Veterinary Sciences最新文献

{"title":"Cytologic and Cytochemical Characterization of Blue-Green Neutrophilic Inclusions in a Dog with Suspected Transfusion-Associated Hemolysis.","authors":"Hyun-Jeong Hwang, Myung Hyun Choi, Hyo-Sung Kim, Sun Hee Do","doi":"10.3390/vetsci13090985","DOIUrl":"10.3390/vetsci13090985","url":null,"abstract":"<p><p>An 11-year-old castrated male Maltese dog presented with acute lethargy and non-regenerative anemia. Despite immunosuppressive therapy and an initial packed red blood cell (pRBC) transfusion, hematocrit levels progressively declined after transient improvement. A second transfusion was administered following compatible crossmatch testing. Within 24 h of the second transfusion, a transfusion-associated hemolytic episode was suspected due to a rapid hematocrit decline, pigmenturia, hyperbilirubinemia, and autoagglutination. Peripheral blood smear revealed neutrophils containing coarse blue-green cytoplasmic inclusions. Special staining and autofluorescence microscopy supported lipid-rich, acid-fast, and autofluorescent properties of the inclusions. Prussian blue staining showed iron-positive granular material in the hemolytic sample, but the intraneutrophilic inclusions did not show distinct Prussian blue reactivity, making hemosiderin-rich sideroleukocytes unlikely to fully explain the findings. Similar inclusions have recently been characterized in dogs, mainly in association with severe hepatocellular injury, pancreatitis, and poor short-term outcomes; however, their occurrence during acute clinical deterioration associated with suspected post-transfusion hemolysis has not been specifically documented. The dog died three days after inclusion detection. This case expands the clinical settings in which blue-green leukocyte cytoplasmic inclusions may be encountered in dogs.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical Detection, In Situ Distribution, and Comparison of Tuft Cells and Other Selected Components of Mucosal Immunity in Nasal Turbinates of Piglets Challenged with Rotavirus from Sows Fed Vitamin A-Deficient Diets with or Without Vitamin A Supplementation.","authors":"Kwonil Jung, Anastasia N Vlasova, Linda J Saif","doi":"10.3390/vetsci13090987","DOIUrl":"10.3390/vetsci13090987","url":null,"abstract":"<p><p>Tuft cells are a rare type of epithelial cell that are essential to regulate innate and adaptive immune responses. Mucosal immunity is also composed primarily of IgA produced by B cells that is secreted onto mucosal surfaces as secretory IgA via the polymeric immunoglobulin receptor (pIgR). The homing of B cells in lymphoid organs is mediated by certain adhesion receptors, such as α4β7 and chemokine receptor 9 (CCR9), that bind to the specific vascular ligands, mucosal vascular addressin cell adhesion molecule 1 (MAdCAM-1) and chemokine ligand 25 (CCL25), respectively. It is unclear whether tuft cells, MAdCAM-1, CCL25, and pIgR are present or expressed in the nasal cavities of pigs. Our study aimed to develop immunohistochemistry (IHC) or immunofluorescence (IF) staining for the detection of tuft cells, MAdCAM-1, CCL25, pIgR, and IgA- or IgG-positive cells in porcine nasal turbinates. The tissues were acquired from the piglets of two different treatment groups in our previous studies that investigated the impact of oral vitamin A supplementation (VA) on the maternal immunity of vitamin A-deficient (VAD) sows and the passive protection of their piglets against rotavirus A (RVA): (1) VAD + VA + RVA and (2) VAD + RVA piglets. Our IHC staining revealed that low numbers of advillin-positive, bottle-shaped cells that morphologically resemble tuft cells were present in the mucosal epithelium and the mucous duct or glands. There were also moderate to large amounts of MAdCAM-1 and CCL25 in the endothelial cells lining the vascular structures present in the lamina propria of nasal turbinates and moderate to large amounts of pIgR in the turbinate mucosal epithelium. Moderate to high numbers of IgA- or IgG-positive cells were present in the lamina propria. There were no differences in IF- or IHC-positive scores or the positive cell numbers of all parameters tested between VAD + VA + RVA and VAD + RVA piglets, suggesting little or no effect of VA on the parameters tested. Our study demonstrated the use of IHC/IF staining methods for the detection of tuft cells, MAdCAM-1, CCL25, pIgR, and IgA- or IgG-positive cells in porcine nasal turbinates, expanding our understanding of the in situ distribution and comparison in the nasal cavity of pigs born to the sows fed vitamin A-deficient diets with or without vitamin A supplementation.