Veterinary Sciences最新文献

{"title":"Etiological Detection, Isolation, and Pathogenicity of Porcine Reproductive and Respiratory Syndrome Virus in China.","authors":"Yingbin Du, Jingyi Chen, Tianze Ren, Chunying Xie, Yiye Zhang, Liurong Fang, Yanrong Zhou","doi":"10.3390/vetsci12060530","DOIUrl":"10.3390/vetsci12060530","url":null,"abstract":"<p><p>Due to its high genomic variability, the epidemiological landscape of porcine reproductive and respiratory syndrome virus (PRRSV) has become increasingly complex in recent years. From 2022 to 2023, we collected a total of 1044 clinical samples from pigs suspected of PRRSV infection in China and discovered a PRRSV-positive rate of 29.8% (311/1044) using RT-PCR targeting the nsp2 gene. Among these positive samples, NADC30/34-like PRRSV, highly pathogenic PRRSV (HP-PRRSV), and classical PRRSV strains accounted for 60.1%, 37.9%, and 4.5%, respectively. These results indicate that the most prevalent PRRSV strains in China are NADC30/34-like PRRSV, followed by HP-PRRSV. Two PRRSV strains, JX03 and HN08, were isolated, and TCID₅₀ assays were performed to determine their titers at different time points post-infection, revealing differences in their proliferation kinetics. Phylogenetic, amino acid sequence, and recombination analyses demonstrated that the JX03 and HN08 strains cluster within lineage 8 (HP-PRRSV) and sublineage 1.5 (NADC34-like PRRSV), respectively. Notably, the HN08 strain was identified as a recombinant between the NADC30-like and NADC34-like strains, while no recombination event was detected in the JX03 strain. Pathogenicity assessments showed that the JX03 strain exhibited higher pathogenicity than the CHN-HB-2018 strain (a NADC30-like PRRSV strain was previously isolated by our lab), as evidenced by differences in clinical signs and mortality rates in piglets. In contrast, HN08 displayed no obvious clinical symptoms or mortality, revealing lower pathogenicity than the CHN-HB-2018 strain. These findings provide valuable information on the epidemiological and genetic characteristics of PRRSV strains in China, laying a foundation for the development of effective strategies against PRRSV.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Non-Tuberculous Mycobacteria in Iberian Lynx (<i>Lynx pardinus</i>) and Their Impact on Its Health.","authors":"Natalia Jiménez-Pizarro, Beatriz Serrano, Jorge Peña, Rafael Barrera, María Gil-Molino, David Risco, Javier Hermoso-de-Mendoza","doi":"10.3390/vetsci12060527","DOIUrl":"10.3390/vetsci12060527","url":null,"abstract":"<p><p>The Iberian lynx (<i>Lynx pardinus</i>) population has been increasing in recent years thanks to the collaboration of several associations within the framework of the Life Lynxconnect project, which promotes captive breeding for the subsequent release of specimens into the environment. It is therefore important to know their population status, the diseases to which these animals are exposed, and how they affect their repopulation. In this sense, this study aims to study how the presence of mycobacteria affects the lynx population in Extremadura (southwest of Spain). To this end, blood samples, tracheal swabs, and tracheobronchial washings from live-captured lynxes, as well as mediastinal and mesenteric lymph nodes from roadkill animals, were collected. The association between the presence of mycobacteria and various factors-including body condition, age, sex, and blood parameters-was subsequently evaluated. Up to date, our findings show the absence of tuberculosis (TB) in all lynxes tested, while they seem to be reservoirs of non-tuberculous mycobacteria (NTM), mainly <i>M. lentiflavum</i>, the most isolated species. Nonetheless, these mycobacteria appear to have no significant effect on the health condition of the animals.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Removal of Plasma Proteins by Double-Filtration Plasmapheresis in Canine Blood: An Ex Vivo Study and Retrospective Report of In Vivo Clinical Treatments in Three Dogs.","authors":"Roberta Troia, Claudia Iannucci, Lisa Niemann, Alessio Vigani","doi":"10.3390/vetsci12060528","DOIUrl":"10.3390/vetsci12060528","url":null,"abstract":"<p><p>Double-filtration plasmapheresis (DFPP) is a plasma-exchange modality that allows selective clearance of high-molecular-weight proteins, potentially minimizing albumin loss and the need for volume replacement. Reports concerning DFPP use in dogs are scarce. This study evaluates the quantitative net loss of different plasma proteins fractions in an ex vivo model using canine blood processed via DFPP. DFPP treatment with INUSpheresis^® processing 1.5, 2, and 3 plasma volumes (PVs) was performed. Plasma proteins fractions were measured in the reservoir blood bag at baseline (pre-treatment) and in the effluent bag at the end of each target PV exchanged to calculate the net loss of selected plasma proteins. At 1.5 PV, net globulin and albumin loss was 41 and 25% respectively. At 3 PV, net globulin and albumin loss was 47 and 40%, respectively. Fibrinogen concentration was unmeasurable low in the reservoir blood bag after processing 1.5 PV. INUSpheresis^® allows selective plasma proteins removal, with a sparing effect on albumin at 1.5 PV. Selectivity is, however, progressively reduced with incremental target PV. A description of five DFPP treatments in three dogs is additionally presented. Semi-selective protein removal was also demonstrated in vivo, with a significantly lower percentage reduction in albumin compared to total globulin (<i>p</i> = 0.01) and fibrinogen (<i>p</i> = 0.007).