LPS-Induced Intracellular Complement 3 Activation Regulated ATP Production in Yak Rumen Epithelial Cells.

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Qiang Han, Qiqi Zhang, Duoting Wu, Zihan Yang, Jinyang Huang, Zhisheng Wang, Huawei Zou, Quanhui Peng, Yukun Meng, Yahui Jiang, Jianxin Xiao, Rui Hu
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Abstract

This study aimed to investigate whether intracellular complement 3 (C3) activation regulates ATP production in yak rumen epithelial cells under inflammatory conditions and its potential mechanism. An in vitro inflammation model was established by stimulating yak rumen epithelial cells with lipopolysaccharide (LPS). Then, protease inhibitors targeting C3 activation enzymes were added. Additionally, to explore the downstream signaling pathway, exogenous C3a and the C3a receptor (C3aR) inhibitor C3aRY were applied to the inflammation model. After treatment with different concentrations of LPS, the gene expression levels and concentrations of pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6 were significantly up-regulated (p < 0.05), while a significant reduction in cellular ATP levels was observed (p < 0.05), along with a significant reduction in mitochondrial membrane potential (p < 0.05). After treating the inflammation model with different protease inhibitors, the ATP content and gene expression of the ATP synthase subunit ATP5A were significantly increased (p < 0.05). Exogenous addition of the C3aR inhibitor C3aRY in the inflammation model exhibited a significant increase in ATP content and ATP5A gene expression (p < 0.05) when compared to the inflammation model. These results demonstrated that intracellular C3 activation inhibited ATP production in yak rumen epithelial cells under inflammatory conditions, likely through C3a-C3aR signaling and the cAMP/PKA pathway.

lps诱导的细胞内补体3激活调节牦牛瘤胃上皮细胞ATP的产生
本研究旨在探讨炎症条件下细胞内补体3 (C3)激活是否调控牦牛瘤胃上皮细胞ATP的产生及其可能的机制。采用脂多糖(LPS)刺激牦牛瘤胃上皮细胞,建立了体外炎症模型。然后加入靶向C3活化酶的蛋白酶抑制剂。此外,为了探索下游信号通路,我们将外源性C3a和C3a受体(C3aR)抑制剂C3aRY应用于炎症模型。不同浓度LPS处理后,细胞中促炎因子TNF-α、IL-1β、IL-6等基因表达水平和浓度均显著上调(p < 0.05),细胞ATP水平显著降低(p < 0.05),线粒体膜电位显著降低(p < 0.05)。用不同蛋白酶抑制剂处理炎症模型后,ATP合成酶亚基ATP5A的ATP含量和基因表达量均显著升高(p < 0.05)。在炎症模型中外源性添加C3aR抑制剂C3aRY,与炎症模型相比,ATP含量和ATP5A基因表达显著增加(p < 0.05)。这些结果表明,在炎症条件下,细胞内C3激活可能通过C3a-C3aR信号和cAMP/PKA途径抑制牦牛瘤胃上皮细胞ATP的产生。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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