The Royal Society of Chemistry最新文献

{"title":"A cyclization-driven ratiometric fluorescent probe for hypochlorite detection: targeted sensing and visualization in plant samples and living cells.","authors":"Vinayagam Dhandapani, Subramanian Karpagam","doi":"10.1039/d5ay01123j","DOIUrl":"10.1039/d5ay01123j","url":null,"abstract":"<p><p>Most ClO^- sensing probes are limited by having only a single active site, which often results in structural breakdown after ClO^- interaction. To overcome this challenge, a novel ratiometric fluorescent probe, PBT-2, featuring a phenothiazine-modified thiophene-benzothiazole hydrazone scaffold, was synthesized and characterized for efficient ClO^- sensing. The probe operates <i>via</i> an internal charge transfer (ICT) \"turn-off\" mechanism, exhibiting rapid fluorescence quenching from light yellow to colorless within 15 seconds under optimized conditions. It offers high selectivity and an ultra-low detection limit of 2.2 nM. Mechanistic insights revealed that the sensing involves ClO^--triggered oxidation followed by intramolecular cyclization, supported by ¹H-NMR titration, DFT calculations, and ESI-MS analysis. The probe demonstrated its utility in visualizing ClO^- in plant roots, aiding in the study of hypochlorite's physiological roles, and was further validated in living cells, where it enabled real-time fluorescence imaging of intracellular ClO^- with excellent biocompatibility. In addition, PBT-2 was successfully applied to detect ClO^- in commercial bleach and environmental water samples, showcasing outstanding photophysical and sensing performance across diverse matrices.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8256-8265"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145248911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on egg yolk color detection based on near infrared spectroscopy and machine vision.","authors":"Yukuan Wen, Guimei Dong, Weijian Yin, Renjie Yang, Liu'an Li, Xiaoxue Yu, Yuan Li, Yaping Yu","doi":"10.1039/d5ay01039j","DOIUrl":"10.1039/d5ay01039j","url":null,"abstract":"<p><p>Yolk color is a key indicator of egg quality, as customers prefer eggs with intensely yellow yolks, which also signal nutrient richness. At present, the commonly used method for yolk color detection is to open the eggs and evaluate the yolk color using the Roche yolk color fan (RYCF), so developing a non-destructive method for discrimination of yolk color is of great significance. In order to overcome the human subjectivity associated with RYCF based on yolk color scoring, a machine vision method was built to classify the yolk color grades more objectively and precisely. In this work, a total of 150 egg samples with yolk color scores from 5 to 11 were collected and the near-infrared (NIR) spectral data of intact eggs and egg yolks were gathered independently, while the true scores of yolk color grades were acquired using the machine vision system as the target set for modeling. Finally, different regression prediction models for egg yolk color grades were constructed using chemometric Partial Least Squares (PLS) and machine learning techniques, such as Temporal Convolutional Network - Gated Recurrent Unit-Attention (TCN-GRU-Attention), Least Squares Support Vector Machines (LSSVM) and Convolutional Neural Network-Bidirectional Long Short Term Memory-Adaptive Boosting (CNN-BiLSTM-Adaboost). For the intact egg and separated yolk spectral data, the results show that the PLS model achieved the best prediction accuracy in the test set, with <i>R</i>² values of 0.9035 and 0.9274, and the root mean square errors (RMSE) were 0.3665 and 0.2933, respectively, which accomplished the non-destructive quantitative detection of egg yolk color scores.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8190-8201"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in metal-catalyzed strategies for carbohydrate functionalization.","authors":"Ahmed El Rhaz, Lisa Bonin, Ameni Hadj Mohamed, Samir Messaoudi","doi":"10.1039/d5cc04316f","DOIUrl":"10.1039/d5cc04316f","url":null,"abstract":"<p><p>Transition metal catalysis provides a versatile and powerful platform for the construction of structurally complex molecules. In recent years, its application has been successfully extended to the functionalization of carbohydrates, significantly expanding the toolbox of catalytic strategies available for sustainable synthesis. This review offers a comprehensive and up-to-date overview of recent advances in metal-catalyzed carbohydrate transformations, emphasizing key methodological innovations and their relevance to synthetic applications.