XIANG ZHANG, Zhihua Ying, Lee Chuin Chen

{"title":"High-Pressure ESI with High-Temperature Pre- and Post-ESI Heating for High-Throughput Analysis of Hydrothermal and Gas-Phase Thermal Effects on Analytes","authors":"XIANG ZHANG, Zhihua Ying, Lee Chuin Chen","doi":"10.1039/d5an00599j","DOIUrl":"https://doi.org/10.1039/d5an00599j","url":null,"abstract":"We report a high-pressure electrospray ionization (ESI) source that incorporates a heated liquid transfer capillary, which functions as an online hydrothermal reactor, and a heated ion transport capillary for high-throughput analysis of heating effects on analytes and on-demand thermal dissociation. Both capillaries are resistively heated to elevated temperatures. The heated liquid transfer capillary is connected to the high-pressure ESI via a flow restrictor, and the back pressure provided by the flow restrictor, combined with the high-pressure operation of the ESI source, allows the liquid to be heated to 300 ℃ without boiling before being cooled to < 150 ℃ at the ESI emitter. The generated ions and charged droplets then pass through the ion transport capillary, which can be held at 20 ~ 400 ℃ before being released into the atmospheric pressure ambient for subsequent sampling by the mass spectrometer. The system has been applied to the hydrothermal acceleration of Asp-selective cleavage in ubiquitin, Pro-Pro bond-selective cleavage in bradykinin, and the formation of dipeptides of Gly-Gly and Ala-Ala from glycine and alanine. Post-ESI heating of the charged droplets and ions did not produce similar hydrothermal reactions; instead, it resulted in gas-phase thermal dissociation that differs from liquid-phase activation. The combination of liquid-phase and gas-phase activations can be used to tune the level of fragmentation and increase the abundance of fragments for tandem mass spectrometry, providing a potential tool for top-down proteomics.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"27 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell western blotting of cytoplasmic cytokeratin 8 proteoforms. 细胞质细胞角蛋白8蛋白形态的单细胞免疫印迹。

IF 4.2 3区化学

Analyst Pub Date : 2025-09-26 DOI: 10.1039/d5an00776c

Anna Fomitcheva Khartchenko,Trinh Lam,Amy E Herr

{"title":"Single-cell western blotting of cytoplasmic cytokeratin 8 proteoforms.","authors":"Anna Fomitcheva Khartchenko,Trinh Lam,Amy E Herr","doi":"10.1039/d5an00776c","DOIUrl":"https://doi.org/10.1039/d5an00776c","url":null,"abstract":"Differential detergent fractionation (DDF) enables compartment-specific lysis, offering a strategy to analyze cytoplasmic proteins while preserving the nucleus for downstream assays. However, while this method facilitates sub-cellular resolution, current single-cell approaches using DDF remain limited in their ability to identify proteoforms without compromising nuclear integrity. This limitation is especially pronounced in proteins where their proteoforms present diverse biological functions such as cytokeratin 8 (CK8), a structural protein implicated in several disease states. Here, we present a single-cell western blot (scWB) integrated with DDF to selectively solubilize and separate CK8 proteoforms while preserving nuclear integrity. To evaluate assay applicability and nuclear stability, we profiled CK8 across breast cancer cell lines (MCF7, SKBR3, and MDA-MB-231), confirming proteoform detection only in MCF7 and preservation of nuclear content across all lines. We report on assay development, including screening a panel of lysis buffers based on nonionic detergents, and electrophoresis conditions to achieve a separation resolution between two proteoforms of up to 0.94, while preserving an intact nucleus. The cytoplasm-specific lysis (DDF buffer) yielded detectable proteoforms in 14.3% of solubilized single cells, comparable to 10.3% with whole-cell lysis (RIPA buffer). Our approach allows for tailored solubilization, achieving reliable proteoform detection and nuclear retention across different cell types. Proteoform profiling at the single-cell level forms a basis for the exploration of the role of specific CK8 molecular forms in cellular processes.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"42 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hybrid NGO-PSO optimized random forest combined with multi-energy LIBS for enhanced accurate classification of tea. 混合NGO-PSO优化的随机森林结合多能LIBS提高茶叶的准确分类。

IF 4.2 3区化学

Analyst Pub Date : 2025-09-26 DOI: 10.1039/d5an00559k

Junjie Ma,Xiaojian Hao,Rui Jia,Biming Mo,Junjie Chen,Shuaijun Li,Hongkai Wei,Yaozhong Tian,Songtao Gao

{"title":"Hybrid NGO-PSO optimized random forest combined with multi-energy LIBS for enhanced accurate classification of tea.","authors":"Junjie Ma,Xiaojian Hao,Rui Jia,Biming Mo,Junjie Chen,Shuaijun Li,Hongkai Wei,Yaozhong Tian,Songtao Gao","doi":"10.1039/d5an00559k","DOIUrl":"https://doi.org/10.1039/d5an00559k","url":null,"abstract":"Accurately measuring and analyzing the chemical composition and spectral characteristics of tea is of great significance for improving the sorting accuracy and preventing tea adulteration and variety misjudgment. We propose a tea identification and classification system that combines multiple settings of laser-induced breakdown spectroscopy (LIBS) and a Random Forest model that combines the Northern Eagle and Particle Swarm Optimization algorithm (NGO-PSO-RF), which provides a new idea for efficient and accurate classification and recognition of tea. The experiment increased the spectral difference between similar samples by measuring the spectral data of eight tea samples at three different energies. Principal Component Analysis (PCA) was performed on the spectral data at each energy, and the first three principal components were extracted to construct the fusion feature set. PCA dimension reduction was used again to obtain the core feature vector. The original complex multi-energy spectral data were simplified by performing two analyses. Finally, the fusion spectral data were analyzed using the NGO-PSO-RF model, and the classification accuracy of the test set was 99.22%, which was 3.13 percentage points higher than the average classification accuracy (96.09%) at a single energy, and the core indicators, such as the recall rate (+3.11%) and F1 value (+3.19%), were synchronously optimized. In addition, compared with LSTM, SVM, RF and PSO-RF, the classification accuracy of the NGO-PSO-RF model improved by 6.77%, 5.47%, 5.34% and 3.52% respectively. This method provides an innovative and efficient method with high universality and robustness for material classification and provides important technical support and reliable detection prospects for food production safety and chemical analysis.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"69 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145140514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances and Applications of Organoid-on-a-Chip in Disease Modeling 类器官芯片在疾病建模中的应用进展