</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Correlation Between Aggressive Behavior and Gut Microbiota and Serum Serotonin (5-HT) in Working Dogs.","authors":"Ning Sun, Liuwei Xie, Jingjing Chao, Fuxiao Xiu, He Zhai, Yuanting Zhou, Xi Yu, Yingyi Shui","doi":"10.3390/vetsci12060526","DOIUrl":"10.3390/vetsci12060526","url":null,"abstract":"<p><p>Aggressive canine behavior poses a significant threat to public health. Understanding aggressive behavior is crucial for canine socialization and human-dog interactions. This study conducted an exploratory analysis of working dogs to investigate changes in gut microbiota and neurotransmitters associated with aggressive behavior. Notably, it represents the first research to systematically differentiate canine aggression into offensive and defensive subtypes for investigation. In this study, 56 working dogs from three regions of China, comprising different breeds (11 Spaniels, 13 German Shepherds, and 32 Belgian Malinois), aged 4.89 ± 1.54 years, and of both sexes (38 males and 18 females), were assessed and grouped for aggressive behavior using a C-BARQ-based questionnaire. Then, 16S rRNA sequencing and ELISA were employed to compare differences in gut microbiota and serotonin concentrations between aggressive (n = 35) and non-aggressive (n = 21) groups, as well as between offensive (n = 26) and defensive (n = 9) aggression subgroups. β-diversity analysis confirmed no significant correlation between aggressive behavior and gut microbiota composition (<i>p</i> > 0.05), suggesting a limited role of gut microbiota in modulating host behavior. Comparative analysis of gut microbiota composition revealed no significant differences in phylum-level abundance among different aggression types (<i>p</i> > 0.05). Notably, the non-aggressive group exhibited significantly higher relative abundances of <i>Escherichia-Shigella</i>, <i>Erysipelotrichaceae_UCG-003</i>, and <i>Clostridium_sensu_stricto_1</i> compared to the aggressive group (<i>p</i> < 0.05). Random forest analysis identified <i>Lactobacillus</i> as a biomarker for canine aggressive behavior and <i>Turicibacter</i> as a discriminatory factor between offensive and defensive aggression. The results demonstrated a strong correlation between aggression and 5-HT neurotransmission. Serum serotonin levels were significantly lower in both the defensive (39.92 ± 2.58 ng/mL) and offensive (50.07 ± 3.90 ng/mL) aggression groups compared to the non-aggressive group (59.49 ± 2.76 ng/mL), with the lowest levels found in defensively aggressive dogs. The defensive group showed significantly lower serotonin levels than the offensive group (<i>p</i> < 0.001). The results demonstrate that different behavioral phenotypes in aggressive dogs lead to distinct gut microbiome compositions. This suggests that microbiome analysis may facilitate early diagnosis and preventive intervention before aggressive behavior manifests. As such, 5-HT shows potential as a monitoring tool for diagnosing canine aggression, with significant practical applications in canine behavior management.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-Volume (0.3 mL/kg) Ropivacaine 0.5% for a Quadratus Lumborum Block in Cats Undergoing Ovariectomy: A Randomized Study.","authors":"Chiara Di Franco, Camilla Cozzani, Iacopo Vannozzi, Angela Briganti","doi":"10.3390/vetsci12060524","DOIUrl":"10.3390/vetsci12060524","url":null,"abstract":"<p><p>Elective surgical ovariectomy is one of the most common surgical procedures in veterinary medicine [...].