Veterinary Sciences最新文献

{"title":"Comprehensive Profiling of Essential Elements and Organic and Inorganic Contaminants in Dromedary Camels from the Canary Islands: A Baseline for Nutritional and Environmental Assessment.","authors":"Andrea Acosta-Dacal, Adrián Melián Henríquez, Juan Alberto Corbera, Ana Macías-Montes, Manuel Zumbado, Norberto Ruiz-Suárez, José Luis Martín-Barrasa, Octavio P Luzardo, María Teresa Tejedor-Junco","doi":"10.3390/vetsci12090829","DOIUrl":"10.3390/vetsci12090829","url":null,"abstract":"<p><p>Dromedary camels raised under semi-extensive management can act as One Health sentinels for environmental exposures and food chain surveillance, yet serum reference information remains scarce. Our objective was to provide the most comprehensive assessment to date of physiological and toxicological serum profiles in dromedary camels (<i>Camelus dromedarius</i>) from the Canary Islands. We included 114 clinically healthy animals of different sex, age, and reproductive status. Serum samples were analyzed for essential, toxic, and potentially toxic elements using inductively coupled plasma mass spectrometry (ICP-MS). In addition, a high-throughput multi-residue method based on QuEChERS extraction followed by UHPLC-MS/MS and GC-MS/MS was used to screen for 360 organic compounds, including pesticides, veterinary drugs, human pharmaceuticals, and persistent organic pollutants. Essential elements showed biologically consistent variations according to sex, age group, and pregnancy status. Males had higher levels of selenium and copper, while calves showed elevated concentrations of manganese and zinc. Pregnant females exhibited lower iron, zinc, and selenium levels, consistent with increased fetal demand. These results provide preliminary reference values for healthy camels, stratified by physiological status. In contrast, classical toxic elements such as arsenic, mercury, lead, and cadmium were found at very low or undetectable concentrations. Several potentially toxic elements, including barium, strontium, and rare earth elements, were detected sporadically but without toxicological concern. Only 13 organic compounds (3.6%) were detected in any sample, and concentrations were consistently low. The most prevalent was the PAH acenaphthene (55.3%), followed by the fungicide procymidone and the PAH fluorene. Notably, no residues of the usually detected 4,4'-DDE or PCB congeners were found in any sample. These findings confirm the low environmental and dietary exposure of camels under low-intensity farming systems and highlight their value as sentinel species for food safety and environmental monitoring.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective Uncontrolled Interventional Study of Itraconazole and β-Glucans (<i>Euglena gracilis</i>) to Assess Safeness and Clinical Effectiveness in Cats with Cutaneous and Mucosal Sporotrichosis.","authors":"André Felipe Pedrazzi Chacon, Anna Barreto Fernandes Figueiredo, Jéssica Sepulveda Boechat, Erica Guerino Reis, Cindy Caroline Dos Santos Honorato, Maria Lopes Corrêa, Sandro Antonio Pereira, Isabella Dib Ferreira Gremião","doi":"10.3390/vetsci12090830","DOIUrl":"10.3390/vetsci12090830","url":null,"abstract":"<p><p>Brazil is an endemic region for both human and feline sporotrichosis, with <i>Sporothrix brasiliensis</i> being the main etiological agent. Currently, few effective antifungal agents are available for treating this mycosis in cats, and therapeutic studies remain limited. Itraconazole (ITZ) is the first-line drug; however, its effectiveness is variable. To evaluate the use of ITZ combined with β-glucans (<i>Euglena gracilis</i>) in feline sporotrichosis, a prospective, uncontrolled interventional study was conducted in 29 cats. Clinical cure was achieved in 21 animals (72.4%) with a median treatment duration of 10 weeks. Most of these cats presented with nasal region lesions, nasal mucosa involvement, and respiratory signs, which are commonly associated with poor therapeutic outcomes. Treatment failure occurred in 5 animals (17.2%), and 3 (10.3%) were lost to follow-up. No deaths were recorded during the study. Adverse drug reactions (ADRs) were observed in 2 cats (6.9%). These findings suggest that β-glucans may be a complementary strategy in the treatment of feline sporotrichosis, particularly in cases involving nasal lesions and respiratory signs, and may also contribute to the prevention of ADRs associated with conventional therapy.