Veterinary Sciences最新文献

{"title":"Establishment of a Real-Time Reverse Transcription Recombinase-Aided Isothermal Amplification (qRT-RAA) Assay for the Rapid Detection of Bovine Respiratory Syncytial Virus.","authors":"Guanxin Hou, Siping Zhu, Hong Li, Chihuan Li, Xiaochen Liu, Chao Ren, Xintong Zhu, Qiumei Shi, Zhiqiang Zhang","doi":"10.3390/vetsci11120589","DOIUrl":"10.3390/vetsci11120589","url":null,"abstract":"<p><strong>Background: </strong>Bovine respiratory syncytial virus (BRSV) is a significant cause of bovine respiratory disease, resulting in significant losses to the cattle industry. For rapid detection of BRSV, a real-time recombinase-aided isothermal amplification assay (qRT-RAA) based on the <i>F</i> gene of BRSV was developed in this study.</p><p><strong>Results: </strong>The developed qRT-RAA assay showed good exponential amplification of the target fragment in 20 min at a constant temperature of 39 °C. And this assay displayed a high specificity for BRSV, without cross-reactions with Infectious Bovine Rhinotracheitis Virus (IBRV), Bovine Parainfluenza Virus Type 3 (BPIV3), Bovine Viral Diarrhea Virus (BVDV), and Bovine Coronavirus (BCoV). With the standard RNA of BRSV serving as a template, the limit of detection for qRT-RAA was 102 copies/μL. We examined ninety-seven clinical samples from cattle with respiratory disease using this method and determined a positive rate of 7.2% (7/97), consistent with results using the classical PCR method reported previously.</p><p><strong>Conclusions: </strong>A qRT-RAA assay for BRSV detection was established in this study. The method is specific and sensitive and can be completed within 20 min at 39 °C. These works demonstrate that the generated qRT-RAA assay is an effective diagnostic tool for rapidly detecting BRSV in resource-limited settings, which may be applied for the clinical detection of BRSV.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Indigo Waste Silage Prepared with Additives on Feed Availability, Rumen Fermentation Patterns, Blood Metabolites, and Hematological Indices in Beef Cattle.","authors":"Nirawan Gunun, Chatchai Kaewpila, Waroon Khota, Wasana Phlaetita, Pongsatorn Gunun","doi":"10.3390/vetsci11120588","DOIUrl":"10.3390/vetsci11120588","url":null,"abstract":"<p><p>The purpose of this study was to evaluate the impact of different additives in fermented indigo waste on feed availability, rumen fermentation patterns, blood chemistry, and hematology in beef cattle. Four male crossbred beef cattle with a body weight (BW) of 230 ± 14 kg and 25 months of age were used in a 4 × 4 Latin square design. The indigo waste was ensiled without additive (CON) and with calcium hydroxide (CH), molasses (M), or cellulase (C). The indigo waste silage was incorporated into a fermented total mixed ration (FTMR). The neutral detergent fiber (NDF) content was lower in C-treated silage. The feed intake did not alter among treatments (<i>p</i> > 0.05). The ensiled indigo waste with additives had no influence on nutrient digestibility (<i>p</i> > 0.05), whereas the ensiled indigo waste with M increased CP digestibility (<i>p</i> < 0.01). The rumen pH, ammonia-nitrogen (NH₃-N), or volatile fatty acid (VFA) proportions did not change by additive (<i>p</i> > 0.05). However, adding M increased the acetate-to-propionate ratio (C2:C3) (<i>p</i> = 0.04). In addition, the total protein in the blood was higher in C-treated silage (<i>p</i> = 0.01). The homological indices did not change by additive (<i>p</i> > 0.05), except for lymphocytes, which decreased when M and C were added (<i>p</i> < 0.01). In conclusion, adding M and C to indigo waste silage could enhance its nutritional value and improve digestibility, blood chemistry, or health status in beef cattle.