蛋蛋混合益生菌促进鸡胚卵黄囊巨噬细胞和粒细胞募集,增加新生血管生成。

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Lucia Biagini, Stefano Pesaro, Livio Galosi, Donatella Volpatti, Danilo De Bellis, Alessandra Roncarati, Alessandra Gavazza, Giacomo Rossi
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引用次数: 0

摘要

卵黄囊(YS)在禽类胚胎发育中起着关键作用,对造血和免疫成熟都有贡献。本研究旨在评估鸡蛋中添加一种商业益生菌混合物(Slab51®)对鸡胚胎YS细胞动力学的影响。在胚胎日(ED) 18时,分别给Ross308肉鸡蛋注射益生菌悬液(P)和无菌生理盐水(C)。分别于接种后8、12、24和36 h采集YS组织进行组织学和免疫组织化学分析。益生菌处理过的胚胎在YS内的粒细胞灶明显减少,可能反映了成熟粒细胞外周迁移的增强。同时,在益生菌组中观察到Iba-1+巨噬细胞的进行性增加,提示巨噬细胞分化加速。免疫表型分析显示M1-iNOS+巨噬细胞在所有时间点上都占主导地位,尽管在益生菌处理的胚胎中,36 h检测到M2-CD204+巨噬细胞显著增加,这表明可能存在抗炎转变。此外,益生菌组中CD31+内皮细胞的显著增加支持新血管生成的相关增加。这些研究结果表明,在蛋内给予益生菌可以通过促进早期巨噬细胞募集、巨噬细胞极化和血管重塑来调节YS微环境。据我们所知,这是第一个证明益生菌诱导鸡胚YS结构和免疫学改变的报告。这些结果为益生菌的早期免疫调节作用提供了新的见解,并突出了YS作为胚胎发生过程中宿主-益生菌相互作用的关键介质的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhanced Macrophage and Granulocytic Recruitment with Increased Neo-Angiogenesis in Chicken Embryo Yolk Sac Following In Ovo Probiotic Blend Administration.

The yolk sac (YS) plays a pivotal role in avian embryonic development, contributing to both haematopoiesis and immune maturation. This study aimed to evaluate the effects of in ovo administration of a commercial probiotic blend (Slab51®) on YS cellular dynamics in chicken embryos. At embryonic day (ED) 18, Ross308 broiler eggs were injected with either the probiotic suspension (P) or sterile saline solution (C). YS tissues were sampled at 8, 12, 24 and 36 h post-inoculation for histological and immunohistochemical analysis. Probiotic-treated embryos exhibited a significant reduction in granulocytic foci within the YS, potentially reflecting enhanced peripheral migration of mature granulocytes. Concurrently, a progressive increase in Iba-1+ macrophages was observed in the probiotic group, suggesting accelerated macrophage differentiation. Immunophenotyping revealed a predominance of M1-iNOS+ macrophages across all timepoints, although a significant increase in M2-CD204+ macrophages was detected at 36 h in probiotic-treated embryos, indicating a possible anti-inflammatory shift. Furthermore, a marked increase in CD31+ endothelial cells in the probiotic group supports an associated rise in neo-angiogenesis. These findings suggest that in ovo probiotic administration modulates the YS microenvironment by promoting early macrophage recruitment, macrophage polarization and vascular remodeling. To our knowledge, this is the first report to demonstrate probiotic-induced structural and immunological alterations in the chicken embryo YS. These results provide novel insights into the early immunomodulatory effects of probiotics and highlight the potential of the YS as a key mediator of host-probiotic interaction during embryogenesis.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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