Synergistic Enhancement of Humoral and Cellular Immunity in Attenuated Live Vaccines Using Chemical Nanoadjuvants: Evaluation of Sustained-Release Formulations.

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Jianli Shi, Chang Liu, Chen Li, Jun Li
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Abstract

Achieving a balance between enhancing both humoral and cellular immunity while ensuring sustained release for attenuated live vaccines has long been a challenge in the vaccine industry. However, the incorporation of chemical materials as nanoadjuvants has emerged as a promising strategy to address this challenge. In this study, a novel nano-adjuvant with sustained release properties was developed by combining polyethylene glycol (PEG) and other biochemical and molecular biology agents with CpG. The efficacy of this adjuvant was assessed using the widely applied CSF attenuated live vaccine. The body temperature and relative daily weight gain results demonstrated the safety of the formulations. Importantly, the combination of CpG immune enhancers and sustained-release agents led to a synergistic augmentation of both humoral and cellular immune responses. Specifically, the combination of PEG20000 and benzoic acid, along with Seppic white oil, significantly enhanced humoral immunity. In contrast, the combination of total white oil, PEG6000 and benzoic acid demonstrated superior efficacy in promoting cellular immunity. This study offers important insights into enhancing vaccine efficacy through novel adjuvant combinations, which can be applied to the development of more effective vaccines in the future.

化学纳米佐剂协同增强减毒活疫苗的体液和细胞免疫:缓释制剂的评价
在确保减毒活疫苗持续释放的同时,如何在增强体液免疫和细胞免疫之间取得平衡,长期以来一直是疫苗行业面临的挑战。然而,结合化学材料作为纳米佐剂已经成为解决这一挑战的一个有希望的策略。本研究将聚乙二醇(PEG)等生物化学和分子生物学试剂与聚乙二醇(CpG)结合,制备了一种具有缓释特性的纳米佐剂。使用广泛应用的脑脊液减毒活疫苗评估该佐剂的效力。体温和相对日增重结果证明了配方的安全性。重要的是,CpG免疫增强剂和缓释剂的组合导致体液和细胞免疫反应的协同增强。具体而言,PEG20000与苯甲酸结合,以及西洋参白油,显著增强体液免疫。相比之下,总白油、PEG6000和苯甲酸联合使用对细胞免疫的促进效果更佳。该研究为通过新型佐剂组合增强疫苗效力提供了重要见解,可用于未来开发更有效的疫苗。
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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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