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress Reduction in Alpaca (<i>Vicugna pacos</i>) Mange Management.","authors":"Marilena Bolcato, Mariana Roccaro, Filippo Maria Dini, Arcangelo Gentile, Angelo Peli","doi":"10.3390/vetsci11110587","DOIUrl":"10.3390/vetsci11110587","url":null,"abstract":"<p><p>Stress and dermatosis or itching are strictly related to mange caused by <i>Sarcoptes</i> spp. and <i>Chorioptes</i> spp. mites, which are particularly stressful in alpacas. Treatment is challenging due to limited options, poor response, and low topical efficacy. Paradoxically, veterinary procedures can exacerbate stress, worsening the clinical conditions of these fragile prey species. This case report aims to highlight the key role of stress in the onset and evolution of dermatological diseases. Three alpacas, introduced to an animal theme park, developed dermatological issues three months later. Clinical exams revealed mild itching, and alopecic and hyperkeratotic lesions on their ears, belly, and limbs. Skin scrapings confirmed <i>Sarcoptes scabiei</i> and <i>Chorioptes bovis</i>. Subcutaneous ivermectin was administered at 0.2 mg/kg, repeated at 7, 14, and 21 days. Weekly inspections monitored disease progression and stress responses, including increased vigilance, running, jumping, vocalizations, tachycardia, and tachypnoea. Due to the persistent stress and despite positive parasitological test results, treatment was suspended after day 21, with remote disease monitoring. A month later, the parasitological exam was negative, and three months later, all alpacas showed no clinical signs of complete hair regrowth. Understanding and addressing stress in alpacas is vital in veterinary practice for effective disease management and overall well-being.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Mutated <i>BRAF</i>^V595E Kinase in Canine Carcinomas-An Immunohistochemical Study.","authors":"Annika Bartel, Heike Aupperle-Lellbach, Alexandra Kehl, Silvia Weidle, Leonore Aeschlimann, Robert Klopfleisch, Simone de Brot","doi":"10.3390/vetsci11110584","DOIUrl":"10.3390/vetsci11110584","url":null,"abstract":"<p><p>Alterations of the <i>BRAF</i> gene and the resulting changes in the BRAF protein are one example of molecular cancer profiling in humans and dogs. We tested 227 samples of canine carcinomas from different anatomical sites (anal sac (<i>n</i> = 23), intestine (<i>n</i> = 21), liver (<i>n</i> = 21), lungs (<i>n</i> = 19), mammary gland (<i>n</i> = 20), nasal cavity (<i>n</i> = 21), oral epithelium (<i>n</i> = 18), ovary (<i>n</i> = 20), prostate (<i>n</i> = 21), thyroid gland (<i>n</i> = 21), urinary bladder (<i>n</i> = 22)) with two commercially available primary anti-BRAF^V600E antibodies (VE1 Ventana, VE1 Abcam). The immunohistochemical results were confirmed with droplet digital PCR (ddPCR). <i>BRAF^V595E</i>-mutated cases were found in canine prostatic (16/21), urothelial (17/22), and oral squamous cell carcinomas (4/18), while other carcinoma types tested negative. Both antibodies showed consistent results, with intracytoplasmic immunolabeling of tumour cells, making them reliable tools for detecting the <i>BRAF^V595E</i> mutation in canine carcinomas. In conclusion, identifying <i>BRAF</i> mutations from biopsy material offers a valuable opportunity to enhance cancer treatment strategies (BRAF inhibitors) in canine urothelial carcinomas, prostatic carcinomas, and oral squamous cell carcinomas.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helminthofauna Diversity in Synanthropic Rodents of the Emilia-Romagna Region (Italy): Implications for Public Health and Rodent Control.","authors":"Filippo Maria Dini, Carlotta Mazzoni Tondi, Roberta Galuppi","doi":"10.3390/vetsci11110585","DOIUrl":"10.3390/vetsci11110585","url":null,"abstract":"<p><p>Synanthropic rodents are species well adapted to coexisting in anthropogenically influenced environments. This coexistence raises concerns about the potential risks of pathogen's transmission due to their close proximity to human habitats. This study presents an epidemiological survey of the gastrointestinal helminth fauna in synanthropic rodents (<i>Mus musculus</i>, <i>Rattus rattus</i>, and <i>Rattus norvegicus</i>) from the Emilia-Romagna Region (Italy), aiming to provide updated data on the endoparasitic populations in these species. A total of 111 rodents, sampled