Biophysical reviews最新文献_第10页

Enzyme dynamics-a brief review. 酶动力学——简评。

IF 4.9

Biophysical reviews Pub Date : 2023-06-23 eCollection Date: 2023-06-01 DOI: 10.1007/s12551-023-01070-9

Jeremy R H Tame

引用次数: 0

Quantitative analysis of human hairs and nails. 人体毛发和指甲的定量分析。

IF 4.9

Biophysical reviews Pub Date : 2023-06-20 eCollection Date: 2023-06-01 DOI: 10.1007/s12551-023-01069-2

Varun Bali, Yugal Khajuria, Vidit Maniyar, Pradeep K Rai, Upendra Kumar, Charles Ghany, M A Gondal, Vivek K Singh

{"title":"Quantitative analysis of human hairs and nails.","authors":"Varun Bali, Yugal Khajuria, Vidit Maniyar, Pradeep K Rai, Upendra Kumar, Charles Ghany, M A Gondal, Vivek K Singh","doi":"10.1007/s12551-023-01069-2","DOIUrl":"10.1007/s12551-023-01069-2","url":null,"abstract":"Hair and nails are human biomarkers capable of providing a continuous assessment of the concentrations of elements inside the human body to indicate the nutritional status, metabolic changes, and the pathogenesis of various human diseases. Laser-induced breakdown spectroscopy (LIBS) and X-ray fluorescence (XRF) spectrometry are robust and multi-element analytical techniques able to analyze biological samples of various kinds for disease diagnosis. The primary objective of this review article is to focus on the major developments and advances in LIBS and XRF for the elemental analysis of hair and nails over the last 10-year period. The developments in the qualitative and quantitative analyses of human hair and nail samples are discussed in detail, with special emphasis on the key aspects of elemental imaging and distribution of essential and non-essential elements within the hair and nail tissue samples. Microchemical imaging applications by LIBS and XRF (including micro-XRF and scanning electron microscopy, SEM) are also presented for healthy as well as diseased tissue hair and nail samples in the context of disease diagnosis. In addition, main challenges, prospects, and complementarities of LIBS and XRF toward analyzing human hair and nails for disease diagnosis are also thoroughly discussed here.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"15 3","pages":"401-417"},"PeriodicalIF":4.9,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9742907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical Reviews: a call for nominations to the 2024 Michéle Auger Award. 生物物理评论：呼吁提名2024年米歇尔·奥格奖。

Biophysical reviews Pub Date : 2023-06-19 eCollection Date: 2023-06-01 DOI: 10.1007/s12551-023-01078-1

Damien Hall

引用次数: 0

Physical virology: how physics is enabling a better understanding of recent viral invaders. 物理病毒学：物理学如何让人们更好地了解近期的病毒入侵者。

Biophysical reviews Pub Date : 2023-06-17 eCollection Date: 2023-08-01 DOI: 10.1007/s12551-023-01075-4

Ruana Cardoso-Lima, Ralph Santos-Oliveira, Pedro Filho Noronha Souza, Leandro R S Barbosa, Gijs J L Wuite, Luciana Magalhães Rebelo Alencar

