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Force generation and resistance in human mitosis.
IF 4.9
Biophysical reviews Pub Date : 2024-09-28 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01235-0
Colleen C Caldwell, Tinka V M Clement, Gijs J L Wuite
{"title":"Force generation and resistance in human mitosis.","authors":"Colleen C Caldwell, Tinka V M Clement, Gijs J L Wuite","doi":"10.1007/s12551-024-01235-0","DOIUrl":"https://doi.org/10.1007/s12551-024-01235-0","url":null,"abstract":"<p><p>Since the first observations of chromosome segregation over 150 years ago, efforts to observe the forces that drive mitosis have evolved alongside advances in microscopy. The mitotic spindle acts as the major generator of force through the highly regulated polymerization and depolymerization of microtubules as well as associated motor proteins. Centromeric chromatin, along with associated proteins including cohesin and condensin, is organized to resist these forces and ensure accurate chromosome segregation. Microtubules and centromeric chromatin join at the kinetochore, a complex protein superstructure. Ongoing research into the forces generated at the kinetochore-microtubule interface has resulted in a range of estimates for forces necessary to separate chromosomes, from tens to hundreds of piconewtons. Still, the exact magnitude and regulation of these forces remain areas of continuing investigation. Determining the precise forces involved in chromosome segregation is hindered by limitations of current measurement techniques, but advances such as optical tweezers combined with fluorescence microscopy are promising for future research.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"551-562"},"PeriodicalIF":4.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent advances in label-free imaging techniques based on nonlinear optical microscopy to reveal the heterogeneity of the tumor microenvironment.
IF 4.9
Biophysical reviews Pub Date : 2024-09-28 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01229-y
Ishita Chakraborty, Nirmal Mazumder, Ankur Gogoi, Ming-Chi Chen, Guan Yu Zhuo
{"title":"Recent advances in label-free imaging techniques based on nonlinear optical microscopy to reveal the heterogeneity of the tumor microenvironment.","authors":"Ishita Chakraborty, Nirmal Mazumder, Ankur Gogoi, Ming-Chi Chen, Guan Yu Zhuo","doi":"10.1007/s12551-024-01229-y","DOIUrl":"https://doi.org/10.1007/s12551-024-01229-y","url":null,"abstract":"<p><p>The tumor microenvironment (TME) is a complex and dynamic network that significantly influences cancer progression. Understanding its intricate components, including the extracellular matrix (ECM), stromal cells, immune cells, and vascular endothelial cells, is crucial for developing effective cancer therapies. Conventional diagnostic methods, while essential, have limitations in sensitivity, specificity, and invasiveness. Label-free multimodal nonlinear optical (MNLO) microscopy offers a promising alternative, enabling detailed imaging without external labels. Techniques such as second harmonic generation (SHG), third harmonic generation (THG), coherent anti-Stokes Raman scattering (CARS), and two-photon fluorescence (TPF) provide complementary insights into the TME. SHG is particularly effective for imaging collagen fibers, while CARS highlights lipid-rich structures, and THG and TPF offer high-resolution imaging of cellular and subcellular structures. These modalities reveal crucial information about tumor progression, including changes in collagen organization and lipid metabolism, and allow for the study of cellular interactions and ECM remodeling. Multimodal setups, combining SHG, CARS, THG, and TPF, enable comprehensive analysis of the TME, facilitating the identification of early-stage cancerous changes and tracking of tumor progression. Despite the advantages of MNLO microscopy, such as reduced photodamage and the ability to image live tissues, challenges remain, including the complexity and cost of the setups. Addressing these challenges through technological advancements and optimization can enhance the applicability of MNLO microscopy in clinical diagnostics and cancer research, ultimately contributing to improved cancer diagnosis, prognosis, and treatment strategies.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"581-590"},"PeriodicalIF":4.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A commentary on the 21st IUPAB/62ND BSJ Congress at Kyoto June 24-28, 2024.
IF 4.9
Biophysical reviews Pub Date : 2024-09-26 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01237-y
Manuel Prieto
{"title":"A commentary on the 21st IUPAB/62ND BSJ Congress at Kyoto June 24-28, 2024.","authors":"Manuel Prieto","doi":"10.1007/s12551-024-01237-y","DOIUrl":"https://doi.org/10.1007/s12551-024-01237-y","url":null,"abstract":"","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"501"},"PeriodicalIF":4.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efforts for younger generations: the Biophysical Society of Japan and IUPAB2024.
