Veterinary Sciences最新文献_第10页

Cultured Bacteria in the Abdominal Wall Incision During the Realisation of Caesarean Section in Cows: A Preliminary Study.

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-30 DOI: 10.3390/vetsci12020098

Salem Djebala, Elise Coria, Florian Munaut, Linde Gille, Justine Eppe, Nassim Moula

{"title":"Cultured Bacteria in the Abdominal Wall Incision During the Realisation of Caesarean Section in Cows: A Preliminary Study.","authors":"Salem Djebala, Elise Coria, Florian Munaut, Linde Gille, Justine Eppe, Nassim Moula","doi":"10.3390/vetsci12020098","DOIUrl":"https://doi.org/10.3390/vetsci12020098","url":null,"abstract":"Although caesarean section (CS) is a common surgery, the bacteria contaminating the abdominal incision during this operation are not documented. This study aimed to enhance perioperative antibiotic therapy and reduce post-operative surgical site infections by describing the bacteria contaminating the abdominal incision during CS. Bacterial culture was performed on swabs sampled from the abdominal incision (muscular layers) of 23 cows during the realisation of CS. Bacteriology was positive in 6/22 samples. while 16/22 were negative (p = 0.03), and one sample was contaminated. All identified bacteria were aerobic, and 8/14 isolates were Gram-positive and 6/14 were Gram-negative (p = 0.7). The most encountered species were Acinetobacter spp., Aerococcus viridans, Neisseria spp. and Streptococcus spp.; other species were isolated only once. The abdominal incision of elective CS can be contaminated by ubiquitous aerobic Gram-positive and Gram-negative species coming from the environment. This contamination might be avoided by using a better aseptic protocol and by improving the conditions in which CSs are performed. However, more samples are required, and antibiotic susceptibility determination is needed to identify the adequate pre-operative antibiotic.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020-2023.

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-30 DOI: 10.3390/vetsci12020099

Hongyu Chen, Yi Qing, Lei Xu, Ling Zhu, Wenqi Yin, Shuwei Li, Shengyao Kuang, Yuancheng Zhou, Zhiwen Xu

{"title":"Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020-2023.","authors":"Hongyu Chen, Yi Qing, Lei Xu, Ling Zhu, Wenqi Yin, Shuwei Li, Shengyao Kuang, Yuancheng Zhou, Zhiwen Xu","doi":"10.3390/vetsci12020099","DOIUrl":"https://doi.org/10.3390/vetsci12020099","url":null,"abstract":"Porcine parvovirus (PPV) is a non-enveloped, single-stranded linear DNA virus that induces reproductive disorders in sows, particularly abortions in primiparous sows. This study investigated the prevalence of PPV in the southwestern region and conducted molecular characterization of PPV strains. An epidemiological survey was conducted on 1534 aborted fetuses from the southwestern region between 2020 and 2023, revealing an abortion rate of 3.00% due to PPV2, with the highest rate of 3.77% in Sichuan. Additionally, 2973 blood samples from sows were tested using ELISA, showing a PPV2 antibody positivity rate of 73.03% to 90%. Through shotgun metagenomics, PPV2 SC2020 was identified in aborted fetal samples from a pig farm in Pengzhou, Sichuan. PCR sequencing analysis yielded seven PPV2 genomic sequences, and the phylogenetic analysis of eight PPV2 strains with thirty reference strains showed distinct evolutionary branches. The virus was successfully isolated from PPV2-positive samples, and the phylogenetic analysis of PPV2 SC2020 revealed ORF1 gene homology of 94.9% to 99.3% and the ORF2 gene homology of 93.1% to 98.0%, with 34 reference strains. Homologous recombination analysis indicated that SC2020 is a recombinant strain of HeB03 and S1.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luteolin Alleviates Inflammation Induced by Staphylococcus aureus in Bovine Mammary Epithelial Cells by Attenuating NF-κB and MAPK Activation.

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-27 DOI: 10.3390/vetsci12020096

Yingfang Guo, Jinxin Zhang, Ting Yuan, Cheng Yang, Qingqing Zhou, Aftab Shaukat, Ganzhen Deng, Xiaoyan Wang