{"title":"Physical virology: how physics is enabling a better understanding of recent viral invaders.","authors":"Ruana Cardoso-Lima, Ralph Santos-Oliveira, Pedro Filho Noronha Souza, Leandro R S Barbosa, Gijs J L Wuite, Luciana Magalhães Rebelo Alencar","doi":"10.1007/s12551-023-01075-4","DOIUrl":"10.1007/s12551-023-01075-4","url":null,"abstract":"The world is frequently afflicted by several viral outbreaks that bring diseases and health crises. It is vital to comprehend how viral assemblies' fundamental components work to counteract them. Determining the ultrastructure and nanomechanical characteristics of viruses from a physical standpoint helps categorize their mechanical characteristics, offers insight into new treatment options, and/or shows weak spots that can clarify methods for medication targeting. This study compiles the findings from studies on the ultrastructure and nanomechanical behavior of SARS-CoV-2, ZIKV (Zika virus), and CHIKV (Chikungunya virus) viral particles. With results that uncovered aspects of the organization and the spatial distribution of the proteins on the surface of the viral particle as well as the deformation response of the particles when applied a recurring loading force, this review aims to provide further discussion on the mechanical properties of viral particles at the nanoscale, offering new prospects that could be employed for designing strategies for the prevention and treatment of viral diseases.Supplementary information: The online version contains supplementary material available at 10.1007/s12551-023-01075-4.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"15 4","pages":"611-623"},"PeriodicalIF":0.0,"publicationDate":"2023-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10560244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical tools to study the oligomerization dynamics of Prx1-class peroxiredoxins. 研究 Prx1 类过氧化还原酶寡聚动态的生物物理工具。

Biophysical reviews Pub Date : 2023-06-15 eCollection Date: 2023-08-01 DOI: 10.1007/s12551-023-01076-3

Sebastián F Villar, Matías N Möller, Ana Denicola

{"title":"Biophysical tools to study the oligomerization dynamics of Prx1-class peroxiredoxins.","authors":"Sebastián F Villar, Matías N Möller, Ana Denicola","doi":"10.1007/s12551-023-01076-3","DOIUrl":"10.1007/s12551-023-01076-3","url":null,"abstract":"Peroxiredoxins (Prx) are ubiquitous, highly conserved peroxidases whose activity depends on catalytic cysteine residues. The Prx1-class of the peroxiredoxin family, also called typical 2-Cys Prx, organize as head-to-tail homodimers containing two active sites. The peroxidatic cysteine CP of one monomer reacts with the peroxide substrate to form sulfenic acid that reacts with the resolving cysteine (CR) of the adjacent subunit to form an intermolecular disulfide, that is reduced back by the thioredoxin/thioredoxin reductase/NADPH system. Although the minimal catalytic unit is the dimer, these Prx oligomerize into (do)decamers. In addition, these ring-shaped decamers can pile-up into high molecular weight structures. Prx not only display peroxidase activity reducing H2O2, peroxynitrous acid and lipid hydroperoxides (antioxidant enzymes), but also exhibit holdase activity protecting other proteins from unfolding (molecular chaperones). Highly relevant is their participation in redox cellular signaling that is currently under active investigation. The different activities attributed to Prx are strongly ligated to their quaternary structure. In this review, we will describe different biophysical approaches used to characterize the oligomerization dynamics of Prx that include the classical size-exclusion chromatography, analytical ultracentrifugation, calorimetry, and also fluorescence anisotropy and lifetime measurements, as well as mass photometry.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"15 4","pages":"601-609"},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editors' Roundup: June 2023. 编辑综述：2023年6月。

IF 4.9

Biophysical reviews Pub Date : 2023-06-15 eCollection Date: 2023-06-01 DOI: 10.1007/s12551-023-01077-2

Gautam Basu, Yuki Sudo, Lawrence Berliner, Konstantin Shaitan, Damien Hall

引用次数: 0

When push comes to shove - RNA polymerase and DNA-bound protein roadblocks. 当事态发展到紧要关头时，核糖核酸聚合酶和脱氧核糖核酸结合蛋白就会成为障碍。

Biophysical reviews Pub Date : 2023-06-10 eCollection Date: 2023-06-01 DOI: 10.1007/s12551-023-01064-7

