DTMUV与LPS对鸭肝脏疾病的比较研究。

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Zhenghui Lan, Zhigang Sun, Yi Wang, Huatao Li, Xuejing Sun
{"title":"DTMUV与LPS对鸭肝脏疾病的比较研究。","authors":"Zhenghui Lan, Zhigang Sun, Yi Wang, Huatao Li, Xuejing Sun","doi":"10.3390/vetsci12090900","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigated the structural features of adult duck liver and compared pathological alterations induced by duck Tembusu virus (DTMUV, strain XZ-2012) and lipopolysaccharide (LPS). Histological techniques (HE, reticular fiber, and trichrome staining) revealed normal duck liver exhibited reddish-brown coloration with indistinct lobule boundaries and no prominent bile ducts. Kupffer cell distribution was mapped via jugular ink injection. DTMUV infection caused liver swelling, congestion, and yellowish discoloration. Histopathology showed lymphocyte infiltration around central veins and portal areas, increased reticular fibers, thickened basement membranes, hepatocyte vacuolation, and erythrocyte accumulation in sinusoids. In contrast, LPS exposure led to mild hepatic enlargement without vacuolar degeneration but with marked perivascular lymphocyte aggregation and reticular fiber proliferation. Both treatments elevated Kupffer cell numbers. These findings demonstrate distinct liver injury patterns: DTMUV induces direct hepatocellular damage with inflammatory responses, while LPS triggers intense immune cell recruitment without significant hepatocyte degeneration. The study provides insights into avian viral versus bacterial pathogenesis and liver defense mechanisms, offering a foundation for further research into waterfowl infectious diseases.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474332/pdf/","citationCount":"0","resultStr":"{\"title\":\"Comparative Study of DTMUV and LPS on Duck Liver Disease.\",\"authors\":\"Zhenghui Lan, Zhigang Sun, Yi Wang, Huatao Li, Xuejing Sun\",\"doi\":\"10.3390/vetsci12090900\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study investigated the structural features of adult duck liver and compared pathological alterations induced by duck Tembusu virus (DTMUV, strain XZ-2012) and lipopolysaccharide (LPS). Histological techniques (HE, reticular fiber, and trichrome staining) revealed normal duck liver exhibited reddish-brown coloration with indistinct lobule boundaries and no prominent bile ducts. Kupffer cell distribution was mapped via jugular ink injection. DTMUV infection caused liver swelling, congestion, and yellowish discoloration. Histopathology showed lymphocyte infiltration around central veins and portal areas, increased reticular fibers, thickened basement membranes, hepatocyte vacuolation, and erythrocyte accumulation in sinusoids. In contrast, LPS exposure led to mild hepatic enlargement without vacuolar degeneration but with marked perivascular lymphocyte aggregation and reticular fiber proliferation. Both treatments elevated Kupffer cell numbers. These findings demonstrate distinct liver injury patterns: DTMUV induces direct hepatocellular damage with inflammatory responses, while LPS triggers intense immune cell recruitment without significant hepatocyte degeneration. The study provides insights into avian viral versus bacterial pathogenesis and liver defense mechanisms, offering a foundation for further research into waterfowl infectious diseases.</p>\",\"PeriodicalId\":23694,\"journal\":{\"name\":\"Veterinary Sciences\",\"volume\":\"12 9\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-09-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474332/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Sciences\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3390/vetsci12090900\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Sciences","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/vetsci12090900","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

本研究研究了成年鸭肝脏的结构特征,并比较了鸭坦布苏病毒(DTMUV, XZ-2012)和脂多糖(LPS)对成年鸭肝脏的病理改变。组织学技术(HE,网状纤维和三色染色)显示正常鸭肝脏呈红褐色,小叶边界不清,胆管不突出。通过颈静脉注射墨水绘制库普弗细胞分布图。DTMUV感染引起肝脏肿胀、充血和变黄。组织病理学显示中央静脉和门静脉周围淋巴细胞浸润,网状纤维增多,基底膜增厚,肝细胞空泡化,血窦内红细胞积聚。相比之下,LPS暴露导致肝脏轻度增大,无空泡变性,但血管周围淋巴细胞聚集和网状纤维增生明显。两种治疗方法都提高了库普弗细胞的数量。这些发现证明了不同的肝损伤模式:DTMUV诱导直接肝细胞损伤并伴有炎症反应,而LPS触发强烈的免疫细胞募集,但没有明显的肝细胞变性。该研究为进一步了解禽病毒对细菌的致病机制和肝脏防御机制提供了新的思路,为进一步研究水禽传染病奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Study of DTMUV and LPS on Duck Liver Disease.

This study investigated the structural features of adult duck liver and compared pathological alterations induced by duck Tembusu virus (DTMUV, strain XZ-2012) and lipopolysaccharide (LPS). Histological techniques (HE, reticular fiber, and trichrome staining) revealed normal duck liver exhibited reddish-brown coloration with indistinct lobule boundaries and no prominent bile ducts. Kupffer cell distribution was mapped via jugular ink injection. DTMUV infection caused liver swelling, congestion, and yellowish discoloration. Histopathology showed lymphocyte infiltration around central veins and portal areas, increased reticular fibers, thickened basement membranes, hepatocyte vacuolation, and erythrocyte accumulation in sinusoids. In contrast, LPS exposure led to mild hepatic enlargement without vacuolar degeneration but with marked perivascular lymphocyte aggregation and reticular fiber proliferation. Both treatments elevated Kupffer cell numbers. These findings demonstrate distinct liver injury patterns: DTMUV induces direct hepatocellular damage with inflammatory responses, while LPS triggers intense immune cell recruitment without significant hepatocyte degeneration. The study provides insights into avian viral versus bacterial pathogenesis and liver defense mechanisms, offering a foundation for further research into waterfowl infectious diseases.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信