{"title":"Luteolin Alleviates Inflammation Induced by Staphylococcus aureus in Bovine Mammary Epithelial Cells by Attenuating NF-κB and MAPK Activation.","authors":"Yingfang Guo, Jinxin Zhang, Ting Yuan, Cheng Yang, Qingqing Zhou, Aftab Shaukat, Ganzhen Deng, Xiaoyan Wang","doi":"10.3390/vetsci12020096","DOIUrl":"https://doi.org/10.3390/vetsci12020096","url":null,"abstract":"The internalization of S. aureus in bMECs is a major pathogenic mechanism leading to mastitis, causing significant economic losses in the dairy industry. Numerous plants contain Lut, a natural flavonoid with anti-inflammatory and antioxidant properties. However, little is known about Lut's ability to reduce inflammation caused by S. aureus in bMECs. This research aimed to evaluate the mechanism by which Lut reduces S. aureus-induced inflammation in bMECs. Through GO and KEGG enrichment analysis, researchers analyzed the differentially expressed genes in bMECs infected with S. aureus in NCBI GEO (GSE139612) and also analyzed the targets of Lut predicted by various online platforms. These studies identified two overlapping signaling pathways, the NF-κB and the MAPK pathways. We stimulated bMECs with S. aureus for two hours and then added Lut for ten hours, with a total duration of twelve hours. The expression levels of TLR2-MyD88-TRAF6 components, inflammatory cytokines, and protein phosphorylation associated with the MAPK and NF-κB signaling pathways were then assessed. Based on all of the results, Lut inhibited the generation of inflammatory cytokines in bMECs that were induced by S. aureus through the TLR2, NF-κB, and MAPK signaling pathways. This process might account for the anti-inflammatory properties of Lut.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Maternal Obesity on Placental Autophagy in Lean Breed Sows.

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-27 DOI: 10.3390/vetsci12020097

Liang Tian, Fen Su, Xueyi Zhu, Xingyue Zou

{"title":"The Effect of Maternal Obesity on Placental Autophagy in Lean Breed Sows.","authors":"Liang Tian, Fen Su, Xueyi Zhu, Xingyue Zou","doi":"10.3390/vetsci12020097","DOIUrl":"https://doi.org/10.3390/vetsci12020097","url":null,"abstract":"This study aimed to evaluate the influence of back-fat thickness (BF), at mating of sows, on autophagy in placenta and the potential mechanism. The sows were divided into two groups according to their BF at mating: BFI (15-20 mm, n = 14) and BFII (21-27 mm, n = 14) as the maternal obesity group. The placental samples used for investigating autophagic function and fatty acid profiles were obtained by vaginal delivery. Our results demonstrated that autophagy defects were observed in placenta from BFII sows along with altered circulating and placental fatty acid profiles. Indicative of impaired autophagy, reduced autophagic vesicles as well as LC3-positive puncta were linked to decreased mRNA or protein expression of autophagy-related genes, including ATG5, ATG7, Beclin1, ATG12, LC3, LAMP1 and LAMP2 in the placenta of BFII sows (p < 0.05). Meanwhile, we found reduced conversion of LC3-I to LC3-II and up-regulated protein content of p62 in the placenta from BFII group (p < 0.05). Furthermore, excessive back-fat was also associated with increased activation of AKT/mTOR signaling and decreased mRNA content of transcription factors regulating the autophagic pathway, including PPARα and PGC1α, but increased mRNA expression of NcoR1 in placenta. Together, these findings indicate that maternal obesity incites autophagy injury in pig term placenta, which may contribute to augmented placental lipid accumulation and therefore impaired placental function.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Transcriptome Analysis Reveals the Lung miRNA-mRNA Regulatory Network Associated with Avian Pathogenic E. coli Infection.

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-26 DOI: 10.3390/vetsci12020095

Huan Li, Jishuang Tan, Xiaoyi Li, Susan J Lamont, Hongyan Sun

{"title":"Integrated Transcriptome Analysis Reveals the Lung miRNA-mRNA Regulatory Network Associated with Avian Pathogenic E. coli Infection.","authors":"Huan Li, Jishuang Tan, Xiaoyi Li, Susan J Lamont, Hongyan Sun","doi":"10.3390/vetsci12020095","DOIUrl":"https://doi.org/10.3390/vetsci12020095","url":null,"abstract":"Avian pathogenic E. coli (APEC), one of the most common pathogens, can cause localized or systemic infections and lead to significant economic losses in the poultry industry annually. Recently, evidence suggests that microRNAs (miRNAs) play important roles in the host immune response to bacterial infection by targeting mRNAs. However, few studies have examined the immune mechanisms of miRNAs and mRNAs in chicken lungs following APEC infection. Herein, hematoxylin-eosin staining and qRT-PCR were employed to investigate APEC-induced lung inflammation in chickens. RNAseq was used to identify the miRNAs and mRNAs expression profile between the APEC infection group (APEC) vs. the Control group (Control). The results show that APEC can induce lung lesions in chickens and increase the expression levels of inflammatory cytokines (IL1β, IL8, IL6, and TNFα). High-quality sequencing data were obtained, of which more than 93% of the reads can be mapped to the chicken genome. A total of 22 differentially expressed (DE) miRNAs and 608 DE mRNAs were detected in the APEC vs. the Control. Remarkably, 23 regulatory pairs of miRNA-mRNA interactions were identified in chicken lungs upon APEC infection. Further validation revealed that gga-miR-214 could directly target the RAB37 gene upon APEC infection to modulate the expression of inflammatory cytokine response. This study provides new insights into the host immune response to APEC infection.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histoplasma capsulatum in Bat Species in Portugal.

