Veterinary Sciences最新文献

{"title":"Synthesis of <i>Giardia</i> Species and Genotypes in Wild Birds: A Review.","authors":"Diana Echeverry, Pablo Oyarzún-Ruiz, Carlos Landaeta-Aqueveque","doi":"10.3390/vetsci12090911","DOIUrl":"10.3390/vetsci12090911","url":null,"abstract":"<p><p>Globally, <i>Giardia</i> infections affect an estimated 280 million people each year, making it a significant cause of enteric diarrheal disease. Despite being categorized as a neglected disease, the prevalence of <i>Giardia</i> in wild animals varies, with both mammals and birds serving as hosts. Molecular tools have identified nine <i>Giardia</i> species, including <i>Giardia duodenalis</i> with eight genotypes. Birds, with their wide-ranging habitats and movement capabilities, are crucial disseminators of <i>Giardia</i>. This review aimed to identify <i>Giardia</i> species in wild birds globally, utilizing various academic databases with search terms such as \"wild birds\" and \"<i>Giardia</i>\" in combination with Boolean operators for comprehensive results. <i>Giardia</i> spp. have been identified in thirteen orders of wild birds; however, they have been genotyped in only seven orders. <i>Giardia duodenalis</i> assemblage A was identified in wild birds from the Americas; meanwhile, assemblages A, B, D, and F were identified from Europe, and B and E from Asia. Assemblage B is mainly associated with humans and a wide number of wild animals. Therefore, ascertaining the prevalence of <i>G. duodenalis</i> and its assemblages across diverse avian species and orders is crucial to assess the potential risk of zoonotic transmission associated with the dissemination of this protozoan.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Perspectives and Practices of Pet Owners in Türkiye on Animal Care, Nutrition, and Welfare.","authors":"Salih Çelik, Habip Muruz, Seda Çelik, Mehmet Ferit Can, Mehmet Çelik","doi":"10.3390/vetsci12090904","DOIUrl":"10.3390/vetsci12090904","url":null,"abstract":"<p><p>Although pet ownership is rapidly increasing in developing countries like Turkey, information on how animals are cared for and fed and on perceived animal welfare remains limited. To address this gap, a survey was conducted with 410 participants from 65 provinces, representing over 80% of the country. The study collected data on pet owner demographics, care and feeding practices, and awareness of animal welfare. The results show that most participants (80.6%) have at least a bachelor's degree, and most pets (80.9%) were acquired within the last 10 years. Monthly spending on pet care typically ranges from USD 30 to 90. Ingredient quality emerged as the primary factor driving food choices (51%), driven by a growing interest in premium and super-premium products aimed at improving health and well-being. Veterinary clinics play a significant role in shaping feeding decisions. Most pet owners consider their pets family members and feel quite knowledgeable about their welfare and nutrition. The results suggest that recent trends in human nutrition, such as increasing interest in functional foods and higher ingredient standards, are also shaping pet feeding practices, and that closer collaboration between veterinarians and the pet food industry is needed.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects and Microbiota Changes Following Oral Lyophilized Fecal Microbiota Transplantation Capsules in Canine with Chronic Enteropathy After Parvovirus Infection: Case Report.","authors":"Siyu Liu, Baihui Zhou, Lei Liu, Jialai Zhong, Xinyan Zhang, Wenting Jiang, Haifeng Liu, Ziyao Zhou, Guangneng Peng, Yalin Zhong, Kun Zhang, Zhijun Zhong","doi":"10.3390/vetsci12090909","DOIUrl":"10.3390/vetsci12090909","url":null,"abstract":"<p><p>(1) Background: Chronic enteropathy (CE) in canines is associated with persistent microbiome dysbiosis, and conventional therapies (e.g., special diets, antimicrobials, and immunosuppressive drugs) are sometimes ineffective. Currently, fecal microbiota transplantation (FMT) has proven successful in treating CE in canines via invasive methods (e.g., enemas or endoscopy) or via oral frozen liquid capsules, which must be stored at -80 °C. However, due to the invasiveness of the administration methods and the storage constraints of the liquid capsules, FMT is not widely used in veterinary clinical practice. (2) Methods: The case of a four-year-old Siberian Husky with a three-year history of CE following canine parvovirus infection received lyophilized FMT capsules for thirty days. Stool samples were collected for metagenomic sequencing and quantification of fecal short-chain fatty acids (SCFAs), both pre- and post-FMT. Blood samples were analyzed using complete blood count (CBC) and biochemical testing. Ultrasound was used to assess the wall thickness of the stomach, duodenum, jejunum, and colon. (3) Results: Post-FMT, improvements in clinical outcomes were observed: fecal scores improved from 6 (unformed stools with mucus) to 2 (formed