Veterinary Sciences最新文献_第10页

Mechanisms of Transmission and Adaptation of tet(X4)-Positive IncHI1 Plasmids in XDR Escherichia coli from Pet Dogs: The Role of trhC, rsp, and the Tra1 Region. 宠物犬XDR大肠杆菌中tet（X4）阳性IncHI1质粒的传播和适应机制：trhC、rsp和Tra1区域的作用

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-28 DOI: 10.3390/vetsci12050418

Pengyun Ding, Qianqian Wang, Liangliang Wang, Mengxiang Zheng, Yiming Feng, Yakun Xu, Li Yuan, Gongzheng Hu, Yushan Pan, Dandan He

{"title":"Mechanisms of Transmission and Adaptation of tet(X4)-Positive IncHI1 Plasmids in XDR Escherichia coli from Pet Dogs: The Role of trhC, rsp, and the Tra1 Region.","authors":"Pengyun Ding, Qianqian Wang, Liangliang Wang, Mengxiang Zheng, Yiming Feng, Yakun Xu, Li Yuan, Gongzheng Hu, Yushan Pan, Dandan He","doi":"10.3390/vetsci12050418","DOIUrl":"10.3390/vetsci12050418","url":null,"abstract":"tet(X4)-positive IncHI1 plasmids are widely prevalent in various bacteria. To understand their transmission characteristics, we analyzed two extensively drug-resistant (XDR) Escherichia coli strains isolated from pet dog feces in Henan Province, China. Strain T28R harbored tet(X4)-positive IncHI1, IncF18:A-:B-, and mcr-1-positive IncI2 plasmids, while T16R carried tet(X4)-positive IncHI1, F16:A-:B-, and mcr-1-positive IncX4 plasmids. Four representative fusion plasmids, pT28R-F1, pT28R-F2, pT28R-F3, and pT16R-F1, in transconjugants were analyzed using WGS and PCR mapping. The results showed that IS26 from the IncF18:A-:B--plasmid attacked the conjugative transfer-associated genes trhc and rsp on the IncHI1 plasmid, generating pT28R-F1 and pT28R-F2. pT28R-F3 was generated through ISCro1- and ISCR2-mediated homologous recombination, deleting the Tra1 region of the IncHI1 plasmid. T16R-F1 emerged from ISCR2- and IS1B-mediated homologous recombination, losing transfer regions of parental plasmids. Notably, fusion plasmids lost the temperature sensitivity of the IncHI1 plasmid, with conjugation frequencies between 1.57 × 10-4 and 3.84 × 10-5 at 28 °C and 37 °C. The findings suggest that tet(X4)-positive IncHI1 plasmids could be mobilized with the assistance of conjugative helper plasmids and that fusion events enhance the adaptability of these plasmids, thus facilitating the spread of antibiotic resistance, posing a growing public health threat.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-Term Outcomes of Abdominal Surgeries Performed with Epidural Anaesthesia in Italian Calves. 意大利小牛硬膜外麻醉腹部手术的短期疗效。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-28 DOI: 10.3390/vetsci12050417

