Veterinary Sciences最新文献

{"title":"Administration of a Recombinant Fusion Protein of IFN-γ and CD154 Inhibited the Infection of Chicks with <i>Salmonella enterica</i>.","authors":"Jingya Zhang, Guofan Ren, Wei Li, Honglin Xie, Zengqi Yang, Juan Wang, Yefei Zhou, Xinglong Wang","doi":"10.3390/vetsci12020112","DOIUrl":"10.3390/vetsci12020112","url":null,"abstract":"<p><p>The cytokines IFN-γ and CD154 have been well established, and they play pivotal roles in immune protection against <i>Salmonella</i> in mice, but their effects and specific mechanisms in <i>Salmonella</i>-infected chickens are less understood. In this study, we conducted animal experiments to screen the highly immunoprotective chIFN-γ-chCD154 fusion protein compared with single protein chIFN-γ or chCD154 in white Leghorn chickens. The results showed that compared with separate pretreatments with chIFN-γ and chCD154, the fusion protein, chIFN-γ-chCD154, synergistically increased survival of infected chickens, reduced bacterial load in feces and organs, and attenuated pathological damage to the liver and cecum. Pretreatment with chIFN-γ-chCD154 also increased humoral immune responses, expression of the tight junction proteins zo-1, occludin, and claudin-1, and the relative abundance of <i>Enterococcus_cecorum</i>, <i>Lactobacillus_helveticus</i>, and <i>Lactobacillus_agilis</i>, which protect against intestinal inflammation. Compared with single protein pretreatment, chIFN-γ-chCD154 significantly upregulated STAT1, IRF1, and GBP1 in infected chickens while decreasing mRNA expression of TLR4, MyD88, NF-κB, TNF-α, IL-6, and IL-1β. In summary, damage to the cecal epithelial barrier and the inflammation induced by <i>S. typhimurium</i> infection was alleviated by chIFN-γ-chCD154 pretreatment through a mechanism involving the TLR4/MyD88/NF-κB and IFN-γ/STAT/IRF1/GBP1 pathways.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical Analysis of Inter-Alpha-Trypsin Inhibitor Heavy Chain 2 and Enolase 1 in Canine Mammary Tumors: Associations with Tumor Aggressiveness and Prognostic Significance.","authors":"Luadna Dos Santos E Silva, Pedro Henrique Fogaça Jordão, Beatriz Castilho Balieiro, Laura de Souza Baracioli, Daniela Farias de Nóbrega, Adriana Alonso Novais, Luiz Gustavo de Almeida Chuffa, Debora Aparecida Pires de Campos Zuccari","doi":"10.3390/vetsci12020110","DOIUrl":"10.3390/vetsci12020110","url":null,"abstract":"<p><p>Mammary neoplasms in dogs are a common clinical concern, especially in middle-aged and older intact females. These tumors share similarities with human breast cancer in terms of histology, disease progression, and risk factors, making dogs a relevant model for breast cancer research. The search for biomarkers in canine mammary tumors is essential to understand tumor progression and identify potential therapeutic targets. This study investigated the expression of two potential biomarkers-Inter-Alpha-Trypsin Inhibitor Heavy Chain 2 (ITIH2) and Enolase 1 (ENO1)-in the mammary glands of healthy and tumor-bearing dogs using immunohistochemistry. Both proteins were identified in previous proteomic analyses of extracellular vesicles derived from the plasma of healthy and tumor-bearing dogs. A total of fifty-one canine mammary tissue samples were analyzed and categorized into three groups: (i) the control group, composed of five samples of normal mammary tissue without neoplasia; (ii) benign tumors, composed of nineteen samples of benign mixed tumors; and (iii) malignant tumors, which included six carcinomas in grade 1 mixed tumors, five carcinomas in grade 2 mixed tumors, thirteen solid carcinomas of grade 3, one papillary carcinoma, and two tubular carcinomas. Regarding the intensity of staining, quantified by histoscore, there were no significant differences in the comparison between the groups; for ITIH2, the <i>p</i>-value was 0.33, and for ENO1, the <i>p</i>-value was 0.57. Regarding the predictive potential of their respective ROC curves, the proteins demonstrated low predictive power in canine mammary tumors. These findings indicate that neither ITIH2 nor ENO1 demonstrated strong prognostic value in this setting, as demonstrated by their moderate AUC values, wide confidence intervals, and lack of statistical significance. However, this study found distinct tissue localization patterns for ITIH2 and subcellular localization for ENO1. As an additional way to examine possible associations of these proteins with epithelial-mesenchymal transition, the ZEB1 antibody was tested by both single and double immunohistochemistry, demonstrating a tendency to be more intensely expressed in the malignant group and tending to be associated with ENO1 in canine mammary tumors.