{"title":"纳米乳液稳定的淀粉-海藻酸盐传感器珠无损比色法检测牛奶新鲜度。","authors":"E Aswathy, Lisa Sreejith","doi":"10.1039/d5ay01249j","DOIUrl":null,"url":null,"abstract":"<p><p>The study presents the development of Ca<sup>2+</sup>-crosslinked starch-alginate beads encapsulating ternatin-loaded nanoemulsions (SAB) for rapid detection of milk spoilage. The beads exhibited strong, stable colorimetric responses (Δ<i>E</i> > 50) across the pH range (5-7), shifting from light blue in fresh milk, dark blue in spoiling milk and blue-grey in spoiled milk. Encapsulation of the nanoemulsion within the bead effectively prevented the leaching of ternatin, while structural integrity was confirmed by FTIR and TGA analyses. Dry SAB showed superior stability in liquids and 87% cell viability, confirming its non-toxic nature. Unlike conventional techniques, SAB enabled fast, non-destructive on-site spoilage monitoring, highlighting its potential for real-time food safety applications.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":"8115-8119"},"PeriodicalIF":2.6000,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Non-destructive colorimetric detection of milk freshness by starch-alginate sensor beads stabilized by nanoemulsions.\",\"authors\":\"E Aswathy, Lisa Sreejith\",\"doi\":\"10.1039/d5ay01249j\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The study presents the development of Ca<sup>2+</sup>-crosslinked starch-alginate beads encapsulating ternatin-loaded nanoemulsions (SAB) for rapid detection of milk spoilage. The beads exhibited strong, stable colorimetric responses (Δ<i>E</i> > 50) across the pH range (5-7), shifting from light blue in fresh milk, dark blue in spoiling milk and blue-grey in spoiled milk. Encapsulation of the nanoemulsion within the bead effectively prevented the leaching of ternatin, while structural integrity was confirmed by FTIR and TGA analyses. Dry SAB showed superior stability in liquids and 87% cell viability, confirming its non-toxic nature. Unlike conventional techniques, SAB enabled fast, non-destructive on-site spoilage monitoring, highlighting its potential for real-time food safety applications.</p>\",\"PeriodicalId\":64,\"journal\":{\"name\":\"Analytical Methods\",\"volume\":\" \",\"pages\":\"8115-8119\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Methods\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1039/d5ay01249j\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d5ay01249j","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Non-destructive colorimetric detection of milk freshness by starch-alginate sensor beads stabilized by nanoemulsions.
The study presents the development of Ca2+-crosslinked starch-alginate beads encapsulating ternatin-loaded nanoemulsions (SAB) for rapid detection of milk spoilage. The beads exhibited strong, stable colorimetric responses (ΔE > 50) across the pH range (5-7), shifting from light blue in fresh milk, dark blue in spoiling milk and blue-grey in spoiled milk. Encapsulation of the nanoemulsion within the bead effectively prevented the leaching of ternatin, while structural integrity was confirmed by FTIR and TGA analyses. Dry SAB showed superior stability in liquids and 87% cell viability, confirming its non-toxic nature. Unlike conventional techniques, SAB enabled fast, non-destructive on-site spoilage monitoring, highlighting its potential for real-time food safety applications.