from 2019 to 2021 during pest control programs, were examined for parasitic infections. Helminths were extracted through necropsy and microscopic analysis of gastrointestinal tracts and sediment, with species identification based on morphological characteristics. Overall, 72.1% of the rodents were found to be parasitized, with nematodes being the most prevalent. <i>Syphacia muris</i>, <i>Aspiculuris tetraptera</i>, <i>Nippostrongylus brasiliensis</i>, and <i>Heterakis spumosa</i> were the most frequently identified nematodes. Tapeworms, including <i>Rodentolepis nana</i> and <i>Hymenolepis diminuta</i>, were also detected, albeit in lower frequencies. The trematode <i>Brachylaima recurva</i> was recovered only in one <i>R. rattus</i>. Co-infection was common, particularly among rats, with 51.8% of black rats and 22% of brown rats harboring multiple parasitic species. Mice exhibited lower levels of polyparasitism, with only two individuals showing mixed infections. Interestingly, disparities between the detection of adult helminths and parasitic eggs were noted, especially in cases where no adults were observed, but eggs were found through sediment analysis. These findings suggest that traditional necropsy, especially with poorly preserved carcasses, may underestimate parasite prevalence. This highlights the importance of combining necropsy with microscopic techniques, such as flotation and sedimentation, for a more thorough assessment. Using these methods, nematodes with direct life cycles, such as <i>Syphacia spp.</i>, <i>Nippostrongylus brasiliensis</i>, and <i>Heterakis spumosa</i>, have been confirmed as widespread and cosmopolitan among rodent populations. The detection of zoonotic parasites raises concerns about potential transmission to humans, particularly in areas with poor sanitation and high rodent densities. These findings underscore the need for integrated rodent control and environmental sanitation to reduce zoonotic risks.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple Endocrine Neoplasia with Multiple PGLs in Two Boxer Dogs: Morphological Features, Immunohistochemical Profile and SDHD Gene Mutation Screening.","authors":"Ecaterina Semzenisi, Roxana Popa, Corina Toma, Valentin-Adrian Bâlteanu, Iuliu Calin Scurtu, Romelia Pop, Alexandru-Flaviu Tăbăran","doi":"10.3390/vetsci11110586","DOIUrl":"10.3390/vetsci11110586","url":null,"abstract":"<p><p>This study aimed to describe the gross and histological features of multiple endocrine and non-endocrine neoplasia, including multiple PGLs found in two Boxer dogs. Additionally, the identified PGLs were immunohistochemically evaluated, and the subunits 2, 3, and 4 of the SDHD gene were screened for possible mutations. The tumors identified include aortic and carotid body PGLs, thyroid follicular-compact carcinoma, and subcutaneous lipomas. One case also had a Leydig cell tumor and adrenal cortex hyperplasia, while the other had H-type pancreatic carcinoma. Three out of 4 PGLs appeared benign, but one aortic body tumor showed malignant features with neoplastic emboli at its edge. Immunohistochemical analysis confirmed the neuroendocrine origin of all PGLs, with positive staining for Chromogranin A, NSE, and variable positivity for S100. No somatic mutations were found in exons 2, 3, and 4 of the SDHD gene in any of the evaluated PGLs. The absence of mutations in the evaluated SDHD gene subunits suggests the involvement of other genetic factors or pathways in the development of these tumors, warranting further investigation in this field.