{"title":"大兴安岭地区散养羊和家犬中双胞虫的分子检测。","authors":"Yanyan Jiang, Zhongying Yuan, Xu Wang, Hongling Zhang, Hao Zhou, Weiping Wu, Yujuan Shen, Jianping Cao","doi":"10.3390/vetsci12090897","DOIUrl":null,"url":null,"abstract":"<p><p><i>Enterocytozoon bieneusi</i> (<i>E. bieneusi</i>) is a globally distributed microsporidian that infects both humans and animals. However, reports on its occurrence in free-ranging sheep and domestic dogs from rural areas are limited. The objective of this study was to investigate the prevalence and genotype distribution of <i>E. bieneusi</i> among freely grazing sheep and household dogs in the Hinggan Mountain region of China. A total of 235 fecal samples were collected, consisting of 95 from sheep and 140 from dogs, and screened for <i>E. bieneusi</i> by nested PCR targeting the internal transcribed spacer region (ITS) within the nuclear ribosomal DNA (rDNA) gene. The total infection rate of <i>E. bieneusi</i> amounted to 3.0% (7/235), with sheep exhibiting a rate of 5.3% (5/95) and dogs demonstrating a rate of 1.4% (2/140). Sequence analysis identified two known genotypes in sheep (BEB6 and NESH4, both within phylogenetic group 2) and one known genotype in dogs (CHN-F1, phylogenetic group 1), with CHN-F1 being reported in dogs for the first time. Importantly, the detection of BEB6, a genotype previously associated with human infection, suggests that sheep could act as a potential reservoir contributing to zoonotic transmission and environmental contamination. These findings expand knowledge of the genotype spectrum of <i>E. bieneusi</i> in rural animal populations and provide evidence of possible cross-species transmission between humans and the surveyed animals in the region.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474382/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular Detection of <i>Enterocytozoon bieneusi</i> in Free-Range Sheep and Domestic Dogs from the Greater Hinggan Mountains Area of China.\",\"authors\":\"Yanyan Jiang, Zhongying Yuan, Xu Wang, Hongling Zhang, Hao Zhou, Weiping Wu, Yujuan Shen, Jianping Cao\",\"doi\":\"10.3390/vetsci12090897\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Enterocytozoon bieneusi</i> (<i>E. bieneusi</i>) is a globally distributed microsporidian that infects both humans and animals. However, reports on its occurrence in free-ranging sheep and domestic dogs from rural areas are limited. The objective of this study was to investigate the prevalence and genotype distribution of <i>E. bieneusi</i> among freely grazing sheep and household dogs in the Hinggan Mountain region of China. A total of 235 fecal samples were collected, consisting of 95 from sheep and 140 from dogs, and screened for <i>E. bieneusi</i> by nested PCR targeting the internal transcribed spacer region (ITS) within the nuclear ribosomal DNA (rDNA) gene. The total infection rate of <i>E. bieneusi</i> amounted to 3.0% (7/235), with sheep exhibiting a rate of 5.3% (5/95) and dogs demonstrating a rate of 1.4% (2/140). Sequence analysis identified two known genotypes in sheep (BEB6 and NESH4, both within phylogenetic group 2) and one known genotype in dogs (CHN-F1, phylogenetic group 1), with CHN-F1 being reported in dogs for the first time. Importantly, the detection of BEB6, a genotype previously associated with human infection, suggests that sheep could act as a potential reservoir contributing to zoonotic transmission and environmental contamination. These findings expand knowledge of the genotype spectrum of <i>E. bieneusi</i> in rural animal populations and provide evidence of possible cross-species transmission between humans and the surveyed animals in the region.</p>\",\"PeriodicalId\":23694,\"journal\":{\"name\":\"Veterinary Sciences\",\"volume\":\"12 9\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474382/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Sciences\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3390/vetsci12090897\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Sciences","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/vetsci12090897","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Molecular Detection of Enterocytozoon bieneusi in Free-Range Sheep and Domestic Dogs from the Greater Hinggan Mountains Area of China.
Enterocytozoon bieneusi (E. bieneusi) is a globally distributed microsporidian that infects both humans and animals. However, reports on its occurrence in free-ranging sheep and domestic dogs from rural areas are limited. The objective of this study was to investigate the prevalence and genotype distribution of E. bieneusi among freely grazing sheep and household dogs in the Hinggan Mountain region of China. A total of 235 fecal samples were collected, consisting of 95 from sheep and 140 from dogs, and screened for E. bieneusi by nested PCR targeting the internal transcribed spacer region (ITS) within the nuclear ribosomal DNA (rDNA) gene. The total infection rate of E. bieneusi amounted to 3.0% (7/235), with sheep exhibiting a rate of 5.3% (5/95) and dogs demonstrating a rate of 1.4% (2/140). Sequence analysis identified two known genotypes in sheep (BEB6 and NESH4, both within phylogenetic group 2) and one known genotype in dogs (CHN-F1, phylogenetic group 1), with CHN-F1 being reported in dogs for the first time. Importantly, the detection of BEB6, a genotype previously associated with human infection, suggests that sheep could act as a potential reservoir contributing to zoonotic transmission and environmental contamination. These findings expand knowledge of the genotype spectrum of E. bieneusi in rural animal populations and provide evidence of possible cross-species transmission between humans and the surveyed animals in the region.
期刊介绍:
Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.