Gessica Giusto, Claudio Bellino, Anna Cerullo, Marco Gandini

{"title":"Short-Term Outcomes of Abdominal Surgeries Performed with Epidural Anaesthesia in Italian Calves.","authors":"Gessica Giusto, Claudio Bellino, Anna Cerullo, Marco Gandini","doi":"10.3390/vetsci12050417","DOIUrl":"10.3390/vetsci12050417","url":null,"abstract":"Abdominal surgery is widely described for several diseases in cattle. While in adults surgical procedures are generally performed in standing or in sternal recumbency, lateral or dorsal recumbency is preferred in calves. General anaesthesia is associated with several side effects, and epidural anaesthesia (EA) is a valid alternative anaesthetic protocol for several surgical approaches. Previous studies have already been published about abdominal surgery in calves for several diseases, but they are not focused on short-term outcomes. Therefore, there is a need to report a case series for calves that have undergone exploratory laparotomy under EA. General anaesthesia is limited in cattle due to its association with several complications, such as ruminal stasis, regurgitation and tympany, and alternative protocols need to be used. The aim of this study is to describe the surgical features and short-term outcomes of elective and emergency laparotomies in calves under EA. In more complex or longer-lasting surgery, with a median duration of 80 min, intravenous administration of xylazine was necessary. Short-term survival (defined as survival until discharge from the hospital) rate was 90.1%. The EA was sufficient to ensure adequate analgesia in most surgeries. This type of anaesthesia is easy to administer, with limited side effects. The results of this study support surgeons in decision-making in the event of pathologies requiring abdominal surgery.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory Testing to Inform Antimicrobial Use for Bovine Respiratory Disease: Perceptions of Canadian Feedlot Veterinarians. 实验室检测告知抗微生物药物用于牛呼吸道疾病：加拿大饲养场兽医的看法。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-27 DOI: 10.3390/vetsci12050409

Olufunto O Adewusi, Candace I J Nykiforuk, Cheryl L Waldner, Nathan E N Erickson, Sheryl P Gow, Simon J G Otto

{"title":"Laboratory Testing to Inform Antimicrobial Use for Bovine Respiratory Disease: Perceptions of Canadian Feedlot Veterinarians.","authors":"Olufunto O Adewusi, Candace I J Nykiforuk, Cheryl L Waldner, Nathan E N Erickson, Sheryl P Gow, Simon J G Otto","doi":"10.3390/vetsci12050409","DOIUrl":"10.3390/vetsci12050409","url":null,"abstract":"Timely, accurate, and reliable laboratory tools are important for antimicrobial stewardship (AMS) in food animal production. The objectives of this study were to (1) identify factors that influence live animal respiratory sample collection for laboratory testing and (2) describe the potential for integration of laboratory testing of samples from live animals into an AMS strategy for bovine respiratory disease (BRD) management in Canadian feedlots. Utilizing focused ethnography, virtual key informant interviews were conducted with eight feedlot veterinarians from Alberta, Ontario, and Saskatchewan, Canada. Thematic analysis revealed four themes: (1) lived experience of feedlot veterinarians with laboratory testing for BRD pathogens and antimicrobial resistance (AMR), (2) evidence-informed BRD management that integrates multiple data sources and their components, (3) organizational factors that affect the uptake and use of laboratory tests, and (4) the role of laboratory testing to support AMS in BRD management. While sample collection and laboratory testing are commonly used for BRD and AMR research and surveillance, they are not routinely applied in everyday BRD management. Veterinarians identified key challenges with laboratory testing, including turnaround time, the need for clear benefits, practical implementation, and effective communication with feedlot managers. Laboratory testing must provide valuable, actionable insights to encourage its adoption in Canadian feedlot operations. If successful, such testing could support AMS and be used to justify antimicrobial use if this were to become a regulatory or market requirement.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the Immunogenic Potential of ApxI and ApxII from Actinobacillus pleuropneumoniae: An Immunoinformatics-Driven Study on mRNA Candidates. 评估胸膜肺炎放线菌ApxI和ApxII的免疫原性：一项免疫信息学驱动的mRNA候选物研究

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-27 DOI: 10.3390/vetsci12050414

Yi Deng, Jia-Yong Chen, Yuhan Wang, Yu-Luo Wang, Jiale Liu, Zhiling Peng, Jiayu Zhou, Kun Lu, Xin Wen, Xizhu Chen, Siyu Pang, Dan Wang, Miaohan Li, Senyan Du, San-Jie Cao, Qin Zhao