IF 4.9
Biophysical reviews Pub Date : 2024-09-26 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01227-0
Satoshi Takahashi
{"title":"Efforts for younger generations: the Biophysical Society of Japan and IUPAB2024.","authors":"Satoshi Takahashi","doi":"10.1007/s12551-024-01227-0","DOIUrl":"https://doi.org/10.1007/s12551-024-01227-0","url":null,"abstract":"<p><p>A personal note from the 2023-2024 President of the Biophysical Society of Japan (BSJ) on the role and activities of the BSJ in preparation for the 2024 IUPAB Congress in Kyoto.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"503-504"},"PeriodicalIF":4.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cofactor recycling strategies for secondary metabolite production in cell-free protein expression systems.
IF 4.9
Biophysical reviews Pub Date : 2024-09-26 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01234-1
Yutong Zou, Constance B Bailey
{"title":"Cofactor recycling strategies for secondary metabolite production in cell-free protein expression systems.","authors":"Yutong Zou, Constance B Bailey","doi":"10.1007/s12551-024-01234-1","DOIUrl":"https://doi.org/10.1007/s12551-024-01234-1","url":null,"abstract":"<p><p>Cell-free protein synthesis (CFPS) has emerged as an attractive platform for biotechnology and synthetic biology due to its numerous advantages to cell-based technologies for specific applications. CFPS can be faster, less sensitive to metabolite toxicity, and amenable to systems that are not easily genetically manipulated. Due to these advantages, a promising application of CFPS is to characterize biosynthetic gene clusters, particularly those harbored within the genomes of microorganisms that generate secondary metabolites, otherwise known as natural products. In the postgenomic era, genome sequencing has revealed an incredible wealth of metabolic diversity. However, far more of these pathways are termed \"cryptic,\" i.e., unable to be produced under standard laboratory conditions than have been characterized. A major barrier to characterizing these cryptic natural products using CFPS is that many of these pathways require utilization of complex cofactors, many of which to date are not recycled efficiently or in an economically viable fashion. In this perspective, we outline strategies to regenerate cofactors relevant to secondary metabolite production in CFPS. This includes adenosine 5'-triphosphate, coenzyme A, redox cofactors (iron-sulfur clusters, nicotinamide adenine dinucleotide phosphate, flavin adenine dinucleotide), all of which play a crucial role in important biosynthetic enzymes. Such advances in cofactor recycling enable continuous production of complex metabolites in CFPS and expand the utility of this emergent platform.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"591-603"},"PeriodicalIF":4.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prebiotic chemistry and protocells during the faint young sun event.
IF 4.9
Biophysical reviews Pub Date : 2024-09-26 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01223-4
Navaniswaran Tharumen, Mahendran Sithamparam, Tony Z Jia, Kuhan Chandru
{"title":"Prebiotic chemistry and protocells during the faint young sun event.","authors":"Navaniswaran Tharumen, Mahendran Sithamparam, Tony Z Jia, Kuhan Chandru","doi":"10.1007/s12551-024-01223-4","DOIUrl":"https://doi.org/10.1007/s12551-024-01223-4","url":null,"abstract":"<p><p>The sun generates light and heat for life on Earth to flourish. However, during the late Hadean-early Archean epoch on Earth, the \"faint young sun\" (FYS) was less luminous, influencing prebiotic chemistry and, by extension, the origins of life (OoL). However, higher levels of ultraviolet (UV) radiation from the FYS, especially UV-C, due to the lack of an ozone layer, would likely have impacted the assembly, stability, persistence, and functions of prebiotic cellular precursors, i.e., protocells. Consequently, it is essential to study how such UV-C radiation would have affected the synthesis and stability of prebiotically relevant molecules and protocells to better understand the plausibility of the OoL during the FYS period. In this letter, we introduce unanswered questions surrounding the structure and stability of protocells under UV-C radiation. Such a perspective may be vital to exoplanetary systems orbiting other stars, enhancing our understanding of life's potential beyond our solar system.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"651-654"},"PeriodicalIF":4.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Overview of the super-resolution and advanced microscopy imaging session at the 21st IUPAB and the 62nd Biophysics Society of Japan joint congress.