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paeoniflorin Inhibits Porcine Circovirus Type 2 Replication by Inhibiting Autophagy and Targeting AKT/mTOR Signaling.","authors":"Zhengchang Wu, Luchen Yu, Yueqing Hu, Wenbin Bao, Shenglong Wu","doi":"10.3390/vetsci12020117","DOIUrl":"10.3390/vetsci12020117","url":null,"abstract":"<p><p>Porcine circovirus type 2 (PCV2) is an important pathogen that leads to great economic losses to the swine industry. Paeoniflorin (PF), a novel plant extract, has been reported to have antiviral properties. However, the role of paeoniflorin in regulating PCV2 replication remains unclear. Here, we used the CCK8 assay to demonstrate that PF within safe concentrations (0-275 mM) significantly inhibits PCV2 replication in a dose-dependent manner in porcine kidney cells. Subsequently, comparative transcriptome and functional verification revealed that PF probably inherits PCV2 replication via targeting AKT/mTOR signaling. Further experimental data show that the AKT/mTOR signaling pathway is highly relevant to autophagy. Thus, experimental data from Western blot, qPCR, and the indirect immunofluorescence test indicate that PF inhibits PCV2 replication by inhibiting autophagy by targeting the AKT/mTOR signaling pathway. Together, our results provide insight into the mechanism of paeoniflorin in regulating PCV2 replication and offer new ideas for the treatment of PCV2 infection in pigs.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agreement and Diagnostic Accuracy of New Linear Deflection Oscillometry and Doppler Devices for Hypotension Detection Compared to Invasive Blood Pressure in Anesthetized Dogs.","authors":"Matheus M Mantovani, Any C A Costa, Mayara T de Lima, Luis F N Dos Santos, Kimberly F Silva, Alessandro R de C Martins, Adan W M Navarro, Renata S Akabane, Denise T Fantoni","doi":"10.3390/vetsci12020116","DOIUrl":"10.3390/vetsci12020116","url":null,"abstract":"<p><p>Blood pressure (BP) monitoring is essential during anesthesia to maintain cardiovascular stability and detect hypotension. This study evaluated the agreement and diagnostic accuracy of linear deflection oscillometry (LDO) and Doppler compared to invasive blood pressure (IBP) in anesthetized dogs. Eleven healthy dogs were anesthetized, and BP measurements were taken using LDO, Doppler, and IBP methods under normotensive and hypotensive conditions. The LDO device demonstrated superior agreement, assessed using Bland-Altman analysis, with IBP, especially in hypotensive conditions, compared to the Doppler method. LDO showed bias and standard deviation in the hypotensive state, with a mean and systolic arterial pressure (MAP and SAP) of -5.1 ± 7.9 and -5.6 ± 12.5 mmHg, respectively. Conversely, Doppler measurements tended to overestimate SAP during hypotension, presenting a bias of -13 ± 15.45 mmHg. The LDO achieved an area under the curve (AUC) of 0.809 for hypotension detection, with an MAP cutoff of ≤72 mmHg (sensitivity: 90%, specificity: 63%). Meanwhile, the best threshold for Doppler measurements was an AUC of 0.798, SAP ≤ 100 mmHg (sensitivity: 77.8%, specificity: 81.8%). These results indicate that LDO is a reliable method for hypotension detection in anesthetized dogs, with potential applications for real-time monitoring. In contrast, the Doppler method may help confirm hypotension diagnoses.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Perioperative Analgesic Efficacy of Lidocaine Intratesticular Block and Spermatic Cord Instillation in Feline Castration.","authors":"Tossawarn Makpunpol, Yanika Chanrobru, Passawan Thanapaisal, Manawee Kuropakaranan, Piyasak Wipoosak, Suvaluk Seesupa, Supranee Jitpean, Duangdaun Kaenkangploo, Preenun Jitasombuti, Chalermkwan Nonthakotr, Nitaya Boonbal, Somphong Hoisang, Wanwisa Chaoum, Naruepon Kampa, Thanikul Srithunyarat","doi":"10.3390/vetsci12020111","DOIUrl":"10.3390/vetsci12020111","url":null,"abstract":"<p><p>Intratesticular block is an effective local anesthetic technique for castration; however, its use may be limited in cases of testicular masses or infections. Instillation is a simple technique used in several procedures, though studies investigating its application in feline castration remain scarce. Therefore, this study aimed to compare the perioperative analgesic efficacy of lidocaine intratesticular block and spermatic cord instillation in feline castration. Forty-five male cats were