南方土种黄牛FABP3和FABP4基因与乳成分和脂肪酸谱变化的关系

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Mervan Bayraktar, Serap Göncü, Atalay Ergül, Recep Karaman, Bahri Devrim Özcan, Şerife Ergül, Celile Aylin Oluk, Özgül Anitaş, Ahmet Bayram, Mohammed Baqur S Al-Shuhaib
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引用次数: 0

摘要

脂肪酸结合蛋白FABP3和FABP4作为细胞内脂质伴侣影响脂肪酸在乳腺组织中的转运和代谢,这些基因的遗传变异可能影响乳成分。我们研究了200头泌乳奶牛FABP3和FABP4多态性与乳成分和脂肪酸谱的关系。对每头奶牛的DNA进行pcr扩增,并对FABP3和FABP4进行sanger测序;利用线性模型对相关协变量进行校正,测试基因型与乳脂肪酸浓度和标准成分性状的相关性。我们在FABP3中检测到一个错义变体(C . 3656g > a; p.Val45Met),在FABP4中检测到一个内含子SNP (g.3509T > C)。FABP3 p.Val45Met AA基因型与较高浓度的丁酸、棕榈酸、油酸和α-亚麻酸相关。具有FABP4 TC基因型的奶牛肉芽素、γ-亚麻酸、共轭亚油酸和花生四酸水平升高,无脂干物质、蛋白质和乳糖含量增加。硅分析为p.Val45Met的结构效应提供了不同的证据,分子对接表明配体对几种脂肪酸的亲和力发生了改变,剪接位点预测表明g.c 3509t > C可能参与转录过程的改变。这些变异构成NSY牛乳脂肪酸组成的候选标记;建议在独立队列中进行重复和功能验证,以确认其对改善牛奶质量的效用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association Between FABP3 and FABP4 Genes with Changes in Milk Composition and Fatty Acid Profiles in the Native Southern Yellow Cattle Breed.

Fatty acid binding proteins FABP3 and FABP4 act as intracellular lipid chaperones that influence fatty acid transport and metabolism in mammary tissue, and genetic variation in these genes may affect milk composition. We examined the associations between FABP3 and FABP4 polymorphisms and milk composition and fatty acid profiles in 200 lactating Native Southern Yellow (NSY) cows. DNA from each cow was PCR-amplified and Sanger-sequenced for FABP3 and FABP4; genotypes were tested for their association with milk fatty acid concentrations and standard composition traits using linear models adjusted for relevant covariates. We detected a missense variant in FABP3 (c.3656G > A; p.Val45Met) and an intronic SNP in FABP4 (g.3509T > C). The FABP3 p.Val45Met AA genotype was associated with higher concentrations of butyric, palmitic, oleic, and α-linolenic acids. Cows with the FABP4 TC genotype exhibited elevated levels of myristoleic, γ-linolenic, conjugated linoleic, and arachidic acids, along with increased fat-free dry matter, protein, and lactose. In silico analyses provided mixed evidence for the structural effects of p.Val45Met, molecular docking suggested altered ligand affinity for several fatty acids, and splice site prediction implicated g.3509T > C in possible transcript processing changes. These variants constitute candidate markers for milk fatty acid composition in NSY cattle; replication in independent cohorts and functional validation are recommended to confirm their utility for milk quality improvement.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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