Biophysical reviews最新文献_第2页

Advancements in techniques for human iPSC-derived cardiomyocytes maturation: mechanical and electrical stimulation approaches.

IF 4.9

Biophysical reviews Pub Date : 2025-01-03 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01267-6

Yinsheng Lu, Yufeng Liu, Yumeng Yan, Saba Fooladi, Yibing Qyang

{"title":"Advancements in techniques for human iPSC-derived cardiomyocytes maturation: mechanical and electrical stimulation approaches.","authors":"Yinsheng Lu, Yufeng Liu, Yumeng Yan, Saba Fooladi, Yibing Qyang","doi":"10.1007/s12551-024-01267-6","DOIUrl":"10.1007/s12551-024-01267-6","url":null,"abstract":"The maturation of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is pivotal for their potent application in regenerative medicine, drug screening, and disease modeling. While the emergence of hiPSC-CMs solved the inadequacy of cardiomyocytes in cardiovascular research, they frequently remain immature: more closely resembling fetal rather than adult cardiomyocytes. This immaturity limits their functional utility in both laboratorial and clinical practices. Early methods focused on optimizing culture media with hormones and growth factors to regulate gene transcription related to structural proteins and metabolic enzymes. Subsequently, mechanical training platforms such as static and cyclic stretching were developed to enhance sarcomere alignment and protein expression. Electrical pacing has also been implemented as a crucial method to improve electrophysiological properties by synchronizing contractions and enhancing ion channel expression. The integration of these techniques, along with gene editing and co-culture systems, has significantly advanced the maturation process of hiPSC-CMs. Our review comprehensively explores the advancements in mechanical and electrical stimulation techniques for promoting the maturation of hiPSC-CMs and provides valuable insights for developing effective maturation protocols.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"169-183"},"PeriodicalIF":4.9,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing living cell dynamics and molecular interactions using atomic force microscopy. 利用原子力显微镜探测活细胞动力学和分子相互作用。

IF 4.9

Biophysical reviews Pub Date : 2024-12-28 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01258-7

David Alsteens

引用次数: 0

Ionic liquids as stabilisers of therapeutic protein formulations: a review of insulin and monoclonal antibodies.

IF 4.9

Biophysical reviews Pub Date : 2024-12-26 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01261-y

Samuel Tien, Veysel Kayser

{"title":"Ionic liquids as stabilisers of therapeutic protein formulations: a review of insulin and monoclonal antibodies.","authors":"Samuel Tien, Veysel Kayser","doi":"10.1007/s12551-024-01261-y","DOIUrl":"10.1007/s12551-024-01261-y","url":null,"abstract":"Therapeutic proteins such as insulin and monoclonal antibodies (mAbs) have become an essential part of the modern healthcare system and play a crucial role in the treatment of various diseases including cancer and autoimmune disorders. However, their long-term stability is a significant concern, affecting efficacy, shelf-life, and safety. Ionic liquids (ILs) have emerged as promising additives to enhance protein stability and address the aforementioned issues. Indeed, recent studies indicate that biocompatible ILs, particularly choline-based ILs, have significant potential to improve stability while preserving proteins' functionality. For instance, choline valinate has been shown to increase the melting temperature of insulin by almost 13 °C (Judy and Kishore Biochimie 207:20-32, 2023), while choline dihydrogen phosphate has increased the melting temperature of trastuzumab by over 21 °C (Reslan et al. Chem Commun 54:10622-10625, 2018). However, it is worth noting that the use of some ILs introduces a complex trade-off: while they can increase thermal stability, they may also promote protein unfolding, thereby reducing conformational stability. Moreover, selecting the most suitable IL and its optimal concentration is challenging, as different protein formulations may exhibit varying effects. This review provides a comprehensive overview of the existing literature on ILs as stabilisers for insulin and mAbs, documenting specific IL-protein combinations and conditions to identify potential future stabilising agents for biologics in general.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"89-101"},"PeriodicalIF":4.9,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical assays to test cellular mechanosensing: moving towards high throughput. 测试细胞机械传感的生物物理分析：迈向高通量。

IF 4.9

Biophysical reviews Pub Date : 2024-12-20 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01263-w

Marta Cubero-Sarabia, Anna Maria Kapetanaki, Massimo Vassalli

引用次数: 0

Hydrogel models of pancreatic adenocarcinoma to study cell mechanosensing. 胰腺癌水凝胶模型研究细胞力学传感。