randomly divided into three groups: the intratesticular block group (IT), the spermatic cord instillation group (S), and the control group (C). All cats were anesthetized using the same protocol and both techniques were performed based on group assignments. Lidocaine (2 mg/kg) was administrated according to the designated route, while 0.9% normal saline was used for the alternative route and in the C group. Intraoperative parameters were recorded, showing that noninvasive blood pressure in the IT and S groups was significantly lower than in the C group. Although both lidocaine intratesticular block and spermatic cord instillation effectively reduce surgical noxious stimulation, intratesticular block is recommended as it provides more stable intraoperative vital signs and less postoperative pain. Therefore, spermatic cord instillation can be considered as an optional technique for managing pain in feline castration.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of Tylvalosin Following Intravenous or Oral Administration at Different Doses in Broiler Chickens.","authors":"Zeyu Wen, Sumeng Chen, Jinyan Meng, Qinyao Wu, Runlin Yu, Nuoyu Xu, Jingyuan Kong, Lu Zhang, Xingyuan Cao","doi":"10.3390/vetsci12020118","DOIUrl":"10.3390/vetsci12020118","url":null,"abstract":"<p><p>Tylvalosin is a macrolide antimicrobial with antibacterial activity against Gram-positive bacteria, some Gram-negative organisms, and mycoplasma. It is used to treat respiratory and enteric bacterial infections in swine and poultry. In this study, we aimed to investigate the pharmacokinetic changes in tylvalosin following its intravenous or oral administration at doses of 5, 10, and 25 mg/kg in broiler chickens. Forty-eight broiler chickens were included in the study. The plasma concentrations of tylvalosin were measured by using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and its pharmacokinetic parameters were evaluated by using both non-compartmental and compartmental analyses. The linear mixed-effects model revealed no dose proportionality within the 5-25 mg/kg range for either administration route. Based on pharmacokinetic data from a single oral dose, this study simulated a multiple-dose regimen of tylvalosin (25 mg/kg), demonstrating that a 6-hour dosing interval reaches a steady state after the fourth dose. Furthermore, the results show that the absolute bioavailability of tylvalosin after oral administration in chickens was relatively low, with values of 5.92%, 3.56%, and 3.04% for the doses of 5, 10, and 25 mg/kg, respectively. Further studies are required to significantly improve the oral bioavailability of tylvalosin and similar compounds through formulation optimization.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tick Control Strategies: Critical Insights into Chemical, Biological, Physical, and Integrated Approaches for Effective Hard Tick Management.","authors":"Tsireledzo Goodwill Makwarela, Nimmi Seoraj-Pillai, Tshifhiwa Constance Nangammbi","doi":"10.3390/vetsci12020114","DOIUrl":"10.3390/vetsci12020114","url":null,"abstract":"<p><p>Ticks and tick-borne diseases significantly impact animal health, public health, and economic productivity globally, particularly in areas where the wildlife-livestock interface complicates management. This review critically examines the current control strategies, focusing on chemical, biological, physical, and integrated pest management (IPM) approaches. Chemical acaricides, while effective, are increasingly challenged by resistance development and environmental concerns. Biological approaches, including natural predators and entomopathogenic fungi, and physical interventions, such as habitat modification, provide sustainable alternatives but require further optimization. IPM stands out as the most promising long-term solution, integrating multiple approaches to enhance efficacy while reducing environmental risks. Emerging innovations, such as nanotechnology-enhanced acaricides and next-generation vaccines, offer promising avenues for improved tick control. Addressing the complex challenges of tick management requires tailored strategies, interdisciplinary collaboration, and sustained research investment in both veterinary and public health contexts.