IF 4.2 3区化学

Analyst Pub Date : 2025-09-25 DOI: 10.1039/d5an00514k

Yujia Yang, Jinhui Sheng, Fengqiu Sheng, Xiangyang Liang, Cuixia Ma, Ruixue Chen, Shengkun Wang, Xuesong Qian

引用次数: 0

Silica Surface Modification via Diazotization of p-Phenylenediamine: A Stationary Phase for HPLC 用对苯二胺重氮化法修饰二氧化硅表面：高效液相色谱固定相

IF 4.2 3区化学

Analyst Pub Date : 2025-09-25 DOI: 10.1039/d5an00869g

Joseph R Ezzo, Brandon L Salazar, Raúl J Díaz-Santiago, Luis A Colon

{"title":"Silica Surface Modification via Diazotization of p-Phenylenediamine: A Stationary Phase for HPLC","authors":"Joseph R Ezzo, Brandon L Salazar, Raúl J Díaz-Santiago, Luis A Colon","doi":"10.1039/d5an00869g","DOIUrl":"https://doi.org/10.1039/d5an00869g","url":null,"abstract":"A phenylenediamine-derived layer was grafted onto superficially porous silica particles through the diazotization of p-phenylenediamine (p-PDA), circumventing traditional silica silanization reactions. The phenylene-type layer was characterized using diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), X-ray photoelectron spectroscopy (XPS), and elemental analysis. XPS revealed a surface layer containing approximately one nitrogen atom for every three aryl rings. In addition, the layer on silica included azo-linkages of aryl groups and amine functionalities, with one amine group per four to five benzene rings. Elemental analysis indicated that surface coverage of the aryl groups could be modulated by adjusting the reaction conditions, thereby enabling control over surface loading while reducing potential pore obstruction. A p-PDA-derived layer having 2-4 rings/nm2 proved effective as a stationary phase for high-performance liquid chromatography (HPLC) and exhibited excellent hydrolytic stability under relatively harsh acidic conditions (0.5% TFA at 80 °C). Furthermore, beyond small molecules, the new phase showed promise for peptide separation.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"17 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visual enumeration of circRNA in serum using a biserrate DNA-modified AuNP SPR probe 使用双酸dna修饰的AuNP SPR探针对血清中环状rna的目视计数

IF 4.2 3区化学

Analyst Pub Date : 2025-09-25 DOI: 10.1039/d5an00875a

Yuanzhao Shen, Ran Wan, Xin Zhou

引用次数: 0

Prediction of clinical outcomes of ST-elevated myocardial infarction patients using atmospheric solids analysis probe mass spectrometry and machine learning 使用大气固体分析探针质谱和机器学习预测st段升高的心肌梗死患者的临床结果

IF 4.2 3区化学

Analyst Pub Date : 2025-09-24 DOI: 10.1039/d5an00565e

Annabel Selina Jessica Eardley-Brunt, Thomas Mills, Rafail Kontronias, Giovanni Luigi de Maria, Keith Channon, The Oxford Acute Myocardial Infarction Study, Claire Vallance

{"title":"Prediction of clinical outcomes of ST-elevated myocardial infarction patients using atmospheric solids analysis probe mass spectrometry and machine learning","authors":"Annabel Selina Jessica Eardley-Brunt, Thomas Mills, Rafail Kontronias, Giovanni Luigi de Maria, Keith Channon, The Oxford Acute Myocardial Infarction Study, Claire Vallance","doi":"10.1039/d5an00565e","DOIUrl":"https://doi.org/10.1039/d5an00565e","url":null,"abstract":"Introduction: Analysis of small molecule metabolites found in blood plasma of patients undergoing treatment for STEMI has the potential to be used as a clinical diagnostic and prognostic tool, capable of predicting disease progression, risk of negative outcomes, and response to treatment. Methods: Rapid mass spectrometry has been used to measure the metabolite profiles of coronary aspirate blood plasma from 288 STEMI patients enrolled in the Oxford Acute Myocardial Infarction (OxAMI) study. Supervised machine learning applied to the mass spectra was used to stratify patients based on clinically relevant variables related to health and treatment response. Results: In this small proof-of-concept study, patient mortality and microvascular obstruction (MVO) were predicted with over 80% accuracy; heart failure diagnosis, ischemic time, peak troponin, and thrombus score were predicted with over 76% accuracy, and creatinine and index of microcirculatory resistance (IMR) were predicted with over 70% accuracy. Using feature-reduction methods, we were able to identify key mass-to-charge (<em>m/z</em>) peaks in the mass spectra that correlated with the assignment to particular patent groups. These may potentially be used in the future as mass spectrometric biomarkers in the development of a diagnostic and prognostic test for STEMI risk.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"73 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145127944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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