</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rumen-Protected Fat and Rumen-Protected Choline Co-Supplementation: Impacts on Performance and Meat Quality of Growing Lambs.","authors":"Haitao Liu, Fadi Li, Fei Li, Zhiyuan Ma, Tao Wang, Qinwu Li, Xinji Wang, Kaidong Li","doi":"10.3390/vetsci12060525","DOIUrl":"10.3390/vetsci12060525","url":null,"abstract":"<p><p>This study aimed to assess the effect of co-supplementing rumen-protected fat and rumen-protected choline on growth performance, carcass traits, and meat quality in lambs. Using a randomized experimental design, 45 weaned female Tian×Hu crossbred lambs (3 months old; average body weight: 27.34 ± 0.57 kg, mean ± SD) were randomly allocated to one of three dietary treatment groups. The three dietary treatments were as follows: a basal diet group (CON), a group receiving 2% rumen-protected fat in place of 2% barley (RPF), and a group supplemented with 2% rumen-protected fat and 0.4% rumen-protected choline, replacing 2% barley and 0.4% corn germ in the basal diet (RPFC). Compared to the CON group, neither the RPF nor RPFC treatments resulted in significant differences in growth performance (<i>p</i> > 0.05). However, the RPFC group showed a 5.3% increase in dry matter intake (DMI) compared to the RPF group (<i>p</i> < 0.05). Compared with the CON, the RPF treatment increased 69.23% the relative abundance of C18:2n-6t (<i>p</i> < 0.05), but the content of C17:0, C17:1, C18:1n-9c, and iso-C18:0 in <i>Longissimus lumborum</i> was decreased by 16.49%, 15.78%, 6.45% and 27.78%, respectively (<i>p</i> < 0.05). The RPFC treatment increased the relative abundance of C16:1 in <i>Longissimus lumborum</i> (<i>p</i> < 0.05). The RPF and RPFC treatments significantly increased serum levels of high-density lipoprotein (HDL) and total cholesterol compared to the CON group (<i>p</i> < 0.05). The RPF treatment raised HDL by 50.00% and total cholesterol by 38.03%, while the RPFC treatment increased HDL by 39.47% and total cholesterol by 26.03%. Furthermore, compared to the RPF group, the RPFC treatment led to a 13.47% increase in the 45 min b* color value of the <i>Longissimus lumborum</i> (<i>p</i> < 0.01) and a significant 45.45% reduction in the relative abundance of C18:2n-6t fatty acid in the same muscle (<i>p</i> < 0.05). In summary, rumen-protected choline reduces the negative effects of rumen-protected fat on feed intake in lambs and changes fatty acid profile in meat.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infrared Thermography and Physiological Variables as Methods for Recognizing Fear in Domestic Cats (<i>Felis catus</i>) Using Three Pharmacological Models: Cannabidiol, Gabapentin, and Synthetic Facial Pheromones.","authors":"Fabiola Torres-Bernal, Julio Martínez-Burnes, Ismael Hernández-Avalos, Adriana Olmos-Hernández, Adriana Domínguez-OIiva, Brenda Reyes-Sotelo, Cynthia González-López, Diana Villanueva-Pereyra, Daniel Mota-Rojas","doi":"10.3390/vetsci12060523","DOIUrl":"10.3390/vetsci12060523","url":null,"abstract":"<p><p>This study aimed to assess changes in the surface temperature and physiological parameters of cats exposed to a fear model involving negative dog-cat interactions, receiving three pharmacological options: a single dose of cannabidiol, gabapentin, or synthetic facial pheromones. The surface temperature of the upper and lower limbs, facial, dorsal, and appendicular thermal windows was assessed through infrared thermography. Additionally, heart rate, respiratory rate, and rectal temperature were recorded. Eighty male and female domestic cats were included in the study and randomly divided into four groups: CONTROL (placebo, 2 mL/cat orally), CBD (cannabidiol, 2 mg/kg orally), GABA (gabapentin, 100 mg/cat orally), and SFP (synthetic facial pheromone, two sprays/carrier). All cats underwent six experimental phases: T_basal-, T1st_fear, T1st_recovery, T_basal+, T2nd_fear, and T2nd_recovery. Drug administration was carried out at the end of T1st_recovery; the time between drug administration and T_basal+ differed according to each drug's nature. Statistical differences were obtained between experimental groups and times in the average surface temperature of thermal windows and cardiorespiratory parameters. In particular, the CBD, GABA, and SFP groups exhibited differences during T2nd_fear, in contrast to the control and T1st_fear groups. In conclusion, the results suggest a pharmacological effect of CBD, GABA, and SFP on cats' physiological alterations in response to fear.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Nanoemulsions of Betulinic Acid on the Development of Canine Mammary Tumors.","authors":"Zayra Yeretzi Amoros-Cerón, Juan Manuel Pinos-Rodríguez, Hugo Sergio García, Angélica Olivares-Muñoz, Isaac De Gasperin-López, Argel Flores-Primo","doi":"10.3390/vetsci12060522","DOIUrl":"10.3390/vetsci12060522","url":null,"abstract":"<p><p>Mammary gland tumors in dogs are very common in clinical practice. Betulinic acid is currently a compound considered to have anticancer properties in human mammary tumors via nanoemulsions. In this study, betulinic acid nanoemulsions with a particle size of less than 300 nm were prepared. Biopsies were obtained from five female dogs with mammary tumors for histopathological analysis, confirming that two were tubular mammary carcinomas (MMTs, malignant) and three were complex mammary adenomas (BMTs, benign). The five female dogs were administered with a daily oral dose of nanoemulsion containing 5 mg/kg of betulinic acid for 30 days. Tumor size was measured every 7 days, and the response to treatment was assessed according to RECIST (Response Evaluation Criteria In Solid Tumors) standards. In one of the females with MMTs treated with the nanoemulsion, the tumor size was reduced by approximately 38%, while in the BMT female dogs, the nanoemulsion reduced the tumor size by 25.3%. It was concluded that oral administration of betulinic acid nanoemulsions reduced the size of canine mammary tumors. Experimental studies are still needed to further evaluate this preparation.