The Royal Society of Chemistry最新文献

{"title":"A green and cascade synthesis of functionalized 2-aminothiophenes from ketene dithioacetals.","authors":"Mohit Chahal, Ranjay Shaw, Pratik Yadav, Vishnu Bhardwaj, Ismail Althagafi, Ramendra Pratap","doi":"10.1039/d5ob01121c","DOIUrl":"https://doi.org/10.1039/d5ob01121c","url":null,"abstract":"<p><p>We have reported the synthesis of pharmacologically valuable tetrasubstituted 2-aminothiophenes through a multi-component reaction using ketene dithioacetals, secondary amines, and thioglycolates in the presence of a catalytic amount of triethylamine under aqueous conditions. Various substrates, including symmetrical and asymmetrical ketene dithioacetals, were explored, demonstrating versatility in product formation. Notably, reactions involving ethyl 2-cyano-3,3-bis(methylthio)acrylate selectively produced single products. The mechanistic proposal elucidates the steric effects of secondary amines on reaction pathways, which facilitate the unique selectivity of intermediates and hence product selectivity. Additionally, the synthesis of piperidone-bearing thiophenes was successfully achieved, underscoring the methodology's potential for generating biologically relevant compounds. The synthesized compounds were characterized by spectroscopic techniques, and the structure of four compounds (3a, 5a, 5b, and 7c) has been confirmed by single-crystal X-ray diffraction. This research contributes to the development of efficient synthetic strategies for 2-aminothiophenes, offering pathways for future drug discovery and development.</p>","PeriodicalId":96,"journal":{"name":"Organic & Biomolecular Chemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress in antibacterial application based on metal porous materials.","authors":"Bingbing Chen, Jing Hou, Baiyang Liu, Weiyang Gong, Xinran Yang, Zheming Yang, Zengchao Guo","doi":"10.1039/d5cc04727g","DOIUrl":"https://doi.org/10.1039/d5cc04727g","url":null,"abstract":"<p><p>With the escalating issue of bacterial resistance, traditional antibiotic therapies are facing severe challenges, making the development of novel and efficient antibacterial materials a prominent research focus. Metal porous materials, owing to their unique three-dimensional interconnected pore structures, high specific surface areas, and tunable physicochemical properties, have demonstrated significant potential in the field of antibacterial applications. These materials can achieve synergistic antibacterial effects through multiple mechanisms, including the sustained release of metal ions, photothermal and photodynamic therapies (PTT and PDT), as well as contact-mediated sterilization. Such mechanisms contribute to enhanced antibacterial performance while reducing the risk of bacterial resistance. Metal porous materials are regarded as promising candidates for the next generation of efficient and safe antibacterial platforms, with broad applicability in medical and bioengineering fields. This review summarizes recent advances in the research of metal porous materials, focusing on their synthesis methods, antibacterial mechanisms, and practical applications. The synthesis strategies discussed include template methods, electrochemical deposition, solvothermal synthesis, melt foaming, and microwave sintering. In addition, current challenges such as large-scale fabrication, precise regulation of metal ion release, and biosafety concerns are critically discussed. The paper also outlines the prospects of metal porous materials in antibacterial medical applications.