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Characterization of <i>Cryptococcus neoformans</i> Isolated from Zebra Dove Excreta in Songkhla, Thailand.","authors":"Saowakon Indoung, Sanicha Chumtong, Sakaoporn Prachantasena, Ratchakul Wiriyaprom, Komwit Surachat, Sarunyou Chusri, Rattanaruji Pomwised, Ruttayaporn Ngasaman","doi":"10.3390/vetsci12090827","DOIUrl":"10.3390/vetsci12090827","url":null,"abstract":"<p><p>The research aimed to study the genome of <i>Cryptococcus neoformans</i> isolated from bird excreta. Thirteen isolates were cultured, colony stained, and underwent biochemical testing confirmation by nested polymerase chain reaction using ITS1-ITS4 and CN4-CN5 primers, respectively. Antifungal susceptibility testing and whole-genomic sequencing were analyzed. The results determined that all isolates were susceptible to amphotericin B (100%), fluconazole, and itraconazole (92.3%). One isolate (DOP3) showed resistance to fluconazole and itraconazole (MIC >64 and >8 µg/mL, respectively). A phylogenetic tree showed the identity of <i>C. neoformans</i> (serotype A). The genome of resistant (DOP3) and non-resistant isolates (DOP3.1) had 14 chromosomes. DOP3 consisted of 38 candidate antifungal resistance genes, which were the most active against azoles (14). The annotated genes in the azole group mostly were in the ATP-binding cassette transporter transmembrane superfamily. Resistance genes against FCZ were in the transcription factors (<i>HAP2, HAP5</i>), zinc finger (<i>NRG1</i>), cytochrome P450 (<i>ERG11</i>), and Myb-like DNA-binding domain (<i>REB1</i>). The most frequent resistance genes against ITZ were cytochrome P450 (<i>ERG5</i> and <i>ERG11</i>) and a transcription factor (<i>HAP5).</i> DOP3.1 also consisted of 26 candidate resistance genes against azoles. Resistance genes against the azole group belong to the <i>ABC</i> transporter transmembrane superfamily. Resistance genes against FCZ belong to cytochrome P450 (<i>ERG11</i>), the zinc finger (<i>NRG1</i>), and the CCAAT binding transcription factor (<i>HAP2</i>). Resistance genes belonging to cytochrome P450 (<i>ERG5</i>) were found against ITZ. This research provides the first report of <i>C. neoformans</i> (serotype A) in zebra dove excreta, drug susceptibility to a resistant strain, and identification of resistance genes. Farm sanitation should be strictly applied, and immunocompetent people should avoid contact with zebra dove excreta.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Addeo et al. Dietary Cannabidiol Supplementation on Growth Performance, Behavior, Blood Profile, Metabolomic Analysis, and Fatty Acid Composition in Rabbits: A Multi-Disciplinary Approach to Improve Welfare and Productivity. <i>Vet. Sci.</i> 2025, <i>12</i>, 759.","authors":"Nicola Francesco Addeo, Valeria Iervolino, Ruggero Amato, Mariarosaria Lanzieri, Daria Lotito, Maria Vittoria Tignani, Alessia Staropoli, Sara Damiano, Pietro Lombardi, Francesco Vinale, Giuliana Parisi, Fulvia Bovera, Nadia Musco, Vincenzo Mastellone","doi":"10.3390/vetsci12090828","DOIUrl":"10.3390/vetsci12090828","url":null,"abstract":"<p><p>In the originally published version of this article [...].</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological Findings of Nestling European Goldfinches (<i>Carduelis carduelis</i>) Co-Infected with <i>Klebsiella pneumoniae</i> and <i>Pseudomonas aeruginosa</i>.","authors":"Jessica Maria Abbate, Giulia D'Annunzio, Rosa Falleti, Claudio Gervasi, Valentina Ravaioli, Elisabetta Lilliu, Emma Santo, Elena Carra, Giovanni Tosi, Giovanni Lanteri","doi":"10.3390/vetsci12090821","DOIUrl":"10.3390/vetsci12090821","url":null,"abstract":"<p><p>Bacterial diseases are widespread in pet birds, posing a severe threat in nestlings and birds with weakened immune systems, often resulting in high mortality during the first