</p>","PeriodicalId":67,"journal":{"name":"Chemical Communications","volume":" ","pages":"16331-16353"},"PeriodicalIF":4.2,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a novel LC-MS/MS method for simultaneous quantification of alvocidib and its glucuronide: application to pharmacokinetic and tissue distribution study in rats.","authors":"Eric Asare, Ting Du, Yuan Chen, Shalom Emmanuel, Huan Xie, Dong Liang, Song Gao","doi":"10.1039/d5ay01143d","DOIUrl":"10.1039/d5ay01143d","url":null,"abstract":"<p><p>Alvocidib, an approved drug for the treatment of acute myeloid leukemia, undergoes glucuronidation metabolism. The purpose of this study is to develop a robust and sensitive LC-MS/MS method to simultaneously quantify alvocidib and its metabolite, alvocidib-glucuronide (Alv-G), and apply this method in a pharmacokinetic and tissue distribution study in rats. A UHPLC system coupled to an AB Sciex QTrap 4000 triple quadrupole mass spectrometer was used for the analysis. The analytes were separated using a Biphenyl column with 0.1% formic acid in water and acetonitrile as the mobile phases and were quantified under positive electrospray ionization (ESI) mode using a multiple reaction monitoring (MRM) approach. Protein precipitation with acetonitrile was used in sample processing. The method showed a good linearity within the range of 2.4-5000.00 nM for both analytes. The method was reproducible, with intra and inter-day accuracy and precision ranging from 84-98% and <13% respectively. The extraction recovery and matrix effect achieved acceptable ranges (82-105%). Stability studies demonstrated that they were stable under bench-top (91-99%), freeze-thaw (83-110%), and long-term storage conditions (80-106%). The PK results showed that after oral administration of alvocidib, both alvocidib and Alv-G were detected in plasma with the parent drug showing highest distribution in the colon, and lowest in the small intestine. In contrast, Alv-G showed the highest distribution in the small intestine and the lowest amount in the colon compared to the other tissues. This method is applicable for PK and tissue distribution studies to quantify both alvocidib and its glucuronide.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8142-8149"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and accurate detection of crop viruses by nano-electrochemical sensors.","authors":"Anyou Xie, Weihong Wu","doi":"10.1039/d5ay01205h","DOIUrl":"10.1039/d5ay01205h","url":null,"abstract":"<p><p>This review aims to critically examine the development, capabilities, and future prospects of nano-electrochemical sensors as a next-generation solution for the rapid and accurate detection of crop viruses. The motivation stems from the urgent need to overcome the shortcomings of conventional diagnostic tools-such as ELISA and PCR-which, while accurate, suffer from drawbacks in speed, portability, and adaptability to evolving viral threats. We first introduce the fundamental architecture and working principles of electrochemical biosensors, emphasizing key transduction mechanisms including amperometry, voltammetry, and electrochemical impedance spectroscopy. A major focus is placed on the role of nanomaterials-such as gold nanoparticles, carbon-based nanostructures, quantum dots, metal-organic frameworks (MOFs), and MXenes-in enhancing sensor performance through improved surface area, electron transfer, and bioreceptor immobilization. Real-world applications are highlighted through recent advances in detecting agriculturally important viruses like tobacco mosaic virus, plum pox virus, and Citrus tristeza virus, with emphasis on sensitivity, selectivity, and response time. The review also explores current limitations, such as sensor reproducibility, field stability, and biofouling, as well as emerging directions including multiplexed detection systems and integration with Internet of Things (IoT) networks and artificial intelligence (AI) platforms for real-time crop health monitoring. By synthesizing technological advances and outlining actionable research pathways, this review underscores the transformative potential of nano-electrochemical sensors in plant virology and precision agriculture.