</p>","PeriodicalId":67,"journal":{"name":"Chemical Communications","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial UPR is required for resveratrol mediated anti-bacterial immunity.","authors":"Yan Zhang, Xiaocong Li, Rui Zhao, Wanting Hu, Xin Xiao, Yi Xiao, Fang Liu","doi":"10.1039/d5fo03539b","DOIUrl":"https://doi.org/10.1039/d5fo03539b","url":null,"abstract":"<p><p>The mitochondrial unfolded protein response (UPR^mt), a crucial mechanism for maintaining mitochondrial homeostasis, has recently been shown to regulate innate immune responses. Resveratrol, a natural polyphenolic compound abundant in grapes and peanuts, exhibits diverse biological activities including anti-inflammatory, antioxidant, anti-aging, and anticancer effects. However, whether resveratrol modulates innate immunity and its underlying mechanisms remain unclear. In this study, we demonstrated that resveratrol significantly enhanced resistance to <i>Pseudomonas aeruginosa</i> PA14 infection in a dose-dependent manner. This protective effect was mediated not through direct antimicrobial activity, but rather <i>via</i> upregulation of the antimicrobial peptide <i>irg-1</i> and reduction of intestinal bacterial load. Mechanistically, resveratrol activated the ATFS-1-dependent UPR^mt pathway, leading to increased expression of ATFS-1 and its downstream immune- and mitochondrial-protective genes. In human A549 cells, resveratrol attenuated <i>P. aeruginosa</i> PA14 cytotoxicity by activating the UPR^mt through ATF5. The conservation of this mechanism was further validated in mice, where resveratrol treatment improved survival, reduced bacterial burden in lung tissue, and upregulated mitochondrial-protective genes. Our study identifies the ATFS-1/ATF5-UPR^mt axis as a novel mechanism through which resveratrol enhances innate immunity, providing a foundation for developing natural compound-based anti-infective therapies. These findings advance our understanding of plant polyphenols in immune regulation and offer potential strategies to address antibiotic resistance.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of <i>ex vivo</i> VEGF-induced angiogenesis by tyrosol and hydroxytyrosol: a quantitative three-dimensional mouse aortic ring model.","authors":"Ana B Cerezo, Marta Gallardo-Fernandez, Álvaro Santana-Garrido, Ruth Hornedo-Ortega, Carmen M Vázquez, Ana M Troncoso, M Carmen Garcia-Parrilla","doi":"10.1039/d5fo02820e","DOIUrl":"https://doi.org/10.1039/d5fo02820e","url":null,"abstract":"<p><p>Mediterranean diet foods such as olives, virgin olive oil and wine are sources of tyrosol (TOL) and hydroxytyrosol (HT), which are bioactive compounds. HT has already shown <i>in vitro</i> anti-angiogenic effects in HUVECs. Since TOL is structurally closely related to HT, the aim of the present study was firstly to evaluate the anti-angiogenic properties of TOL regarding inhibition of VEGF-induced VEGFR2 phosphorylation as well as its effects on the intracellular signaling cascade (PLCγ1, Akt and eNOS). Additionally, this paper aims to demonstrate the anti-angiogenic effects of HT and TOL using the <i>ex vivo</i> gold standard mouse aortic ring model. Our results demonstrated that TOL significantly inhibits VEGF-induced VEGFR2 activation in HUVECs, with an IC₅₀ value of 38.33 μM. Additionally, TOL completely blocked PLCγ1 activation, a key component of the VEGFR-2-mediated signalling pathway, while simultaneously increasing the phosphorylation of Akt and eNOS, critical molecules in the regulation of angiogenesis and vasodilation. This study is the first to use the mouse aortic ring model to demonstrate the anti-angiogenic effects of TOL and HT. A significant reduction of capillary sprouting at 68% and 96% was observed for TOL and HT, respectively. These results not only support the potential of TOL and HT as natural anti-angiogenic agents but also offer a new perspective on how diet, especially the Mediterranean diet, may influence the prevention and treatment of angiogenesis-related diseases, such as cancer and cardiovascular diseases.