days after hatching. This study aimed to describe the pathological findings of a natural bacterial co-infection with <i>Klebsiella pneumoniae</i> and <i>Pseudomonas aeruginosa</i> in nestling European goldfinches (<i>Carduelis carduelis</i>), as a cause of mortality after hatching. Eight nestlings kept in a breeding facility showed an inability to move, anorexia, and respiratory distress, and were found dead between 1 and 4 days of life. Gross pathological findings included diffuse pneumonia with edema and multifocal hemorrhages and occasionally hepatic necrosis. On histopathology, the main findings in all examined birds included severe, subacute bronchopneumonia and severe necrotizing hepatitis. Gram-negative bacilli were observed in parabronchial walls, within pulmonary arteries and surrounding hepatocellular necrotic foci. Lungs, livers and kidneys were sampled for bacteriological examination, resulting in two Gram-negative bacterial isolates. Four housekeeping genes (i.e., 16S rRNA; <i>rpoB</i>; <i>khe</i>; <i>ecfX</i>) were amplified and sequenced for bacterial identification at species level. Although <i>K. pneumoniae</i> and <i>P. aeruginosa</i> are common Gram-negative pathogens and are often co-isolated in human bacterial pneumonia, co-infection with these bacteria has not been documented in nestling goldfinches to date. Pathogen identification is essential for formulating a correct etiological diagnosis and further selecting the most appropriate therapeutic strategy.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving Cattle Health and Welfare in the Area Affected by the First Outbreak of Lumpy Skin Disease in Indonesia.","authors":"Widi Nugroho, Hani Muhamad Mardani, Ando Fahda Aulia, Achmad Efendi, Michael Philipp Reichel","doi":"10.3390/vetsci12090823","DOIUrl":"10.3390/vetsci12090823","url":null,"abstract":"<p><p>This study aimed to investigate cattle farmer livelihoods that relate to cattle welfare in the region with the newly emerging Lumpy Skin Disease (LSD) in Indonesia. A semi-structured interview survey was conducted with randomly selected cattle farmers (n = 102), in Riau. Cattle were bled for analysis of LSD-post-vaccinal seroconversion. The Sustainable Livelihood Framework (SLF) was used; data on livelihood assets, activities, and outcomes were analysed using Multiple Correspondence Analysis (MCA), two-step clustering, and the radar chart of asset possessions. The survey showed that vaccination and veterinary services covered 82.4% and 90.2% of farms. Seroconversion was detectable in vaccinated (15.0%, n = 173) and in non-vaccinated animals (23.1%, n = 13). Farmers mostly fed only grass to cattle (92.2%), with neither pastoral management nor ad libitum water provision. The MCA and cluster analyses indicated that cattle shelter roofing and flooring and manure disposal were the most important markers of the community's livelihood. Poverty among cattle farmers was 23.5%. The cluster with lower income per capita had lower quality of shelter roofing and flooring, a lack of regular manure disposal, jobless second children, and the lowest possession of natural and physical assets. Helping to possess natural and physical assets might improve cattle farmers' well-being and cattle welfare.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA Sequencing-Based Transcriptome Analysis of Liver in Laying Hens Supplemented with Dietary Probiotic <i>Bacillus</i> Species and Prebiotic Yeast (<i>Saccharomyces cerevisiae</i>) Cell Walls.","authors":"Ala E Abudabos, Zafar M Hakami, Ali R Al Sulaiman, Riyadh S Aljumaah, Valentino Palombo, Mashael R Aljumaah, Mariasilvia D'Andrea, Abdulrahman S Alharthi, Rashed A Alhotan","doi":"10.3390/vetsci12090822","DOIUrl":"10.3390/vetsci12090822","url":null,"abstract":"<p><p>To investigate the impacts of dietary <i>Bacillus</i>-based probiotics and yeast-derived prebiotics on the hepatic transcriptome profile, 500 Hisex White laying hens were randomly allotted into five dietary treatments from 37 to 52 weeks of age: control; control + <i>Bacillus subtilis</i>; control + <i>Bacillus subtilis</i> and <i>Bacillus licheniformis</i>; control + <i>Bacillus