IF 4.9
Biophysical reviews Pub Date : 2024-09-24 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01228-z
Takanobu A Katoh, Elizabeth Hinde
{"title":"Overview of the super-resolution and advanced microscopy imaging session at the 21st IUPAB and the 62nd Biophysics Society of Japan joint congress.","authors":"Takanobu A Katoh, Elizabeth Hinde","doi":"10.1007/s12551-024-01228-z","DOIUrl":"https://doi.org/10.1007/s12551-024-01228-z","url":null,"abstract":"<p><p>Recent advancements in microscopic techniques have significantly progressed, with improvements in fundamental parameters such as resolution, as well as the emergence of novel imaging techniques for measuring cellular information. In this session, six invited speakers introduced recent advancements in super-resolution and advanced microscopy imaging, covering both the development of novel microscopy techniques and their biological applications.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"547-550"},"PeriodicalIF":4.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Commentary: Biophysics and Physicobiology, the official international journal of the Biophysical Society of Japan.
IF 4.9
Biophysical reviews Pub Date : 2024-09-20 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01225-2
Haruki Nakamura
{"title":"Commentary: <i>Biophysics and Physicobiology</i>, the official international journal of the Biophysical Society of Japan.","authors":"Haruki Nakamura","doi":"10.1007/s12551-024-01225-2","DOIUrl":"https://doi.org/10.1007/s12551-024-01225-2","url":null,"abstract":"<p><p>This Commentary outlines recent advances of <i>Biophysics and Physicobiology</i> (<i>BPPB</i>), the official international peer-reviewed journal published by the Biophysical Society of Japan (BSJ), a member of the International Union of Pure and Applied Biophysics (IUPAB). In June 2024, BSJ hosted the 21st IUPAB Congress in Kyoto, which was attended by over 1900 participants from 52 countries and regions. The role of <i>BPPB</i> as an open-access (OA) journal within the framework of the future Japanese governmental OA policy is also discussed.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"505-507"},"PeriodicalIF":4.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the dynamics of allostery through multi-dimensional crystallography.
IF 4.9
Biophysical reviews Pub Date : 2024-09-19 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01224-3
C E Hatton, P Mehrabi
{"title":"Exploring the dynamics of allostery through multi-dimensional crystallography.","authors":"C E Hatton, P Mehrabi","doi":"10.1007/s12551-024-01224-3","DOIUrl":"https://doi.org/10.1007/s12551-024-01224-3","url":null,"abstract":"<p><p>By delving into the applications, methodologies, and case studies of multi-dimensional crystallography, whereby time and temperature are varied in the context of allostery, this review aims to elucidate the intricate interplay between structure, dynamics, and function in allosteric proteins. As the field of structural biology continues to advance, integrating multi-dimensional approaches promises to unlock new frontiers in our quest to decipher the molecular mechanisms governing life.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"563-570"},"PeriodicalIF":4.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Data Science for Integrated Dynamic Structural Biology-the 21st IUPAB Congress session summary commentary.
IF 4.9
Biophysical reviews Pub Date : 2024-09-02 eCollection Date: 2024-10-01 DOI: 10.1007/s12551-024-01222-5
Florence Tama, Jianhan Chen
{"title":"Data Science for Integrated Dynamic Structural Biology-the 21st IUPAB Congress session summary commentary.","authors":"Florence Tama, Jianhan Chen","doi":"10.1007/s12551-024-01222-5","DOIUrl":"10.1007/s12551-024-01222-5","url":null,"abstract":"<p><p>The session \"Data Science for Integrated Dynamic Structural Biology\" was a notable success at the joint congress of the 21st IUPAB and the 62nd BSJ (Biophysics Society of Japan). This session included four invited talks and one contributed talk, which together delved into recent advancements in computational methods integrating physics, experimental data, and bioinformatics to study the structure and dynamic properties of molecular assemblies and their interactions.</p>","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"16 5","pages":"529-531"},"PeriodicalIF":4.9,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142766198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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