Nan Hao, Alana J Donnelly, Ian B Dodd, Keith E Shearwin

{"title":"When push comes to shove - RNA polymerase and DNA-bound protein roadblocks.","authors":"Nan Hao, Alana J Donnelly, Ian B Dodd, Keith E Shearwin","doi":"10.1007/s12551-023-01064-7","DOIUrl":"10.1007/s12551-023-01064-7","url":null,"abstract":"In recent years, transcriptional roadblocking has emerged as a crucial regulatory mechanism in gene expression, whereby other DNA-bound obstacles can block the progression of transcribing RNA polymerase (RNAP), leading to RNAP pausing and ultimately dissociation from the DNA template. In this review, we discuss the mechanisms by which transcriptional roadblocks can impede RNAP progression, as well as how RNAP can overcome these obstacles to continue transcription. We examine different DNA-binding proteins involved in transcriptional roadblocking and their biophysical properties that determine their effectiveness in blocking RNAP progression. The catalytically dead CRISPR-Cas (dCas) protein is used as an example of an engineered programmable roadblock, and the current literature in understanding the polarity of dCas roadblocking is also discussed. Finally, we delve into a stochastic model of transcriptional roadblocking and highlight the importance of transcription factor binding kinetics and its resistance to dislodgement by an elongating RNAP in determining the strength of a roadblock.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"15 3","pages":"355-366"},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9736005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Don't go breakin' my heart: cardioprotective alterations to the mechanical and structural properties of reperfused myocardium during post-infarction inflammation. 不要破坏我的心脏：在梗死后炎症期间，再灌注心肌的机械和结构特性发生了心脏保护性改变。

IF 4.9

Biophysical reviews Pub Date : 2023-06-10 eCollection Date: 2023-06-01 DOI: 10.1007/s12551-023-01068-3

Daniel P Pearce, Mark T Nemcek, Colleen M Witzenburg

{"title":"Don't go breakin' my heart: cardioprotective alterations to the mechanical and structural properties of reperfused myocardium during post-infarction inflammation.","authors":"Daniel P Pearce, Mark T Nemcek, Colleen M Witzenburg","doi":"10.1007/s12551-023-01068-3","DOIUrl":"10.1007/s12551-023-01068-3","url":null,"abstract":"Myocardial infarctions (MIs) kickstart an intense inflammatory response resulting in extracellular matrix (ECM) degradation, wall thinning, and chamber dilation that leaves the heart susceptible to rupture. Reperfusion therapy is one of the most effective strategies for limiting adverse effects of MIs, but is a challenge to administer in a timely manner. Late reperfusion therapy (LRT; 3 + hours post-MI) does not limit infarct size, but does reduce incidences of post-MI rupture and improves long-term patient outcomes. Foundational studies employing LRT in the mid-twentieth century revealed beneficial reductions in infarct expansion, aneurysm formation, and left ventricle dysfunction. The mechanism by which LRT acts, however, is undefined. Structural analyses, relying largely on one-dimensional estimates of ECM composition, have found few differences in collagen content between LRT and permanently occluded animal models when using homogeneous samples from infarct cores. Uniaxial testing, on the other hand, revealed slight reductions in stiffness early in inflammation, followed soon after by an enhanced resistance to failure for cases of LRT. The use of one-dimensional estimates of ECM organization and gross mechanical function have resulted in a poor understanding of the infarct's spatially variable mechanical and structural anisotropy. To resolve these gaps in literature, future work employing full-field mechanical, structural, and cellular analyses is needed to better define the spatiotemporal post-MI alterations occurring during the inflammatory phase of healing and how they are impacted following reperfusion therapy. In turn, these studies may reveal how LRT affects the likelihood of rupture and inspire novel approaches to guide scar formation.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"15 3","pages":"329-353"},"PeriodicalIF":4.9,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9744329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supramolecular assemblies from antimony(V) complexes for the treatment of leishmaniasis. 用于治疗利什曼病的锑(V)复合物超分子组装。

Biophysical reviews Pub Date : 2023-06-06 eCollection Date: 2023-08-01 DOI: 10.1007/s12551-023-01073-6

Cynthia Demicheli, Virgínia M R Vallejos, Juliane S Lanza, Guilherme S Ramos, Bruno R Do Prado, Sébastien Pomel, Philippe M Loiseau, Frédéric Frézard

