Biophysical reviews最新文献

Biophysical Reviews: a call for nominations to the 2026 Michèle Auger Award. 生物物理评论：2026年米歇尔·奥格奖提名征集。

IF 3.7

Biophysical reviews Pub Date : 2025-07-01 eCollection Date: 2025-06-01 DOI: 10.1007/s12551-025-01327-5

Wilma K Olson

引用次数: 0

The rheology and interfacial properties of biomolecular condensates. 生物分子凝聚物的流变学和界面特性。

IF 3.7

Biophysical reviews Pub Date : 2025-06-30 eCollection Date: 2025-06-01 DOI: 10.1007/s12551-025-01326-6

Huan Wang, Zheng Shi

引用次数: 0

Biophysical Reviews: worldwide advances in biophysics. 生物物理评论：世界范围内生物物理学的进展。

IF 3.7

Biophysical reviews Pub Date : 2025-06-25 eCollection Date: 2025-06-01 DOI: 10.1007/s12551-025-01325-7

Wilma K Olson

引用次数: 0

The SURFE²R (surface electrogenic event reader): from fundamental research to commercial product. SURFE2R（表面生电事件阅读器）：从基础研究到商业产品。

IF 3.7

Biophysical reviews Pub Date : 2025-06-16 eCollection Date: 2025-06-01 DOI: 10.1007/s12551-025-01322-w

Ronald J Clarke

{"title":"The SURFE2R (surface electrogenic event reader): from fundamental research to commercial product.","authors":"Ronald J Clarke","doi":"10.1007/s12551-025-01322-w","DOIUrl":"10.1007/s12551-025-01322-w","url":null,"abstract":"The purpose of this review is to describe the development of the Surface Electrogenic Event Reader (SURFE2R) instrument, from the discovery of its fundamental underlying principle of capacitive coupling of biological membranes in the late 1970s to the present-day commercial instrument, which since 2012 has been marketed by the company Nanion Technologies. The story of the SURFE2R's development is a prime example of the transfer of a concept from fundamental research into a commercial product for the benefit of society. The capacitive coupling detection method was first recognized and used in research into the reaction mechanism of the proton pump bacteriorhodopsin from purple membrane fragments of a Halobacterium. The modern instrument now has a much wider application to research on the mechanisms of pumps and transporters in general, in the screening of drugs targeting pumps and transporters, and in quantifying drug affinity to biological membranes. The instrument is, therefore, of potential interest to researchers in both academia and the pharmaceutical industry. Because the author has worked and interacted with most, if not all, of the scientists involved in the evolution of the SURFE2R, the article also provides personal insights into the lives and careers of the leading scientists involved: Peter Läuger, Ernst Bamberg, Klaus Fendler, Thiemo Gropp and Niels Fertig.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 3","pages":"809-827"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Practical measures for achieving greater alignment between scientific practice and meaningful research output. 在科学实践和有意义的研究成果之间实现更大一致性的实际措施。

IF 3.7

Biophysical reviews Pub Date : 2025-06-10 eCollection Date: 2025-06-01 DOI: 10.1007/s12551-025-01324-8

Damien Hall

引用次数: 0

Drug resistance and tumor heterogeneity: cells and ensembles. 耐药和肿瘤异质性：细胞和集合。

IF 3.7

Biophysical reviews Pub Date : 2025-05-31 eCollection Date: 2025-06-01 DOI: 10.1007/s12551-025-01320-y