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13611996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical Evaluation of RUNX2, Osteocalcin, and Vimentin in Feline Primary Pulmonary Carcinomas.","authors":"Alexandra Morar, Corina Toma, Dragoș Hodor, Mara-Georgiana Haralambie, Iulia Pană, Alexia-Teodora Hoța, Diana Bochynska, Claudiu Gal, Cornel Cătoi","doi":"10.3390/vetsci13090980","DOIUrl":"10.3390/vetsci13090980","url":null,"abstract":"<p><p>Feline primary pulmonary carcinomas (PPCs) occasionally exhibit a distinctive metastatic tropism for the distal digits, clinically recognized as feline lung-digit syndrome or MODAL syndrome. The molecular mechanisms driving this unique behavior remain poorly understood. This retrospective study evaluated 18 feline PPCs to investigate the expression of bone-related markers (RUNX2, osteocalcin), vimentin, Ki-67, MCK, and TTF-1. Cases were classified into PPCs with (<i>n</i> = 5) and without (<i>n</i> = 13) documented digital metastases. Adenosquamous carcinoma was the predominant histological subtype (61.1%); all tumors expressed MCK and TTF-1. While osteocalcin and aberrant vimentin expression were detected across both groups, they did not differ significantly between groups. Conversely, RUNX2 nuclear immunoreactivity was significantly elevated in cases with digital metastasis (<i>p</i> = 0.006). Furthermore, the evaluation of matched primary and metastatic lesions demonstrated that digital metastases retained the histological and immunophenotypic profiles of their primary tumors. Overall, this first characterization demonstrates that higher RUNX2 immunoreactivity was observed in primary pulmonary carcinomas from cats with documented digital metastasis. However, without the evaluation of extra-digital metastases, it remains undetermined whether this reflects a specific tropism for the digits or a general metastatic competence.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13611842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gram-Negative Bacterial Overgrowth Predominated by: Potential Association with Intestinal Barrier Impairment and Inflammatory Diarrhea in Cats.","authors":"Jing Wang, Xinhui Zhang, Xinran Song, Yan He, Wuying Lang, Xinyu Zhang","doi":"10.3390/vetsci13090976","DOIUrl":"10.3390/vetsci13090976","url":null,"abstract":"<p><strong>Background: </strong>Feline diarrhea is one of the most common gastrointestinal disorders encountered in veterinary clinics. Dysbiosis of Gram-negative bacteria is widely presumed to be a key cause of this disease, yet adequate in vivo evidence demonstrating its roles in provoking inflammation and impairing intestinal barrier integrity is still lacking. In this study, we investigated the effects of bacterial flora in cats with diarrhea on gut health in mice.</p><p><strong>Results: </strong>The results show the following: (1) Microorganisms in feces of diarrheal cats can cause weight loss in antibiotic-treated mice; the histopathological section of the colon showed that neutrophil aggregation occurred in the colon of mice in the fecal bacteria transplantation group; this indicates that gavage using fecal microbes from diarrheic cats could impair intestinal barrier integrity in antibiotic-treated recipient mice. (2) In addition, microbes in cats with diarrhea can induce inflammation in the colon and aggregation of macrophages, which may be caused by some Gram-negative bacteria in feces of cats with diarrhea. (3) The composition of the intestinal microbial community was analyzed by 16S rRNA gene sequencing, and it was found that the relative abundance of <i>Escherichia</i>-<i>Shigella</i> in feces of cats with diarrhea was significantly higher than that of normal cats. (4) Quantitative analysis of fecal samples revealed that diarrheic cats exhibited a significantly higher load of fecal Gram-negative bacteria compared with healthy cats, verifying that excessive colonization of Gram-negative bacteria may be highly correlated with feline diarrhea.</p><p><strong>Conclusion: </strong>In summary, fecal microbes from diarrheic cats are capable of triggering intestinal inflammation and barrier damage in antibiotic-treated mice. Excessive proliferation of Gram-negative bacteria, mainly <i>Escherichia</i>-<i>Shigella</i> species, may be highly associated with feline diarrhea.