Molecular Detection of Enterocytozoon bieneusi in Free-Range Sheep and Domestic Dogs from the Greater Hinggan Mountains Area of China.

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Yanyan Jiang, Zhongying Yuan, Xu Wang, Hongling Zhang, Hao Zhou, Weiping Wu, Yujuan Shen, Jianping Cao
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Abstract

Enterocytozoon bieneusi (E. bieneusi) is a globally distributed microsporidian that infects both humans and animals. However, reports on its occurrence in free-ranging sheep and domestic dogs from rural areas are limited. The objective of this study was to investigate the prevalence and genotype distribution of E. bieneusi among freely grazing sheep and household dogs in the Hinggan Mountain region of China. A total of 235 fecal samples were collected, consisting of 95 from sheep and 140 from dogs, and screened for E. bieneusi by nested PCR targeting the internal transcribed spacer region (ITS) within the nuclear ribosomal DNA (rDNA) gene. The total infection rate of E. bieneusi amounted to 3.0% (7/235), with sheep exhibiting a rate of 5.3% (5/95) and dogs demonstrating a rate of 1.4% (2/140). Sequence analysis identified two known genotypes in sheep (BEB6 and NESH4, both within phylogenetic group 2) and one known genotype in dogs (CHN-F1, phylogenetic group 1), with CHN-F1 being reported in dogs for the first time. Importantly, the detection of BEB6, a genotype previously associated with human infection, suggests that sheep could act as a potential reservoir contributing to zoonotic transmission and environmental contamination. These findings expand knowledge of the genotype spectrum of E. bieneusi in rural animal populations and provide evidence of possible cross-species transmission between humans and the surveyed animals in the region.

大兴安岭地区散养羊和家犬中双胞虫的分子检测。
勿氏肠胞虫(勿氏肠胞虫)是一种全球分布的微孢子虫,可感染人和动物。然而,在农村地区自由放养的羊和家犬中发生的报告有限。本研究旨在调查中国兴安山区自由放牧羊和家养狗的比氏鄂鄂杆菌的流行情况和基因型分布。收集绵羊粪便95份,犬粪便140份,共235份,采用巢式PCR方法对核糖体DNA (rDNA)基因内转录间隔区(ITS)进行筛选。总感染率为3.0%(7/235),其中绵羊感染率为5.3%(5/95),犬感染率为1.4%(2/140)。序列分析在绵羊中鉴定出2种已知基因型(BEB6和NESH4,均属于系统发生组2),在犬中鉴定出1种已知基因型(CHN-F1,系统发生组1),其中CHN-F1首次在犬中报道。重要的是,先前与人类感染相关的基因型BEB6的检测表明,绵羊可能作为潜在的宿主,有助于人畜共患病传播和环境污染。这些发现扩大了对农村动物种群中布氏伊蚊基因型谱的认识,并提供了该地区人类与被调查动物之间可能存在跨物种传播的证据。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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