{"title":"Evaluating the Immunogenic Potential of ApxI and ApxII from Actinobacillus pleuropneumoniae: An Immunoinformatics-Driven Study on mRNA Candidates.","authors":"Yi Deng, Jia-Yong Chen, Yuhan Wang, Yu-Luo Wang, Jiale Liu, Zhiling Peng, Jiayu Zhou, Kun Lu, Xin Wen, Xizhu Chen, Siyu Pang, Dan Wang, Miaohan Li, Senyan Du, San-Jie Cao, Qin Zhao","doi":"10.3390/vetsci12050414","DOIUrl":"10.3390/vetsci12050414","url":null,"abstract":"Porcine infectious pleuropneumonia (PCP) caused by Actinobacillus pleuropneumoniae (APP) leads to severe economic losses in swine production. Commercial vaccines offer limited cross-protection for the 19 serotypes, while APP mRNA vaccines remain unexplored. This study evaluated eight candidate APP proteins (ApxI-IV, OlmA, TbpB, GalT, and GalU) using immunobioinformatics tools, and their immunogenicity and cross-protection were assessed in a mouse model. The results revealed that ApxI and ApxII excel due to their stability, strong antigenicity, non-sensitization, and high immune receptor affinity. Compared to the PBS group, both ApxI and ApxII induced higher serum IgG, IL-2, IL-4, and IFN-γ levels. Following challenge with the two most prevalent APP strains in Mainland China, APP 5b and APP 1, the survival rates for ApxI (71.4% and 62.5%) and ApxII (75% and 71.4%) were measured, with notably reduced lung lesions and neutrophil infiltration. These findings highlight ApxI and ApxII's potential in mRNA vaccine development as a promising approach to overcome current vaccine limitations. Future research should focus on creating APP mRNA vaccines and testing their efficacy in swine. This study is the first to combine immunoinformatics with experimental validation for APP mRNA vaccine antigens, representing a novel contribution.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Active Immunization Against Inhibin Impaired Spermatogenesis, Plasma Luteinizing Hormone, Pituitary Prolactin mRNA, and Hypothalamic Vasoactive Intestinal Peptide mRNA Expressions in Yangzhou Ganders. 主动免疫抑制素对扬州公鹅精子发生、血浆黄体生成素、垂体催乳素mRNA和下丘脑血管活性肠肽mRNA表达的影响。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-27 DOI: 10.3390/vetsci12050413

Muhammad Faheem Akhtar, Muhammad Umar, Ejaz Ahmad, Mingxia Zhu, Ying Han, Changfa Wang

{"title":"Active Immunization Against Inhibin Impaired Spermatogenesis, Plasma Luteinizing Hormone, Pituitary Prolactin mRNA, and Hypothalamic Vasoactive Intestinal Peptide mRNA Expressions in Yangzhou Ganders.","authors":"Muhammad Faheem Akhtar, Muhammad Umar, Ejaz Ahmad, Mingxia Zhu, Ying Han, Changfa Wang","doi":"10.3390/vetsci12050413","DOIUrl":"10.3390/vetsci12050413","url":null,"abstract":"Inhibin (INH) plays a key role in the regulation of the reproductive performance of geese. It inhibits follicle-stimulating hormone (FSH) secretion from the anterior pituitary gland to regulate spermatogenesis. Immunization against INH in male geese leads to the production of antibodies to neutralize the INH activity that enhances testicular function and gonadotropin production. The objectives of the present research were to elaborate on the effects of inhibin (INH) immunization on testicular histology, plasma LH, pituitary PRL mRNA, and hypothalamic VIP mRNA expressions in Yangzhou ganders. A total of 60 birds were selected and divided into control (CON) and INH-immunized (INH-immunized) groups, having 30 in each group. In this experiment, the ganders were immunized with INH three times, and birds in the CON group were inoculated with bovine serum albumin (BSA). The analyzed data revealed that immunization against inhibin had no significant effects on improving the plasma concentration of LH hormone; however, significant effects were observed on the germ cell line, hypothalamic VIP mRNA, and pituitary PRL mRNA expressions. It is concluded that INH (INH) immunization is an effective tool to improve reproductive efficiency in Yangzhou ganders; however, INH immunization may harm pituitary PRL mRNA and hypothalamic mRNA expressions and LH plasma concentration. Seasonality played a vital impact on the hypothalamus-pituitary-gonadal (HPG) axis.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference Whole Genome Sequence Analyses and Characterization of a Novel Carnobacterium maltaromaticum Distinct Sequence Type Isolated from a North American Gray Wolf (Canis lupus) Gastrointestinal Tract. 从北美灰狼（Canis lupus）胃肠道分离的一种新型恶性肉杆菌的全基因组序列分析和鉴定。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-27 DOI: 10.3390/vetsci12050410

