酶促合成稳定同位素标记的SAM用于基质辅助激光解吸/电离质谱法筛选hMAT2A抑制剂

IF 3.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst Pub Date : 2025-09-24 DOI:10.1039/d5an00705d

Lingna Zheng, Haiyu Lu, Qing Yang, Yixian Liu, Chunjing Chen, Qianzhen Li, Xiang Gao

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引用次数: 0

摘要

建立了基质辅助激光解吸/电离质谱法（MALDI-TOF-MS）筛选人MAT2A （hMAT2A）抑制剂的高通量方法。以L-Met-D3和ATP为底物，通过hMAT2A酶促合成稳定同位素氘标记s -腺苷蛋氨酸（SAM-D3），作为酶活性测定的内标物，可显著提高高通量筛选的定量准确性。

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Enzymatic synthesis of stable isotope-labeled SAM for screening hMAT2A inhibitors by using matrix-assisted laser desorption/ionization mass spectrometry

A high throughput method was developed for screening human MAT2A (hMAT2A) inhibitors by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS). The stable isotope deuterium-labeled S-adenosylmethionine (SAM-D3) was enzymatically synthesized by hMAT2A with using L-Met-D3 and ATP as substrates and used as the internal standard for enzyme activity assay, which could significantly improve the quantitative accuracy of high throughput screening.

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