stool), and body weight increased by 8.3% (from 24.2 kg to 26.2 kg). Abnormal CBC and biochemical parameters were restored to reference ranges, including hematocrit (from 60.6% to 55.7%), hemoglobin (from 208 g/L to 190 g/L), creatinine (from 167 μmol/L to 121 μmol/L), and urea (from 11.9 mmol/L to 7.1 mmol/L). Ultrasound results showed that colonic wall thickness decreased from 0.23 ± 0.03 cm (pathological) to 0.18 ± 0.01 cm (physiological). Metagenomic analysis revealed that microbial richness (operational taxonomic units (OTUs) from 151 to 183) and diversity (Shannon and Simpson indices from 3.16 to 4.8 and from 0.87 to 0.94, respectively) all increased. The microbiota composition of the recipient exhibited a decline in the relative abundance of Firmicutes, falling from 99.84% to 35.62%, concomitant with an increase in Actinobacteria (from 0.08% to 4.78%), indicating a convergence toward a donor-like profile. Fecal SCFAs analysis revealed a 251.4% increase in propionate (from 0.0833 to 0.2929 mg/g) and elevated acetate (from 0.4425 to 0.4676 mg/g). These changes are functionally linked to enriched propanoate metabolism (Z = 0.89) in KEGG pathways. (4) Conclusions: Oral lyophilized FMT capsules resolved clinical signs of CE, enhanced microbial diversity and richness, and restored donor-like abundances of gut microbiota, particularly SCFA-producing taxa. Microbial restructuring increased microbial metabolite output, notably SCFA concentrations, and enriched functional metabolic pathways. Importantly, lyophilized FMT overcomes storage limitations and administration barriers, demonstrating its high clinical viability for treating canine CE.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of Equine Lymph Node Endothelial Cells.","authors":"Tomas Lugo, Stephanie Myers, Thu Annelise Nguyen","doi":"10.3390/vetsci12090905","DOIUrl":"10.3390/vetsci12090905","url":null,"abstract":"<p><p>In vitro models have revolutionized our understanding of biological pathways and mechanisms, offering a viable alternative to direct patient testing. However, there is a significant lack of models for different animals, particularly equine models. This study presents a novel primary cell culture extracted from a 3-year-old horse diagnosed with multisystemic eosinophilic epitheliotropic disease. Tissue samples were collected from lymph nodes at various locations. Growth curves of extracted primary cells were analyzed and the optimal conditions were assessed. Biomarkers, such as CD31, ZO-1, CD79, Beta-catenin, E-cadherin, and LYE-1, were detected using an immunofluorescence assay, indicating that these primary cells are of endothelial origin. Initial whole-genome sequencing was performed to confirm the species' origin and to identify the number of common variations in comparison with the NIH EquCab3.0 reference genome. For the first time, the establishment of primary equine cells from lymph nodes is reported, and these can be used as an in vitro model for testing drug responses, molecular pathways, and environmental effects.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Lightweight Dairy Cattle Body Condition Scoring Model for Edge Devices Based on Tail Features and Attention Mechanisms.","authors":"Fan Liu, Yongan Zhang, Yanqiu Liu, Jia Li, Meian Li, Jianping Yao","doi":"10.3390/vetsci12090906","DOIUrl":"10.3390/vetsci12090906","url":null,"abstract":"<p><p>The Body Condition Score (BCS) is a key indicator of dairy cattle's health, production efficiency, and environmental impact. Manual BCS assessment is subjective and time-consuming, limiting its scalability in precision agriculture. This study utilizes computer vision to automatically assess cattle body condition by analyzing tail features, categorizing BCS into five levels (3.25, 3.50, 3.75, 4.0, 4.25). SE attention improves feature selection by adjusting channel importance, while spatial attention enhances spatial information processing by focusing on key image regions. EfficientNet-B0, enhanced by SE and spatial attention mechanisms, improves feature extraction and localization. To facilitate edge device deployment, model distillation reduces the size from 23.8 MB to 8.7 MB, improving inference speed and storage efficiency. After distillation, the model achieved 91.10% accuracy, 91.14% precision, 91.10% recall, and 91.10% F1 score. The accuracy increased to 97.57% for ±0.25 BCS error and 99.72% for ±0.5 error. This model saves space and meets real-time monitoring requirements, making it suitable for edge devices with limited resources. This research provides an efficient, scalable method for automated livestock health monitoring, supporting intelligent animal husbandry development.