The Royal Society of Chemistry最新文献_第4页

Emerging investigator series: post-wildfire sediment geochemical characterization reveals manganese reactivity and a potential link to water quality impairment in the Gallinas Creek watershed, New Mexico. 新兴研究者系列：野火后沉积物地球化学特征揭示了新墨西哥州加利纳斯河流域锰的反应性和与水质损害的潜在联系。

IF 3.9 3区环境科学与生态学

Environmental Science: Processes & Impacts Pub Date : 2025-10-16 DOI: 10.1039/d5em00326a

Elizabeth J Tomaszewski, Sheila F Murphy, Johanna M Blake, Michelle I Hornberger, Gregory D Clark

{"title":"Emerging investigator series: post-wildfire sediment geochemical characterization reveals manganese reactivity and a potential link to water quality impairment in the Gallinas Creek watershed, New Mexico.","authors":"Elizabeth J Tomaszewski, Sheila F Murphy, Johanna M Blake, Michelle I Hornberger, Gregory D Clark","doi":"10.1039/d5em00326a","DOIUrl":"https://doi.org/10.1039/d5em00326a","url":null,"abstract":"Water quality post-wildfire is often impaired by increased turbidity and elevated concentrations of elements such as manganese (Mn) and iron (Fe). Precipitation events exacerbate these issues, due in part to increased erosion and transport of sediment from hillslopes to surface water. Both Mn and Fe are major redox-active elements in sediments that drive a variety of biogeochemical cycles, precipitate adsorptive phases, and can themselves be drinking water contaminants. By investigating Mn and Fe sediment geochemistry in post-wildfire sediment deposits, related water quality hazards can be assessed. To establish and strengthen this connection, we analyzed the geochemistry of sediment deposits and surface water in the Gallinas Creek watershed, New Mexico over 1.5 years post-wildfire. Analyses included particle size analysis, water extractions, sequential extractions and aqua regia extractions to determine metal partitioning in sediment deposits. Data demonstrate Mn concentrations were distributed across labile and reactive fractions, such as the exchangeable and oxyhydroxide fractions, while Fe concentrations were mainly associated with the residual fraction. Manganese concentrations in aqua regia extractions and several fractions of sequential extractions were also strongly and significantly correlated with fine-grained sediment while the same pools of Fe concentrations were not. Dissolved Mn concentrations in surface water were elevated (>50 μg L-1) multiple times over the 1.5 years post-wildfire, highlighting a relationship between sediment geochemistry and water quality. This work shows Mn in sediments mobilized post-wildfire has an influence on water quality and highlights how further investigation into Mn sediment redox processes and mineralogy post-wildfire can inform risk assessments and resource management.","PeriodicalId":74,"journal":{"name":"Environmental Science: Processes & Impacts","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Ultrathin DNA-copper nanosheets with antibacterial and anti-biofilm activity for treatment of infected wounds. 更正：超薄dna -铜纳米片具有抗菌和抗生物膜活性，用于治疗感染伤口。

IF 6.6 2区材料科学

Nanoscale Horizons Pub Date : 2025-10-16 DOI: 10.1039/d5nh90062j

Fangfang Chen, Mengyan Lei, Jing Luo, Jiaqi Li, Jinfang Wang, Nan Zhang, Xinyi Li, Nan Jia, Xiangyuan Ouyang, Huaiyu Bu

引用次数: 0

Sensitive detection of bisulfite by a novel quinoxalinyl-based fluorescent probe and its applications in food detection and bioimaging. 新型喹啉基荧光探针对亚硫酸盐的灵敏检测及其在食品检测和生物成像中的应用。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-10-16 DOI: 10.1039/d5ay01291k

Lewei Gao, Mengdi Zhao, Yue Gu, Shuai Gong, Yueyin Liang, Zhiyuan Meng, Yixin Yang, Zhonglong Wang, Shifa Wang

引用次数: 0

Considerations for deconvolution of oligonucleotide mass spectra in quality control. 质量控制中寡核苷酸质谱反褶积的考虑。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-10-16 DOI: 10.1039/d5ay01223f

Michael Rühl

{"title":"Considerations for deconvolution of oligonucleotide mass spectra in quality control.","authors":"Michael Rühl","doi":"10.1039/d5ay01223f","DOIUrl":"10.1039/d5ay01223f","url":null,"abstract":"Oligonucleotide therapeutics and mass spectrometry are evolving fields in both research and pharmaceutical development. A proper data evaluation needs to be ensured, especially in a regulated environment. Deconvolution is often applied to derive the intact mass from electrospray ionization (ESI) mass spectra. The type of deconvolution algorithm applied as well as the used parameters can affect the outcome of the analysis. This study aims to show differences in the deconvolution algorithm in terms of mass accuracy and how the input mass range affects the mass accuracy and the detected abasic impurities and adducts for polydeoxythymidine (poly-dT) samples with 20, 48, 100 and 200 nucleotides (nts). While Bayesian deconvolution is more suitable for analytes up to ∼15 000 Da in terms of mass accuracy, maximum entropy deconvolution fits best for larger oligonucleotides, with a mass accuracy of 1.6 ppm for a 100 (nts) poly-dT sample. The input mass range is the second important parameter to be tested, as low charge states tend to show more adducts and higher charge states tend to be prone for depurination and depyrimidation, leading to an overrepresentation of the impurity mass in the deconvoluted spectrum. This manuscript also highlights how the findings can be translated to a short DNA-based adjuvant and a sample single guide RNA.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8266-8271"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel rapid single-integrated immunoassay for plasma thrombomodulin: analytical performance and multi-center clinical validation. 一种新的血浆凝血调节素快速单一综合免疫分析方法：分析性能和多中心临床验证。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-10-16 DOI: 10.1039/d5ay01241d

