马淋巴结内皮细胞的分离与鉴定。

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Tomas Lugo, Stephanie Myers, Thu Annelise Nguyen
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引用次数: 0

摘要

体外模型彻底改变了我们对生物学途径和机制的理解,为直接患者测试提供了可行的替代方案。然而,目前严重缺乏不同动物的模型,尤其是马的模型。本研究提出了一种新的原代细胞培养物,提取自一匹被诊断为多系统嗜酸性上皮性疾病的3岁马。从不同部位的淋巴结收集组织样本。对提取的原代细胞生长曲线进行分析,并对最佳条件进行评价。生物标志物,如CD31、ZO-1、CD79、β -catenin、E-cadherin和LYE-1,用免疫荧光法检测,表明这些原代细胞是内皮细胞。进行了初始全基因组测序,以确认物种的起源,并与NIH EquCab3.0参考基因组进行比较,确定常见变异的数量。本文首次报道了马淋巴结原代细胞的建立,这些细胞可以作为体外模型来测试药物反应、分子途径和环境效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and Characterization of Equine Lymph Node Endothelial Cells.

In vitro models have revolutionized our understanding of biological pathways and mechanisms, offering a viable alternative to direct patient testing. However, there is a significant lack of models for different animals, particularly equine models. This study presents a novel primary cell culture extracted from a 3-year-old horse diagnosed with multisystemic eosinophilic epitheliotropic disease. Tissue samples were collected from lymph nodes at various locations. Growth curves of extracted primary cells were analyzed and the optimal conditions were assessed. Biomarkers, such as CD31, ZO-1, CD79, Beta-catenin, E-cadherin, and LYE-1, were detected using an immunofluorescence assay, indicating that these primary cells are of endothelial origin. Initial whole-genome sequencing was performed to confirm the species' origin and to identify the number of common variations in comparison with the NIH EquCab3.0 reference genome. For the first time, the establishment of primary equine cells from lymph nodes is reported, and these can be used as an in vitro model for testing drug responses, molecular pathways, and environmental effects.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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