Veterinary Sciences最新文献_第9页

Anti-Shrinkage Technique for Feline Skin Samples. 猫科动物皮肤样本防缩技术

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-15 DOI: 10.3390/vetsci11100507

Caterina Kiniger, Ligita Zorgevica-Pockevica, Simona Vincenti

{"title":"Anti-Shrinkage Technique for Feline Skin Samples.","authors":"Caterina Kiniger, Ligita Zorgevica-Pockevica, Simona Vincenti","doi":"10.3390/vetsci11100507","DOIUrl":"https://doi.org/10.3390/vetsci11100507","url":null,"abstract":"Surgical resection of subcutaneous neoplasms with clear margins is crucial for preventing local recurrence and avoiding adjuvant treatments. However, the evaluation of surgical margins often differs significantly from the histopathological assessment due to tissue shrinkage, which can result in inaccurate therapeutic assessments and unreliable patient prognoses. In this study, ten feline cadavers were utilized. Six 50 mm diameter specimens were collected from three regions (thorax, flank, femur) and measured at three time points: T0 (excision time), T1 (10 min after incision), and T2 (at least 48 h after sample collection and formalin fixation). Samples in the study group were stretched and fixed on a cork plate with pinpoint needles after excision to restore their original dimensions. All specimens exhibited a similar trend. After 48 h of formalin fixation, the control specimens showed significant shrinkage, with a reduction of 25.73% in radius and 26.32% in diameter. In contrast, the study specimens demonstrated minimal changes, with a radius reduction of -0.28% and no change in diameter. The results indicate that all feline skin specimens experienced significant shrinkage of approximately one-quarter from their pre-incisional size. Stretching and pinning the excised tissues allowed for the restoration and maintenance of original dimensions even after formalin fixation. This technique represents a valid and practical approach to minimize tissue shrinkage.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Supplementation Plans and Frequency on Performance and Metabolic Responses of Grazing Pregnant Beef Heifers. 补饲计划和频率对放牧怀孕母牛的性能和代谢反应的影响

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-15 DOI: 10.3390/vetsci11100506

Luciano Prímola de Melo, Luciana Navajas Rennó, Edenio Detmann, Mário Fonseca Paulino, Ronaldo Gomes da Silva Júnior, Román Maza Ortega, Deilen Sotelo Moreno

{"title":"Effect of Supplementation Plans and Frequency on Performance and Metabolic Responses of Grazing Pregnant Beef Heifers.","authors":"Luciano Prímola de Melo, Luciana Navajas Rennó, Edenio Detmann, Mário Fonseca Paulino, Ronaldo Gomes da Silva Júnior, Román Maza Ortega, Deilen Sotelo Moreno","doi":"10.3390/vetsci11100506","DOIUrl":"https://doi.org/10.3390/vetsci11100506","url":null,"abstract":"Our objective was to evaluate the effects of protein supplementation frequency and supplementation plans during gestation on productive performance and metabolic responses of grazing super-precocious Nellore heifers. Thirty-five pregnant Nellore heifers were used in a completely randomized design in a 2 × 2 factorial arrangement with an additional control group. The factors were the following: 1. Frequency of supplementation: (a) Daily (seven times per week), (b) Infrequent (three times per week); 2. Supplementation plans: (a) Constant, (b) Increasing. A control group with no supplementation was included. Two digestibility trials were carried out on the 40th and 130th experimental days. Productive performance and body composition were evaluated in the prepartum and postpartum periods. Blood samples were collected at -114, -113, -15, -14, +15, +30, and +45 days relative to calving for measuring metabolic status. Supplementation (daily and infrequent) increased the intake of total dry matter (DM) (p ≤ 0.004), average daily gain (p < 0.001), and body weight at calving (p = 0.008) at prepartum. However, frequency or supplementation plan did not alter (p > 0.17) the intake of total DM and forage DM. There was an effect (p ≤ 0.02) of the supplementation plan on subcutaneous thickness fat in the prepartum period and albumin and non-esterified fatty acid concentrations (p < 0.004) in the postpartum period. Nonetheless, frequency and supplementation plans did not affect (p ≥ 0.10) heifers' productive performance during the postpartum period. In conclusion, protein supplementation frequency and supplementation plan during beef heifers' gestation do not negatively impact their productive performance or metabolic responses.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Molecular Identification and Prevalence of Sarcocystis spp. in Sheep Intended for Human Consumption in Shanxi Province, China. 中国山西省供人类食用的绵羊中沙眼衣原体的首次分子鉴定和流行情况。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-14 DOI: 10.3390/vetsci11100504

