哥伦比亚Tolima植物乙醇提取物对奶牛乳腺炎相关细菌的体外抗菌活性研究

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Yeimy Lorena Robledo-Díaz, Aurora Alejandra Sánchez-Varón, Yeli Camila Van-Arcken Aguilar, María Del Pilar Sánchez-Bonilla, Jorge Enrique Hernández-Carvajal
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引用次数: 0

摘要

影响奶牛的主要疾病之一是乳腺炎,这是一种全世界公认的疾病,给生产者和乳制品行业造成了相当大的经济损失。传统的治疗方法包括使用抗生素,据报道细菌对抗生素有耐药性。这一事实产生了提出这种疾病的替代治疗方法的需要。在与牛乳腺炎相关的细菌微生物中,有链球菌和凝固酶阳性葡萄球菌,它们是从托利马卡哈马卡Anaime地区不同奶牛场乳腺炎牛的牛奶中分离出来的。本研究的目的是通过薄层色谱(TLC)研究在哥伦比亚Tolima种植的金盏菊(calendula officinalis L.)、瓜石榴金盏花(Psidium guajava L.)、洋甘菊(Matricaria chamomilla L.)、迷迭香(Rosmarinus officinalis L.)和番木瓜(Carica papaya L.)五种植物的抗菌活性、毒性和植物化学研究,以及治疗细菌性疾病的民族药理学信息。采用浸渍法制备乙醇提取物,用薄层色谱法对其黄酮含量进行表征。采用Kirby-Bauer圆盘扩散法测定其对链球菌和凝固酶阳性葡萄球菌的体外抑菌活性。番石榴石蕊醇提物(21±3.2)和迷迭香醇提物(19±2.1)对凝固酶阳性葡萄球菌的抑制活性最好。金盏菊(21±1.9 mm)、迷迭香(17±2.9 mm)和瓜石榴石蕊(15±2.3 mm)乙醇提取物对链球菌的抑制作用最强,而甘菊乙醇提取物对链球菌的抑制作用不明显。所有提取物在24 h时均显示出对盐渍蒿的毒性。值得注意的是,所有提取物的薄层色谱检测到黄酮类化合物,这可能与抗菌活性有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In Vitro Antibacterial Activity of Ethanolic Extracts Obtained from Plants Grown in Tolima, Colombia, Against Bacteria Associated with Bovine Mastitis.

Among the main diseases affecting dairy cattle is mastitis, a pathology widely recognized worldwide for causing considerable economic losses for both producers and the dairy industry. The conventional treatment involves the use of antibiotics, for which bacterial resistance has been reported. This fact has created the need to propose alternative treatments for this disease. Among the bacterial microorganisms associated with bovine mastitis are Streptococcus spp. and coagulase-positive Staphylococcus, which were isolated from milk obtained from cattle with mastitis in different dairy farms in the sector of Anaime in Cajamarca, Tolima. The objective of this research was to provide information on the antibacterial activity, toxicity, and phytochemical study (by TLC) of the following five plants-Calendula officinalis L., Psidium guajava L., Matricaria chamomilla L., Rosmarinus officinalis L., and Carica papaya L.-cultivated in Tolima, Colombia, with ethnopharmacological information in the treatment of diseases of bacterial origin. The ethanolic extracts of the selected species were obtained by maceration and were characterized for the presence of flavonoids by TLC. The antibacterial activity was evaluated in vitro using the Kirby-Bauer disk diffusion technique in Mueller-Hinton agar against Streptococcus spp. and coagulase-positive Staphylococcus strains. Ethanolic extracts of Psidium guajava L. (21 ± 3.2) and Rosmarinus officinalis L. (19 ± 2.1) showed the best activity against coagulase-positive Staphylococcus. In addition, ethanolic extracts of Calendula officinalis L. (21 ± 1.9), Rosmarinus officinalis L. (17 ± 2.9 mm), and Psidium guajava L. (15 ± 2.3) were the most active against Streptococcus spp. In contrast, the ethanolic extract of Matricaria chamomilla L. showed no activity against the strains evaluated. All extracts showed toxicity against Artemia salina nauplii at 24 h. It is important to mention that flavonoids were detected using TLC in all the extracts, which may be associated with antibacterial activity.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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