储存期间,含白细胞和非含白细胞的犬红细胞中丙二醛和游离血红蛋白的时间依赖性变化。

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Arianna Miglio, Aurora Barbetta, Valentina Cremonini, Olimpia Barbato, Giovanni Ricci, Valeria Toppi, Luca Avellini, Valentina Cavani, Maria Teresa Antognoni
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引用次数: 0

摘要

血液单位的储存决定了有害物质的积累,如丙二醛(MDA)和游离血红蛋白(fHb)。这可能导致一些并发症,包括受者的心血管、神经退行性和代谢紊乱。本研究的目的是评估犬白细胞(LR)和非白细胞(NLR)堆积红细胞(pRBC)在6周的储存期间的MDA和fHb浓度。采集了6只健康成年魏玛猎犬(3只雌性和3只雄性)的血液样本。全血保存在柠檬酸盐-磷酸-葡萄糖-腺苷-葡萄糖-甘露醇添加剂溶液(CPD-SAGM)袋中,每个供者生产2个pRBC单位(1个NLR和1个LR)并在4°C下保存42天。在第0、7、14、21、28、35和42天采集样品,使用犬类特异性ELISA法分析丙二醛(MDA),使用Harboe直接分光光度法分析游离血红蛋白(fHb)。结果显示,与NLR-pRBC血液单位相比,LR- prbc中MDA积累有统计学意义的减少,并且T6时LR中fHb值较低。然而,fHb水平没有明显差异。这些发现表明,白细胞减少可能限制血液储存过程中的氧化应激,减少与氧化损伤相关的输血的潜在不良影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Time-Dependent Changes in Malondialdehyde and Free-Hemoglobin in Leukoreduced and Non-Leukoreduced Canine Packed Red Blood Cells Units During Storage.

Storage of Blood units determines the accumulation of harmful substances, such as malondialdehyde (MDA) and free hemoglobin (fHb). These may lead to several complications, including cardiovascular, neurodegenerative, and metabolic disorders in recipients. The objective of this study was to evaluate the concentrations of MDA and fHb in canine leukoreduced (LR) and non-leukoreduced (NLR) packed red blood cells (pRBC) during the storage period of six weeks. Blood samples were collected from six healthy adult Weimaraner dogs (three females and three males). Whole blood was stored in citrate-phosphate-dextrose saline-adenine-glucose-mannitol additive solution (CPD-SAGM) bags and, for each donor, two pRBC units (one NLR and one LR) were produced and stored at 4 °C for 42 days. Samples were collected on days 0, 7, 14, 21, 28, 35, and 42, and analyzed for malondialdehyde (MDA) using a canine-specific ELISA method, and for free hemoglobin (fHb) using the Harboe direct spectrophotometric method. The results demonstrated a statistically significant reduction in MDA accumulation in LR-pRBC compared to NLR-pRBC blood units and lower values of fHb in LR at T6. However, no significant difference in fHb levels were demonstrated. These findings suggest that leukoreduction may limit oxidative stress during blood storage, reducing the potential adverse effects of transfusions related to oxidative damage.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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