Yu Kang, Xin-Sheng Lu, Yuan-Hui He, Chen Wang, Ze-Xuan Wu, Lu Wang, Xiao-Jing Wu, Jun-Jie Hu, Xing-Quan Zhu

{"title":"First Molecular Identification and Prevalence of Sarcocystis spp. in Sheep Intended for Human Consumption in Shanxi Province, China.","authors":"Yu Kang, Xin-Sheng Lu, Yuan-Hui He, Chen Wang, Ze-Xuan Wu, Lu Wang, Xiao-Jing Wu, Jun-Jie Hu, Xing-Quan Zhu","doi":"10.3390/vetsci11100504","DOIUrl":"https://doi.org/10.3390/vetsci11100504","url":null,"abstract":"Sarcocystis species are intracellular coccidian protozoans that can infect a range of animals and humans and cause public health problems and economically significant losses. Sarcocystosis in sheep (Ovis aries) can cause abortion, neurological symptoms, and even death and results in significant economic losses to the livestock industry. To date, however, it is yet unknown whether sheep in Shanxi Province, north China, are infected with Sarcocystis spp. The purpose of this study was to investigate the prevalence of Sarcocystis spp. in sheep in Shanxi Province. Thus, 582 muscle samples of sheep were purchased from farmers' markets from ten representative counties in Shanxi Province, north China, and examined for the presence and prevalence of Sarcocystis spp. by PCR amplification of the mitochondrial cytochrome c oxidase subunit I (cox1) gene. Of the examined 582 mutton samples, 197 samples (33.85%) were Sarcocystis-positive and were sequenced. Of the obtained 197 cox1 sequences, 196 sequences showed nucleotide similarity of 98.56-99.81% with those of S. tenella, and the remaining one cox1 sequence showed nucleotide similarity of 99.71% with that of S. arieticanis. Two representative cox1 sequences of S. tenella (accession nos. PQ189447 and PQ189448) have 99.52% and 99.61% identity with S. tenalla (KC209725) and S. tenalla (MK419984), respectively. The sequence of S. arieticanis (accession no. PQ165949) obtained in this study has 99.71% identity with S. arieticanis (MK419975). This present study documents the occurrence and prevalence of Sarcocystis spp. in sheep in Shanxi Province for the first time, which enriches the data on the distribution of Sarcocystis spp. in sheep in China and has implications for the control of sheep sarcocystosis.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142516683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and Associated Risk Factors of Helminth Infections in the Digestive Tract of Camels in Xinjiang, China. 中国新疆骆驼消化道螺旋虫感染的流行率及相关风险因素。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-14 DOI: 10.3390/vetsci11100503

Yi Zhang, Danchen Aaron Yang, Min Yang, Mengjie Pi, Yang Zhang, Zhanqiang Su

{"title":"Prevalence and Associated Risk Factors of Helminth Infections in the Digestive Tract of Camels in Xinjiang, China.","authors":"Yi Zhang, Danchen Aaron Yang, Min Yang, Mengjie Pi, Yang Zhang, Zhanqiang Su","doi":"10.3390/vetsci11100503","DOIUrl":"https://doi.org/10.3390/vetsci11100503","url":null,"abstract":"Camels, vital to economies in Asia, Africa, and the Arabian Peninsula, have been domesticated for over 4000 years. They thrive in arid regions like Xinjiang, China, but face challenges from internal and external parasites, particularly gastrointestinal parasites, which impact health, meat and milk quality, and production efficiency. This study investigates the prevalence of gastrointestinal helminth infections in camels from five major regions in Xinjiang. We collected 435 fresh fecal samples and used the saturated saline flotation method and McMaster's method for detection. Molecular examination followed. The overall prevalence was 18.2% (95% confidence interval [CI]: 14.7-22.2%), with Urumqi showing the highest prevalence (29%, 95% CI: 23.4-35.1%) compared to other regions (odds ratio [OR]: 4.62, 95% CI: 2.63-8.41%). Younger camels (≤3 years old) were more likely infected by the parasites after adjusting for the region differences (OR: 10.53, 5.12-24.65%). However, we found no evidence that the prevalence was different between male and female camels. PCR analysis identified Trichostrongylus spp., Chabertia ovina, and Haemonchus contortus as predominant parasites, with observed co-infections indicating a complex parasitic landscape. The findings provide essential epidemiological data for effective parasite control strategies.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cow Placenta Extract Ameliorates Cyclophosphamide-Induced Intestinal Damage by Enhancing the Intestinal Barrier, Improving Immune Function, and Restoring Intestinal Microbiota. 牛胎盘提取物通过增强肠道屏障、改善免疫功能和恢复肠道微生物群来减轻环磷酰胺引起的肠道损伤