Meng Liu, Shuaipeng Li, Chaolin Guo, Ziyan Xie, Qingxuan Miao, Yankun Yang, Jianqi Nie, Yang Sun, Zhonghu Bai

{"title":"A novel rapid single-integrated immunoassay for plasma thrombomodulin: analytical performance and multi-center clinical validation.","authors":"Meng Liu, Shuaipeng Li, Chaolin Guo, Ziyan Xie, Qingxuan Miao, Yankun Yang, Jianqi Nie, Yang Sun, Zhonghu Bai","doi":"10.1039/d5ay01241d","DOIUrl":"10.1039/d5ay01241d","url":null,"abstract":"Thrombotic diseases have emerged as a significant public health issue, with timely detection, diagnosis, and treatment being closely associated with patient prognosis. Consequently, the identification of highly sensitive and specific biochemical markers for thrombotic diseases holds substantial clinical importance. In this study, we developed a rapid, single-integrated quantitative detection method for thrombomodulin (TM). This method integrates all reagent components onto a single, sophisticated card strip, enabling the completion of the assay in merely 20 minutes. Such efficiency enhances the potential for early screening of thrombosis within community and primary care medical institutions. The analytical performance results indicate that the limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) were determined to be 0.5, 0.8, and 1.2 TU mL-1, respectively. The repeatability and precision of the method were found to be significantly below 10%, with a linear range extending from 1.2 to 200 TU mL-1. Accuracy evaluations revealed relative biases of -3.35% and 4.79% for the two reference materials tested. Furthermore, clinical trials conducted across two clinical research centers involved a total of 236 clinical plasma samples. The results of the clinical analysis demonstrated excellent consistency with the reference reagent (Sysmex). The receiver operating characteristic (ROC) curve and significance analysis indicated that this method possesses commendable specificity and sensitivity. In summary, the single-integrated immunoassay method developed in this study represents a rapid, convenient, accurate, and sensitive detection approach. It is particularly well-suited for the early screening and diagnosis of thrombotic diseases in community and primary healthcare institutions.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8150-8161"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of surface enhanced Raman spectroscopy (SERS) for the rapid detection and enumeration of probiotics in yogurt. 表面增强拉曼光谱（SERS）在酸奶中益生菌快速检测和计数中的应用。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-10-16 DOI: 10.1039/d5ay00931f

Kaitlyn Allen, Gene Ahlborn, Julia Wangsgard, Shintaro Pang

{"title":"Application of surface enhanced Raman spectroscopy (SERS) for the rapid detection and enumeration of probiotics in yogurt.","authors":"Kaitlyn Allen, Gene Ahlborn, Julia Wangsgard, Shintaro Pang","doi":"10.1039/d5ay00931f","DOIUrl":"10.1039/d5ay00931f","url":null,"abstract":"Probiotics are a critical component in fermented dairy products such as yogurt. An efficient method to enumerate probiotics in commercially produced yogurt is important, but traditional methods are time-consuming, sometimes taking up to 72 hours. The objective of this study was to develop a rapid method using surface-enhanced Raman spectroscopy (SERS) to identify and enumerate probiotic bacterial cells in yogurt. The identification and enumeration of three different probiotic bacterial strains, Lactobacillus acidophilus, Lactobacillus bulgaricus, and Streptococcus thermophilus were successfully conducted using a developed SERS protocol. L. bulgaricus and S. thermophilus were also identified from a yogurt matrix using a simple extraction procedure utilizing a Percoll gradient. Statistical spectral analysis including principal component analysis and partial least squares regression analysis allowed for the differentiation, characterization and quantification of probiotic strains. The limits of detection were 105 CFU mL-1 for L. acidophilus, 104 CFU mL-1 for L. bulgaricus, and 106 CFU mL-1 for S. thermophilus. Quantification capability was tested up to 108 cfu mL-1 and showed strong correlation coefficients (0.94, 0.98 and 0.95 respectively) between predicted and actual concentrations. The presence of probiotic mixtures were clearly detectable from a yogurt food matrix starting at 105 CFU mL-1. Sample analysis took less than three hours per sample set. Our results demonstrate the capability of SERS to detect and enumerate probiotics from a yogurt matrix rapidly.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8162-8170"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive quantitative detection technology for tetracycline and erythromycin based on allosteric transcription factors (aTFs) using SPR. 基于变构转录因子的四环素和红霉素的SPR灵敏定量检测技术。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-10-16 DOI: 10.1039/d5ay01317h