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attitudes and Preferences of Cat and Dog Owners Towards Pet Food Quality Attributes in Türkiye.","authors":"Onur Erzurum, Tamer Kayar","doi":"10.3390/vetsci12090907","DOIUrl":"10.3390/vetsci12090907","url":null,"abstract":"<p><p>In recent years, pet owners have been making significant efforts to ensure the well-being of their animals. One straightforward approach to enhance their welfare involves greater attention to nutrition. Consequently, pet owners have become increasingly sensitive regarding the food they provide for their pets. The aim of this study is to examine this sensitivity by investigating the extent to which cat and dog owners prioritize quality attributes and ingredients when selecting pet food. For this purpose, a survey was conducted with pet owners (<i>n</i> = 519) who visited veterinary clinics, using a random sampling method. Of the participants, 51.25% were male and 48.75% were female, with the highest participation observed in the 18-34 age group (60.50%). Among the respondents, 64.93% owned cats, while 35.07% owned dogs. Additionally, 66.67% of pet owners reported using both wet and dry food. When selecting pet food, 42.58% of participants stated that they relied on recommendations from veterinarians. \"Pet preference (palatability)\" clearly emerged as the most important selection criterion, receiving the highest average score (3.90) and highlighting its decisive influence on purchasing decisions as owners appeared to prioritize what their pets liked most. In contrast, \"visual appeal of the food\" received the lowest score (2.47). A significant difference (<i>p</i> < 0.001) was found regarding whose recommendation influenced the purchase decision. Moreover, a negative correlation was identified between price sensitivity and other selection criteria, suggesting that price was not a primary driver in most participants' decisions and became less important as quality-related factors gained priority. Overall, pet preference (palatability) stood out as the dominant driver of purchasing decisions.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Antibacterial Activity of Ethanolic Extracts Obtained from Plants Grown in Tolima, Colombia, Against Bacteria Associated with Bovine Mastitis.","authors":"Yeimy Lorena Robledo-Díaz, Aurora Alejandra Sánchez-Varón, Yeli Camila Van-Arcken Aguilar, María Del Pilar Sánchez-Bonilla, Jorge Enrique Hernández-Carvajal","doi":"10.3390/vetsci12090903","DOIUrl":"10.3390/vetsci12090903","url":null,"abstract":"<p><p>Among the main diseases affecting dairy cattle is mastitis, a pathology widely recognized worldwide for causing considerable economic losses for both producers and the dairy industry. The conventional treatment involves the use of antibiotics, for which bacterial resistance has been reported. This fact has created the need to propose alternative treatments for this disease. Among the bacterial microorganisms associated with bovine mastitis are <i>Streptococcus</i> spp. and coagulase-positive <i>Staphylococcus</i>, which were isolated from milk obtained from cattle with mastitis in different dairy farms in the sector of Anaime in Cajamarca, Tolima. The objective of this research was to provide information on the antibacterial activity, toxicity, and phytochemical study (by TLC) of the following five plants-<i>Calendula officinalis</i> L., <i>Psidium guajava</i> L., <i>Matricaria chamomilla</i> L., <i>Rosmarinus officinalis</i> L., and <i>Carica papaya</i> L.-cultivated in Tolima, Colombia, with ethnopharmacological information in the treatment of diseases of bacterial origin. The ethanolic extracts of the selected species were obtained by maceration and were characterized for the presence of flavonoids by TLC. The antibacterial activity was evaluated in vitro using the Kirby-Bauer disk diffusion technique in Mueller-Hinton agar against <i>Streptococcus</i> spp. and coagulase-positive <i>Staphylococcus</i> strains. Ethanolic extracts of <i>Psidium guajava</i> L. (21 ± 3.2) and <i>Rosmarinus officinalis</i> L. (19 ± 2.1) showed the best activity against coagulase-positive <i>Staphylococcus</i>. In addition, ethanolic extracts of <i>Calendula officinalis</i> L. (21 ± 1.9), <i>Rosmarinus officinalis</i> L. (17 ± 2.9 mm), and <i>Psidium guajava</i> L. (15 ± 2.3) were the most active against <i>Streptococcus</i> spp. In contrast, the ethanolic extract of <i>Matricaria chamomilla</i> L. showed no activity against the strains evaluated. All extracts showed toxicity against <i>Artemia salina</i> nauplii at 24 h. It is important to mention that flavonoids were detected using TLC in all the extracts, which may be associated with antibacterial activity.