RNA Sequencing-Based Transcriptome Analysis of Liver in Laying Hens Supplemented with Dietary Probiotic Bacillus Species and Prebiotic Yeast (Saccharomyces cerevisiae) Cell Walls.

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Ala E Abudabos, Zafar M Hakami, Ali R Al Sulaiman, Riyadh S Aljumaah, Valentino Palombo, Mashael R Aljumaah, Mariasilvia D'Andrea, Abdulrahman S Alharthi, Rashed A Alhotan
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引用次数: 0

Abstract

To investigate the impacts of dietary Bacillus-based probiotics and yeast-derived prebiotics on the hepatic transcriptome profile, 500 Hisex White laying hens were randomly allotted into five dietary treatments from 37 to 52 weeks of age: control; control + Bacillus subtilis; control + Bacillus subtilis and Bacillus licheniformis; control + Bacillus coagulans; and control + Saccharomyces cerevisiae yeast cell wall. Transcriptome analysis revealed a substantial number of differentially expressed genes exclusively between the control and prebiotic groups, identifying 2221 genes (FDR ≤ 0.05), with 980 genes upregulated (log2 fold change 0.69 to 24.62) and 1241 downregulated (log2 fold change -0.74 to -26.46). The top 10 upregulated KEGG pathways included protein export, glycerophospholipid metabolism, tryptophan metabolism, amino acid biosynthesis, alanine, aspartate, and glutamate metabolism, cofactor biosynthesis, propanoate metabolism, ABC transporters, 2-oxocarboxylic acid metabolism, and protein processing within the endoplasmic reticulum. In contrast, the most prominently downregulated pathways encompassed fructose and mannose metabolism, hedgehog signaling, PPAR signaling, Notch signaling, GnRH signaling, cell adhesion molecules, cytokine-cytokine receptor interactions, apelin signaling, glycosaminoglycan degradation, and RIG-I-like receptor signaling. These findings advance understanding of the hepatic transcriptomic response to yeast-derived prebiotics and identify key molecular pathways that could be targeted to enhance metabolic function in laying hens.

基于RNA测序的饲粮添加益生菌芽孢杆菌和益生元酵母细胞壁对蛋鸡肝脏转录组的影响
为研究饲粮中添加芽孢杆菌益生菌和酵母源益生菌对肝脏转录组谱的影响,选取500只Hisex白蛋鸡,从37 ~ 52周龄随机分为5组:对照组;对照+枯草芽孢杆菌;对照+枯草芽孢杆菌和地衣芽孢杆菌;对照+凝固芽孢杆菌;对照+酿酒酵母酵母细胞壁。转录组分析显示,对照组和益生元组之间存在大量差异表达基因,共鉴定出2221个基因(FDR≤0.05),其中980个基因上调(log2倍变化0.69 ~ 24.62),1241个基因下调(log2倍变化-0.74 ~ -26.46)。前10位上调的KEGG通路包括蛋白质输出、甘油磷脂代谢、色氨酸代谢、氨基酸生物合成、丙氨酸、天冬氨酸和谷氨酸代谢、辅助因子生物合成、丙酸代谢、ABC转运蛋白、2-氧羧酸代谢和内质网内的蛋白质加工。相比之下,最显著的下调途径包括果糖和甘露糖代谢、hedgehog信号、PPAR信号、Notch信号、GnRH信号、细胞粘附分子、细胞因子-细胞因子受体相互作用、apelin信号、糖胺聚糖降解和rig - i样受体信号。这些发现促进了对酵母菌衍生益生元的肝脏转录组反应的理解,并确定了可能增强蛋鸡代谢功能的关键分子途径。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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