Ruth Nussinov, Bengi Ruken Yavuz, Hyunbum Jang

{"title":"Drug resistance and tumor heterogeneity: cells and ensembles.","authors":"Ruth Nussinov, Bengi Ruken Yavuz, Hyunbum Jang","doi":"10.1007/s12551-025-01320-y","DOIUrl":"10.1007/s12551-025-01320-y","url":null,"abstract":"The population of cells that make up a tumor, and of their biomolecular conformational ensembles, are heterogeneous at all levels, genetic, epigenetic, and phenotypic. At the cellular level, tumor heterogeneity was described as the \"Rosetta Stone of therapy resistance.\" At the genetic level, tumors consist of divergent tumor (sub)clones. At the phenotypic level, their observed function, clinical attributes, and response to drugs vary. We suggest that the behavior and properties of populations of cells-and of populations of conformational states-are intrinsically connected. This is important. Considering the tumor's disruption of normal cellular processes clarifies why it is crucial to understand the ins and outs of its mechanistic molecular foundation. In reality, the propensities of the tumor's conformational states underly the proliferative potential of its cell populations. These propensities are determined by expression levels, driver mutations, and the tumor cells environment, collectively transforming tumor cells behavior and crucially, drug resistance. We suggest that propensities of the conformations, across the tumor space and over time, shape tumor heterogeneity, and cell plasticity. The conformational states that are preferentially visited can be viewed as phenotypic determinants, and their mutations and altered expression work by allosterically shifting the relative propensities, thus the cell phenotype. Physics (and chemistry) inspire the notion that living things must conform to fundamental laws of science, like dynamic landscapes. Dynamic conformational propensities are at the core of cell life, including tumor cells; their heterogeneity is the formidable, unmet drug resistance challenge.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 3","pages":"759-779"},"PeriodicalIF":3.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in microfluidic mixers for time-resolved structural biology with X-rays. 用于x射线时间分辨结构生物学的微流控混合器研究进展。

IF 3.7

Biophysical reviews Pub Date : 2025-05-23 eCollection Date: 2025-06-01 DOI: 10.1007/s12551-025-01321-x

Kara A Zielinski, Lois Pollack

{"title":"Advances in microfluidic mixers for time-resolved structural biology with X-rays.","authors":"Kara A Zielinski, Lois Pollack","doi":"10.1007/s12551-025-01321-x","DOIUrl":"10.1007/s12551-025-01321-x","url":null,"abstract":"Structural biology techniques that utilize X-rays have contributed in fundamental ways to our understanding of biological macromolecules, such as proteins and nucleic acids. In addition to static structures, recent advances now allow for the observation of molecular motions using X-rays, facilitated by the many technological developments in both sources and detectors. Leveraging these advances, new approaches have been demonstrated that capture structural dynamics, sometimes with very high spatial resolution. Among the most promising are time-resolved studies, which involve triggering a reaction and capturing snapshots of reaction progression at set time delays. This review focuses on one type of time-resolved experiments, mixing experiments, in which reactants are combined within microfluidic mixers to initiate a reaction. Microfluidic mixers are extremely versatile; different designs can be optimized for various reaction types and compatibility with different structural biology techniques. When compared to other time-resolved approaches, mixing experiments are suitable for the widest range of biological applications. This review provides an overview of the current state of the field, including a review of different mixer types, and offers practical considerations for designing and performing time-resolved mixing studies with various X-ray-based structural biology methods.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 3","pages":"781-807"},"PeriodicalIF":3.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive review of bacterial death mechanism on nanopillared nanostructured surfaces. 纳米柱状纳米结构表面细菌死亡机制综述。

IF 3.7

Biophysical reviews Pub Date : 2025-05-20 eCollection Date: 2025-06-01 DOI: 10.1007/s12551-025-01319-5

