Biophysical reviews最新文献_第5页

The flexible chain: regulation of structure and activity of ETC complexes defines rate of ATP synthesis and sites of superoxide generation. 柔性链：ETC配合物的结构和活性调控决定了ATP合成速率和超氧化物生成位点。

IF 4.9

Biophysical reviews Pub Date : 2025-01-25 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-025-01270-5

Zhanna V Bochkova, Adil A Baizhumanov, Alexander I Yusipovich, Kseniia I Morozova, Evelina I Nikelshparg, Anna A Fedotova, Alisa B Tiaglik, Yu Xu, Alexey R Brazhe, Georgy V Maksimov, Dmitry S Bilan, Yuliya V Khramova, Evgeniya Yu Parshina, Nadezda A Brazhe

{"title":"The flexible chain: regulation of structure and activity of ETC complexes defines rate of ATP synthesis and sites of superoxide generation.","authors":"Zhanna V Bochkova, Adil A Baizhumanov, Alexander I Yusipovich, Kseniia I Morozova, Evelina I Nikelshparg, Anna A Fedotova, Alisa B Tiaglik, Yu Xu, Alexey R Brazhe, Georgy V Maksimov, Dmitry S Bilan, Yuliya V Khramova, Evgeniya Yu Parshina, Nadezda A Brazhe","doi":"10.1007/s12551-025-01270-5","DOIUrl":"10.1007/s12551-025-01270-5","url":null,"abstract":"This review highlights current insights into the regulation of the mitochondrial respiratory chain (electron transport chain, ETC) activity. The regulation of ETC properties optimizes ATP synthesis and controls the generation of the superoxide anion radical (O2 •-) which can be converted into other reactive oxygen species (ROS) playing a dual role by initiating signaling cascades or contributing to oxidative stress. We examine how ETC activity is influenced by the structure and conformation of its complexes, their allosteric or post-translational modifications, and their interactions with membrane lipids. The formation and function of supercomplexes, as well as their cell-type-specific characteristics, are also discussed, alongside with the role of intracellular Ca2+ concentration in the modulation of ETC activity. Furthermore, we discuss mechanisms and sites of O2 •- generation within ETC complexes, O2 •- fate in the mitochondrial matrix, and the impact of cytochrome c (Cyt c) conformation and allosteric modifications on ETC function. Finally, we discuss various abnormalities in ETC complexes, emphasizing their relevance to mitochondrial dysfunction and disease.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"55-88"},"PeriodicalIF":4.9,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Half a century of biophysics: a comparison of participant statistics from the 6th and 21st IUPAB congresses. 半个世纪的生物物理学：第六届和第21届IUPAB大会的参与者统计比较。

IF 4.9

Biophysical reviews Pub Date : 2025-01-24 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-025-01268-z

Kuniaki Nagayama, Mikio Kataoka

引用次数: 0

On the distance to the transition state of protein folding in optical tweezers experiments. 光镊实验中蛋白质折叠过渡态距离的研究。

IF 4.9

Biophysical reviews Pub Date : 2025-01-09 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01264-9

Camila G Corrêa, Christian A M Wilson

{"title":"On the distance to the transition state of protein folding in optical tweezers experiments.","authors":"Camila G Corrêa, Christian A M Wilson","doi":"10.1007/s12551-024-01264-9","DOIUrl":"10.1007/s12551-024-01264-9","url":null,"abstract":"The distance to the transition state ( <math> <msup><mrow><mi>x</mi></mrow> <mo>‡</mo></msup> </math> ) is an important parameter for understanding the energy landscape of chemical reactions. In protein folding, <math> <msup><mrow><mi>x</mi></mrow> <mo>‡</mo></msup> </math> represents the distance to the high energy structure between folded and unfolded states. This correlates with the deformation of the protein as it crosses the energy barrier defining its rigidity. This parameter can be determined by unfolding the protein, analyzing the kinetics of unfolding and refolding, and fitting the data to various models. An approach to determine the <math> <msup><mrow><mi>x</mi></mrow> <mo>‡</mo></msup> </math> is using force as a way to tilt the energy landscape. Force spectroscopy studies, particularly at the single-molecule level, offer a powerful approach for this purpose. One of these techniques is optical tweezers, which allow the application of force by pulling on a bead attached to the protein via spacers, thereby unfolding it. This method provides measurements of force and distance between the folded and unfolded states of the protein. By analyzing force histograms, we can apply different models as the phenomenological Bell-Evans or Kramers theory-based models. Additionally, an alternative direct approach involves summing the distances to the transition state to fit the data of the distance of total protein unfolding. Using this approach, we can plot force versus distance and obtain the <math> <msup><mrow><mi>x</mi></mrow> <mo>‡</mo></msup> </math> and the energy to the transition state from folded to unfolding and vice versa. Furthermore, these results can be correlated with elastic models, such as the worm-like chain model. By integrating these approaches, we can gain deeper insights into protein folding mechanisms.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"45-54"},"PeriodicalIF":4.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical Reviews: the IUPAB journal promoting biophysics on an international stage. 《生物物理评论》：IUPAB在国际舞台上推广生物物理学的期刊。