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-25 DOI: 10.3390/vetsci12020094

Jaqueline T Bento, Ana Cláudia Coelho, Hugo Rebelo, João R Mesquita

{"title":"Histoplasma capsulatum in Bat Species in Portugal.","authors":"Jaqueline T Bento, Ana Cláudia Coelho, Hugo Rebelo, João R Mesquita","doi":"10.3390/vetsci12020094","DOIUrl":"https://doi.org/10.3390/vetsci12020094","url":null,"abstract":"Histoplasmosis, caused by the fungus Histoplasma capsulatum, poses health risks to various mammals, including humans. Bats are primary wild carriers of Histoplasma capsulatum, playing a crucial role in its epidemiology. However, fecal shedding in Europe remains poorly studied, with no data available for Portugal. This study analyzed 285 guano samples from 22 bat species, collected across Portuguese regions between 2014 and 2018, using a nested PCR assay. Despite using a sensitive method, no positive samples were detected. These results align with other European studies, suggesting that Histoplasma capsulatum circulates at low levels in European bat populations. However, they contrast with findings from regions like Brazil and Mexico, where the fungus is more prevalent due to differing geographic, climatic, and ecological factors. The absence of Histoplasma capsulatum in Portuguese bat guano highlights the importance of local environmental conditions and raises questions about its distribution in Europe. Although bats can harbor zoonotic pathogens, our findings suggest they do not shed Histoplasma capsulatum in Portugal. Continuous monitoring and research are essential in understanding infectious disease dynamics. Targeted surveillance in caves could improve early detection and management strategies for potential histoplasmosis outbreaks, contributing to public health efforts in these ecosystems.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and Sensitive One-Tube Detection of Getah Virus Using RT-LAMP Combined with Pyrococcus furiosus Argonaute.

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-24 DOI: 10.3390/vetsci12020093

Zhong Liu, Fosheng Yang, Mengtao Fang, Qi Wu, Ke Fan, Dongyan Huang, Yu Ye, Gen Wan, Deping Song

{"title":"Rapid and Sensitive One-Tube Detection of Getah Virus Using RT-LAMP Combined with Pyrococcus furiosus Argonaute.","authors":"Zhong Liu, Fosheng Yang, Mengtao Fang, Qi Wu, Ke Fan, Dongyan Huang, Yu Ye, Gen Wan, Deping Song","doi":"10.3390/vetsci12020093","DOIUrl":"https://doi.org/10.3390/vetsci12020093","url":null,"abstract":"Getah virus (GETV) is a mosquito-borne virus that poses a significant threat to both animal and public health. Traditional diagnostic methods for GETV, such as RT-PCR and RT-qPCR, require expensive equipment and complex procedures, making them unsuitable for rapid, on-site detection. The combination of RT-LAMP and PfAgo offers a novel approach for nucleic acid detection, providing high specificity and effective without the need for sophisticated instruments. Herein, we developed a RT-LAMP combined with PfAgo assay for GETV detection. The RT-LAMP assay was conducted at 60~65 °C, and then the RT-LAMP product was cleaved, together with a fluorescent probe, mediated by PfAgo at 95 °C. After optimizing the primary reaction conditions, the detection limit of the RT-LAMP-PfAgo assay was 100 copies/µL. Importantly, there was no cross-reactivity with other viruses, including PEDV, PDCoV, PoRV, PRRSV, and CSFV. Compared to qPCR, analysis of 86 clinical samples showed that LAMP-PfAgo had a consistent positive rate with the qPCR method. In conclusion, we developed a valuable diagnostic tool for the rapid detection of GETV, enabling timely surveillance and control measures to mitigate the impact of GETV outbreaks.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk Assessment and Prevention of Foot-and-Mouth Disease Transmission from Laos to China.

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-24 DOI: 10.3390/vetsci12020092

Jige Xin, Sixian Lan, Jun Ai, Bangquan Zeng, Aiguo Xin, Lingling Ye, Weidong Zuo, Yanlin Li, Diangang Han