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-14 DOI: 10.3390/vetsci11100505

Yuquan Zhao, Zeru Zhang, Anguo Tang, Zhi Zeng, Weijian Zheng, Yuxin Luo, Yixin Huang, Xinyi Dai, Wei Lu, Lei Fan, Liuhong Shen

{"title":"Cow Placenta Extract Ameliorates Cyclophosphamide-Induced Intestinal Damage by Enhancing the Intestinal Barrier, Improving Immune Function, and Restoring Intestinal Microbiota.","authors":"Yuquan Zhao, Zeru Zhang, Anguo Tang, Zhi Zeng, Weijian Zheng, Yuxin Luo, Yixin Huang, Xinyi Dai, Wei Lu, Lei Fan, Liuhong Shen","doi":"10.3390/vetsci11100505","DOIUrl":"https://doi.org/10.3390/vetsci11100505","url":null,"abstract":"Immunosuppression undermines intestinal barrier integrity. Cow placenta extract (CPE) primarily consists of active peptides with immunomodulatory and antioxidant effects. This study aimed to examine the preventive effect of CPE against intestinal damage induced by cyclophosphamide (Cy) in immunosuppressed mice. Thirty-six mice were randomly allocated into three groups: control group (C), model group (M), and treatment group (CPE). The mice in the CPE group were provided with 1500 mg/kg/day of CPE via gavage. In the last 3 days, mice in the groups M and CPE received intraperitoneal injections of 80 mg/kg/day of Cy. The results showed that CPE improved intestinal barrier function by decreasing serum d-Lactate (D-LA) levels and diamine oxidase (DAO) activity, while elevating the relative expression of Occludin, zonula occludens-1 (ZO-1), and mucin-2 (MUC-2) mRNA. Additionally, CPE improved the immune organ index and elevated the levels of secretory immunoglobulin A (sIgA), superoxide dismutase (SOD), interleukin-1beta (IL-1β), interleukin-4 (IL-4), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF-α) in the intestine, thereby enhancing intestinal mucosal immune function. Furthermore, CPE improved the diversity of intestinal microbiota and increased the abundance of Candidatus_Saccharimonas, Psychrobacter, and Enterorhabdus, which promoted the proper functioning of the intestines. These findings suggest that CPE effectively ameliorates Cy-induced intestinal damage by enhancing the intestinal barrier, improving immune function, and restoring intestinal microbiota.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rabies Vaccination for Sheep and Goats: Influence of Booster on Persistence of Antibody Response. 绵羊和山羊狂犬病疫苗接种：增强剂对抗体反应持续性的影响。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-13 DOI: 10.3390/vetsci11100502

Sarah Weyl Feinstein, Shiri Novak, Marina Eyngor, Yaniv Lavon, Boris Yakobson

{"title":"Rabies Vaccination for Sheep and Goats: Influence of Booster on Persistence of Antibody Response.","authors":"Sarah Weyl Feinstein, Shiri Novak, Marina Eyngor, Yaniv Lavon, Boris Yakobson","doi":"10.3390/vetsci11100502","DOIUrl":"https://doi.org/10.3390/vetsci11100502","url":null,"abstract":"Infrequent rabies cases occur in Israel, endangering humans and animals. While dogs receive mandatory vaccinations, farm animals are vaccinated voluntarily. However, optimal vaccination protocol for small ruminants is lacking. The aim of this study was to test the immunological responses to the rabies vaccine, with or without a booster, in sheep and goats; 70 ewes and 49 does participated in the trial. Following the first vaccine, 88% of the ewes and 100% of the does had a sufficient level of rabies antibodies (>0.5 IU/mL) 30 days post-vaccination. A year later, 82% of the ewes that had received a booster dose remained protected, whereas 46% of the non-boosted ewes had a sufficient antibody level. For does, 83% of those receiving a booster maintained sufficient antibody levels 1 year later; 80% of the non-boosted does remained protected, demonstrating no significant contribution of the booster dose in this group of goats. However, while the initial immunological response of the does was higher, the change in response between 1 month and 12 months post-vaccination differed significantly between species, with a greater titer reduction in the does. Differential immunological responses between individuals and between species warrant longer-term studies to recommend a proper vaccine protocol for each species.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Antitumor Effect of Oils Rich in CBD and THC Cannabis Extract in Canine Prostate Carcinoma Cell Lines. 富含 CBD 和 THC 大麻提取物的精油对犬前列腺癌细胞株的体外抗肿瘤作用。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-13 DOI: 10.3390/vetsci11100501