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric and ratiometric fluorescent probes for sensitive visualization of hydrazine in water and in living cells.","authors":"Weijie Chang, Wenyi Yang, Xiaoxiao Wang, Fanglong Xu, Weibing Zhang, Junhong Qian","doi":"10.1039/d5ay01337b","DOIUrl":"10.1039/d5ay01337b","url":null,"abstract":"<p><p>Taking account of the strong nucleophilicity of hydrazine, two fluorescent probes, DEA-A and Jul-B, were designed and synthesized. These probes employed 2,3,5,6-1<i>H</i>,4<i>H</i>-tetrahydroquinolo[9,9a,1-gh]coumarin (Jul-B) or <i>N</i>,<i>N</i>-diethyl-7-aminocoumarin (DEA-A) as the fluorophore, and 2-benzothiazole acetonitrile as the recognition moiety for N₂H₄. The formation of hydrazones weakened the intramolecular charge transfer (ICT) process in the probes, leading to blue-shifts of more than 100 nm in both absorption and emission. DEA-A and Jul-B demonstrated notable colorimetric and ratiometric fluorescence responses to N₂H₄, which enabled us to realize the identification of hydrazine using the \"naked-eye\". In the case of DEA-A, the <i>I</i>₅₁₃/<i>I</i>₆₁₆ ratio exhibits a linear relationship with N₂H₄ concentration in the range of 1.0-150 μM, and the detection limit for N₂H₄ was determined to be 0.10 μM. With the assistance of smartphones, N₂H₄ concentrations in realistic samples were visualized <i>via</i> RGB image analysis. In addition, both probes were applied in ratiometric fluorescence imaging of N₂H₄ in the living L929 cell line.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8120-8126"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel rapid visual LAMP platform for detection of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) in food.","authors":"Kaiwei Hu, Qiuyan Chen, Xiaozhen Xu, Qijie Lin, Jianmin Zhang, Xiaowei Wu, Chenggang Xu","doi":"10.1039/d5ay01288k","DOIUrl":"10.1039/d5ay01288k","url":null,"abstract":"<p><p>Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) has been increasingly identified as a significant foodborne pathogen that can causes severe infections in humans. Furthermore, MRSA has become a pervasive presence in both food products and healthcare environments. In order to address the lack of rapid, on-site detection methods capable of producing visual readouts for MRSA, a novel detection system targeting the <i>nuc</i> (<i>S. aureus</i>-specific) and <i>mecA</i> (methicillin-resistance) genes was developed using an enzyme-primed probe-based loop-mediated isothermal amplification (EP-LAMP) method. This method builds upon conventional loop-mediated isothermal amplification (LAMP) by incorporating a circular probe and the RNase H2 enzyme. The process of hybridization of the ribonucleotide within the probe to the complementary deoxyribonucleotide sequence of the target gene is followed by RNase H2 cleavage of the ribonucleotide site. This results in the separation of the fluorescent reporter from its quencher and subsequent signal generation. The results can be visually interpreted using a smartphone interface. For the <i>nuc</i> gene, the limit of detection (LOD) of the EP-LAMP method was 5.94 × 10¹ copies per μL for plasmid DNA and 4.17 × 10¹ CFU mL^-1 for genomic DNA. Similarly, the LOD for the <i>mecA</i> gene was 6.53 × 10¹ copies per μL for plasmid DNA and 4.17 × 10¹ CFU mL^-1 for genomic DNA. All reactions were completed within 30 minutes. In clinical sample testing, EP-LAMP exhibited 100% concordance with quantitative PCR (qPCR) results. The method's efficacy as a tool for MRSA detection in clinical settings is attributable to its high sensitivity, excellent specificity, and rapid turnaround time. Moreover, the visual readout facilitates straightforward result interpretation, enabling point-of-care diagnostics in resource-limited environments.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8224-8234"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-chip integration of electromembrane extraction and thin-film solid-phase microextraction using polyaniline/graphene oxide composite for determination of synthetic dyes in beverage and environmental samples.","authors":"Simin Darabi, Razieh Zamani, Yadollah Yamini","doi":"10.1039/d5ay01427a","DOIUrl":"10.1039/d5ay01427a","url":null,"abstract":"<p><p>In this study, an integration of on-chip electromembrane extraction (EME) and thin-film solid-phase microextraction (TF-SPME) was developed for the determination of cationic synthetic dyes, including methylene blue, malachite green, and rhodamine B in beverage and environmental samples. The device consisted of two separate chambers: one for the sample solution (donor phase) and one for acceptor phase. A conductive thin-film of