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13611937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Self-Directed Multimodal Digital Training on Veterinary Students' Ability to Interpret Equine Heart Sounds.","authors":"Chiara Bozzola, Dania Cingottini, Francesca Bindi, Micaela Sgorbini, Tommaso Vezzosi, Enrica Zucca","doi":"10.3390/vetsci13090981","DOIUrl":"10.3390/vetsci13090981","url":null,"abstract":"<p><p>Cardiac auscultation is a fundamental clinical skill for veterinary graduates; however, acquiring this competence remains challenging because opportunities for repeated exposure to a wide range of cardiac abnormalities may often be limited during undergraduate training. This study evaluated whether supplementing written self-directed learning with digital equine phonocardiographic recordings improves veterinary students' ability to recognize and interpret equine cardiac auscultation findings. Forty-one fourth-year veterinary students from the Universities of Milan and Pisa were voluntarily enrolled and randomly assigned to receive either written learning materials alone (Group 1, <i>n</i> = 21) or the same materials supplemented with digital phonocardiographic recordings (Group 2, <i>n</i> = 20). Participants completed a baseline test (T0). After a four-week self-directed learning period, 30 students (Group 1 = 15; Group 2 = 15) completed the post-training test (T1) and were included in the final analysis. The statistical analysis provided no significant differences for the total score (<i>p</i> = 0.064) or Q1 score (<i>p</i> = 0.349), whereas a significant difference was observed for Q2 score (<i>p</i> = 0.039), showing greater improvement in Group 2. These findings suggest a potential educational benefit of supplementing written learning materials with digital phonocardiographic recordings, particularly for the ability to distinguish among different cardiac abnormalities.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of Feline Hemotropic <i>Mycoplasma</i> from Blood Samples of Sick Cats: A Comparative Study Between a Commercial Nucleic Acid Test Card and Conventional Polymerase Chain Reaction.","authors":"Burin Nimsuphan, Sakchai Ruenphet, Chanya Kengradomkij, Nutsuda Klinkaew, Ketsarin Kamyingkird","doi":"10.3390/vetsci13090978","DOIUrl":"10.3390/vetsci13090978","url":null,"abstract":"<p><p>Feline hemotropic mycoplasmosis presents a diagnostic challenge in clinical practice because of the variable pathogenicity of <i>Mycoplasma</i> species and the limited sensitivity of conventional blood smear examination, highlighting the need for rapid and accurate point-of-care molecular diagnosis. This study aimed to evaluate the diagnostic performance of the Pluslife^® Feline Hemotropic <i>Mycoplasma</i> (FHM) nucleic acid test card and to determine the detection frequency of the pathogen among clinically ill cats presented to a veterinary teaching hospital in Bangkok, Thailand. Whole blood samples were collected from 114 clinically ill cats presented to Kasetsart University Veterinary Teaching Hospital between September 2025 and May 2026. The nucleic acid test card assay was evaluated against conventional PCR targeting the 16S rRNA gene as the reference method. PCR identified '<i>Candidatus</i> Mycoplasma haemominutum' (<i>C</i>Mhm) as the predominant <i>Mycoplasma</i> species (45.61%), followed by <i>Mycoplasma haemofelis</i> (<i>Mhf</i>; 5.26%) and '<i>Candidatus</i> Mycoplasma turicensis' (<i>C</i>Mt; 0.88%). Compared with conventional PCR, the nucleic acid test card assay demonstrated a sensitivity of 93.22%, specificity of 96.36%, overall accuracy of 94.74%, and precision of 96.49%, with results available within 30 min. Sequence analysis of discordant samples confirmed <i>C</i>Mhm in four false-negative specimens, suggesting that low bacterial burdens or other assay-related factors may have contributed to the discordant results. Overall, the Pluslife^® FHM assay demonstrated high diagnostic performance and may provide a practical rapid molecular point-of-care tool to support timely diagnosis and clinical decision-making in cats with suspected hemotropic mycoplasmosis.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NS1 of Duck Tembusu Virus Interacts with CCT2: Reduced CCT2 Recovery in LC3C Co-Immunoprecipitation In Vitro and the Proviral Role of CCT2 in DTMUV Infection.","authors":"Zekun Yang, Haixia Feng, Qiongjie Wang, Chengwei Xiang, Yin Han, Fei Xu, Mengyao Jing, Hongmei Li, Jianhua Liang, Xianming He, Ruiai Chen, Ting Xiong","doi":"10.3390/vetsci13090979","DOIUrl":"10.3390/vetsci13090979","url":null,"abstract":"<p><p>Regulation of Duck Tembusu virus (DTMUV) non-structural protein 1 (NS1) remains unclear. Exogenous and endogenous co-immunoprecipitation (Co-IP) assays demonstrated an association between the T-complex protein 1 subunit 2 (CCT2) and NS1. Further