IF 4.9

Biophysical reviews Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01265-8

M Walker, J P Morton

{"title":"Hydrogel models of pancreatic adenocarcinoma to study cell mechanosensing.","authors":"M Walker, J P Morton","doi":"10.1007/s12551-024-01265-8","DOIUrl":"10.1007/s12551-024-01265-8","url":null,"abstract":"Pancreatic adenocarcinoma (PDAC) is the predominant form of pancreatic cancer and one of the leading causes of cancer-related death worldwide, with an extremely poor prognosis after diagnosis. High mortality from PDAC arises partly due to late diagnosis resulting from a lack of early-stage biomarkers and due to chemotherapeutic drug resistance, which arises from a highly fibrotic stromal response known as desmoplasia. Desmoplasia alters tissue mechanics, which triggers changes in cell mechanosensing and leads to dysregulated transcriptional activity and disease phenotypes. Hydrogels are effective in vitro models to mimic mechanical changes in tissue mechanics during PDAC progression and to study the influence of these changes on mechanosensitive cell responses. Despite the complex biophysical changes that occur within the PDAC microenvironment, carefully designed hydrogels can very closely recapitulate these properties during PDAC progression. Hydrogels are relatively inexpensive, highly reproducible and can be designed in a humanised manner to increase their relevance for human PDAC studies. In vivo models have some limitations, including species-species differences, high variability, expense and legal/ethical considerations, which make hydrogel models a promising alternative. Here, we comprehensively review recent advancements in hydrogel bioengineering for developing our fundamental understanding of mechanobiology in PDAC, which is critical for informing advanced therapeutics.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 6","pages":"851-870"},"PeriodicalIF":4.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational and experimental advances in liver-on-a-chip technology for cancer research: a systematic review.

IF 4.9

Biophysical reviews Pub Date : 2024-12-14 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01260-z

Violeta Carvalho, Mariana Ferreira, Raquel O Rodrigues, Senhorinha F C F Teixeira, Rui A Lima

{"title":"Computational and experimental advances in liver-on-a-chip technology for cancer research: a systematic review.","authors":"Violeta Carvalho, Mariana Ferreira, Raquel O Rodrigues, Senhorinha F C F Teixeira, Rui A Lima","doi":"10.1007/s12551-024-01260-z","DOIUrl":"10.1007/s12551-024-01260-z","url":null,"abstract":"The integration of numerical simulations with Liver-on-a-Chip (LoC) technology offers an innovative approach for studying liver physiology and pathology, especially in the context of liver cancer. Numerical simulations facilitate the optimization of microfluidic devices' design and deepen the understanding of fluid flow and mass transfer. However, despite significant advancements, challenges such as replicating the full complexity of the liver microenvironment and scaling up for high-throughput screening persist. This systematic review explores the current advancements in LoC devices, with a particular emphasis on their combined use of numerical simulations and experimental studies in liver cancer research. A comprehensive search across multiple databases, including ScienceDirect, Wiley Online Library, Scopus, Springer Link, Web of Science, and PubMed, was conducted to gather relevant literature. Our findings indicate that the combination of both techniques in this field is still rare, resulting in a final selection of 13 original research papers. This review underscores the importance of continued interdisciplinary research to refine these technologies and enhance their application in personalized medicine and cancer therapy. By consolidating existing studies, this review aims to highlight key advancements, identify current challenges, and propose future directions for this rapidly evolving field.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"151-167"},"PeriodicalIF":4.9,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial to the topical issue: the 7th Nanoengineering for Mechanobiology Symposium 2024 Camogli, Genoa, Italy. 时事社论：第七届机械生物学纳米工程研讨会2024卡莫格利，热那亚，意大利。

IF 4.9

Biophysical reviews Pub Date : 2024-12-13 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01262-x

Costanza Giampietro, Aldo Ferrari, Massimo Vassalli

引用次数: 0

Effect of low doses of gamma radiation on cell growth over an electrospun polycaprolactone/zinc acetate scaffold for biomedical applications.