polyaniline/graphene oxide (PANI/GO) nanocomposites was deposited electrochemically on a fluorine-doped tin oxide surface, which served as the TF-SPME sorbent and electrode in the acceptor chamber. During extraction, target analytes migrated under an electric field and were simultaneously adsorbed onto the PANI/GO coating. A desorption solvent was subsequently introduced into the acceptor phase to elute the analytes, which was analyzed by high-performance liquid chromatography with ultraviolet detection (HPLC-UV). Optimization of the key parameters affecting the extraction was carried out to maximize the efficiency. Under optimal conditions, limits of detection in the range of 1.5-3 μg L^-1 were achieved. The method exhibited linearity within 10-1000 μg L^-1 for malachite green and 5-1000 μg L^-1 for methylene blue and rhodamine B (<i>R</i>² ≥ 0.9963). The applicability of the proposed technique was evaluated by determining the model analytes in grape juice, fish farming river water, and textile industry wastewater, yielding relative recoveries ranging from 96.5% to 116.8%. Consequently, the integration of EME/TF-SPME and the use of PANI/GO in the acceptor phase were found to be successful in determination of cationic dyes in environmental samples.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8245-8255"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of vitamin E from dietary, circulation system, and supplementation on chronic obstructive pulmonary disease (COPD): a systematic review and meta-analysis.","authors":"Peixuan Tian, Hui Xia, Bihuan Hu, Yu Yang, Da Pan, Wang Liao, Shaokang Wang, Guiju Sun, Jing Sui","doi":"10.1039/d5fo03814f","DOIUrl":"https://doi.org/10.1039/d5fo03814f","url":null,"abstract":"<p><p><i>Background</i>: In recent years, the efficacy of antioxidants in the management of various diseases has garnered significant attention. Vitamin E, a natural supplement with potent antioxidant properties, has been utilized in the prevention of chronic obstructive pulmonary disease (COPD) and the treatment of patients with COPD. This systematic review and meta-analysis aimed to clarify the association between vitamin E consumption and COPD. <i>Methods</i>: We conducted a systematic search of eligible studies from PubMed, Web of Science, Scopus, and the Cochrane Library databases, performed a meta-analysis using random-effects models and assessed the association of vitamin E dietary intake, serum levels, and supplementation with COPD. <i>Results</i>: The study included a total of 10 studies involving 65 425 participants. The meta-analysis results indicated that the odds ratio (OR) between vitamin E intake and the onset risk of COPD was 0.60 (95% CI: 0.46-0.80, <i>P</i> < 0.0001), which indicated a significant protective effect. However, no significant correlation was observed between vitamin E consumption and the risk of death from COPD (OR = 0.79, 95% CI = 0.55-1.12, <i>P</i> = 0.07). In addition, subgroup analysis revealed that different subtypes of vitamin E, such as vitamin E (OR = 0.78, 95% CI = 0.68-0.91, <i>P</i> = 0.01) and α-tocopherol (OR = 0.43, 95% CI = 0.23-0.84, <i>P</i> = 0.03), as well as various sources of vitamin E, including dietary intake (OR = 0.68, 95% CI = 0.54-0.86, <i>P</i> = 0.007), serum levels (OR = 0.49, 95% CI = 0.25-0.98, <i>P</i> = 0.02), and supplementation (OR = 0.90, 95% CI = 0.82-0.99, <i>P</i> = 0.03) were all significantly inversely associated with COPD. <i>Conclusion</i>: Vitamin E consumption, including the intake of different subtypes and from various supplementation sources, demonstrates a protective effect against the risk of COPD and may contribute to improved clinical outcomes in patients with COPD. However, further research is required to fully elucidate the potential role and mechanism of vitamin E in COPD.