experiments mapped the NS1 region required for assembly of this complex to amino acid residues 172-352 of NS1.DTMUV increased CCT2 expression; CCT2 overexpression increased NS1 accumulation, whereas CCT2 knockdown delayed NS1 and envelope (E) protein accumulation. Although NS1 also associated with chaperonin-containing T-complex protein 1 subunit 6 (CCT6), CCT6 knockdown did not significantly alter NS1 messenger RNA (mRNA) or clearly change NS1 protein abundance. NS1 underwent lysine 48 (K48)- and lysine 63 (K63)-linked polyubiquitination. Increasing tripartite motif-containing protein 21 (TRIM21) reduced NS1 abundance and enhanced NS1-associated ubiquitin signals; the proteasome inhibitor MG132 promoted NS1 accumulation, which was highest with CCT2 co-expression, supporting proteasome-related turnover and CCT2-associated stabilization without establishing direct TRIM21 ubiquitination. CCT2 interacted with microtubule-associated protein 1 light chain 3C (LC3C), and DTMUV infection was associated with reduced late-stage lipidated LC3 (LC3-II) abundance and SQSTM1 mRNA expression. In dose-gradient Co-IP experiments, increasing NS1 expression was associated with a dose-associated reduction in CCT2 recovery in LC3C immunoprecipitates; the concomitant reduction in immunoprecipitated LC3C across the NS1 dose gradient precludes conclusions regarding direct competition or a binding capacity-dependent mechanism. These findings indicate that CCT2 is a DTMUV-associated host factor capable of stabilizing intracellular NS1 protein and promoting the production of infectious DTMUV progeny. CCT2 contributes to the homeostasis of NS1 protein.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Oliguric IRIS Grade V Acute Kidney Injury Associated with Suspected Leptospirosis in an Urban Dog Unvaccinated Against Leptospirosis from the Republic of Korea: A Case Report.","authors":"Taehyun Roh, Kunho Song","doi":"10.3390/vetsci13090982","DOIUrl":"10.3390/vetsci13090982","url":null,"abstract":"<p><p>Leptospirosis is a zoonotic disease that can cause severe acute kidney injury (AKI) in dogs. A three-year-old intact male Border Collie, unvaccinated against leptospirosis, with a history of exposure to urban stagnant water presented with acute vomiting, diarrhea, and moderate dehydration. Initial evaluation revealed extreme azotemia (blood urea nitrogen, 175.8 mg/dL; creatinine, 11.10 mg/dL) and hyperphosphatemia (13.6 mg/dL), consistent with International Renal Interest Society (IRIS) AKI Grade V; urine output remained within the non-oliguric range (2.15-3.0 mL/kg/h) throughout the catheterized period (Day 0-4). Clinical and imaging findings supported acute intrinsic renal injury over chronic kidney disease. A multiplex real-time PCR targeting the <i>Leptospira LipL32</i> gene was positive in urine (Ct 31.10), providing molecular evidence supporting active infection or shedding of pathogenic <i>Leptospira</i> spp., without identifying the infecting serovar. Empirical antimicrobial therapy targeting suspected leptospirosis, together with intravenous fluid therapy, was initiated on clinical suspicion prior to the PCR result. Renal and inflammatory biomarkers improved progressively over 22 days without extracorporeal kidney support; the patient's non-oliguric status, hemodynamic stability, and objective clinical improvement supported this management approach. This report documents, in detail, the clinicopathological recovery trajectory of a dog with non-oliguric IRIS Grade V AKI associated with suspected leptospirosis, information that may assist clinicians in recognizing and managing similarly severe presentations without assuming that dialysis is always required.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resolution of Regenerative Anemia, Thrombocytopenia, and Syncope After Cytoreduction of an Infiltrative Angiolipoma in a Dog: A Case Report.","authors":"Jaeyong Chung, Tae-Seong Moon, Hwi-Yool Kim","doi":"10.3390/vetsci13090975","DOIUrl":"10.3390/vetsci13090975","url":null,"abstract":"<p><p>Angiolipomas are uncommon benign mesenchymal neoplasms composed of mature adipocytes and proliferating vascular channels. In dogs, infiltrative angiolipomas are rarely reported and have primarily been described as localized soft-tissue masses. An 11-year-old, 10.8 kg, castrated male Japanese Spitz was referred for evaluation of regenerative anemia, mild thrombocytopenia, recurrent syncopal episodes, and a 5-year history of a slowly enlarging ventral thoracic mass. Preoperative hematological evaluation demonstrated anemia with a hematocrit of 25.4% and an increased