Elshymaa A Abdelnaby, Hossam R El-Sherbiny, Abdulrhman K Alhaider, Mohamed Marzok, Ibrahim A Emam
{"title":"免疫抑制素对骆驼睾丸形态、回声分析、精液质量和与激素有关的血管化的影响。","authors":"Elshymaa A Abdelnaby, Hossam R El-Sherbiny, Abdulrhman K Alhaider, Mohamed Marzok, Ibrahim A Emam","doi":"10.3390/vetsci12090896","DOIUrl":null,"url":null,"abstract":"<p><p>This study aims to demonstrate whether immunization against inhibin could alters testicular echotexture (TE), hemodynamics (resistance and pulstalilty index [RI and PI], testicular blood flow volume [TBFV]), and semen picture. Senile male camels were immunized actively against inhibin alpha subunit (immunized males; n = 5; subcutaneous route; 1 mL) or non immunized (control males; n = 5). The injection was four times with four weeks intervals. Semen was collected by electroejaculator once per week. Blood sampling, ultrasonography, and hormonal assaying was performed once per week. The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone (T), and nitric oxide (NO) were calculated. Regarding control camels, the FSH levels were elevated (<i>p</i> < 0.01) in immunized males weeks 6-9 in August. Both E2 and NO were increased in the same group at weeks 7-11 with the highest level in week 10 for E2 (17.01 ± 0.11 pg/mL) in September and in week 9 for NO (44.66 ± 0.15 µmol/L). The Doppler indices RI and PI were declined (<i>p</i> < 0.01) in immunized camels in weeks 7-11, while the TBFV was elevated (<i>p</i> < 0.01). A marked elevation (<i>p</i> < 0.05) in testicular volume was noticed in the immunized group with a decline in the TE in week 9. The sperm cell concentration and viability were elevated (<i>p</i> < 0.01) in the immunized group. In conclusion, the active immunization against inhibin in senile camels shows a positive effect via improvement of testicular hemodynamics, flow volume, testicular volume, FSH, E2, NO, and sperm cell concentration with viability %, and alterations in both Doppler indices with the TE.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 9","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474288/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of Immunization Against Inhibin on Camel Testicular Morphometry, Echotexture Analysis, Semen Quality, and Vascularization in Relation to Hormonal Aspect.\",\"authors\":\"Elshymaa A Abdelnaby, Hossam R El-Sherbiny, Abdulrhman K Alhaider, Mohamed Marzok, Ibrahim A Emam\",\"doi\":\"10.3390/vetsci12090896\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aims to demonstrate whether immunization against inhibin could alters testicular echotexture (TE), hemodynamics (resistance and pulstalilty index [RI and PI], testicular blood flow volume [TBFV]), and semen picture. Senile male camels were immunized actively against inhibin alpha subunit (immunized males; n = 5; subcutaneous route; 1 mL) or non immunized (control males; n = 5). The injection was four times with four weeks intervals. Semen was collected by electroejaculator once per week. Blood sampling, ultrasonography, and hormonal assaying was performed once per week. The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone (T), and nitric oxide (NO) were calculated. Regarding control camels, the FSH levels were elevated (<i>p</i> < 0.01) in immunized males weeks 6-9 in August. Both E2 and NO were increased in the same group at weeks 7-11 with the highest level in week 10 for E2 (17.01 ± 0.11 pg/mL) in September and in week 9 for NO (44.66 ± 0.15 µmol/L). The Doppler indices RI and PI were declined (<i>p</i> < 0.01) in immunized camels in weeks 7-11, while the TBFV was elevated (<i>p</i> < 0.01). A marked elevation (<i>p</i> < 0.05) in testicular volume was noticed in the immunized group with a decline in the TE in week 9. The sperm cell concentration and viability were elevated (<i>p</i> < 0.01) in the immunized group. In conclusion, the active immunization against inhibin in senile camels shows a positive effect via improvement of testicular hemodynamics, flow volume, testicular volume, FSH, E2, NO, and sperm cell concentration with viability %, and alterations in both Doppler indices with the TE.</p>\",\"PeriodicalId\":23694,\"journal\":{\"name\":\"Veterinary Sciences\",\"volume\":\"12 9\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474288/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Sciences\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3390/vetsci12090896\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Sciences","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/vetsci12090896","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Effect of Immunization Against Inhibin on Camel Testicular Morphometry, Echotexture Analysis, Semen Quality, and Vascularization in Relation to Hormonal Aspect.
This study aims to demonstrate whether immunization against inhibin could alters testicular echotexture (TE), hemodynamics (resistance and pulstalilty index [RI and PI], testicular blood flow volume [TBFV]), and semen picture. Senile male camels were immunized actively against inhibin alpha subunit (immunized males; n = 5; subcutaneous route; 1 mL) or non immunized (control males; n = 5). The injection was four times with four weeks intervals. Semen was collected by electroejaculator once per week. Blood sampling, ultrasonography, and hormonal assaying was performed once per week. The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), testosterone (T), and nitric oxide (NO) were calculated. Regarding control camels, the FSH levels were elevated (p < 0.01) in immunized males weeks 6-9 in August. Both E2 and NO were increased in the same group at weeks 7-11 with the highest level in week 10 for E2 (17.01 ± 0.11 pg/mL) in September and in week 9 for NO (44.66 ± 0.15 µmol/L). The Doppler indices RI and PI were declined (p < 0.01) in immunized camels in weeks 7-11, while the TBFV was elevated (p < 0.01). A marked elevation (p < 0.05) in testicular volume was noticed in the immunized group with a decline in the TE in week 9. The sperm cell concentration and viability were elevated (p < 0.01) in the immunized group. In conclusion, the active immunization against inhibin in senile camels shows a positive effect via improvement of testicular hemodynamics, flow volume, testicular volume, FSH, E2, NO, and sperm cell concentration with viability %, and alterations in both Doppler indices with the TE.
期刊介绍:
Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.