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outbreak of Esophagitis and Ingluvitis Caused by <i>Salmonella</i> Typhimurium in Passeriform Birds of the Genus <i>Sporophila</i> Seized from Wildlife Trafficking.","authors":"Karoline L Soares, Ricardo B Lucena, Ewerton S Lima, Millena de O Firmino, Lilian R C Eloy, Raquel Annes F Silva, Mônica S Sousa, Isabelle V Sousa, Weslley Drayton Q Silva, Artur Cezar de C Fernandes, Eduardo M Ramos-Sanchez","doi":"10.3390/vetsci11110582","DOIUrl":"10.3390/vetsci11110582","url":null,"abstract":"<p><p>The occurrence of esophagitis and ingluvitis caused by <i>Salmonella</i> Typhimurium in passerines seized from illegal wildlife trafficking is described. This illegal activity causes stress and leads to lowered immunity in the birds. Additionally, inadequate hygiene conditions predispose the birds to diseases such as salmonellosis. Few studies report the occurrence of <i>Salmonella</i>-induced lesions in the esophagus and crop of passerines; therefore, this study aimed to describe the disease in birds of the genus <i>Sporophila</i>, as well as to investigate the presence of the bacterium in the environment and determine the antimicrobial resistance profile of the isolated bacteria. Three birds of the genus <i>Sporophila</i> were necropsied. In the esophagus and crop, yellowish plaques corresponding to necrosis with bacterial aggregates were observed. <i>Salmonella</i> spp. was isolated from these lesions, with genus confirmation via MALDI-TOF mass spectrometry. Environmental samples were collected from the enclosures and cages where the animals were quarantined, and the same bacterium was isolated. In two birds, the serotype S. Typhimurium was identified. Antibiograms performed on the strains from the birds and the environment revealed resistance to antibiotics in the classes of penicillins, sulfonamides, aminoglycosides, monobactams, tetracyclines, and first and third-generation cephalosporins. To the authors' knowledge, this is the first report of this agent causing death in <i>Sporophila</i> due to esophagitis and ingluvitis. It is also the first report of salmonellosis in three species of passerines in Brazil. The study underscores the importance of understanding the pathogens circulating in wild animals, especially within the context of One Health.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Cryopreservation of Bovine Ovarian Tissue by Analysis of Reactive Species of Oxygen, Toxicity, Morphometry, and Morphology.","authors":"Camila Bizarro-Silva, Larissa Zamparone Bergamo, Camila Bortoliero Costa, Suellen Miguez González, Deborah Nakayama Yokomizo, Ana Carolina Rossaneis, Waldiceu Aparecido Verri Junior, Mateus José Sudano, Evelyn Rabelo Andrade, Amauri Alcindo Alfieri, Marcelo Marcondes Seneda","doi":"10.3390/vetsci11110579","DOIUrl":"10.3390/vetsci11110579","url":null,"abstract":"<p><p>Ovarian tissue cryopreservation has been widely investigated for preserving female fertility. In the present study, we aimed to compare the effects of three concentrations (1, 1.5, and 3 M) of dimethylsulfoxide (DMSO) on the vitrification of ovarian tissue. The ovarian cortex was divided into control and vitrified groups: (i) 1 M-DMSO, (ii) 1.5 M-DMSO, and (iii) 3 M-DMSO. Follicles from all fragments were analyzed for DMSO-induced deleterious effects, morphological and morphometric aspects, and concentration of reactive oxygen species. Additionally, the fragments were cultured to assess the integrity and return of follicular development post-vitrification. All DMSO concentrations resulted in a higher percentage of degenerated preantral follicles than before the cryopreservation process. After vitrification, the cryopreserved ovarian fragments showed similar percentages of intact follicles; however, the 3 M DMSO concentration differed from the control. Analyzing free radical production, we found that the 3 M DMSO concentration had higher levels of oxidative stress than the lower DMSO. After in vitro cultivation of the vitrified/warmed fragments, the 1 M DMSO concentration exhibited higher percentages of morphologically intact follicles than the other concentrations. Therefore, we suggest that bovine preantral follicles can be cryopreserved in situ with greater efficiency in 1 M DMSO.