coagulans</i>; and control + <i>Saccharomyces cerevisiae</i> yeast cell wall. Transcriptome analysis revealed a substantial number of differentially expressed genes exclusively between the control and prebiotic groups, identifying 2221 genes (FDR ≤ 0.05), with 980 genes upregulated (log₂ fold change 0.69 to 24.62) and 1241 downregulated (log₂ fold change -0.74 to -26.46). The top 10 upregulated KEGG pathways included protein export, glycerophospholipid metabolism, tryptophan metabolism, amino acid biosynthesis, alanine, aspartate, and glutamate metabolism, cofactor biosynthesis, propanoate metabolism, ABC transporters, 2-oxocarboxylic acid metabolism, and protein processing within the endoplasmic reticulum. In contrast, the most prominently downregulated pathways encompassed fructose and mannose metabolism, hedgehog signaling, PPAR signaling, Notch signaling, GnRH signaling, cell adhesion molecules, cytokine-cytokine receptor interactions, apelin signaling, glycosaminoglycan degradation, and RIG-I-like receptor signaling. These findings advance understanding of the hepatic transcriptomic response to yeast-derived prebiotics and identify key molecular pathways that could be targeted to enhance metabolic function in laying hens.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection and Prevalence of Equine Piroplasmosis and Other Blood Parasites in Equids of Western Aegean Türkiye.","authors":"Selin Hacilarlioglu, Huseyin Bilgin Bilgic, Tulin Karagenc, Heycan Berk Aydin, Hasan Toker, Hakan Kanlioglu, Metin Pekagirbas, Serkan Bakirci","doi":"10.3390/vetsci12090826","DOIUrl":"10.3390/vetsci12090826","url":null,"abstract":"<p><p>Equine piroplasmosis (EP), caused by <i>Theileria equi</i> and <i>Babesia caballi</i>, is a tick-borne disease posing significant threats to equine health and the horse industry worldwide. Other vector-borne blood parasites, including <i>Anaplasma phagocytophilum</i>, <i>Trypanosoma</i> spp., and <i>Leishmania</i> spp., can also infect horses; however, their prevalence remains poorly characterized in Türkiye. This study aimed to determine the molecular prevalence of <i>T. equi</i>, <i>B. caballi</i>, <i>A. phagocytophilum</i>, <i>Trypanosoma</i> spp., and <i>Leishmania</i> spp. in equids from the Western Aegean Region of Türkiye. Blood samples were collected from 388 clinically healthy equines across İzmir, Aydın, Denizli, and Muğla provinces. Species-specific PCR assays were performed, and <i>T. equi</i> and <i>B. caballi</i> were detected in 24.74% (96/388) and 12.89% (50/388) of samples, respectively, with co-infections in 3.09%. <i>T. equi</i> and <i>B. caballi</i> infections were detected in horses from all four sampled provinces-Aydın, İzmir, Denizli, and Muğla-except for <i>B. caballi</i>, which was not found in any samples from Muğla. No samples tested positive for <i>A. phagocytophilum</i>, <i>Trypanosoma</i> spp., or <i>Leishmania</i> spp. Prevalence significantly varied by province, breed, age, and sex (<i>p</i> < 0.05). This study demonstrates the considerable prevalence of <i>T. equi</i> and <i>B. caballi</i> in Western Türkiye, underlining the need for routine screening and vector control programs. The absence of other parasites suggests limited circulation; however, continued surveillance remains crucial to safeguard equine health and prevent disease spread.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Fennel Essential Oil Supplementation on Mitigating the Heat Stress Impacts on Growth Rate, Blood Biochemical Parameters, and Liver Histopathology in Broiler Chickens.","authors":"Shimaa A Amer, Ahmed Gouda, Rehab I Hamed, Arwa H Nassar, Hanaa S Ali, Rania M Ibrahim, Gehan N Alagmy, Azza M M Abdelmoteleb, Fayez Althobaiti, Khalid S Alotaibi, Shatha B Albattal, Mohamed Mohamed Soliman, Saed A Althobaiti, Gehan K Saleh","doi":"10.3390/vetsci12090825","DOIUrl":"10.3390/vetsci12090825","url":null,"abstract":"<p><p>The current study evaluated the role of dietary fennel essential oil (FO) supplementation in ameliorating the effects of heat stress on growth performance, meat quality, antioxidant activity, inflammatory responses, and liver histopathology in