中国猪繁殖与呼吸综合征病毒的病原学检测、分离和致病性。

IF 2 2区 农林科学 Q2 VETERINARY SCIENCES
Yingbin Du, Jingyi Chen, Tianze Ren, Chunying Xie, Yiye Zhang, Liurong Fang, Yanrong Zhou
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引用次数: 0

摘要

猪繁殖与呼吸综合征病毒(PRRSV)由于其高基因组变异性,近年来其流行病学格局变得越来越复杂。从2022年到2023年,我们在中国共收集了1044份疑似PRRSV感染猪的临床样本,采用靶向nsp2基因的RT-PCR方法发现PRRSV阳性率为29.8%(311/1044)。其中,nadc30 /34样PRRSV、高致病性PRRSV (HP-PRRSV)和经典PRRSV分别占60.1%、37.9%和4.5%。结果表明,中国流行的PRRSV毒株为nadc30 /34样PRRSV,其次为HP-PRRSV。分离两株PRRSV JX03和HN08,采用TCID50检测感染后不同时间点的滴度,揭示其增殖动力学的差异。系统发育、氨基酸序列和重组分析表明,JX03和HN08菌株分别聚集在谱系8 (HP-PRRSV)和亚谱系1.5 (nadc34样PRRSV)。值得注意的是,HN08菌株是nadc30样和nadc34样菌株之间的重组菌株,而JX03菌株未检测到重组事件。致病性评估显示,JX03株的致病性高于CHN-HB-2018株(本实验室此前分离到一株类似nadc30的PRRSV株),这一点在仔猪的临床症状和死亡率方面存在差异。HN08无明显临床症状和病死率,致病性低于CHN-HB-2018。这些发现为了解中国PRRSV流行病学和遗传特征提供了有价值的信息,为制定有效的PRRSV防治策略奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Etiological Detection, Isolation, and Pathogenicity of Porcine Reproductive and Respiratory Syndrome Virus in China.

Due to its high genomic variability, the epidemiological landscape of porcine reproductive and respiratory syndrome virus (PRRSV) has become increasingly complex in recent years. From 2022 to 2023, we collected a total of 1044 clinical samples from pigs suspected of PRRSV infection in China and discovered a PRRSV-positive rate of 29.8% (311/1044) using RT-PCR targeting the nsp2 gene. Among these positive samples, NADC30/34-like PRRSV, highly pathogenic PRRSV (HP-PRRSV), and classical PRRSV strains accounted for 60.1%, 37.9%, and 4.5%, respectively. These results indicate that the most prevalent PRRSV strains in China are NADC30/34-like PRRSV, followed by HP-PRRSV. Two PRRSV strains, JX03 and HN08, were isolated, and TCID50 assays were performed to determine their titers at different time points post-infection, revealing differences in their proliferation kinetics. Phylogenetic, amino acid sequence, and recombination analyses demonstrated that the JX03 and HN08 strains cluster within lineage 8 (HP-PRRSV) and sublineage 1.5 (NADC34-like PRRSV), respectively. Notably, the HN08 strain was identified as a recombinant between the NADC30-like and NADC34-like strains, while no recombination event was detected in the JX03 strain. Pathogenicity assessments showed that the JX03 strain exhibited higher pathogenicity than the CHN-HB-2018 strain (a NADC30-like PRRSV strain was previously isolated by our lab), as evidenced by differences in clinical signs and mortality rates in piglets. In contrast, HN08 displayed no obvious clinical symptoms or mortality, revealing lower pathogenicity than the CHN-HB-2018 strain. These findings provide valuable information on the epidemiological and genetic characteristics of PRRSV strains in China, laying a foundation for the development of effective strategies against PRRSV.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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