IF 4.9

Biophysical reviews Pub Date : 2025-01-03 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01266-7

Wilma K Olson

引用次数: 0

Advancements in techniques for human iPSC-derived cardiomyocytes maturation: mechanical and electrical stimulation approaches. 人类ipsc衍生心肌细胞成熟技术的进展：机械和电刺激方法。

IF 4.9

Biophysical reviews Pub Date : 2025-01-03 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01267-6

Yinsheng Lu, Yufeng Liu, Yumeng Yan, Saba Fooladi, Yibing Qyang

{"title":"Advancements in techniques for human iPSC-derived cardiomyocytes maturation: mechanical and electrical stimulation approaches.","authors":"Yinsheng Lu, Yufeng Liu, Yumeng Yan, Saba Fooladi, Yibing Qyang","doi":"10.1007/s12551-024-01267-6","DOIUrl":"10.1007/s12551-024-01267-6","url":null,"abstract":"The maturation of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is pivotal for their potent application in regenerative medicine, drug screening, and disease modeling. While the emergence of hiPSC-CMs solved the inadequacy of cardiomyocytes in cardiovascular research, they frequently remain immature: more closely resembling fetal rather than adult cardiomyocytes. This immaturity limits their functional utility in both laboratorial and clinical practices. Early methods focused on optimizing culture media with hormones and growth factors to regulate gene transcription related to structural proteins and metabolic enzymes. Subsequently, mechanical training platforms such as static and cyclic stretching were developed to enhance sarcomere alignment and protein expression. Electrical pacing has also been implemented as a crucial method to improve electrophysiological properties by synchronizing contractions and enhancing ion channel expression. The integration of these techniques, along with gene editing and co-culture systems, has significantly advanced the maturation process of hiPSC-CMs. Our review comprehensively explores the advancements in mechanical and electrical stimulation techniques for promoting the maturation of hiPSC-CMs and provides valuable insights for developing effective maturation protocols.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"169-183"},"PeriodicalIF":4.9,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing living cell dynamics and molecular interactions using atomic force microscopy. 利用原子力显微镜探测活细胞动力学和分子相互作用。

IF 4.9

Biophysical reviews Pub Date : 2024-12-28 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01258-7

David Alsteens

引用次数: 0

Ionic liquids as stabilisers of therapeutic protein formulations: a review of insulin and monoclonal antibodies. 离子液体作为治疗性蛋白质制剂的稳定剂：胰岛素和单克隆抗体的综述。

IF 4.9

Biophysical reviews Pub Date : 2024-12-26 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01261-y

Samuel Tien, Veysel Kayser

{"title":"Ionic liquids as stabilisers of therapeutic protein formulations: a review of insulin and monoclonal antibodies.","authors":"Samuel Tien, Veysel Kayser","doi":"10.1007/s12551-024-01261-y","DOIUrl":"10.1007/s12551-024-01261-y","url":null,"abstract":"Therapeutic proteins such as insulin and monoclonal antibodies (mAbs) have become an essential part of the modern healthcare system and play a crucial role in the treatment of various diseases including cancer and autoimmune disorders. However, their long-term stability is a significant concern, affecting efficacy, shelf-life, and safety. Ionic liquids (ILs) have emerged as promising additives to enhance protein stability and address the aforementioned issues. Indeed, recent studies indicate that biocompatible ILs, particularly choline-based ILs, have significant potential to improve stability while preserving proteins' functionality. For instance, choline valinate has been shown to increase the melting temperature of insulin by almost 13 °C (Judy and Kishore Biochimie 207:20-32, 2023), while choline dihydrogen phosphate has increased the melting temperature of trastuzumab by over 21 °C (Reslan et al. Chem Commun 54:10622-10625, 2018). However, it is worth noting that the use of some ILs introduces a complex trade-off: while they can increase thermal stability, they may also promote protein unfolding, thereby reducing conformational stability. Moreover, selecting the most suitable IL and its optimal concentration is challenging, as different protein formulations may exhibit varying effects. This review provides a comprehensive overview of the existing literature on ILs as stabilisers for insulin and mAbs, documenting specific IL-protein combinations and conditions to identify potential future stabilising agents for biologics in general.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"89-101"},"PeriodicalIF":4.9,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical assays to test cellular mechanosensing: moving towards high throughput. 测试细胞机械传感的生物物理分析：迈向高通量。