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Hemoglobin, Albumin, Lymphocyte, and Platelet Score as a Prognostic Indicator for Dogs with Congestive Heart Failure Secondary to Myxomatous Mitral Valve Disease.","authors":"Jayeon Park, Yeon Chae, Sungjae Lee, Yoonhoi Koo, Hakhyun Kim, Byeong-Teck Kang, Taesik Yun","doi":"10.3390/vetsci12090908","DOIUrl":"10.3390/vetsci12090908","url":null,"abstract":"<p><p>Reliable prognostic indicators for congestive heart failure (CHF) secondary to myxomatous mitral valve disease (MMVD) in dogs are limited. The hemoglobin, albumin, lymphocyte, and platelet (HALP) score, a prognostic marker in humans, has not been evaluated in veterinary medicine. This study aimed to assess the HALP score's utility for predicting short-term mortality in dogs with CHF due to MMVD. This retrospective study included 54 small-breed dogs. The HALP score was calculated as: hemoglobin (g/L) × albumin (g/L) × lymphocytes (/L)/platelets (/L). Six-, nine-month, and one-year mortality were assessed. The HALP score was significantly higher in survivor groups. Receiver operating characteristic (ROC) analysis showed good predictive accuracy for six- and nine-month mortality (area under the curve > 0.7). A cut-off of 11.13 for six-month mortality yielded 44.44% sensitivity and 94.44% specificity. Kaplan-Meier analysis confirmed that a higher HALP score was associated with significantly longer survival. The HALP score appears to be a valuable, novel prognostic indicator for short-term mortality in dogs with CHF due to MMVD.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Study of DTMUV and LPS on Duck Liver Disease.","authors":"Zhenghui Lan, Zhigang Sun, Yi Wang, Huatao Li, Xuejing Sun","doi":"10.3390/vetsci12090900","DOIUrl":"10.3390/vetsci12090900","url":null,"abstract":"<p><p>This study investigated the structural features of adult duck liver and compared pathological alterations induced by duck Tembusu virus (DTMUV, strain XZ-2012) and lipopolysaccharide (LPS). Histological techniques (HE, reticular fiber, and trichrome staining) revealed normal duck liver exhibited reddish-brown coloration with indistinct lobule boundaries and no prominent bile ducts. Kupffer cell distribution was mapped via jugular ink injection. DTMUV infection caused liver swelling, congestion, and yellowish discoloration. Histopathology showed lymphocyte infiltration around central veins and portal areas, increased reticular fibers, thickened basement membranes, hepatocyte vacuolation, and erythrocyte accumulation in sinusoids. In contrast, LPS exposure led to mild hepatic enlargement without vacuolar degeneration but with marked perivascular lymphocyte aggregation and reticular fiber proliferation. Both treatments elevated Kupffer cell numbers. These findings demonstrate distinct liver injury patterns: DTMUV induces direct hepatocellular damage with inflammatory responses, while LPS triggers intense immune cell recruitment without significant hepatocyte degeneration. The study provides insights into avian viral versus bacterial pathogenesis and liver defense mechanisms, offering a foundation for further research into waterfowl infectious diseases.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Study of <i>Theileria annulata</i> and <i>Anaplasma</i> spp. in Ixodid Ticks from Southern Regions of the Republic of Kazakhstan.","authors":"Zaure Z Sayakova, Saltanat A Kenessary, Ainur A Zhaksylykova, Bagzhan M Abdimalik, Eleonora A Kydyrkhanova, Dinara K Kamalova, Anara Ryskeldina, Yekaterina O Ostapchuk, Christine M Budke, Aida M Abdybekova","doi":"10.3390/vetsci12090901","DOIUrl":"10.3390/vetsci12090901","url":null,"abstract":"<p><p>Ixodid ticks are vectors of pathogens that cause dangerous infectious and parasitic diseases in animals, leading to reduced productivity and, in some cases, mass mortality. In Kazakhstan, information on tick fauna and their epizootological role in the transmission of hemoparasites is limited. This study aimed to determine the species composition of ixodid ticks parasitizing cattle in the Almaty, Zhambyl, and Turkistan oblasts, and to assess their potential role in the transmission of <i>Theileria</i> and <i>Anaplasma</i> spp. A total of 3121 ixodid ticks were collected from cattle, belonging to the genera <i>Hyalomma</i> (86.9%; 2711/3121), <i>Rhipicephalus</i> (8.5%; 266/3121), <i>Dermacentor</i> (3.2%; 101/3121), and <i>Haemaphysalis</i> (1.4%; 43/3121). Morphological identification revealed that ticks of the genus <i>Hyalomma</i>, including <i>Hyalomma scupense</i> (31.7%), <i>Hyalomma asiaticum</i> (27.9%), and <i>Hyalomma anatolicum</i> (19.6%), were the predominant species. <i>Rhipicephalus annulatus</i> was recorded for the first time in the Almaty and Zhambyl oblasts. Partial sequencing of the <i>cox1</i> gene confirmed the species identification obtained by morphological examination. A total of 113 representative ticks were subjected to DNA extraction for the identification of <i>Theileria</i> and <i>Anaplasma</i> species using conventional PCR with primers targeting the <i>28SrRNA</i> and <i>GroEL</i> genes, respectively. No <i>Anaplasma</i> spp. were detected in the analyzed tick samples. <i>Theileria annulata</i> DNA was identified in seven nymphs of <i>Hy. scupense</i> (6.1%) collected in the Zhambyl oblast, and for the first time in Kazakhstan, in one female <i>Rhipicephalus annulatus</i> (0.9%) collected in the Almaty oblast. The overall infection prevalence of <i>Theileria annulata</i> was 7.0% (8/113).</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}