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Efficacy of Two Mycotoxin Detoxifications on Laying Performance, Antioxidant Capacity, and Liver Damage of Laying Hens Fed Diet Naturally Contaminated with Low-Level Mycotoxins.","authors":"Huimin Ma, Wentao Cheng, Usman Nazir, Chengfei Wang, Haiming Yang, Xiaoli Wan","doi":"10.3390/vetsci12060520","DOIUrl":"10.3390/vetsci12060520","url":null,"abstract":"<p><p>This study was conducted to investigate the effects of two mycotoxin detoxifications on laying hens. A total of 360 70-week-old Hy-Line Brown laying hens were randomly divided into 1 of the 4 dietary treatment groups, with 6 replicates per group and 15 hens per replicate. The laying hens of the four treatments were fed with a basal diet (CON), a diet with naturally low-level mycotoxin contaminated corn (the levels of AFB₁, ZEA, and DON in the corn of the CON group were 1.68 μg/kg, 42.75 μg/kg, and 585.40 μg/kg, respectively), replacing 73% of the corn in CON (MC), the MC diet with 1 g/kg modified silica-aluminate mycotoxin adsorbent (MA), and the MC diet with 1 g/kg mycotoxin degrading enzyme and bacteria complexes degradation agent (MD), respectively. Liver tissue and serum samples were extracted at the end of the trial to assess the antioxidant status and hepatic injury biomarkers. The experimental data were preliminarily interpreted in Excel and then analyzed by one-way ANOVA using SPSS, version 25 (SPSS Inc., Chicago, IL, USA). The results showed that compared to the CON, the MC group had decreased laying rate (<i>p</i> < 0.05), ferric reducing antioxidant potential in serum (<i>p</i> < 0.05), and reduced glutathione (GSH) content in the liver, and increased feed/egg ratio, serum alanine aminotransferase (ALT) activity, and malondialdehyde (MDA) content. Compared to the MC group, feed/egg ratio and serum ALT activity in the MA group and MD group decreased (<i>p</i> < 0.05), while serum GSH content and superoxide dismutase activity in the liver of the MA and MD groups increased. The liver glutathione peroxidase activity and the egg yolk percentage in the MA group decreased (<i>p</i> < 0.05) compared to the MC group. Histopathological alterations in liver tissues induced by mycotoxin included vacuolar degeneration, hepatocyte necrosis and disintegration, inflammatory cell infiltration, and enlarged hepatic sinuses. In short, both MA and MD were effective in mitigating the combined effects of low-level mycotoxins on laying hen performance, antioxidant capacity and liver damage.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, Histological, and Immunohistochemical Insights into a Canine Hepatic Myofibroblastic Sarcoma.","authors":"Valentina Rinaldi, Laura Nordio, Massimo Vignoli, Stefano Masci, Lorenzo Ressel, Paolo Emidio Crisi","doi":"10.3390/vetsci12060521","DOIUrl":"10.3390/vetsci12060521","url":null,"abstract":"<p><p>Hepatic sarcomas are rare and aggressive tumors in veterinary medicine, with limited reports in the literature. This case report describes a canine hepatic myofibroblastic sarcoma in a 5-year-old spayed female Dobermann. The dog presented with abdominal enlargement and was diagnosed with a large hepatic mass following comprehensive diagnostic evaluations, including blood tests, imaging, and histopathology. Histological and immunohistochemical analyses confirmed the tumor's myofibroblastic origin, characterized by positivity for markers such as vimentin, glial fibrillary acidic protein (GFAP), nerve growth factor receptor (NGFR), alpha-smooth muscle actin (SMA), and muscular actin (HHF35). Treatment involved a combination of intense-dose chemotherapy using doxorubicin and a subsequent metronomic chemotherapy protocol, which resulted in prolonged survival of over 690 days at the time this manuscript was written. This case highlights the importance of extensive diagnostic and immunohistochemical profiling in the accurate classification of and treatment planning for hepatic sarcomas, and emphasizes the role of advanced veterinary diagnostics in improving patient outcomes. Future studies with larger sample sizes are needed to enhance understanding of the biological behavior and optimal therapeutic strategies for such rare tumors.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12197649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}