broiler chickens. Six hundred male broiler chicks (three-day-old ROSS broilers) were allocated into six experimental treatments (TRTs); the first was the negative control (Neg. CON, not subjected to hot temperature conditions), and the second was the positive control group (PS CON, subjected to hot temperatures). The control groups (Neg. and PS) were fed the basal diet without supplements. The third, fourth, and fifth groups were fed diets supplemented with 1 g FO/kg diet, 2 g FO/kg diet, and 3 g FO/kg diet, respectively and subjected to hot temperatures. The sixth group was fed the basal diet, received 500 mg of paracetamol per liter of drinking water, and subjected to hot temperatures. Hot temperature conditions (36 ± 0.5 °C) was applied for 6 h/day from the 22nd to the 25th day of the feeding period. The feeding period lasted for 35 days. The results showed that FO supplementation improved the body weight, weight gain, and feed conversion ratio compared to those in the PS CON and paracetamol groups. The growth hormone concentrations increased in the FO-supplemented TRTs and the paracetamol groups compared to those in the Neg. and PS CON groups. The serum total protein, albumin, and globulin concentrations significantly increased in the FO-supplemented TRTs compared to those in the Neg. and PS CON groups and the paracetamol TRTs. The serum TAC increased in the 3 g FO/kg TRT. The serum activity of CAT and SOD increased in the 3 and 2 g FO/kg TRTs and the paracetamol TRTs compared to those in the Neg. and PS CON groups. The serum MDA concentrations decreased in the FO-supplemented TRTs and paracetamol groups compared to those in the Neg. and PS CON groups. The IL1β and IFN-α concentrations decreased in the FO-supplemented and paracetamol groups compared to those in the PS CON groups. The HSP70 concentration was the highest in the 3 g FO/kg TRT. The immune expression of IL1-β and TGF-β in the liver tissues was downregulated in the FO-supplemented groups, especially the FO3 group, compared to those in the PS CON group. In conclusion, dietary supplementation with FO increased the broiler chickens' growth more than that in the PS CON and paracetamol groups under hot temperatures. Fennel oil supplementation (3 g/kg diet) can alleviate the negative impacts of heat stress on broiler chickens' antioxidant and inflammatory responses.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition Heterogeneity and Low-Molecular-Weight Allergen Content of <i>Dermatophagoides farinae</i> House Dust Mite Allergen Extracts Used in Veterinary Medicine.","authors":"Marie Welters, Ana Mas-Fontao, Silvia T Auxilia, Thierry Olivry","doi":"10.3390/vetsci12090824","DOIUrl":"10.3390/vetsci12090824","url":null,"abstract":"<p><p>Extracts used for allergen sensitization testing in humans are heterogeneous in composition and may contain low levels of important allergens. In contrast, there is scant information on the variability of veterinary allergen extracts. We selected two batches of extracts of <i>Dermatophagoides farinae</i> house dust mites from each of three different manufacturers. We observed a 3.4-fold difference in total protein content between the two extracts with the lowest and highest amounts of allergens. After electrophoresis, the band migration patterns were found to be similar between the two batches of extracts from two manufacturers and dissimilar in the third; the migration profiles were heterogeneous between manufacturers. The six extracts did not contain the same concentrations of Der f 1 and Der f 2, with fold changes between the highest and lowest concentrations measured to be 14.3× for Der f 1 and 8.0× for Der f 2. This allergen composition heterogeneity resulted in differences in ELISA seropositivity when allergic dogs had low serum concentrations of IgE against Der f 1 and Der f 2. Our findings demonstrate that, like in human allergology, allergen extracts for veterinary use are also heterogeneous in their protein content and allergen composition. This heterogeneity can impact results when determining allergen sensitizations using crude extracts.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}