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Haematological Ratios at: Different Stages of Canine Periodontal Disease.","authors":"Carolina Silva, Ana Carolina Abrantes, Ana Carolina Fontes, Isabel Dias, Rosário Domingues, Francisco Peixoto, Carlos Viegas","doi":"10.3390/vetsci11110581","DOIUrl":"10.3390/vetsci11110581","url":null,"abstract":"<p><p>This is a retrospective study about haematological ratios in different stages of canine periodontal disease in one single breed, more specifically the Portuguese Podengo. The aim of the study was to assess the clinical significance of five haematological ratios, namely neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), mean platelet volume-to-platelet count ratio (MPV/PLT), monocyte-to-lymphocyte ratio (MLR), and platelet-to-neutrophil ratio (PNR), easily obtained through the parameters provided by the complete blood count, in the stages of gingivitis and periodontitis, compared to clinical healthy individuals. Receiver operating characteristic curves were generated to identify ideal sensitivity and specificity cut-offs for cases of gingivitis or periodontitis. Our study included 80 dogs, which comprised 24 healthy dogs, 26 diagnosed with gingivitis, and 30 with periodontitis. The median NLR was significantly lower in periodontitis than in clinical healthy dogs (<i>p</i> = 0.040) and in dogs with gingivitis (<i>p</i> = 0.037). The median PLR was significantly lower in cases of gingivitis (<i>p</i> = 0.020) and periodontitis (<i>p</i> = 0.024) than in healthy dogs. MPV/PLT and MLR did not demonstrate significant differences between any of the three groups. The median PNR was significantly lower in gingivitis than in control dogs (<i>p</i> = 0.019). PNR had the highest accurate results, in distinguishing between healthy individuals and those with gingivitis, with an area under the curve of 0.692 (95% CI [0.539-0.845], <i>p</i> = 0.020). However, even this index had weak discriminatory power between the groups in the analysis. The impact of systemic inflammation generated by canine periodontal disease can thus be seen through some of these indices. However, the results obtained here demonstrate the need for complementarity with other methods to diagnose canine periodontal disease and reinforce the need for additional studies with more markers of systemic inflammatory response.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Biomarkers of Inflammation and Oxidative Status in Dogs Subjected to Celiotomy or Video-Assisted Ovariohysterectomy.","authors":"Fabíola Dalmolin, Camila Peres Rubio, Carla Sordi Furlanetto, Rafael Steffens, Najla Ibrahim Isa Abdel Hadi, Adriellen de Lima da Silva, Paloma Tomazi, Bernardo Nascimento Antunes, Fabiana Elias, Elizabeth Moreira Dos Santos Schmidt, Maurício Veloso Brun","doi":"10.3390/vetsci11110583","DOIUrl":"10.3390/vetsci11110583","url":null,"abstract":"<p><p>We evaluated the surgical stress response of dogs undergoing three ovariohysterectomy (OVH) techniques. Twenty-nine healthy females were allocated into groups: celiotomy and ligature (CelioSut), celiotomy and bipolar energy (CelioBip), and a video-assisted technique using two portals and bipolar energy (VidBip). Clinical evaluation was performed, and the following blood analyses were determined: acute phase proteins (C-reactive protein and haptoglobin), white blood cell counting (WBC), and biomarkers of oxidative status. The VidBip required more time despite a smaller incision, lower heart rate, and earlier feeding and urination. All groups had high white blood cells counts; the C-reactive protein (CRP) levels peaked at 6 and 12 h in all groups and was higher in the CelioBip and VidBip groups compared to CelioSut; haptoglobin concentrations peaked at 48 h in all groups and increased in dogs of the CelioSut group. Trolox equivalent antioxidant capacity, ferric reducing ability of plasma, cupric reducing antioxidant capacity, and advanced oxidation protein were not significantly different among the groups or time points. Total thiol concentrations were lower in CelioBip and CelioSut groups. All surgical techniques induced an inflammatory and oxidative stress response, but the video-assisted technique produced early clinical recovery. The bipolar device produces fewer disturbances than suspensory ligament rupture and ligature.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}