Proinflammatory Cytokines, Type I Interferons, and Specialized Proresolving Mediators Hallmark the Influence of Vaccination and Marketing on Backgrounded Beef Cattle.

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Hudson R McAllister, Sarah F Capik, Kelsey M Harvey, Bradly I Ramirez, Robert J Valeris-Chacin, Amelia R Woolums, Brandi B Karisch, Paul S Morley, Matthew A Scott
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Abstract

Cattle marketed through auction market systems and/or that remain unvaccinated are considered higher risk for BRD, but impacts on host response remain unclear. We sought to identify specific genomic patterns of beef calves vaccinated against BRD viruses or not and commercially marketed or directly transported in a split-plot randomized controlled trial. Forty-one calves who remained clinically healthy from birth through backgrounding were selected (randomly stratified) from a larger cohort of cattle (n = 81). Treatment groups included VAX/DIRECT (n = 12), VAX/AUCTION (n = 11), NOVAX/DIRECT (n = 7), and NOVAX/AUCTION (n = 11). Blood RNA was acquired across five time points, sequenced, and bioinformatically processed via HISAT2 and StringTie2. Significant transcriptional changes (FDR < 0.05) were observed at backgrounding entry (T5) in NOVAX/AUCTION cattle exhibiting 2809 uniquely differentially expressed genes and relative activation of immune, inflammatory, and metabolic pathways with upregulation of interferon-stimulated genes (e.g., IFIT3, MX2, and TRIM25) and downregulation of specialized proresolving mediator (SPM) enzymes (ALOX5 and ALOX15). VAX/AUCTION cattle exhibited modulated immune activation and preserved expression of SPM-associated genes when compared to NOVAX/AUCTION cattle. Both marketing route and vaccination shape the molecular immune landscape during high-stress transitions, with preweaning vaccination potentially modulating this response. This study provides mechanistic insight into how management practices influence immunological resilience and highlights the value of integrating transcriptomics into BRD risk mitigation.

促炎细胞因子,I型干扰素和专门的促炎介质标志着疫苗接种和营销对背景肉牛的影响。
通过拍卖市场系统销售和/或未接种疫苗的牛被认为有较高的BRD风险,但对宿主反应的影响尚不清楚。我们试图在一项分割区随机对照试验中确定接种BRD病毒疫苗或未接种BRD病毒疫苗、商业销售或直接运输的牛肉犊牛的特定基因组模式。从一个较大的牛群(n = 81)中(随机分层)选择了41头从出生开始就临床健康的小牛。治疗组包括VAX/DIRECT (n = 12)、VAX/AUCTION (n = 11)、NOVAX/DIRECT (n = 7)和NOVAX/AUCTION (n = 11)。通过5个时间点采集血液RNA,进行测序,并通过HISAT2和StringTie2进行生物信息处理。在NOVAX/AUCTION牛的背景进入(T5)时,观察到显著的转录变化(FDR < 0.05),显示2809个独特差异表达基因,干扰素刺激基因(如IFIT3、MX2和TRIM25)上调,特殊促生介质(SPM)酶(ALOX5和ALOX15)下调,免疫、炎症和代谢途径相对激活。与NOVAX/AUCTION牛相比,VAX/AUCTION牛表现出调节的免疫激活和保留的spm相关基因表达。在高应激过渡期间,营销途径和疫苗接种都会形成分子免疫格局,断奶前接种疫苗可能会调节这种反应。这项研究为管理实践如何影响免疫弹性提供了机制见解,并强调了将转录组学整合到BRD风险缓解中的价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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