Yingzi Wang, Chen Zhao, Shang Wang, Bin Xue, Chenyu Li, Xi Zhang, Xiaobo Yang, Zhigang Qiu, Zhiqiang Shen, Jingfeng Wang

{"title":"A sensitive quantitative detection technology for tetracycline and erythromycin based on allosteric transcription factors (aTFs) using SPR.","authors":"Yingzi Wang, Chen Zhao, Shang Wang, Bin Xue, Chenyu Li, Xi Zhang, Xiaobo Yang, Zhigang Qiu, Zhiqiang Shen, Jingfeng Wang","doi":"10.1039/d5ay01317h","DOIUrl":"10.1039/d5ay01317h","url":null,"abstract":"Antibiotics are extensively utilized in agriculture and livestock farming; however, their environmental residues may foster the proliferation of drug-resistant bacteria and disrupt ecosystems, underscoring the necessity for sensitive detection technologies. Allosteric transcription factors (aTFs) represent a class of proteins that can specifically recognize small molecules and regulate gene expression. Owing to their high specificity and sensitivity, aTFs have been widely adopted in the development of biosensors. This study utilized the properties of allosteric transcription factors to alter their DNA affinity upon ligand binding. By measuring the changes in affinity of TetR and MphR before and after DNA binding using SPR, the study quantitatively analyzed the concentrations of tetracycline and erythromycin. Through SPR technology, quantitative detection of these affinity changes was achieved. Under optimized conditions regarding sample incubation time and system injection time, the affinity values exhibited changes of two orders of magnitude. The detection limits for tetracycline and erythromycin using this technique were determined to be 0.8 nM and 1 nM, respectively. These results indicate that the developed SPR detection method demonstrates high sensitivity and accuracy for detecting tetracycline and erythromycin, making it a promising tool for various applications, including environmental monitoring and quality control.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8180-8189"},"PeriodicalIF":2.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sustainable and highly sensitive voltammetric method for the simultaneous determination of indigo carmine and carmoisine using a boron-doped diamond electrode. 用掺杂硼的金刚石电极建立了一种同时测定靛蓝胭脂红和卡莫辛的高灵敏度伏安法。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-10-16 DOI: 10.1039/d5ay01336d

Bruna Coldibeli, Elen Romão Sartori

引用次数: 0

Non-destructive colorimetric detection of milk freshness by starch-alginate sensor beads stabilized by nanoemulsions. 纳米乳液稳定的淀粉-海藻酸盐传感器珠无损比色法检测牛奶新鲜度。

IF 2.6 3区化学

Analytical Methods Pub Date : 2025-10-16 DOI: 10.1039/d5ay01249j

E Aswathy, Lisa Sreejith

引用次数: 0

misteINK: a protein nanocage-based ink with reversible, stimuli-responsive color shifts. missteink：一种以蛋白质纳米为基础的墨水，具有可逆的、刺激反应的颜色变化。

IF 5.7 3区医学

Biomaterials Science Pub Date : 2025-10-16 DOI: 10.1039/d5bm01052g

Maika Yamashita, Norifumi Kawakami, Ryoichi Arai, Akihito Ikeda, Toshio Moriya, Toshiya Senda, Kenji Miyamoto

{"title":"misteINK: a protein nanocage-based ink with reversible, stimuli-responsive color shifts.","authors":"Maika Yamashita, Norifumi Kawakami, Ryoichi Arai, Akihito Ikeda, Toshio Moriya, Toshiya Senda, Kenji Miyamoto","doi":"10.1039/d5bm01052g","DOIUrl":"https://doi.org/10.1039/d5bm01052g","url":null,"abstract":"Dyes exhibiting polarity-dependent color changes, known as solvatochromism, have great potential for creating sensors, smart materials, and responsive coatings. However, full-range color shifts require a technique to disperse dyes across a wide range of solvent polarities, which remains a persistent challenge. For example, hydrophobic dyes often aggregate in water, preventing effective color shifts. Although surfactants can assist in dye dispersion, they can also prevent solvent molecules from accessing the dye. To address this, we used a 60-mer protein nanocage, TIP60, with a densely pyrene-modified interior surface. The modification did not induce protein denaturation, as monitored by small-angle X-ray scattering, and greatly increased the aqueous solubility of a hydrophobic solvatochromic dye, Nile Red (NR), while preserving its fluorescence. The NR-loaded solution appeared blue, reflecting the polar environment surrounding NR. Cryogenic electron microscopy suggested that the pyrenes interacted with each other to form a binding site for NR. This interaction also contributed to thermostability of TIP60 (65 °C to 86 °C) and stability against sodium dodecyl sulfate, as observed by electrophoresis experiments. When brushed onto plain copy paper, the NR-loaded nanocage appeared bluish-purple and shifted reversibly to purplish red upon heating, returning on cooling-presumably via nanocage dissociation and reassembly. The color change was also sensitive to humidity. We term this material \"misteINK\", a protein-based ink with reversible temperature- and humidity-dependent color changes. These findings demonstrate that a single-step interior modification enables the rational design of protein materials for tuning dye photophysics, providing a powerful strategy for designing protein-based functional materials.","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0