Effects of Intra-uterine Ceftiofur on the Equine Uterine Microbiome.

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Kalie F Beckers, Chin-Chi Liu, Viviane C L Gomes, Christopher J Schulz, Gary W Childers, Carleigh E Fedorka, Jenny L Sones
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Abstract

Antimicrobial therapy is a mainstay for treating reproductive diseases, including endometritis. Ceftiofur, a third-generation cephalosporin, is a common antibiotic used to treat equine bacterial endometritis. It is also routinely given empirically as an intra-uterine (IU) infusion in broodmare practice. We hypothesized that ceftiofur IU would disrupt the resident microbial community within the healthy uterus of mares. To test our hypothesis, eight university-owned mares were selected for characterization of the estrual uterine microbiome before and after IU ceftiofur. Double-guarded swabs of the estrual endometrium were taken before and 3 days after both IU saline and ceftiofur in a crossover design. Isolation of DNA from endometrial swabs was performed, followed by amplification of the V4 region of the 16S rRNA gene by Illumina Miseq sequencing to examine core bacterial communities present before and after ceftiofur. The uterine microbial composition of sham and ceftiofur-treated mares was not significantly different as measured by beta diversity. The only notable difference was a lower abundance of Christensenellaceae_R-7_group after ceftiofur (0.14 ± 1.05% vs. 2.89 ± 1.07% control; p = 0.0428). In conclusion, three-day treatment of ceftiofur did not change the microbial composition acutely within the mare uterus when sampled directly after treatment. Ceftiofur may have a long-term effect on the uterine microbiome, which may require sampling several weeks post treatment. In conclusion, ceftiofur does not change the healthy uterine microbiome acutely during estrus and but should still be used judiciously.

子宫内注射头孢替福对马子宫微生物群的影响。
抗菌治疗是治疗包括子宫内膜炎在内的生殖疾病的主要方法。Ceftiofur是第三代头孢菌素,是治疗马细菌性子宫内膜炎的常用抗生素。它也是常规给予经验性子宫内(IU)输液在母体实践。我们假设头孢替弗IU会破坏母马健康子宫内的常驻微生物群落。为了验证我们的假设,我们选择了八匹大学拥有的母马,对服用头孢替福前后的发情子宫微生物群进行了表征。在交叉设计中,在使用IU生理盐水和头孢替福治疗前和治疗后3天,采用双保护拭子采集排卵子宫内膜。从子宫内膜拭子中分离DNA,然后通过Illumina Miseq测序扩增16S rRNA基因的V4区,检测头孢替福前后存在的核心细菌群落。经β多样性测定,假药和头孢替弗尔治疗的母马子宫微生物组成无显著差异。唯一显著差异是头孢替福后Christensenellaceae_R-7_group丰度较低(0.14±1.05% vs. 2.89±1.07%;p = 0.0428)。综上所述,治疗后直接取样的母马子宫内的微生物组成,头孢替福治疗三天不会发生剧烈变化。头孢替福可能对子宫微生物群有长期影响,可能需要在治疗后几周取样。综上所述,在发情期间,头孢替福对健康的子宫微生物群没有明显的改变,但仍应谨慎使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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