Luís Gustavo Ramos de Moraes Calheiros, Giovana Pedro, Thayna Oliveira da Silva, Rogério Martins Amorim, Carlos Eduardo Fonseca Alves, Renée Laufer-Amorim

{"title":"In Vitro Antitumor Effect of Oils Rich in CBD and THC Cannabis Extract in Canine Prostate Carcinoma Cell Lines.","authors":"Luís Gustavo Ramos de Moraes Calheiros, Giovana Pedro, Thayna Oliveira da Silva, Rogério Martins Amorim, Carlos Eduardo Fonseca Alves, Renée Laufer-Amorim","doi":"10.3390/vetsci11100501","DOIUrl":"https://doi.org/10.3390/vetsci11100501","url":null,"abstract":"Prostate cancer is one of the leading causes of cancer-related deaths worldwide, even when diagnosed at an early stage in humans and dogs. Dogs have a significant incidence of spontaneous prostate cancer, which is highly similar to human androgen-independent prostate cancer and represents a valuable model for comparative studies. Cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) are the two main cannabinoids extracted from Cannabis sativa and have demonstrated antiproliferative and anti-invasive properties in different tumor types. In this study, CBD or THC-rich extracts inhibited the proliferation of two canine prostatic carcinoma cell lines, PC1 and PC2, showing an IC50 of 3.43 and 3.57 μM for CBD rich extracts, and 4.90 and 4.48 μM THC rich extracts, respectively. Cell death was also observed with both Annexin V and Propidium iodide staining for the canine cell lines. These results provide new information concerning the use of rich oil in canine PC and open a promising opportunity for further in vitro and in vivo studies to establish the mechanisms of action of these compounds using dogs as a natural model for prostatic carcinoma.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142516684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Partial Sequence Analysis of Commercial Peste des Petits Ruminants Vaccines Produced in Africa. 非洲生产的小反刍兽疫商用疫苗的部分序列分析。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-13 DOI: 10.3390/vetsci11100500

Boubacar Barry, Yebechaye Tessema, Hassen Gelaw, Cisse Rahamatou Moustapha Boukary, Baziki Jean de Dieu, Melesse Ayelet Gelagay, Ethel Chitsungo, Richard Rayson Sanga, Gbolahanmi Akinola Oladosu, Nick Nwankpa, S Charles Bodjo

{"title":"Partial Sequence Analysis of Commercial Peste des Petits Ruminants Vaccines Produced in Africa.","authors":"Boubacar Barry, Yebechaye Tessema, Hassen Gelaw, Cisse Rahamatou Moustapha Boukary, Baziki Jean de Dieu, Melesse Ayelet Gelagay, Ethel Chitsungo, Richard Rayson Sanga, Gbolahanmi Akinola Oladosu, Nick Nwankpa, S Charles Bodjo","doi":"10.3390/vetsci11100500","DOIUrl":"https://doi.org/10.3390/vetsci11100500","url":null,"abstract":"Peste des petits ruminants virus (PPRV), which is the only member of the Morbillivirus caprinae species and belongs to the genus Morbillivirus within the Paramyxoviridae family, causes the highly contagious viral sickness \"Peste des petits ruminants (PPR).\" PPR is of serious economic significance for small ruminant production, particularly in Africa. Control of this critical disease depends highly on successful vaccination against the PPRV. An in-depth understanding of the genetic evolution of the live-attenuated PPR vaccine Nigeria 75/1 strain used in Africa is essential for the successful eradication of this disease by 2030. Therefore, this study investigated the possible genetic evolution of the PPR vaccine produced by various African laboratories compared with the master seed available at AU-PANVAC. RT-PCR was performed to amplify a segment of the hypervariable C-terminal part of the nucleoprotein (N) from commercial batches of PPR vaccine Nigeria 75/1 strain. The sequences were analyzed, and 100% nucleotide sequence identity was observed between the master seed and vaccines produced. The results of this study indicate the genetic stability of the PPR vaccine from the Nigeria 75/1 strain over decades and that the vaccine production process used by different manufacturers did not contribute to the emergence of mutations in the vaccine strain.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Anogenital Distance and Anti-Müllerian Hormone Plasmatic Concentration as Potential Phenotypes to Predict Reproductive Performance in Holstein Heifers. 将肛门距离和抗缪勒氏管激素浆液浓度作为预测荷斯坦母牛繁殖性能的潜在表型进行评估。