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8083-8102"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nickel-catalyzed [4+2] cycloadditions of β-ketoesters and alkynes.","authors":"Tongtong Ni, Xuefeng Xu, Wenguang Li, Shiyuan Li, Heyun Sheng, Xu Zhang","doi":"10.1039/d5cc04128g","DOIUrl":"https://doi.org/10.1039/d5cc04128g","url":null,"abstract":"<p><p>The nickel-catalyzed synthesis of pyranone derivatives from β-ketoesters and alkynes has been reported. This reaction showcases remarkable versatility as it can incorporate a wide range of β-ketoesters and demonstrates the capacity to tolerate numerous functional groups and complex molecular structures.</p>","PeriodicalId":67,"journal":{"name":"Chemical Communications","volume":" ","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of naphth[2,1-<i>a</i>]azulenes <i>via</i> [8 + 2] cycloaddition of 2<i>H</i>-cyclohepta[<i>b</i>]furan-2-ones, and their reactivity, optical, and structural properties.","authors":"Taku Shoji, Akari Yamazaki, Miku Yoshida, Masafumi Yasunami Deceased, Shigeki Mori, Tetsuo Okujima, Takumi Kamayachi, Ikumi Uchiyama, Ryuta Sekiguchi, Shunji Ito","doi":"10.1039/d5ob01492a","DOIUrl":"https://doi.org/10.1039/d5ob01492a","url":null,"abstract":"<p><p>A concise and efficient synthetic route to naphth[2,1-<i>a</i>]azulenes was developed <i>via</i> [8 + 2] cycloaddition of 2<i>H</i>-cyclohepta[<i>b</i>]furan-2-ones with enamines or a silyl enol ether derived from α-tetralones. A diverse array of functionalized naphth[2,1-<i>a</i>]azulenes was further obtained through functionalization reactions, including electrophilic substitution, homocoupling, and cross-coupling reactions. Comprehensive spectroscopic analyses, including ¹H NMR, UV-Vis, fluorescence spectroscopy, and single-crystal X-ray diffraction, along with NICS calculations, revealed reduced aromaticity of the azulene core and a strong dependence of optical properties on the substitution pattern. Furthermore, naphth[2,1-<i>a</i>]azulenes exhibited pronounced halochromic behaviour and fluorescence in acidic media. Electrochemical studies indicated predominantly irreversible redox behaviour, however, certain donor-acceptor systems displayed quasi-reversible responses attributed to slow electron transfer kinetics.</p>","PeriodicalId":96,"journal":{"name":"Organic & Biomolecular Chemistry","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elemental perturbation in <i>Arabidopsis thaliana</i> under low temperature stress using ICP-MS based subcellular metallomics.","authors":"Xinfeng Li, Xuehong Wei, Yaoming Liu, Danyu Xia, Lijuan Li, Huilan Yi, Junjie Zhang","doi":"10.1039/d5ay01225b","DOIUrl":"10.1039/d5ay01225b","url":null,"abstract":"<p><p>The mechanism of how plants respond to low temperature stress is very important for resistance improvement and yield increase. As far as we know, no research has reported a comprehensive analysis of elemental perturbation in plants under low temperature stress by metallomics at the subcellular level until now. In this study, a subcellular metallomics analytical method for <i>Arabidopsis thaliana</i> based on differential centrifugation-microwave digestion coupled with inductively coupled plasma mass spectrometry was developed. The correlation coefficients <i>R</i>² of 69 elements were all above 0.9928 in the corresponding linear ranges of 3 orders of magnitude. The limits of detection (LODs) ranged from 0.000005 to 1.535 μg g^-1, and limits of quantification (LOQs) ranged from 0.00002 to 5.118 μg g^-1. Forty elements in 5 subcellular fractions could be reasonably determined with a repeatability that the relative standard deviations (RSDs) of elemental contents below 30% reached 66.8%. The method accuracy was validated by a certified reference material (CRM) (GBW10028). Under low temperature stress, the absolute contents of 26 elements in 51 subcellular fractions changed significantly with fold changes ranging from 0.18-3.16 between the low temperature group and the control group. Three main trends of significant elemental perturbation in subcellular components under cold stress were found: the contents of some elements (Co, Fe, Rb and W) increased in chloroplasts; the contents of elements Eu, Nb, Th, Ti, Dy, Lu, Y, Cs, Si and Yb increased in cell wall components; the contents of elements Ba, K, Mg, Mn, P, Ca, Sr, Mo and Zn decreased in the nuclear component but increased in the chloroplast and mitochondrial components. The method was proven to be sufficiently accurate, stable and reliable with wide elemental coverage, high throughput and high sensitivity, and it could serve as a good technical reference for obtaining multi-elemental characteristics at the subcellular level in plants.