Zaure Z Sayakova, Saltanat A Kenessary, Ainur A Zhaksylykova, Bagzhan M Abdimalik, Eleonora A Kydyrkhanova, Dinara K Kamalova, Anara Ryskeldina, Yekaterina O Ostapchuk, Christine M Budke, Aida M Abdybekova
{"title":"哈萨克斯坦共和国南部地区伊蚊环芽孢杆菌和无原体的分子研究。","authors":"Zaure Z Sayakova, Saltanat A Kenessary, Ainur A Zhaksylykova, Bagzhan M Abdimalik, Eleonora A Kydyrkhanova, Dinara K Kamalova, Anara Ryskeldina, Yekaterina O Ostapchuk, Christine M Budke, Aida M Abdybekova","doi":"10.3390/vetsci12090901","DOIUrl":null,"url":null,"abstract":"<p><p>Ixodid ticks are vectors of pathogens that cause dangerous infectious and parasitic diseases in animals, leading to reduced productivity and, in some cases, mass mortality. In Kazakhstan, information on tick fauna and their epizootological role in the transmission of hemoparasites is limited. This study aimed to determine the species composition of ixodid ticks parasitizing cattle in the Almaty, Zhambyl, and Turkistan oblasts, and to assess their potential role in the transmission of <i>Theileria</i> and <i>Anaplasma</i> spp. A total of 3121 ixodid ticks were collected from cattle, belonging to the genera <i>Hyalomma</i> (86.9%; 2711/3121), <i>Rhipicephalus</i> (8.5%; 266/3121), <i>Dermacentor</i> (3.2%; 101/3121), and <i>Haemaphysalis</i> (1.4%; 43/3121). Morphological identification revealed that ticks of the genus <i>Hyalomma</i>, including <i>Hyalomma scupense</i> (31.7%), <i>Hyalomma asiaticum</i> (27.9%), and <i>Hyalomma anatolicum</i> (19.6%), were the predominant species. <i>Rhipicephalus annulatus</i> was recorded for the first time in the Almaty and Zhambyl oblasts. Partial sequencing of the <i>cox1</i> gene confirmed the species identification obtained by morphological examination. A total of 113 representative ticks were subjected to DNA extraction for the identification of <i>Theileria</i> and <i>Anaplasma</i> species using conventional PCR with primers targeting the <i>28SrRNA</i> and <i>GroEL</i> genes, respectively. No <i>Anaplasma</i> spp. were detected in the analyzed tick samples. <i>Theileria annulata</i> DNA was identified in seven nymphs of <i>Hy. scupense</i> (6.1%) collected in the Zhambyl oblast, and for the first time in Kazakhstan, in one female <i>Rhipicephalus annulatus</i> (0.9%) collected in the Almaty oblast. The overall infection prevalence of <i>Theileria annulata</i> was 7.0% (8/113).</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474161/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular Study of <i>Theileria annulata</i> and <i>Anaplasma</i> spp. in Ixodid Ticks from Southern Regions of the Republic of Kazakhstan.\",\"authors\":\"Zaure Z Sayakova, Saltanat A Kenessary, Ainur A Zhaksylykova, Bagzhan M Abdimalik, Eleonora A Kydyrkhanova, Dinara K Kamalova, Anara Ryskeldina, Yekaterina O Ostapchuk, Christine M Budke, Aida M Abdybekova\",\"doi\":\"10.3390/vetsci12090901\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Ixodid ticks are vectors of pathogens that cause dangerous infectious and parasitic diseases in animals, leading to reduced productivity and, in some cases, mass mortality. In Kazakhstan, information on tick fauna and their epizootological role in the transmission of hemoparasites is limited. This study aimed to determine the species composition of ixodid ticks parasitizing cattle in the Almaty, Zhambyl, and Turkistan oblasts, and to assess their potential role in the transmission of <i>Theileria</i> and <i>Anaplasma</i> spp. A total of 3121 ixodid ticks were collected from cattle, belonging to the genera <i>Hyalomma</i> (86.9%; 2711/3121), <i>Rhipicephalus</i> (8.5%; 266/3121), <i>Dermacentor</i> (3.2%; 101/3121), and <i>Haemaphysalis</i> (1.4%; 43/3121). Morphological identification revealed that ticks of the genus <i>Hyalomma</i>, including <i>Hyalomma scupense</i> (31.7%), <i>Hyalomma asiaticum</i> (27.9%), and <i>Hyalomma anatolicum</i> (19.6%), were the predominant species. <i>Rhipicephalus annulatus</i> was recorded for the first time in the Almaty and Zhambyl oblasts. Partial sequencing of the <i>cox1</i> gene confirmed the species identification obtained by morphological examination. A total of 113 representative ticks were subjected to DNA extraction for the identification of <i>Theileria</i> and <i>Anaplasma</i> species using conventional PCR with primers targeting the <i>28SrRNA</i> and <i>GroEL</i> genes, respectively. No <i>Anaplasma</i> spp. were detected in the analyzed tick samples. <i>Theileria annulata</i> DNA was identified in seven nymphs of <i>Hy. scupense</i> (6.1%) collected in the Zhambyl oblast, and for the first time in Kazakhstan, in one female <i>Rhipicephalus annulatus</i> (0.9%) collected in the Almaty oblast. 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Molecular Study of Theileria annulata and Anaplasma spp. in Ixodid Ticks from Southern Regions of the Republic of Kazakhstan.
Ixodid ticks are vectors of pathogens that cause dangerous infectious and parasitic diseases in animals, leading to reduced productivity and, in some cases, mass mortality. In Kazakhstan, information on tick fauna and their epizootological role in the transmission of hemoparasites is limited. This study aimed to determine the species composition of ixodid ticks parasitizing cattle in the Almaty, Zhambyl, and Turkistan oblasts, and to assess their potential role in the transmission of Theileria and Anaplasma spp. A total of 3121 ixodid ticks were collected from cattle, belonging to the genera Hyalomma (86.9%; 2711/3121), Rhipicephalus (8.5%; 266/3121), Dermacentor (3.2%; 101/3121), and Haemaphysalis (1.4%; 43/3121). Morphological identification revealed that ticks of the genus Hyalomma, including Hyalomma scupense (31.7%), Hyalomma asiaticum (27.9%), and Hyalomma anatolicum (19.6%), were the predominant species. Rhipicephalus annulatus was recorded for the first time in the Almaty and Zhambyl oblasts. Partial sequencing of the cox1 gene confirmed the species identification obtained by morphological examination. A total of 113 representative ticks were subjected to DNA extraction for the identification of Theileria and Anaplasma species using conventional PCR with primers targeting the 28SrRNA and GroEL genes, respectively. No Anaplasma spp. were detected in the analyzed tick samples. Theileria annulata DNA was identified in seven nymphs of Hy. scupense (6.1%) collected in the Zhambyl oblast, and for the first time in Kazakhstan, in one female Rhipicephalus annulatus (0.9%) collected in the Almaty oblast. The overall infection prevalence of Theileria annulata was 7.0% (8/113).
期刊介绍:
Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.