C Cristoph Klews, Jessika L Bryant, Jennifer McCabe, Ariel N Atchley, Thomas W Cousins, Maya Barnard-Davidson, Mark R Ackermann, Michael Netherland, Nur A Hasan, Peter A Jordan, Evan S Forsythe, Patrick N Ball, Bruce S Seal

{"title":"Reference Whole Genome Sequence Analyses and Characterization of a Novel Carnobacterium maltaromaticum Distinct Sequence Type Isolated from a North American Gray Wolf (Canis lupus) Gastrointestinal Tract.","authors":"C Cristoph Klews, Jessika L Bryant, Jennifer McCabe, Ariel N Atchley, Thomas W Cousins, Maya Barnard-Davidson, Mark R Ackermann, Michael Netherland, Nur A Hasan, Peter A Jordan, Evan S Forsythe, Patrick N Ball, Bruce S Seal","doi":"10.3390/vetsci12050410","DOIUrl":"10.3390/vetsci12050410","url":null,"abstract":"We hypothesize that bacteria isolated from free-ranging animals could potentially be useful for practical applications. To meet this objective a Gram-positive bacterium was isolated from the gastrointestinal (GI) tract of a Gray Wolf (Canis lupus) using Brucella broth with hemin and vitamin K (BBHK). By small ribosomal RNA (16S) gene sequencing the bacterium was initially identified as a novel Carnobacterium maltaromaticum strain. The bacterium could be propagated both anaerobically and aerobically and was both catalase/oxidase negative and negative by the starch hydrolysis as well as negative using lipase assays. The reference whole genome sequence (WGS) was obtained using both Illumina and Nanopore sequencing. The genome assembly was 3,512,202 bp in length, encoding core bacterial genes with a GC% content of 34.48. No lysogenic bacteriophage genes were detected, although the genome harbors genes for the expression of bacteriocin and other secondary metabolites with potential antimicrobial properties. Multilocus sequence typing (MLST), WGS phylogenetics, average nucleotide identity (ANI), and single nucleotide polymorphism (SNP) analyses of the isolate's genome indicate this bacterium is a newly identified Carnobacterium maltaromaticum sequence type (ST). Members of the Carnobacteria have anti-listeria activities, highlighting their potential functional properties. Consequently, the isolate could be a potential probiotic for canids and this is the first report on an axenic C. maltaromaticum culture from the genus Canis.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Codonopsis pilosula Polysaccharides Exert Antiviral Effect Through Activating Immune Function in a Macrophage Model of Bovine Viral Diarrhea Virus Infection. 党参多糖在牛病毒性腹泻病毒感染巨噬细胞模型中通过激活免疫功能发挥抗病毒作用。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-27 DOI: 10.3390/vetsci12050415

Xiaowei Feng, Lei Wang, Jingyan Zhang, Haipeng Feng, Xiaoliang Chen, Junyan Wang, Mingxian Shi, Kang Zhang, Jianxi Li