Dimuthu Wijethunge, Asha Mathew, Prasad K D V Yarlagadda

{"title":"Comprehensive review of bacterial death mechanism on nanopillared nanostructured surfaces.","authors":"Dimuthu Wijethunge, Asha Mathew, Prasad K D V Yarlagadda","doi":"10.1007/s12551-025-01319-5","DOIUrl":"10.1007/s12551-025-01319-5","url":null,"abstract":"Antibiotic resistance presents a significant global concern, worsened by overuse and limited development of new antibiotics. Medical implants, in particular, are increasingly susceptible to bacterial infections. To prevent biofilm formation on implants, it is essential to design specialized surface characteristics that either kill bacteria or inhibit their growth. Nanostructures resembling those found in nature, such as cicada wings, exhibit pronounced antibacterial efficacy. Drawing inspiration from these natural surfaces, artificial nanostructures made with similar features have demonstrated bactericidal effect. The bactericidal mechanism in nanostructures may seem simple, as the nanofeatures pierce through bacterial cells, leading to their death. However, research has shown that it is more complex and requires thorough investigation. Several studies indicate that while the bactericidal mechanism is initiated by mechanical contact, the precise killing process remains uncertain. Numerous experimental and theoretical investigations have aimed to elucidate the exact killing mechanism, yielding diverse conclusions and hypotheses, including cell death attributed to creep failure, motion-induced shear failure, apoptosis-induced programmed cell death and autolytic cell death, among others. This study undertakes a comprehensive review of all proposed death mechanisms. Moreover, it draws conclusions on the killing mechanism by meticulously analyzing the properties of bacterial membranes, their mechanosensing and adhesion mechanisms, energy-based models for bacterial adhesion, and experimental outcomes regarding the bactericidal efficacy of surfaces exhibiting diverse geometries.Graphical abstract: ","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 3","pages":"893-908"},"PeriodicalIF":3.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances and perspectives of MicroNeedles for biomedical applications. 微针在生物医学应用中的最新进展与展望。

IF 3.7

Biophysical reviews Pub Date : 2025-04-24 eCollection Date: 2025-06-01 DOI: 10.1007/s12551-025-01317-7

Renata Faria Maia, Pedro Machado, Raquel O Rodrigues, Vera Faustino, Helmut Schütte, Stefan Gassmann, Rui A Lima, Graça Minas

{"title":"Recent advances and perspectives of MicroNeedles for biomedical applications.","authors":"Renata Faria Maia, Pedro Machado, Raquel O Rodrigues, Vera Faustino, Helmut Schütte, Stefan Gassmann, Rui A Lima, Graça Minas","doi":"10.1007/s12551-025-01317-7","DOIUrl":"10.1007/s12551-025-01317-7","url":null,"abstract":"Microneedles (MN) technology has emerged as a transformative tool within the biomedical field, offering innovative solutions to challenges in drug delivery, diagnostics, and therapeutic applications. This review article provides an in-depth exploration of the diverse perspectives and applications of MNs, shedding light on their pivotal role in shaping the future of biomedical research and clinical practice. It begins by elucidating the fundamental principles of MNs: design, fabrication techniques, and materials, highlighting their capacity for minimally invasive access to the skin and underlying tissues. These attributes have driven advancements in transdermal drug delivery, facilitating precise and controlled administration of therapeutics, vaccines, and biologics, thus improving patient compliance and treatment outcomes. Furthermore, this review investigates the growing range of applications for MNs, including biomarker extraction, interstitial fluid (ISF) analysis, and continuous glucose monitoring. MNs enable real-time and minimally invasive monitoring of biochemical markers and have the potential to revolutionize disease diagnostics, personalized medicine, and wellness monitoring. Their compatibility with microfluidic systems further enhances their potential for point-of-care testing. This review serves as a comprehensive guide, highlighting the breadth of opportunities and challenges in leveraging MNs to improve healthcare outcomes and emphasizing the need for continued research and development in this dynamic field.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 3","pages":"909-928"},"PeriodicalIF":3.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144727848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical reviews (ISSUE 2 2025): LAFeBS, alive, kicking, and growing: the story continues... 生物物理评论（2025年第2期）：LAFeBS，活着，踢，成长：故事还在继续……

IF 4.9

Biophysical reviews Pub Date : 2025-04-23 eCollection Date: 2025-04-01 DOI: 10.1007/s12551-025-01315-9

R Daniel Peluffo, Rosangela Itri, Leandro Rs Barbosa, Silvia Del Valle Alonso, Francisco L González Flecha

引用次数: 0