IF 4.9

Biophysical reviews Pub Date : 2024-12-20 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01263-w

Marta Cubero-Sarabia, Anna Maria Kapetanaki, Massimo Vassalli

引用次数: 0

Hydrogel models of pancreatic adenocarcinoma to study cell mechanosensing. 胰腺癌水凝胶模型研究细胞力学传感。

IF 4.9

Biophysical reviews Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01265-8

M Walker, J P Morton

{"title":"Hydrogel models of pancreatic adenocarcinoma to study cell mechanosensing.","authors":"M Walker, J P Morton","doi":"10.1007/s12551-024-01265-8","DOIUrl":"10.1007/s12551-024-01265-8","url":null,"abstract":"Pancreatic adenocarcinoma (PDAC) is the predominant form of pancreatic cancer and one of the leading causes of cancer-related death worldwide, with an extremely poor prognosis after diagnosis. High mortality from PDAC arises partly due to late diagnosis resulting from a lack of early-stage biomarkers and due to chemotherapeutic drug resistance, which arises from a highly fibrotic stromal response known as desmoplasia. Desmoplasia alters tissue mechanics, which triggers changes in cell mechanosensing and leads to dysregulated transcriptional activity and disease phenotypes. Hydrogels are effective in vitro models to mimic mechanical changes in tissue mechanics during PDAC progression and to study the influence of these changes on mechanosensitive cell responses. Despite the complex biophysical changes that occur within the PDAC microenvironment, carefully designed hydrogels can very closely recapitulate these properties during PDAC progression. Hydrogels are relatively inexpensive, highly reproducible and can be designed in a humanised manner to increase their relevance for human PDAC studies. In vivo models have some limitations, including species-species differences, high variability, expense and legal/ethical considerations, which make hydrogel models a promising alternative. Here, we comprehensively review recent advancements in hydrogel bioengineering for developing our fundamental understanding of mechanobiology in PDAC, which is critical for informing advanced therapeutics.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 6","pages":"851-870"},"PeriodicalIF":4.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational and experimental advances in liver-on-a-chip technology for cancer research: a systematic review. 用于癌症研究的肝脏芯片技术的计算和实验进展：系统综述。

IF 4.9

Biophysical reviews Pub Date : 2024-12-14 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01260-z

Violeta Carvalho, Mariana Ferreira, Raquel O Rodrigues, Senhorinha F C F Teixeira, Rui A Lima

{"title":"Computational and experimental advances in liver-on-a-chip technology for cancer research: a systematic review.","authors":"Violeta Carvalho, Mariana Ferreira, Raquel O Rodrigues, Senhorinha F C F Teixeira, Rui A Lima","doi":"10.1007/s12551-024-01260-z","DOIUrl":"10.1007/s12551-024-01260-z","url":null,"abstract":"The integration of numerical simulations with Liver-on-a-Chip (LoC) technology offers an innovative approach for studying liver physiology and pathology, especially in the context of liver cancer. Numerical simulations facilitate the optimization of microfluidic devices' design and deepen the understanding of fluid flow and mass transfer. However, despite significant advancements, challenges such as replicating the full complexity of the liver microenvironment and scaling up for high-throughput screening persist. This systematic review explores the current advancements in LoC devices, with a particular emphasis on their combined use of numerical simulations and experimental studies in liver cancer research. A comprehensive search across multiple databases, including ScienceDirect, Wiley Online Library, Scopus, Springer Link, Web of Science, and PubMed, was conducted to gather relevant literature. Our findings indicate that the combination of both techniques in this field is still rare, resulting in a final selection of 13 original research papers. This review underscores the importance of continued interdisciplinary research to refine these technologies and enhance their application in personalized medicine and cancer therapy. By consolidating existing studies, this review aims to highlight key advancements, identify current challenges, and propose future directions for this rapidly evolving field.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"151-167"},"PeriodicalIF":4.9,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0