{"title":"Codonopsis pilosula Polysaccharides Exert Antiviral Effect Through Activating Immune Function in a Macrophage Model of Bovine Viral Diarrhea Virus Infection.","authors":"Xiaowei Feng, Lei Wang, Jingyan Zhang, Haipeng Feng, Xiaoliang Chen, Junyan Wang, Mingxian Shi, Kang Zhang, Jianxi Li","doi":"10.3390/vetsci12050415","DOIUrl":"10.3390/vetsci12050415","url":null,"abstract":"Bovine viral diarrhea (BVD) is a common viral disease in cattle that causes huge economic losses in naïve herds that are introduced to bovine viral diarrhea virus (BVDV). Currently, there are no available anti-BVDV drugs due to the variety and mutability of strains; therefore, developing new anti-BVDV drugs is of great significance. The aim of this study was to investigate the anti-BVDV effects and immunomodulatory activities of Codonopsis pilosula polysaccharides (CPPs) in BVDV-infected bovine macrophage (BoMac) cells. CPPs directly inactivated BVDV particles and intervened in BVDV absorption process. The immunity suppression resulting from BVDV in BoMac cells was restored by CPPs, as was verified by phagocytosis increase, the expression up-regulation of cell surface co-stimulatory molecules (CD40, CD80, and CD86), and antigen-presenting function recovery. Furthermore, the expressions of cytokines including TNF-α, IFN-γ, IL-6, IL-18, IL-1β, Caspase-3, Bim, and Bcl-xL at the mRNA and proteins levels were modulated, and a reduction in the apoptosis rate was observed, which demonstrates that CPPs attenuated inflammation and apoptosis induced by BVDV. Collectively, our findings reveal new pharmacological properties of CPPs, which exert anti-BVDV efficacy and regulate immune injury induced by BVDV in BoMac cells, indicating that CPPs are a potential option for BVDV prevention in clinical application.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Cottonseed Meal in Feeding Yellow-Feathered Broilers: Effects on Performance Parameters, Digestibility and Meat Quality. 饲用棉籽粕对黄羽肉鸡生产性能、消化率和肉品质的影响。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-27 DOI: 10.3390/vetsci12050416

Xiaohang Nie, Xiahan Wei, Weidong Niu, Fengming Li, Jiang Yuan, Gang Lv, Yong Chen, Jiancheng Liu

{"title":"Use of Cottonseed Meal in Feeding Yellow-Feathered Broilers: Effects on Performance Parameters, Digestibility and Meat Quality.","authors":"Xiaohang Nie, Xiahan Wei, Weidong Niu, Fengming Li, Jiang Yuan, Gang Lv, Yong Chen, Jiancheng Liu","doi":"10.3390/vetsci12050416","DOIUrl":"10.3390/vetsci12050416","url":null,"abstract":"This study investigated the effects of replacing soybean meal (SBM) with high-protein cottonseed meal (CSM) and de-phenolized cottonseed meal (DPCSM) on growth performance, apparent nutrient digestibility, serum biochemical parameters, slaughter traits, and meat quality in yellow-feathered broilers. A total of 5760 one-day-old male Liangfeng Hua broilers were randomly divided into five groups with eight replicates per group. The control group was fed a corn-SBM diet (SBM group), while the CSM50, CSM100, DPCSM50, and DPCSM100 groups replaced 50% and 100% of the equivalent protein from SBM with CSM and DPCSM, respectively. Compared to the control group, the CSM50 and DPCSM50 groups showed no significant negative effect on growth performance; however, dietary calcium digestibility was significantly reduced on day 21. Furthermore, CSM100 and DPCSM100 adversely impacted growth performance, significantly reducing crude fat digestibility and increasing serum urea nitrogen levels on day 42. The broilers in these groups also had reduced pre- and post-slaughter body weights, while those in the CSM50 and DPCSM100 groups exhibited decreased half-clearance rates. In addition, the CSM100 group had significantly increased pectoral muscle drip loss and a* (24 h) values. pH values measured at 45 min and 24 h were significantly elevated in the DPCSM50 and DPCSM100 groups. In conclusion, the replacement of SBM with CSM and DPCSM in yellow-feathered broiler diets should be limited to 50%.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Enterococcus faecium Kimate-X on Reducing Stress in Dogs Through Gut Microbiota Modulation. 屎肠球菌Kimate-X通过调节肠道菌群减轻狗应激的影响。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-27 DOI: 10.3390/vetsci12050412

