口服冻干粪便菌群移植胶囊对细小病毒感染后慢性肠病犬的影响和菌群变化:一例报告。

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Siyu Liu, Baihui Zhou, Lei Liu, Jialai Zhong, Xinyan Zhang, Wenting Jiang, Haifeng Liu, Ziyao Zhou, Guangneng Peng, Yalin Zhong, Kun Zhang, Zhijun Zhong
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引用次数: 0

摘要

(1)背景:犬的慢性肠病(CE)与持续的微生物群失调有关,常规治疗(如特殊饮食、抗菌剂和免疫抑制药物)有时是无效的。目前,粪便微生物群移植(FMT)已被证明可以通过侵入性方法(例如灌肠或内窥镜检查)或口服冷冻液体胶囊(必须在-80°C下储存)成功治疗犬CE。然而,由于给药方法的侵入性和液体胶囊的储存限制,FMT并未广泛应用于兽医临床实践。(2)方法:犬细小病毒感染后出现3年CE病史的4岁西伯利亚哈士奇,给予冻干FMT胶囊30天。收集粪便样本,对fmt前后的粪便短链脂肪酸(SCFAs)进行宏基因组测序和定量。血液样本采用全血细胞计数(CBC)和生化测试进行分析。超声检查胃、十二指肠、空肠、结肠的壁厚。(3)结果:fmt后,临床结果有所改善:粪便评分从6分(未形成粪便带粘液)改善到2分(形成粪便),体重增加8.3%(从24.2 kg增加到26.2 kg)。血球比容(从60.6%到55.7%)、血红蛋白(从208 g/L到190 g/L)、肌酐(从167 μmol/L到121 μmol/L)、尿素(从11.9 mmol/L到7.1 mmol/L)等生化指标恢复到参考范围。超声结果显示,结肠壁厚度由病理值0.23±0.03 cm降至生理值0.18±0.01 cm。宏基因组分析显示,微生物丰富度(OTUs从151增加到183)和多样性(Shannon和Simpson指数分别从3.16增加到4.8和0.87增加到0.94)均有所增加。受者的微生物群组成显示厚壁菌门的相对丰度下降,从99.84%下降到35.62%,同时放线菌门的增加(从0.08%增加到4.78%),表明向供者样的特征趋同。粪便SCFAs分析显示丙酸增加251.4%(从0.0833 mg/g增加到0.2929 mg/g),乙酸增加(从0.4425 mg/g增加到0.4676 mg/g)。这些变化在功能上与KEGG通路中丰富的丙酸代谢(Z = 0.89)有关。(4)结论:口服冻干FMT胶囊解决了CE的临床症状,增强了微生物的多样性和丰富度,恢复了供体样肠道微生物群的丰度,特别是产生scfa的分类群。微生物重组增加了微生物代谢物的输出,特别是SCFA浓度,并丰富了功能性代谢途径。重要的是,冻干FMT克服了储存限制和给药障碍,表明其在治疗犬CE方面具有很高的临床可行性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects and Microbiota Changes Following Oral Lyophilized Fecal Microbiota Transplantation Capsules in Canine with Chronic Enteropathy After Parvovirus Infection: Case Report.

(1) Background: Chronic enteropathy (CE) in canines is associated with persistent microbiome dysbiosis, and conventional therapies (e.g., special diets, antimicrobials, and immunosuppressive drugs) are sometimes ineffective. Currently, fecal microbiota transplantation (FMT) has proven successful in treating CE in canines via invasive methods (e.g., enemas or endoscopy) or via oral frozen liquid capsules, which must be stored at -80 °C. However, due to the invasiveness of the administration methods and the storage constraints of the liquid capsules, FMT is not widely used in veterinary clinical practice. (2) Methods: The case of a four-year-old Siberian Husky with a three-year history of CE following canine parvovirus infection received lyophilized FMT capsules for thirty days. Stool samples were collected for metagenomic sequencing and quantification of fecal short-chain fatty acids (SCFAs), both pre- and post-FMT. Blood samples were analyzed using complete blood count (CBC) and biochemical testing. Ultrasound was used to assess the wall thickness of the stomach, duodenum, jejunum, and colon. (3) Results: Post-FMT, improvements in clinical outcomes were observed: fecal scores improved from 6 (unformed stools with mucus) to 2 (formed stool), and body weight increased by 8.3% (from 24.2 kg to 26.2 kg). Abnormal CBC and biochemical parameters were restored to reference ranges, including hematocrit (from 60.6% to 55.7%), hemoglobin (from 208 g/L to 190 g/L), creatinine (from 167 μmol/L to 121 μmol/L), and urea (from 11.9 mmol/L to 7.1 mmol/L). Ultrasound results showed that colonic wall thickness decreased from 0.23 ± 0.03 cm (pathological) to 0.18 ± 0.01 cm (physiological). Metagenomic analysis revealed that microbial richness (operational taxonomic units (OTUs) from 151 to 183) and diversity (Shannon and Simpson indices from 3.16 to 4.8 and from 0.87 to 0.94, respectively) all increased. The microbiota composition of the recipient exhibited a decline in the relative abundance of Firmicutes, falling from 99.84% to 35.62%, concomitant with an increase in Actinobacteria (from 0.08% to 4.78%), indicating a convergence toward a donor-like profile. Fecal SCFAs analysis revealed a 251.4% increase in propionate (from 0.0833 to 0.2929 mg/g) and elevated acetate (from 0.4425 to 0.4676 mg/g). These changes are functionally linked to enriched propanoate metabolism (Z = 0.89) in KEGG pathways. (4) Conclusions: Oral lyophilized FMT capsules resolved clinical signs of CE, enhanced microbial diversity and richness, and restored donor-like abundances of gut microbiota, particularly SCFA-producing taxa. Microbial restructuring increased microbial metabolite output, notably SCFA concentrations, and enriched functional metabolic pathways. Importantly, lyophilized FMT overcomes storage limitations and administration barriers, demonstrating its high clinical viability for treating canine CE.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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