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Bagaza Virus in Europe: A Scoping Review.","authors":"Filipa Loureiro, João R Mesquita, Luís Cardoso, Ana C Matos, Manuela Matos, Ana Cláudia Coelho","doi":"10.3390/vetsci12020113","DOIUrl":"10.3390/vetsci12020113","url":null,"abstract":"<p><p>The Bagaza virus (BAGV) belongs to the genus <i>Orthoflavivirus</i> (Ntaya serocomplex) and emerged in Europe, Spain, in 2010. The natural transmission cycle of this virus is perpetuated by <i>Culex</i> spp. mosquitoes and viraemic birds. The ability of BAGV to cause infection in several game birds from the family Phasianidae has been well-studied. BAGV is antigenically similar to other orthoflaviviruses from the Japanese encephalitis serocomplex, such as the West Nile and Usutu viruses, a circumstance which can lead to cross-reactivity in less specific serological techniques (e.g., ELISA). Severe implications in animal health has already been described, but some aspects of the dynamics of transmission and the limits of zoonotic potential of BAGV still need to be clarified. Further investigation focused on epidemiological surveillance in high-risk areas would be beneficial for prevention and control of new outbreaks. The present study is a systematic review of the BAGV reports in Europe.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc Oxide Administration Relieves the Diarrhea of ETEC K88-Infected Piglets by Reducing Ileal Apoptosis and Maintaining Gut Microbial Balance.","authors":"Yanyan Zhang, Jiale Liu, Muzi Li, Yi Dong, Zongyun Li, Dan Yi, Tao Wu, Lei Wang, Di Zhao, Yongqing Hou","doi":"10.3390/vetsci12020115","DOIUrl":"10.3390/vetsci12020115","url":null,"abstract":"<p><p>The impact of ZnO as a feed additive on growth-performance and intestinal function of <i>Enterotoxigenic Escherichia coli</i> (ETEC) K88-infected piglets remains unclear. Fecal scores of piglets in ETEC group were significantly increased compared to control group. ETEC K88 significantly damages the small intestine, including a reduction in villus height in the jejunum, duodenum, and ileum, and a decrease in total superoxide dismutase activity in the jejunum and catalase activity in the ileum and jejunum. Compared to control group, ETEC K88 infection significantly elevated the mRNA level of gene <i>IL-1β</i> and the level of ileal epithelial cell apoptosis. ZnO administration significantly alleviated these negative effects and improved the antioxidative capability of the ileum. Moreover, ZnO supplementation alleviated the imbalance of gut microbiota by restoring the reduced amount of <i>Enterococcus</i> and <i>Lactobacillus</i> in the jejunum, <i>Clostridium</i> in the ileum, and <i>Lactobacillus</i> in the cecum, as well as the increased amount of total eubacteria in the ileum and <i>Enterococcus</i> in the cecum induced by the ETEC K88 infection. In conclusion, ZnO administration can reduce the diarrhea of piglets infected with ETEC K88 by reducing the structural damage of the intestine, attenuating intestinal oxidative stress and epithelial cell apoptosis, and modulating the gut microbiota.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Histochemical Characteristics of Submandibular Gland of the Bactrian Camel.","authors":"Yulu Chen, Guojuan Chen, Yumei Qi, Jianlin Zeng, Long Ma, Xudong Zhang, Xiaolong Qie, Yajuan Jin, Haijun Li, Ligang Yuan","doi":"10.3390/vetsci12020108","DOIUrl":"10.3390/vetsci12020108","url":null,"abstract":"<p><p>The ultrastructure of submandibular gland (SMG) of Bactrian camels was observed by a transmission electron microscope. Routine HE staining, special staining combined with immunohistochemistry, and immunofluorescence techniques were used to study the histochemical characteristics of the submandibular gland and the localisation and distribution characteristics of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR). HE results showed that the submandibular gland of Bactrian camels was composed of mixed serous and mucinous acini glands. The submandibular striated duct was highly developed and connected with intercalated ducts with larger diameter. Intercalated ducts are shorter and directly connected to acini. In AB-PAS staining, it was observed that the inner wall of striated tube was strongly positive for AB staining. The distribution of the reticular fibres around the follicles and ducts of the submandibular gland is distinct, with collagen fibres distributed mainly in the periphery of the ducts and sparse collagen fibres in the periphery of the acini. Immunohistochemistry and fluorescence show that EGF is strongly positive in striated and intercalated ducts, and EGFR is weakly positive in striated and intercalated ducts. Bactrian camel SMGs secrete more acidic mucins, and EGF and EGFR were mainly secreted and play a role in the pipeline system of SMGs.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}