Rui Zhang, Wanjin Hu, Saiwei Zhong, Weiyang Chen, Meiru Chen, Qinghua Yu

{"title":"Impact of Enterococcus faecium Kimate-X on Reducing Stress in Dogs Through Gut Microbiota Modulation.","authors":"Rui Zhang, Wanjin Hu, Saiwei Zhong, Weiyang Chen, Meiru Chen, Qinghua Yu","doi":"10.3390/vetsci12050412","DOIUrl":"10.3390/vetsci12050412","url":null,"abstract":"Stress in dog breeding leads to significant physiological and psychological burdens, including anxiety, reduced appetite, weakened immune function, gut microbiota imbalance, and even death. Currently, there are various pharmacological interventions for stress management, but few focus on gut health. This study evaluates the potential of a novel strain, Enterococcus faecium Kimate-X, in alleviating transport stress and improving gut health in dogs, providing an alternative to traditional pharmacological treatments. In vitro experiments showed that Kimate-X significantly enhanced the activities of superoxide dismutase (SOD) and catalase (CAT) while reducing the levels of malondialdehyde (MDA) and tumor necrosis factor-α (TNF-α) in RAW 264.7 macrophage cells. In vivo, dogs supplemented with Kimate-X exhibited significantly lower cortisol levels after transport, indicating reduced stress. Metagenomic analysis revealed increased gut microbiota diversity and higher concentrations of short-chain fatty acids (acetate, propionate, and butyrate) in fecal samples. This study systematically uncovers the mechanism by which Enterococcus faecium Kimate-X alleviates transport stress through modulation of the gut microbiota. These findings provide new scientific evidence supporting the use of probiotics as a novel approach to stress management in animals.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12115767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reighardia sternae Infection and Associated Lesions in a Yellow-Legged Gull (Larus michahellis) in Italy. 意大利一只黄腿鸥（Larus michahellis）的胸骨雷氏体感染及相关病变。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2025-04-27 DOI: 10.3390/vetsci12050411

Renato Ceccherelli, Valentina Virginia Ebani, Stefano Pesaro, Giacomo Rossi, Stefania Perrucci

{"title":"Reighardia sternae Infection and Associated Lesions in a Yellow-Legged Gull (Larus michahellis) in Italy.","authors":"Renato Ceccherelli, Valentina Virginia Ebani, Stefano Pesaro, Giacomo Rossi, Stefania Perrucci","doi":"10.3390/vetsci12050411","DOIUrl":"10.3390/vetsci12050411","url":null,"abstract":"Reighardia sternae is a pentastomid parasitising the respiratory system of larids and skuas, the pathological significance of which is still poorly understood. In this study, pentastomes found in the interclavicular air sac of a deceased and necropsied yellow-legged gull (Larus michahellis) were identified by parasitological and molecular methods and associated gross and histopathological lesions were evaluated. After morphological evaluation and molecular characterisation, the species R. sternae was identified. This is the first report of R. sternae infecting L. michahellis in Italy. Gross pathology showed generalised aerosacculitis, parabronchial edema, and enlargement of the heart, liver, and spleen. The systemic lesions found at histopathological examination were indicative of chronic cardiocirculatory failure, which led to the death of the gull. Moreover, inflammation was present throughout the lungs and air sacs in the form of some granulomas and diffuse small nodular aggregates rich in lymphocytes. The remnants of a parasite were observed within a granulomatous lesion in the lung, suggesting a primary host response to the parasite. These latter findings may suggest that the migration of R. sternae was the cause of the pathological granulomatous pictures in the examined yellow-legged gull.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0