泰国宋卡斑鸠排泄物中新型隐球菌的基因组特征

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Saowakon Indoung, Sanicha Chumtong, Sakaoporn Prachantasena, Ratchakul Wiriyaprom, Komwit Surachat, Sarunyou Chusri, Rattanaruji Pomwised, Ruttayaporn Ngasaman
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引用次数: 0

摘要

本研究旨在研究从鸟类排泄物中分离的新型隐球菌的基因组。13株分离株分别用ITS1-ITS4和CN4-CN5引物进行巢式聚合酶链反应进行生化检测。抗真菌药敏试验及全基因组测序分析。结果表明,所有分离株对两性霉素B(100%)、氟康唑和伊曲康唑(92.3%)敏感。其中一株(DOP3)对氟康唑和伊曲康唑(MIC分别为64µg/mL和8µg/mL)耐药。系统发育树显示其为新型C.(血清型A)。抗性菌株DOP3和非抗性菌株DOP3.1的基因组有14条染色体。DOP3由38个候选抗真菌抗性基因组成,这些基因对唑类药物最具活性(14)。唑组的注释基因大多属于atp结合盒转运体跨膜超家族。耐药基因主要存在于转录因子(HAP2、HAP5)、锌指(NRG1)、细胞色素P450 (ERG11)和myb样dna结合域(REB1)中。最常见的耐药基因是细胞色素P450 (ERG5和ERG11)和转录因子(HAP5)。DOP3.1还包含26个抗唑类药物的候选抗性基因。抗唑类基因属于ABC转运蛋白跨膜超家族。抗FCZ的抗性基因为细胞色素P450 (ERG11)、锌指(NRG1)和CCAAT结合转录因子(HAP2)。发现了细胞色素P450 (ERG5)对ITZ的抗性基因。本研究首次报道了斑鸠排泄物中新形态隐球菌(血清型A)的发现、对耐药菌株的药敏以及耐药基因的鉴定。应严格实施养殖场卫生,免疫能力强的人应避免接触斑鸠排泄物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genomic Characterization of Cryptococcus neoformans Isolated from Zebra Dove Excreta in Songkhla, Thailand.

The research aimed to study the genome of Cryptococcus neoformans isolated from bird excreta. Thirteen isolates were cultured, colony stained, and underwent biochemical testing confirmation by nested polymerase chain reaction using ITS1-ITS4 and CN4-CN5 primers, respectively. Antifungal susceptibility testing and whole-genomic sequencing were analyzed. The results determined that all isolates were susceptible to amphotericin B (100%), fluconazole, and itraconazole (92.3%). One isolate (DOP3) showed resistance to fluconazole and itraconazole (MIC >64 and >8 µg/mL, respectively). A phylogenetic tree showed the identity of C. neoformans (serotype A). The genome of resistant (DOP3) and non-resistant isolates (DOP3.1) had 14 chromosomes. DOP3 consisted of 38 candidate antifungal resistance genes, which were the most active against azoles (14). The annotated genes in the azole group mostly were in the ATP-binding cassette transporter transmembrane superfamily. Resistance genes against FCZ were in the transcription factors (HAP2, HAP5), zinc finger (NRG1), cytochrome P450 (ERG11), and Myb-like DNA-binding domain (REB1). The most frequent resistance genes against ITZ were cytochrome P450 (ERG5 and ERG11) and a transcription factor (HAP5). DOP3.1 also consisted of 26 candidate resistance genes against azoles. Resistance genes against the azole group belong to the ABC transporter transmembrane superfamily. Resistance genes against FCZ belong to cytochrome P450 (ERG11), the zinc finger (NRG1), and the CCAAT binding transcription factor (HAP2). Resistance genes belonging to cytochrome P450 (ERG5) were found against ITZ. This research provides the first report of C. neoformans (serotype A) in zebra dove excreta, drug susceptibility to a resistant strain, and identification of resistance genes. Farm sanitation should be strictly applied, and immunocompetent people should avoid contact with zebra dove excreta.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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