A Longitudinal Observational Study on Lactation-Associated Changes in Procalcitonin, Protein Carbonyl Content, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Dairy Cattle.

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Giulia Sala, Matteo Castelli, Chiara Orsetti, Giovanni Armenia, Lucia De Marchi, Valentina Meucci, Micaela Sgorbini, Francesca Bonelli
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引用次数: 0

Abstract

Procalcitonin (PCT), protein carbonyl content (PCC), asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA) have been proposed as promising biomarkers for detecting diseases in cattle. Their concentrations could potentially be influenced by lactation due to oxidative stress commonly observed during this period. This study aimed to evaluate plasma levels of PCT, PCC, ADMA, and SDMA at different stages of lactation in 21 healthy dairy cows: at 15 (T0), 60 (T1), and 150 (T2) days in milk (DIM). Clinically healthy Italian Holstein-Friesian cows were included, selected based on healthy dry periods and weekly veterinary checks during lactation. Blood samples were collected at each time point and biomarkers were measured using validated analytical methods. Data were analyzed using Friedman's test and the p value was set at 0.05. Median (IQR) PCT values were 64.29 (40.00-143.23), 75.36 (40.00-161.47), and 77.50 pg/mL (40.00-120.18) at T0, T1, and T2, respectively. PCC medians were 0.17 (0.10-0.27), 0.14 (0.08-0.23), and 0.20 (0.08-0.22) nmol/mL/mg; ADMA values were 0.11 (0.09-0.15), 0.11 (0.09-0.13), and 0.10 µmol/L (0.09-0.14); and SDMA values were 0.11 (0.09-0.14), 0.12 (0.09-0.15), and 0.10 µmol/L (0.09-0.16). No statistically significant differences were observed between time points for any biomarker. These findings suggest that, despite physiological oxidative stress during lactation, these biomarkers remain stable in healthy cows. Therefore, establishing distinct reference ranges based on lactation stage may not be necessary.

奶牛降钙素原、蛋白羰基含量、不对称二甲基精氨酸和对称二甲基精氨酸与泌乳期相关变化的纵向观察研究
原降钙素(PCT)、蛋白羰基含量(PCC)、不对称二甲基精氨酸(ADMA)和对称二甲基精氨酸(SDMA)已被提出作为检测牛疾病的有前途的生物标志物。它们的浓度可能受到哺乳期氧化应激的潜在影响。本研究旨在评价21头健康奶牛泌乳不同阶段(15 (T0)、60 (T1)和150 (T2) d)血浆PCT、PCC、ADMA和SDMA的水平。临床健康的意大利荷尔斯坦-弗里西亚奶牛包括在内,根据健康的干燥期和哺乳期间的每周兽医检查选择。在每个时间点采集血液样本,并使用经过验证的分析方法测量生物标志物。数据分析采用Friedman’s检验,p值设为0.05。T0、T1和T2的中位(IQR) PCT值分别为64.29(40.00-143.23)、75.36(40.00-161.47)和77.50 pg/mL(40.00-120.18)。PCC中位数分别为0.17(0.10-0.27)、0.14(0.08-0.23)和0.20 (0.08-0.22)nmol/mL/mg;ADMA值分别为0.11(0.09 ~ 0.15)、0.11(0.09 ~ 0.13)、0.10µmol/L (0.09 ~ 0.14);SDMA值分别为0.11(0.09 ~ 0.14)、0.12(0.09 ~ 0.15)、0.10µmol/L(0.09 ~ 0.16)。任何生物标志物在不同时间点之间均无统计学差异。这些发现表明,尽管哺乳期间存在生理氧化应激,但这些生物标志物在健康奶牛中保持稳定。因此,可能没有必要根据哺乳期建立不同的参考范围。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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