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8202-8211"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-assembly of microscale architectures with nanoscale inclusions.","authors":"Alicia Naranjo, Marine Batista, Emilio M Pérez, Mark W Rutland","doi":"10.1039/d5nh00356c","DOIUrl":"https://doi.org/10.1039/d5nh00356c","url":null,"abstract":"<p><p>We hypothesise that the recent discovery of nanodomains at the air-water interface can be leveraged to nano-functionalize surfaces through casting with incorporated functional species. The interfacial self-assembly of the amphiphilic molecules, 18-methyleicosanoic acid 18-MEA and 4-(tetradecyl)benzene diazonium tetrafluoroborate TDDS, at the air-water interface and cast on silicon wafer has been investigated using Langmuir-Blodgett (LB) techniques and atomic force microscopy. The impact of composition and surface pressure (SP) on the formation of nanodomains and microstructures was examined. TDDS (which can be used to modify the electronic structure of graphene) behaves as a co-surfactant in the 18-MEA film at low concentrations, facilitating the formation of homogeneous nanodomains with functional capacity. At higher TDDS concentrations, there is evidence for phase separation in the domains, and the TDDS furthermore partitions to the aqueous phase at higher pressures. By manipulating the 18-MEA:TDDS ratio and SP, regular nano-patterns can be transitioned into novel 2D structures reminiscent of 3D water-in-oil-in-water (W/O/W) analogues (\"cookie systems\"), offering a versatile strategy for designing nanoarchitectures with potential applications in graphene patterning.</p>","PeriodicalId":93,"journal":{"name":"Nanoscale Horizons","volume":" ","pages":""},"PeriodicalIF":6.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is it possible to distinguish virgin <i>versus</i> pyrolytic recycled styrene by determining the intramolecular ¹³C distribution?","authors":"Maxime Julien, Carlos Andrés Rincón Mejía, Mathilde Grand, Anne-Marie Schiphorst, Estelle Martineau, Serge Akoka, Thibaud Herbst, Gérald S Remaud","doi":"10.1039/d5ay01257k","DOIUrl":"https://doi.org/10.1039/d5ay01257k","url":null,"abstract":"<p><p>The accumulation of plastic waste presents a significant global environmental challenge, as these materials are highly resistant to degradation. Polymer recycling offers a promising solution by repurposing plastic waste and reducing reliance on fossil hydrocarbons. Most manufacturing companies that use plastics as raw materials are aware of the societal and environmental issues. With the support of governments, they all are striving to increase the use of recycled materials in the short term, the higher cost of recycled polymers compared to virgin materials (directly derived from petroleum) has led to concerns about counterfeiting. Currently, plastic traceability relies on manufacturers' documentation and declarations, highlighting the urgent need for authentication tools to verify the origin of polymers and ensure the integration of recycled materials in industrial applications. Stable isotopes are widely used to authenticate the origins of various substances, such as pharmaceuticals, food and beverages, and they hold great potential for investigating the provenance of polymers. In this study, virgin styrene and pyrolysis-recycled samples were analysed using ¹³C position-specific isotope analysis performed through nuclear magnetic resonance spectrometry (isotopic ¹³C NMR). The detected ¹³C intramolecular distribution profiles revealed significant differences between the two sources, showing that polystyrene recycling induces an isotope effect. These preliminary findings are highly promising for authenticating recycled styrene. Furthermore, the substantial intramolecular ¹³C composition variations observed in the samples could potentially enable the quantification of recycled and virgin polymers in composite materials.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}