</p>","PeriodicalId":77,"journal":{"name":"Food & Function","volume":" ","pages":""},"PeriodicalIF":5.4,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The visual detection of amoxicillin using a dual-mode probe based on CQD-doped MnO₂ nanospheres.","authors":"Zhenhua Wu, Nani Fu, Xianqin Han, Ting Yan, Jiaying Zheng, Jintao Yi","doi":"10.1039/d5ay01256b","DOIUrl":"10.1039/d5ay01256b","url":null,"abstract":"<p><p>Amoxicillin (AMO), a broad-spectrum penicillin antibiotic, has been widely employed for the treatment of bacterial infections. However, the unreasonable use of amoxicillin may cause a serious threat to human health, and its visual determination remains a challenge. Herein, a MnO₂@CQD probe was used for the dual-mode detection of AMO, which was synthesized by doping CQDs into MnO₂ nanospheres. In the colorimetric mode, MnO₂@CQDs oxidized colorless 3, 3', 5, 5'-tetramethylbenzidine (TMB) into yellow oxidized TMB (oxTMB). In the presence of AMO, AMO could deoxidize oxTMB to cause multiple color changes from yellow to colorless. In the fluorescence mode, MnO₂@CQDs initially exhibited quenched fluorescence due to the presence of MnO₂. Upon the addition of AMO, MnO₂ was reduced to Mn²⁺ ions, and then CQDs were released and their fluorescence was recovered at an emission wavelength of 450 nm. The colorimetric detection range was wide from 0.005 to 0.09 μM, and the detection limit was 1.6 nM. With increasing concentration of AMO, the color changed from yellow to colourless, which allowed naked eye observation. The fluorescence method was rapid, requiring only 1 min, and exhibited a linear detection range from 0.003 to 0.045 μM, with a detection limit as low as 1 nM. In addition, a paper-based sensing platform was constructed, where fluorescence intensity transitioned from dark to blue with increasing AMO levels. The RGB analysis demonstrated that AMO concentration could be discriminated effectively using a smartphone-assisted detection system. The MnO₂@CQD sensor showed excellent selectivity and specificity for AMO colorimetric and fluorescence detection. Moreover, the probe exhibited good practicability in tap water, milk and serum, with results comparable to those obtained by high-performance liquid chromatography. Overall, the MnO₂@CQD dual-mode probe provides an efficient, convenient and rapid strategy for the visual and quantitative detection of AMO in complex environments.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8171-8179"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145204953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a new electrochemical sensor for serotonin detection based on a manganese(III) porphyrin complex.","authors":"Sana Askri, Maroua Moslah, Hayfa Mkacher, Habib Nasri, Chérif Dridi","doi":"10.1039/d5ay01128k","DOIUrl":"10.1039/d5ay01128k","url":null,"abstract":"<p><p>We report the development of a novel electrochemical sensor for serotonin (5-HT) detection, based on a glassy carbon electrode (GCE) modified with a newly synthesized manganese(III) porphyrin complex (triflato)[(<i>meso</i>-tetra(<i>para</i>-tolylphenyl)porphyrinato)]manganese(III), denoted as [Mn^III(TTP)(SO₃CF₃)]. To the best of our knowledge, this is the first study utilizing this specific complex as an electroactive material for serotonin sensing. The complex was thoroughly characterized by FTIR, Raman, and UV-visible spectroscopy. Key experimental parameters, including the mass percentage of the modifier, pH, and scan rate, were systematically optimized. The electrochemical detection of 5-HT was performed using differential pulse voltammetry (DPV), yielding a limit of detection of 5.3 μM and a linear detection range of 10-250 μM. The modified electrode showed high selectivity toward serotonin in the presence of common interferents such as dopamine, glucose, uric acid, ascorbic acid, NaCl, and KCl. It also demonstrated excellent reproducibility (RSD = 1.34%) and repeatability (RSD = 0.8%). Moreover, the sensor's practical applicability was validated through successful detection of 5-HT in artificial urine samples. The originality of the sensing interface and the promising analytical performance highlight the potential of this platform for clinical diagnostics and neurochemical research.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8212-8223"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}