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-12 DOI: 10.3390/vetsci11100495

Lucía Vidal, Jacobo Álvarez, Uxía Yáñez, Juan Caínzos, Rodrigo Muíño, Juan J Becerra, Ana I Peña, Luis A Quintela, Pedro G Herradón

{"title":"Evaluation of Anogenital Distance and Anti-Müllerian Hormone Plasmatic Concentration as Potential Phenotypes to Predict Reproductive Performance in Holstein Heifers.","authors":"Lucía Vidal, Jacobo Álvarez, Uxía Yáñez, Juan Caínzos, Rodrigo Muíño, Juan J Becerra, Ana I Peña, Luis A Quintela, Pedro G Herradón","doi":"10.3390/vetsci11100495","DOIUrl":"https://doi.org/10.3390/vetsci11100495","url":null,"abstract":"Anogenital distance (AGD) is a marker of the degree of prenatal exposure to androgens in multiple species, and it has been suggested that there is an inverse association between AGD and fertility. Therefore, the aim of this study was to determine the usefulness of AGD and anti-Müllerian hormone (AMH) concentrations, an indirect marker of the follicular population, as predictors of future reproductive potential in Holstein cattle. The AGD was measured in 566 females from 9 dairy farms in Galicia (Spain). A group of 172 females underwent a second measurement 9 months after. Additionally, data on the age at first insemination (1stAI age), number of AI (AI-PREG), age at first pregnancy (1stPREG age), age at first calving (1stCAL age), and calving-pregnancy (CAL-PREG) and calving-calving (CAL-CAL) intervals were collected. Blood samples were collected from 80 heifers to determine AMH concentrations. Our results showed that AGD varied minimally with age, and that cows with short AGD had earlier 1stAI age, 1stPREG age, and 1stCAL age (p < 0.05) than cows with long AGD. No significant differences were observed for the CAL-PREG and CAL-CAL intervals. Additionally, no significant association was found between AMH concentration and AGD or reproductive parameters. Consequently, the results suggest the possibility of using AGD as a marker of future reproductive performance in Holstein heifers. However, there was insufficient evidence to associate AMH concentrations and reproductive performance, underlining the need for further studies with larger sample sizes.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Balance Assessment on a Modified Posturomed Platform in Healthy Dogs. 健康犬在改良体位平台上的平衡评估

IF 2 2区农林科学

Veterinary Sciences Pub Date : 2024-10-12 DOI: 10.3390/vetsci11100498

Viola Wolszky, Yury Zablotski, Andrea Fischer, Susanne Lauer

{"title":"Balance Assessment on a Modified Posturomed Platform in Healthy Dogs.","authors":"Viola Wolszky, Yury Zablotski, Andrea Fischer, Susanne Lauer","doi":"10.3390/vetsci11100498","DOIUrl":"https://doi.org/10.3390/vetsci11100498","url":null,"abstract":"Reliable, standardized balance tests for dogs are not available yet. The purpose of this study was to investigate the reliability of static and dynamic posturography in healthy dogs. Healthy dogs (n = 20) were positioned with four paws longitudinally and with the forepaws only transversely on a modified pressure-sensitive balance platform (Posturomed-FDM-JS, Zebris, Isny, Germany). Three static and dynamic posturographic trials were recorded (recording duration: 20 s) and repeated after 7-14 days. Center of pressure (COP) parameters COP-path-length (PL; mm), 95% COP-confidence-ellipse-area (CEA; mm2), and COP-average-velocity (AV; mm/s) were calculated for the first steady-state 5 s intervals of each trial. The reliability of COP parameters was assessed with robust linear mixed effects models with nested random effects of patient and trial. The training effect was analyzed using Cohen's d. For static posturography, PL, CEA, and AV did not differ significantly between time points; CEA had the highest reliability (p = 0.92). For dynamic posturography, AV and PL differed significantly between time points (AV: p ≤ 0.043; PL: p ≤ 0.045). Slight training effects were observed for transverse positioning (Cohen's d: PL 0.65; AV 0.267) and moderate training effects for longitudinal positioning (Cohen's d: PL: 0.772; AV: 0.783). This study showed that static posturography on a modified Posturomed-balance platform was reliable in healthy dogs but indicated training effects during dynamic posturography.","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0