{"title":"Risk Assessment and Prevention of Foot-and-Mouth Disease Transmission from Laos to China.","authors":"Jige Xin, Sixian Lan, Jun Ai, Bangquan Zeng, Aiguo Xin, Lingling Ye, Weidong Zuo, Yanlin Li, Diangang Han","doi":"10.3390/vetsci12020092","DOIUrl":"https://doi.org/10.3390/vetsci12020092","url":null,"abstract":"Foot-and-mouth disease (FMD) is classified as a Class I animal disease in China and listed as one of the notifiable animal diseases by the World Organization for Animal Health (WOAH). It significantly impacts the safe production of livestock and the trade of animals and related products. China's Yunnan Province shares a 710 km border with Laos, with frequent cross-border trade, and the cross-border flow of animals and related products occurs from time to time. In order to prevent the introduction of FMD from the border areas of Laos into China, this study conducted an assessment of the entry, exposure, and consequences of FMD transmission. The findings revealed a \"high\" risk in terms of entry assessment, a \"medium\" risk in exposure assessment, and a \"high\" risk in the consequence assessment. Based on these assessments, the overall risk level for the introduction of FMD from Laos into China is determined to be \"high\". Therefore, it is recommended that management measures are implemented, such as restricting animal movement across borders and strengthening inspection procedures for animals entering China, to effectively prevent FMD introduction from Laos.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin Barrier in Normal and Allergic Horses: What Do We Know?

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-24 DOI: 10.3390/vetsci12020091

Rosanna Marsella

{"title":"Skin Barrier in Normal and Allergic Horses: What Do We Know?","authors":"Rosanna Marsella","doi":"10.3390/vetsci12020091","DOIUrl":"https://doi.org/10.3390/vetsci12020091","url":null,"abstract":"Information on skin barrier in horses is limited. A study on the epidermal ultrastructure of normal and allergic horses documented disorganized amorphous intercellular lipids in the stratum corneum of allergic samples. These findings are similar to atopic canine and human skin. Currently, there is no published study comparing skin barrier function parameters between normal and allergic horses; thus, the functional implications of the ultrastructural changes are unknown. In normal horses, body location, gender, breed, and ambient conditions affect skin barrier parameters, such as Transepidermal Water Loss. Skin microbiome studies on normal horses have highlighted the importance of season and environmental conditions, since horses housed together share similar microbiomes. Skin dysbiosis and predominance of staphylococcus have been described in horses with pastern dermatitis. Transcriptomic studies of the epidermis of normal and allergic horses have found that lesional allergic skin has substantial transcriptomic differences when compared with healthy skin, namely downregulation of genes of tight junctions, keratins, and upregulation of serine proteases and IL-13. Keratinocytes harvested from horses with insect bite hypersensitivity show upregulation of IL-31 gene expression under stimulation. While more research is clearly needed, preliminary results seem to support skin barrier differences between normal and allergic horses.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin Modulates ZAP70 and CD40 Transcripts via Histone Modifications in Canine Ileum Epithelial Cells.

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-01-23 DOI: 10.3390/vetsci12020087

Jian Hong, Saber Y Adam, Shiqi Wang, Hao Huang, In Ho Kim, Abdelkareem A Ahmed, Hao-Yu Liu, Demin Cai

{"title":"Melatonin Modulates ZAP70 and CD40 Transcripts via Histone Modifications in Canine Ileum Epithelial Cells.","authors":"Jian Hong, Saber Y Adam, Shiqi Wang, Hao Huang, In Ho Kim, Abdelkareem A Ahmed, Hao-Yu Liu, Demin Cai","doi":"10.3390/vetsci12020087","DOIUrl":"https://doi.org/10.3390/vetsci12020087","url":null,"abstract":"Melatonin (MLT), produced by the pineal gland and other tissues, is known for its anti-inflammatory effects, particularly in regulating inflammatory markers and cytokines in intestinal cells. Our study aimed to investigate how MLT influences the expression of inflammatory genes through histone modification in canine ileum epithelial cells (cIECs). In our experiment, cIECs were cultured and divided into a control group (CON) and an MLT-treatment group. MLT did not significantly affect cell growth or death in cIECs compared to the CON. However, MLT treatment led to an upregulation of CD40, ZAP70, and IL7R and a downregulation of LCK, RPL37, TNFRSF13B, CD4, CD40LG, BLNK, and CIITA at the mRNA expression level. Moreover, MLT significantly altered the NF-kappa B signaling pathway by upregulating genes, such as CD40, ZAP70, TICAM1, VCAMI, GADD45B, IRAK1, TRADD, RELA, RIPK1, and RELB, and downregulating PRKCB, LY96, CD40LG, ILIB, BLNK, and TNFRSF11A. Using ChIP-qPCR, we discovered that MLT treatment enhanced histone acetylation marks H3K9ac, H3K18ac, H3K27ac, and methylation marks H3K4me1 and H3K4me3 at the ZAP70 and CD40 gene loci (p < 0.05). Additionally, the enrichment of RNA polymerase II and phosphorylated Ser5 pol-II at these loci was increased in MLT-treated cells (p < 0.05), indicating heightened transcriptional activity. In conclusion, our findings suggest that MLT mitigates inflammation in cIECs by modulating the transcription of ZAP70 and CD40 through histone modifications, offering potential therapeutic insights for inflammatory bowel diseases.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0