IF 4.9

Biophysical reviews Pub Date : 2024-12-09 eCollection Date: 2025-02-01 DOI: 10.1007/s12551-024-01256-9

Naglaa M Ismail, Soheir Korraa, M B S Osman, Eman El Sheikh

{"title":"Effect of low doses of gamma radiation on cell growth over an electrospun polycaprolactone/zinc acetate scaffold for biomedical applications.","authors":"Naglaa M Ismail, Soheir Korraa, M B S Osman, Eman El Sheikh","doi":"10.1007/s12551-024-01256-9","DOIUrl":"10.1007/s12551-024-01256-9","url":null,"abstract":"The objective of the present study was to generate functional biomaterials to repair and re-establish damaged tissues by producing porous biopolymeric PCL/zinc acetate scaffolds using the electrospinning technique and studying the effect of low doses of gamma radiation on cell proliferation. In electrospinning, ultrafine fibers are spun in a high-voltage electrostatic field. The electrospun structure has natural tissue morphology, which is distinguished by high porosity, a broad variety of pore diameters, efficient mechanical qualities, and the ability to promote cell proliferation and adhesion. PCL/zinc acetate scaffold was investigated by scanning electron microscope (SEM) techniques, Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD). Then, they were sterilized by ionizing radiation (gamma radiation) with a dose of 30 KGy for the cell culture part. Scaffold biocompatibility tests were carried out by using Vero cells. Cells grown on scaffolds were irradiated with doses of 0.5, 1, 2.5, and 5 Gy gamma radiation. Cell viability was examined using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, SEM, malondialdehyde (MDA), and nitric oxide (NO) content. The results proved that cell viability was increased after γ-irradiation with 0.5 Gy compared to control (without γ-irradiation), then decreased with other doses (1, 2.5, and 5 Gy), while the dose of 5 Gy caused the least cell viability. Finally, it was concluded that the nanofiber PCL/zinc acetate scaffold could be successfully fabricated by using the electrospinning technique, and it was biocompatible with Vero cells.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 1","pages":"139-149"},"PeriodicalIF":4.9,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Dimensional reduction and adaptation-development-evolution relation in evolved biological systems. 修正：进化生物系统中的降维与适应-发展-进化关系。

IF 4.9

Biophysical reviews Pub Date : 2024-11-20 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01255-w

Kunihiko Kaneko

引用次数: 0

Tracing the birth and intrinsic disorder of loops and domains in protein evolution. 追踪蛋白质进化中环和结构域的诞生和内在紊乱。

IF 4.9

Biophysical reviews Pub Date : 2024-11-20 eCollection Date: 2024-12-01 DOI: 10.1007/s12551-024-01251-0

Gustavo Caetano-Anollés, Fizza Mughal, M Fayez Aziz, Kelsey Caetano-Anollés

{"title":"Tracing the birth and intrinsic disorder of loops and domains in protein evolution.","authors":"Gustavo Caetano-Anollés, Fizza Mughal, M Fayez Aziz, Kelsey Caetano-Anollés","doi":"10.1007/s12551-024-01251-0","DOIUrl":"10.1007/s12551-024-01251-0","url":null,"abstract":"Protein loops and structural domains are building blocks of molecular structure. They hold evolutionary memory and are largely responsible for the many functions and processes that drive the living world. Here, we briefly review two decades of phylogenomic data-driven research focusing on the emergence and evolution of these elemental architects of protein structure. Phylogenetic trees of domains reconstructed from the proteomes of organisms belonging to all three superkingdoms and viruses were used to build chronological timelines describing the origin of each domain and its embedded loops at different levels of structural abstraction. These timelines consistently recovered six distinct evolutionary phases and a most parsimonious evolutionary progression of cellular life. The timelines also traced the birth of domain structures from loops, which allowed to model their growth ab initio with AlphaFold2. Accretion decreased the disorder of the growing molecules, suggesting disorder is molecular size-dependent. A phylogenomic survey of disorder revealed that loops and domains evolved differently. Loops were highly disordered, disorder increased early in evolution, and ordered and moderate disordered structures were derived. Gradual replacement of loops with α-helix and β-strand bracing structures over time paved the way for the dominance of more disordered loop types. In contrast, ancient domains were ordered, with disorder evolving as a benefit acquired later in evolution. These evolutionary patterns explain inverse correlations between disorder and sequence length of loops and domains. Our findings provide a deep evolutionary view of the link between structure, disorder, flexibility, and function.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 6","pages":"723-735"},"PeriodicalIF":4.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0