Curcumin – A natural colorant-based colorimetric pH indicator for molecular diagnostics

Abstract

Loop-mediated isothermal amplification (LAMP) provides highly selective and sensitive DNA amplification and generates hydrogen ions as a by-product under weakly buffered conditions, causing the solutions’ pH to decrease from the initial basic to an acidic environment. This distinctive feature allows the color of the amplified DNA solution to change readily when suitable pH indicators are employed. In this study, curcumin, a biodegradable, non-toxic, and natural colorant, was used as a pH indicator to visually identify LAMP-amplified Staphylococcus aureus (S. aureus) and Streptococcus pneumoniae (S. pneumoniae). Curcumin (10 mM) displayed a unique color difference between negative (red) and positive (yellow) samples, and the detection process was completed within 30 s, demonstrating the effectiveness of using curcumin for on-site diagnostics. Under optimum conditions, curcumin enabled S. aureus and S. pneumoniae detection as low as 10 fg/µL and 1 pg/µL, respectively, due to its unique halochromic properties. Owing to its adaptability, ease of use, and rapid visual detection, the introduced colorimetric pH-based LAMP method can be employed as a practical alternative to conventional colorimetry for infectious pathogen identification in both laboratory and field settings.