{"title":"Supramolecular assemblies from antimony(V) complexes for the treatment of leishmaniasis.","authors":"Cynthia Demicheli, Virgínia M R Vallejos, Juliane S Lanza, Guilherme S Ramos, Bruno R Do Prado, Sébastien Pomel, Philippe M Loiseau, Frédéric Frézard","doi":"10.1007/s12551-023-01073-6","DOIUrl":"10.1007/s12551-023-01073-6","url":null,"abstract":"The pentavalent meglumine antimoniate (MA) is still a first-line drug in the treatment of leishmaniasis in several countries. As an attempt to elucidate its mechanism of action and develop new antimonial drugs with improved therapeutic profile, Sb(V) complexes with different ligands, including β-cyclodextrin (β-CD), nucleosides and non-ionic surfactants, have been studied. Interestingly, Sb(V) oxide, MA, its complex with β-CD, Sb(V)-guanosine complex and amphiphilic Sb(V) complexes with N-alkyl-N-methylglucamide, have shown marked tendency to self-assemble in aqueous solutions, forming nanoaggregates, hydrogel or micelle-like nanoparticles. Surprisingly, the resulting assemblies presented in most cases slow dissociation kinetics upon dilution and a strong influence of pH, which impacted on their pharmacokinetic and therapeutic properties against leishmaniasis. To explain this unique property, we raised the hypothesis that multiple pnictogen bonds could contribute to the formation of these assemblies and their kinetic of dissociation. The present article reviews our current knowledge on the structural organization and physicochemical characteristics of Sb-based supramolecular assemblies, as well as their pharmacological properties and potential for treatment of leishmaniasis. This review supports the feasibility of the rational design of new Sb(V) complexes with supramolecular assemblies for the safe and effective treatment of leishmaniasis.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"15 4","pages":"751-765"},"PeriodicalIF":0.0,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10542218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

(Dys)functional insights into nucleic acids and RNA-binding proteins modulation of the prion protein and α-synuclein phase separation. (核酸和 RNA 结合蛋白调节朊病毒蛋白和 α-突触核蛋白相分离的（非）功能性见解。

IF 4.9

Biophysical reviews Pub Date : 2023-06-06 eCollection Date: 2023-08-01 DOI: 10.1007/s12551-023-01067-4

Yraima Cordeiro, Maria Heloisa O Freire, Adalgisa Felippe Wiecikowski, Mariana Juliani do Amaral

{"title":"(Dys)functional insights into nucleic acids and RNA-binding proteins modulation of the prion protein and α-synuclein phase separation.","authors":"Yraima Cordeiro, Maria Heloisa O Freire, Adalgisa Felippe Wiecikowski, Mariana Juliani do Amaral","doi":"10.1007/s12551-023-01067-4","DOIUrl":"10.1007/s12551-023-01067-4","url":null,"abstract":"Prion diseases are prototype of infectious diseases transmitted by a protein, the prion protein (PrP), and are still not understandable at the molecular level. Heterogenous species of aggregated PrP can be generated from its monomer. α-synuclein (αSyn), related to Parkinson's disease, has also shown a prion-like pathogenic character, and likewise PrP interacts with nucleic acids (NAs), which in turn modulate their aggregation. Recently, our group and others have characterized that NAs and/or RNA-binding proteins (RBPs) modulate recombinant PrP and/or αSyn condensates formation, and uncontrolled condensation might precede pathological aggregation. Tackling abnormal phase separation of neurodegenerative disease-related proteins has been proposed as a promising therapeutic target. Therefore, understanding the mechanism by which polyanions, like NAs, modulate phase transitions intracellularly, is key to assess their role on toxicity promotion and neuronal death. Herein we discuss data on the nucleic acids binding properties and phase separation ability of PrP and αSyn with a special focus on their modulation by NAs and RBPs. Furthermore, we provide insights into condensation of PrP and/or αSyn in the light of non-trivial subcellular locations such as the nuclear and cytosolic environments.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"15 4","pages":"577-589"},"PeriodicalIF":4.9,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10191067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0