哈萨克斯坦共和国南部地区伊蚊环芽孢杆菌和无原体的分子研究。

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Zaure Z Sayakova, Saltanat A Kenessary, Ainur A Zhaksylykova, Bagzhan M Abdimalik, Eleonora A Kydyrkhanova, Dinara K Kamalova, Anara Ryskeldina, Yekaterina O Ostapchuk, Christine M Budke, Aida M Abdybekova
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引用次数: 0

摘要

粘蜱是病原体的载体,在动物中引起危险的传染病和寄生虫病,导致生产力下降,在某些情况下导致大量死亡。在哈萨克斯坦,关于蜱动物群及其在血液寄生虫传播中的流行病学作用的信息有限。本研究旨在了解阿拉木图、樟比勒和突厥斯坦三州牛体中蜱的种类组成,并探讨其在牛体中传播蜱属和无原体的作用。共采集牛体中蜱3121只,隶属于透明蜱属(86.9%;2711/3121)、鼻头蜱属(8.5%;266/3121)、皮蜱属(3.2%;101/3121)和血蜱属(1.4%;43/3121)。形态鉴定结果显示,蜱属优势种为细纹蜱(31.7%)、亚洲蜱(27.9%)和斑点蜱(19.6%)。在阿拉木图州和樟白勒州首次记录到环状棘头虫。cox1基因的部分测序证实了形态学检查获得的物种鉴定。对113只代表性蜱进行DNA提取,分别以28SrRNA和GroEL基因为引物,采用常规PCR方法对蜱属和无原体进行鉴定。蜱类标本未检出无原体。在樟比勒州采集的7只海蝇稚虫(6.1%)和阿拉木图州采集的1只环头棘蝇雌虫(0.9%)中首次鉴定出环状菌DNA。环芽孢杆菌总感染率为7.0%(8/113)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Study of Theileria annulata and Anaplasma spp. in Ixodid Ticks from Southern Regions of the Republic of Kazakhstan.

Ixodid ticks are vectors of pathogens that cause dangerous infectious and parasitic diseases in animals, leading to reduced productivity and, in some cases, mass mortality. In Kazakhstan, information on tick fauna and their epizootological role in the transmission of hemoparasites is limited. This study aimed to determine the species composition of ixodid ticks parasitizing cattle in the Almaty, Zhambyl, and Turkistan oblasts, and to assess their potential role in the transmission of Theileria and Anaplasma spp. A total of 3121 ixodid ticks were collected from cattle, belonging to the genera Hyalomma (86.9%; 2711/3121), Rhipicephalus (8.5%; 266/3121), Dermacentor (3.2%; 101/3121), and Haemaphysalis (1.4%; 43/3121). Morphological identification revealed that ticks of the genus Hyalomma, including Hyalomma scupense (31.7%), Hyalomma asiaticum (27.9%), and Hyalomma anatolicum (19.6%), were the predominant species. Rhipicephalus annulatus was recorded for the first time in the Almaty and Zhambyl oblasts. Partial sequencing of the cox1 gene confirmed the species identification obtained by morphological examination. A total of 113 representative ticks were subjected to DNA extraction for the identification of Theileria and Anaplasma species using conventional PCR with primers targeting the 28SrRNA and GroEL genes, respectively. No Anaplasma spp. were detected in the analyzed tick samples. Theileria annulata DNA was identified in seven nymphs of Hy. scupense (6.1%) collected in the Zhambyl oblast, and for the first time in Kazakhstan, in one female Rhipicephalus annulatus (0.9%) collected in the Almaty oblast. The overall infection prevalence of Theileria annulata was 7.0% (8/113).

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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