absolute reticulocyte count of 122.7 K/µL, supporting a regenerative erythropoietic response. The platelet count was mildly decreased at 109 × 10³/µL. D-dimer concentration was increased (737.61 ng/mL; reference interval, 0-250 ng/mL), whereas prothrombin time (9.5 s; reference interval, 5-15 s) and activated partial thromboplastin time (15.6 s; reference interval, 15-45 s) were within their reference intervals. Referral history indicated that previous infectious and immune-mediated evaluations had not identified a definitive cause, although the original external testing records were unavailable for verification. Computed tomography revealed a heterogeneous subcutaneous mass with mixed fat- and soft-tissue-attenuating components, suspected infiltration of the superficial pectoral muscle, and focal rather than diffuse contrast enhancement. Surgical cytoreduction, including partial myectomy, was performed because complete excision was not considered feasible owing to deep muscular infiltration. Histopathological examination confirmed an infiltrative angiolipoma characterized by mature adipocytes, numerous vascular channels, skeletal muscle infiltration, and frequent intravascular fibrin thrombi. Following surgery, the hematological abnormalities progressively improved without blood transfusion or long-term immunosuppressive therapy. The platelet count increased from 109 × 10³/µL preoperatively to 153 × 10³/µL by postoperative day 16 and 202 × 10³/µL by postoperative day 56. During the same period, the hematocrit increased from 25.4% to 45.0%, and the absolute reticulocyte count subsequently returned to the reference interval. The syncopal episodes ceased after surgery and did not recur during the available 12-month follow-up. Fibrinogen concentration and peripheral blood smear evaluation were unavailable, and serial postoperative coagulation testing was not performed. Accordingly, the temporal association between tumor cytoreduction and hematological improvement raises the possibility of a tumor-associated process, but neither the underlying mechanism nor a causal relationship could be established.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13611939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection and Phylogenetic Characterization of CPV-2c in Apparently Healthy Domestic Cats in the Guadalajara Metropolitan Area, Mexico.","authors":"Sandra Del Carmen Robles Gil-Bernal, Darwin Elizondo-Quiroga, Jeannette Barba-León, Claudia Lisette Charles-Niño, Ana Samatzin Leal-Pimentel, Jorge Gaona Bernal, César Pedroza-Roldán","doi":"10.3390/vetsci13090977","DOIUrl":"10.3390/vetsci13090977","url":null,"abstract":"<p><p>Carnivore protoparvovirus 1 variants (CPV-2a, CPV-2b, CPV-2c) have progressively expanded their host range to domestic cats (Felis catus), causing subclinical infection or panleukopenia-like disease. CPV-2c dominates among dogs in Western Mexico, but its presence in the local feline population had not been investigated. A cross-sectional survey was conducted (2018-2022) in 210 apparently healthy domestic cats from five municipalities of the Guadalajara Metropolitan Area, Jalisco, Mexico. Genomic DNA from EDTA-anticoagulated blood was screened by generic protoparvovirus PCR; positive samples were characterized by CPV-2-specific VP2 PCR and Sanger sequencing, with variant classification based on VP2 residue 426, and phylogenetic relationships assessed by maximum-likelihood analysis (T92+I model; 1000 bootstrap replicates). Carnivore protoparvovirus 1 DNA was detected in 14/210 cats (6.7%); 13/14 sequenced isolates were classified as CPV-2c (Glu426, >99% nucleotide identity), clustering with regional canine strains and showing greatest affinity with Uruguayan isolates. In a Firth bias-reduced multivariable model adjusting for age and vaccination status, shared litter box use remained independently associated with detection (adjusted OR 18.03; 95% CI 3.30-98.58); the age association was attenuated after adjustment, suggesting incomplete vaccination, rather than age itself, as the more proximal risk factor. Free-roaming/outdoor access and hunting behavior showed direct crude associations with detection (OR 3.82 and 7.22, respectively). These findings show that cats can harbor CPV-2c DNA in blood without clinical signs, phylogenetically linked to local canine strains, underscoring the need for integrated CPV-2 surveillance across companion species; however, whether infected cats can transmit the virus remains undetermined.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"13 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}